Comparative Evaluation of Antiulcer Activity of Ficus religiosa (Stem Bark) Extracts Prepared by Different Methods of Extraction

2020 ◽  
Vol 6 (4) ◽  
pp. 351-359
Author(s):  
Sunita Panchawat ◽  
Joohee Pradhan
Keyword(s):  
Extraction Method ◽  
Soxhlet Extraction ◽  
Stem Bark ◽  
Antiulcer Activity ◽  
Ulcer Index ◽  
Ficus Religiosa ◽  
Standard Drug ◽  
Hydroalcoholic Extract ◽  
Acute Toxicity Study ◽  
Assisted Extraction

Background: The stem bark of Ficus religiosa L. (Family-Moraceae) is traditionally used to treat gonorrhea, diarrhea, dysentery, leucorrhea, menorrhagia, vaginal and other urogenical disorders, ulcers and gastrohelcosis. Objective: The study was aimed at comparing antiulcer activity (ethanol induced gastric ulcer) for stem bark extracts of Ficus religiosa Linn. prepared by different extraction methods viz. soxhlet, ultrasonication and microwave assisted extraction. Methods: The powdered drug material was extracted by soxhlet (time 48 hours., temperature 60°C and solvent 60% ethanol), Ultrasonic (90 min., 50°C and acetone as solvent)and Microwave (3 min., 60°C and acetone as solvent) assisted extraction method. The acute toxicity study was carried out in adult female albino rats by “fix dose” method of OECD guideline no. 420 for selection of doses. Antiulcerogenic effect of prepared extracts (200mg/kg) was evaluated by ethanol induced ulcer method using omeprazole (40 mg/kg) as standard drug and absolute alcohol 99.5% as necrotizing agent. Result and Conclusion: Acute toxicity study did not show any significant toxicity signs and extracts were said to be practically non-toxic at tested dose level of 2000 mg/kg, b.w. The best antiulcer activity was shown by the hydroalcoholic extract prepared by soxhlet extraction method. Administration of ethanol produced significant ulcer index (41.00 ± 1.75) in the control group. Standard drug omeprazole (40 mg/kg) produced percentage protection of 70.31%. Among all three extracts, Ulcer index is significantly (p<0.001) protected by hydroalcoholic extract (Soxhlet Extraction Method) with percentage protection 52.02%.

Evaluation of the Antiulcer Activity of Ethanolic Leave Extracts of Saraca indica against Ethanol, Pylorus Ligature and Indomethacin Induced Ulcer in Albino Rats

2020 ◽  
Vol 16 (8) ◽  
pp. 1191-1196
Author(s):  
Pritt Verma ◽  
Shravan K. Paswan ◽  
Vishal K. Vishwakarma ◽  
Priyanshi Saxena ◽  
Chandana V. Rao ◽  
...  
Keyword(s):  
Mucosal Injury ◽  
Gastric Volume ◽  
Gastric Ulcers ◽  
Albino Rats ◽  
Antiulcer Activity ◽  
Cellulose Solution ◽  
Total Acidity ◽  
Ulcer Index ◽  
Standard Drug ◽  
High Doses

Background: To evaluate the antiulcer activity of ethanolic leaves extract of Saraca indica against ethanol, pylorus ligature and indomethacin in albino rats. Materials and Methods: Ulcer was produced by ethanol, pylorus ligature and indomethacin in albino rats. Five groups (n=6) of rats were orally pre-treated with carboxymethyl cellulose solution, and ranitidine (80 mg/kg) respectively. In ethanol induced ulcer, the animals were treated with 200 and 400 mg/kg b.w. ethanolic leave extracts of Saraca indica in 0.3% CMC solution, 60 minutes before oral administration of absolute ethanol to produce gastric mucosal injury. In indomethacin induced ulcer, the drug was administered orally at the dose of 30 mg/kg b.w. After 7-9 hours of administration of indomethacin (30 mg/kg); the animals were sacrificed with high doses of anesthesia. In the pylorus ligature method, volume of free acidity, gastric secretion, pH and total acidity were estimated. In all three models, the ulcer index and % protection were estimated. Results: The anti ulcer activity of ethanolic leave extracts of Saraca indica in ethanol, indomethacin and pylorus ligature models is evident from the significant (P<0.001) reduction in ulcer index. In pylorus ligature model, significant (P<0.001) reduction in total acidity gastric volume and increase in pH were observed when compared with the standard drug. Conclusion: Ethanolic leave extracts of Saraca indica were found to be significantly protective against ethanol, indomethacin and pylorus ligature induced gastric ulcers in the experimental albino rats. The result obtained suggest that ethanolic leave extracts of Saraca indica possesses significant anti-ulcer activity.

