scholarly journals 2,7-Dichlorofluorescein Hydrazide as a New Fluorescent Probe for Mercury Quantification: Application to Industrial Effluents and Polluted Water Samples

2013 ◽  
Vol 2013 ◽  
pp. 1-8
Author(s):  
Sureshkumar Kempahanumakkagari ◽  
Pandurangappa Malingappa ◽  
Gopi Ambikapathi ◽  
Devaraju Kuramkote Shivanna
Keyword(s):  
Fluorescent Probe ◽  
Color Change ◽  
Limit Of Detection ◽  
Industrial Effluents ◽  
Amide Bond ◽  
Polluted Water ◽  
Hydrolytic Cleavage ◽  
Range Limit ◽  
Relative Standard ◽  
Interfering Ions

A new fluorescent probe 2,7-dichlorofluorescein hydrazide for mercury quantification in aqueous medium has been described. It is based on the spirolactam ring opening of colorless and nonfluorescent 2,7-dichlorofluorescein hydrazide induced by Hg2+ions through the hydrolytic cleavage of amide bond to produce green-colored highly fluorescent dichlorofluorescein in alkaline medium. The significant color change of this reagent in the presence of mercury ions can be used as a sensitive naked-eye detector. The working range, limit of detection, and relative standard deviations were found to be 0.2–20 ngmL−1, 0.042 ngmL−1, and 0.69% respectively. The proposed method is free from most of the common interfering ions present in the environmental samples. The developed method has been successfully applied to determine trace level mercury from water, soil, and industrial effluents.

scholarly journals A highly sensitive octopus-like azobenzene fluorescent probe for determination of abamectin B1 in apples

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zhenlong Guo ◽  
YiFei Su ◽  
Kexin Li ◽  
MengYi Tang ◽  
Qiang Li ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Limit Of Detection ◽  
Quantitative Detection ◽  
Fluorescence Signal ◽  
Ionization Mass ◽  
Limit Of Quantification ◽  
Visible Absorption ◽  
Esi Ms ◽  
Relative Standard ◽  
Ft Ir

AbstractThe development of detecting residual level of abamectin B1 in apples is of great importance to public health. Herein, we synthesized a octopus-like azobenzene fluorescent probe 1,3,5-tris (5′-[(E)-(p-phenoxyazo) diazenyl)] benzene-1,3-dicarboxylic acid) benzene (TPB) for preliminary detection of abamectin B1 in apples. The TPB molecule has been characterized by ultraviolet–visible absorption spectrometry, 1H-nuclear magnetic resonance, fourier-transform infrared (FT-IR), electrospray ionization mass spectroscopy (ESI-MS) and fluorescent spectra. A proper determination condition was optimized, with limit of detection and limit of quantification of 1.3 µg L−1 and 4.4 μg L−1, respectively. The mechanism of this probe to identify abamectin B1 was illustrated in terms of undergoing aromatic nucleophilic substitution, by comparing fluorescence changes, FT-IR and ESI-MS. Furthermore, a facile quantitative detection of the residual abamectin B1 in apples was achieved. Good reproducibility was present based on relative standard deviation of 2.2%. Six carboxyl recognition sites, three azo groups and unique fluorescence signal towards abamectin B1 of this fluorescent probe demonstrated reasonable sensitivity, specificity and selectivity. The results indicate that the octopus-like azobenzene fluorescent probe can be expected to be reliable for evaluating abamectin B1 in agricultural foods.

scholarly journals Full validation of an electrothermal atomic absorption assay for zinc in hepatic tissue using a fast sample preparation procedure

2006 ◽  
Vol 20 (2) ◽  
pp. 81-87
Author(s):  
Pedro Eduardo Fröehlich ◽  
Raquel Borges Pinto ◽  
André Castagna Wortmann ◽  
Richard Borba Magalhães ◽  
Ana Cláudia Reis Schneider ◽  
...  
Keyword(s):  
Clinical Trials ◽  
Limit Of Detection ◽  
Bovine Liver ◽  
Hepatic Tissue ◽  
Range Limit ◽  
Experimental Protocol ◽  
Limit Of Quantification ◽  
Relative Standard ◽  
Sample Preparation Procedure ◽  
Analytical Assay