ANTIULCER AND ANTI-INFLAMMATORY ACTIVITIES OF HYDROALCOHOLIC EXTRACTS OF STEM BARK AND LEAF OF ANOGEISSUS PENDULA EDGEW

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (01) ◽  
pp. 41-46
Author(s):  
Deeksha Singh ◽  
◽  
Amit Nayak ◽  
Rakesh Yadav ◽  
Uttam Singh Baghel ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Stem Bark ◽  
Inflammatory Activity ◽  
Antiulcer Activity ◽  
Paw Edema ◽  
Ulcer Index ◽  
Pylorus Ligation ◽  
Anti Inflammatory ◽  
Anogeissus Pendula

Anogeissus pendula Edgew has been reported to be used in gastric disorder even though no attempt has been made to evaluate the same. The present study was designed to evaluate the hydroalcoholic extracts of stem bark and leaves for in vivo acute antiulcer and anti-inflammatory activities. Antiulcer activity was studied by pylorus ligation induced ulcers while anti-inflammatory activities was studied by carrageenan induced paw edema. The extract of stem bark at 200 and 400 mg/kg b. w., significantly (P<0.05) reduced ulcer index whereas extracts of both stem bark and leaves significantly (P<0.001) reduced paw diameter at the fifth hour. The hydroalcholic extract of stem bark of A. pendula possesses more significant antiulcer and anti-inflammatory activity than the hydroalcholic extract of leaves may be due to the presence of higher amount of phenolic compounds.

scholarly journals A Original Article ANALGESIC ACTIVITY OF BARK AND LEAVES OF FICUS RELIGIOSA L. FROM NEPAL

2020 ◽  
pp. 32-35
Author(s):  
DHARMA RAJ MARASINI ◽  
JITENDRA PANDEY ◽  
LAXMAN PAUDEL SHARMA ◽  
LAXMI PAUDEL ◽  
ROSHNI GYAWALI ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Analgesic Activity ◽  
Test Group ◽  
Ethanolic Extract ◽  
Stem Bark ◽  
Latency Time ◽  
Hot Plate ◽  
Ficus Religiosa ◽  
Standard Drug ◽  
Ethanolic Extracts

Objective: Because of adverse side effects, caused by NSAIDs, tolerance, and dependence induced by opiates, the use of these analgesic agents has not been successful in all cases. Therefore, alternative analgesic drugs from plant sources are the new target now days. The objective of this study was to evaluate the analgesic activity of ethanolic extracts of stem barks and leaves of Ficus religiosa. Methods: The analgesic activity of ethanolic extract of stem barks and leaves was evaluated in the Swiss albino mice model using acetic acid-induced writing response and Eddy’s hot plate method. Analgesic activity was demonstrated with the percentage inhibition of acetic acid induced writings and the percentage increased in latency time of paw licking. The potency of test extracts was compared with standard drug, Diclofenac. Results: Ethanolic extract of leaves and bark of F. religiosa showed potential analgesic activity from both methods. From Eddy’s hot plate model, it was observed that the percentage of increased latency time at 90 min by ethanolic extract of leaves and stem bark was found to be 70.81 % (8.54 min) and 70.78 % (8.53 min) respectively at a dose of 400 mg/kg. Both of these results are statistically significant (p<0.05) as compared to the test group. Furthermore, both of these extracts showed the dose-dependent and time-dependent increased in latency time and these results are compared to that of standard drug Diclofenac. Similarly, ethanolic extract of leaves and stem at 400 mg/kg significantly inhibited the number of writhings induced by acetic acid. The percentage inhibition of writhings by ethanolic extract of leaves at a dose of 400 mg/kg was 68.47 % which was similar to that of standard drug Diclofenac (68.47 %). However, ethanolic extract of bark showed relatively lower percentage inhibition (60.79 %) as compared to leaf extract and standard, but the result was significant as compared to that of the test group (p<0.05). Conclusion: Ethanolic extracts of F. religiosa stem bark and leaf possess both central and peripheral analgesic properties and these effects may be beneficial for the management of pain.

scholarly journals Gastroprotective Effect of Freeze Dried Stripped Snakehead Fish (Channa striata Bloch.) Aqueous Extract against Aspirin Induced Ulcerogenesis in Pylorus Ligated Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Mohammed Safwan Ali Khan ◽  
Abdul Manan Mat Jais ◽  
Javeed Hussain ◽  
Faiza Siddiqua ◽  
A. Gopala Reddy ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Gastric Juice ◽  
Aqueous Extract ◽  
Antiulcer Activity ◽  
Fresh Water Fish ◽  
Ulcer Index ◽  
Standard Drug ◽  
Freeze Dried ◽  
Wide Range ◽  
Channa Striata

Channa striata (Bloch.) is a fresh water fish belonging to the family Channidae. The stripped snakehead fish possesses wide range of medicinal properties. In view of traditional use of C. striata for wound healing, the present study was undertaken to investigate the beneficial effects of orally administered freeze dried aqueous extract of Channa striata (AECS) in experimentally induced gastric ulcers in Wistar rats. Aspirin induced ulcerogenesis in pyloric ligation model was used for the assessment of antiulcer activity and Ranitidine (50 mg/kg) was employed as the standard drug. The various gastric parameters like volume of gastric juice, pH, free and total acidities, ulcer index, and levels of antioxidant enzymes like catalase, superoxide dismutase, and lipid peroxidation marker malondialdehyde were determined. AECS at concentrations of 40% and 50% w/v significantly decreased the volume of gastric juice and increased the levels of catalase while considerable decrease in free and total acidities and increase in superoxide dismutase were observed with the treatment of standard drug and AECS (50% w/v). All the test doses of AECS markedly decreased ulcer index and malondialdehyde compared to the standard drug whereas AECS 30% w/v did not alter volume of gastric juice, pH, free and total acidities, catalase, and superoxide dismutase. From these findings, it can be concluded that AECS is devoid of acid neutralizing effects at lower doses and possesses antisecretory and antiulcer activities and this could be related to its antioxidant mechanism.

scholarly journals Peptic Ulcer Healing Activity of Fruit Pulp Extract and Seed Oil of Adansonia digitata Linn

2019 ◽  
Vol 11 (05) ◽  
Author(s):  
Kajal Kamalso Malgave ◽  
Nilofar Shahnwaj Naikwade ◽  
Padma Laxmikant Ladda ◽  
Tabbasum S. Shikalgar ◽  
Sudhir S. Patil
Keyword(s):  
Secondary Metabolites ◽  
Seed Oil ◽  
Enzyme Level ◽  
Histopathological Study ◽  
Antiulcer Activity ◽  
Marker Enzyme ◽  
Fruit Pulp ◽  
Adansonia Digitata ◽  
Ulcer Index ◽  
Standard Drug

Adansonia digitata (AD) Linn has been used to cure PU in Ayurveda but its efficacy has not been validated. The current study was so carried out to evaluate the antiulcer activity of ethanolic extract of Adansonia digitata fruit pulp (ADFP), n hexane extract of Adansonia digitata seed oil (ADSO) and their combination (ADFP+ADSO) in rats. The effect of AD on gastric ulcer in pylorus ligation induced and ethanol induced models was studied using doses [ADFO (500 mg/kg), ADSO (300 mg/kg) and combination of ADFP and ADSO] for 10 days. Omeprazole (10 mg/kg) were used as the standard drug. Depending on the model, outcomes measures were gastric volume, pH, free acidity, total acidity, ulcer index, percentage inhibition of ulcer index, protein, pepsin, mucus, antioxidant marker enzyme level (Superoxide dismutase, Catalase, Lipid peroxidation), morphological and Histopathological study. The result obtained with combination was set up near to the standard drug and consequence showed that the combination of ADFP and ADSO was found to be more effective than the individual extract of AD. The outcomes were statistically evaluated with the one-way ANOVA followed by the test of Dennett’s‘t’. The secondary-metabolites such as flavonoids, proteins, saponins, tannins, phenols, terpenoids, alkaloids and Fatty acids (palmitic acid, oleic acid, linoleic acid) are potent as antioxidant, antiulcer and anti-inflammatory. The finding of this reading confirmed that AD has antiulcer activity due to 1 or more of the secondary-metabolites present in it. Therefore, this study validates its antiulcer use in Ayurveda. Future investigation on separation of specific phytochemicals and elucidate MOA are needed.

scholarly journals Anti-infective efficacy of Psidium guajava L. leaves against certain pathogenic bacteria

F1000Research ◽  
2019 ◽  
Vol 8 ◽  
pp. 12
Author(s):  
Pooja Patel ◽  
Chinmayi Joshi ◽  
Tannaz Birdi ◽  
Vijay Kothari
Keyword(s):  
Extraction Method ◽  
Pathogenic Bacteria ◽  
Water Extract ◽  
Psidium Guajava ◽  
Extraction Methods ◽  
Hydroalcoholic Extract ◽  
C Elegans ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Infective Activity

Water extracts of Psidium guajava leaves prepared by three different extraction methods were compared with respect to their anti-infective activity against Pseudomonas aeruginosa and Staphylococcus aureus in the nematode host Caenorhabditis elegans. The water extract prepared by Microwave Assisted Extraction method was found to have better anti-infective activity, and its activity was further compared with hydroalcoholic extract prepared using the same extraction method against five different pathogenic bacteria. Both these extracts could attenuate virulence of P. aeruginosa, S. aureus, Serratia marcescens, and Chromobacterium violaceum, towards C. elegans. Anti-infective efficacy of P. guajava leaf extract seems partly to stem from its quorum-modulatory property, as it could modulate production of quorum sensing-regulated pigments in all the susceptible bacteria.

Pharmacological Evaluation of Anti-ulcer Activity of Caesalpinia crista in Rats

2017 ◽  
Vol 10 (4) ◽  
pp. 3772-3779
Author(s):  
Kintu Patel ◽  
Bhagyabhumi Patel ◽  
Alkesh Patel ◽  
Samir Shah
Keyword(s):  
Inflammatory Cells ◽  
Control Group ◽  
Antiulcer Activity ◽  
Standard Group ◽  
Free Acidity ◽  
Rat Stomach ◽  
Ulcer Index ◽  
Standard Drug ◽  
Group Iii ◽  
Group I

In present study, we evaluated the antiulcer activity of the herbal preparation of Caesalpinia crista in rat models.  Experimental animals were divided into four groups. Rats of group I (disease control) treated with normal saline only, group II (standard group) treated with Omeprazole (2 mg/ kg; p.o.), group III and IV served as test groups and were treated with Caesalpinia crista extract (CE) in the dose of 250 mg/ kg and 500 mg/ kg orally respectively. Peptic ulcer was induced by ligating the pyloric portion of rat stomach and was done 45 min after the respective treatment. After 4 hour of pylorus ligation, rats were sacrificed. Parameters like ulcer index, percent ulcer protection, total and free acidity were estimated for evaluation of anti-ulcer activity. Histopathological evaluation was also performed. The aqueous extract of Caesalpinia crista seeds reduced the volume of gastric juice, free acidity, total acidity and ulcer index. It increased the pH of the gastric acid. Histopathology of the rat stomach revealed the presence of lesions and infiltration of inflammatory cells in control group. Moreover, animals treated with test drug and standard drug did not reveal any microscopic lesions. These findings suggest that Caesalpinia crista seeds may have anti-secretory and anti-ulcer activity and may be helpful for ulcer therapy. 

Comparison of Different Extraction Methods for Antioxidant Property of Flavonoids from Pomelo Peel

2013 ◽  
Vol 652-654 ◽  
pp. 443-448 ◽  
Author(s):  
Qiao Mei Ru ◽  
Ru Fan Cai ◽  
Jin Zhe He
Keyword(s):  
Extraction Method ◽  
Antioxidant Activities ◽  
Soxhlet Extraction ◽  
Antioxidant Property ◽  
Extraction Methods ◽  
Antioxidant Ability ◽  
Microwave Assisted ◽  
Pomelo Peel ◽  
Assisted Extraction ◽  
Different Levels

Antioxidant activity of the flavonoids from pomelo (Citrus grandis (L.) Osbeck) peel obtained by conventional soxhlet extraction (CSE), microwave-assisted extraction (MAE) and supercritical carbon dioxide (SC-CO2) extraction were investigated. For measuring antioxidant activities, different methods have been used corresponding to different levels of antioxidant action. The results indicated that the extraction yields of flavonoids by MAE and SC-CO2 extraction were higher than that by CSE method, whereas flavoniods by SC-CO2 extraction exhibited the strongest antioxidant activities. Our results indicated that SC-CO2 extraction method could effectively reserve the antioxidant ability of pomelo peel flavonoids.

scholarly journals Gastro-protective effects of methanol extract of Tephrosia calophylla

2018 ◽  
Vol 8 (6-s) ◽  
pp. 141-145
Author(s):  
C Ramesh ◽  
A. Prameela Rani
Keyword(s):  
Protected Area ◽  
Methanol Extract ◽  
Research Work ◽  
Albino Rats ◽  
Antiulcer Activity ◽  
Total Acidity ◽  
Protective Effects ◽  
Ulcer Index ◽  
Standard Drug ◽  
Soxhlet Apparatus

Objective: The present research work was designed to investigate gastro protective potentials of methanol extract of Tephrosia calophylla. Methods: The aerial parts of Tephrosia calophylla were dried under shade, powdered and deffated with petroleum ether and then marc left over was subjected to methanol extraction using soxhlet apparatus. Antiulcer activity of methanol extract was determined against stress induced and aspirin induced ulcers in experimental animal models. The total number of ulcers formed, ulcer index, percentage inhibition, ulcerated area, protected area, pH and Total acidity were parameters in the study. Results: Methanol extract of Tephrosia calophylla have significantly reduced the total number of ulcers formed, ulcer index, ulcerated area and total acidity in therapeutic groups compare to vehicle control and there by significantly increased percentage inhibition of ulcers and protected area which was evident by significant rise in pH of gastric content. The effect of extracts was dose dependent and results were comparable to that of standard drug omeprazole. Conclusion: The results obtained from the present work suggest that the methanol extract of Tephrosia calophylla possess significant anti-ulcer potentials against experimentally induced ulcers in albino rats. Keywords: Tephrosia calophylla, Anti ulcer activity, Ethanol, Aspirin Ulcer index, pH, total acidity, Percentage inhibition and percentage of protected area.

DEVELOPMENT OF ANTIULCER HERBOMINERAL FORMULATION ALCECARE

INDIAN DRUGS ◽  
2014 ◽  
Vol 51 (02) ◽  
pp. 54-59
Author(s):  
M Prasad ◽  
◽  
R Rajput ◽  
A. Kushwaha ◽  
R. Bharti
Keyword(s):  
Gastric Juice ◽  
Aqueous Extract ◽  
Seed Oil ◽  
Negative Control ◽  
Control Group ◽  
Antiulcer Activity ◽  
Ulcer Index ◽  
Standard Drug ◽  
Calculated Quantity

A herbomineral formulation was developed in suspension form by using the herb, mineral and different excipient and named “Alcecare”. The formulation was prepared by mixing calculated quantity of shatavari aqueous extract, fennel seed oil and praval bhasma to make smooth cream and then other excipients added to form a suspension for the evaluation of antiulcer activity in rats using aspirin model. The anti-ulcer activity of herbomineral formulation showed that the minimum ulcer index was observed with pantoprazole (1.98±0.29) which was nearly comparable with herbomineral formulation (2.85±0.47) and showed significant antiulcer activity compared to negative control with reducing the volume of gastric juice (5.99±0.25, 3.57±0.92, 4.22±0.67 of ulcerated control, standard and herbomineral formulation groups respectively). It was observed that developed herbomineral formulation possess significant antiulcer activity compared to control group and obtained results were nearly comparable with standard drug (pantoprazole).

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