The objective of this work was to develop and fully validate an analytical assay to quantify zinc in hepatic tissue. The procedure should be as simple and fast as possible in order to avoid sample contamination. The amount of sample used should also reflect the sample size usually obtained in clinical biopsies, which are about 3–4 mg at most. The validation protocol is in accordance to international guidelines, such as ICH and FDA. The parameters evaluated were precision, accuracy, range, limit of detection and limit of quantification. The method was evaluated in the 2.0–32.0 parts per billion (μg/l) range. Under the described conditions intra and inter day precision of the three levels of quality controls were lower than 9.06 and 5.27, respectively, expressed as relative standard deviation (RSD). The accuracy ranged from 86.35 to 114.71%. Limit of detection and limit of quantification were 0.60 and 2.0 μg/l, respectively. Fresh bovine liver samples were used in order to evaluate the clinical procedure used to collect biopsies. According to the results and experimental protocol, the method is fully validated and ready to use in clinical trials involving zinc quantitation using hepatic samples as small as 2.00 mg of dry tissue.

scholarly journals POSSIBILITY OF THE SPECTROPHOTOMETRIC DETERMINATION OF EUROPIUM BY MEANS OF ARSENAZO III

2013 ◽  
Vol 12 (2) ◽  
pp. 93-99 ◽  
Author(s):  
Jozef Uhrovčík ◽  
Monika Gyeváthová ◽  
Juraj Lesný
Keyword(s):  
Limit Of Detection ◽  
Molar Absorptivity ◽  
Model Solution ◽  
Arsenazo Iii ◽  
Complexing Agent ◽  
Acceptable Range ◽  
Relative Standard ◽  
Separation And Preconcentration ◽  
Interfering Ions

Abstract The concentration of Eu(III) cations in model aqueous solutions can be quantified by means of Arsenazo III reagent. Absorbance of the solution was measured at the wavelength λmax = 655 nm. Molar absorptivity reached the value ε655 = 5.5±0.2 · 104 cm-1 mol-1 · dm3. Beer's law was obeyed in the range from 0 to 2 mg · dm-3 Eu(III). The value of limit of detection was established by application of 3σ approach and reached the value of 20.9 μg · dm-3. Repeatability of analysis expressed by relative standard deviation does not exceed the value of ± 8% and apparent recovery lay in acceptable range from 91 to 106 %. Stoichiometry between Eu(III) and Arsenazo III in media of relevant solution was 1:1. The absorbance of the solutions within the linear range of the proposed method maintained a constant value for 60 minutes. Described procedure can be utilized to determination of Eu(III) concentration in real samples, but it is necessary eliminate interfering ions. Cations like La(III), Sm(III), Th(IV), U(VI) and complexing agent EDTA cause significant error at the determination of Eu(III) in model solution. Presented spectrophotometric method could be applied for the determination of europium in the minerals and water samples, however after a suitable separation and preconcentration of target analyte.

scholarly journals Analysis of Ethyl p-Methoxycinnamate from Kaempferia galanga L. Extract by High Performance Liquid Chromatography

2021 ◽  
Vol 5 (4) ◽  
pp. 353-358
Author(s):  
Wiwin Winingsih ◽  
Sri Gustini Husein ◽  
Rozalia Putri Neno Ramdhani
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Range Limit ◽  
Uv Detector ◽  
Kaempferia Galanga ◽  
System Suitability ◽  
Relative Standard ◽  
Limit Of Quantitation

Ethyl para-methoxycinamate (EPMS) is a major compound of Kaempferia galanga L that has anti-inflammatory effect.  The purpose of this study was to determine of EPMS in Kaempferiae galanga L rhizome extract by  High Performance Liquid Chromatography (HPLC) and evaluated the performance of the analysis. This study included determination of system suitability, accuracy, precision, linearity and range, limit of detection (LOD) and Limit of quantitation (LOQ) and selectivity.  The results of system suitability test  HPLC System for EPMS analysis were as follows isocratic elution system of a mobile phase mixture of methanol: water (70:30) containing 0.1% TFA, uv detector at a wavelength of 308 nm using column C18 (150 × 4, 6mm, 5μm) flow rate 1 ml / min. From the analysis, it was found that the average EPMS content was 78.74%. Then method had linear concentration range from 5-360 ppm, with R ² = 0.9999. The LOD and LOQ were 7.0722 ppm and 21.4311 ppm respectively. The accuracy of this method that represented by % recovery was 98.02% - 101.26%. The precision of this method that expressed by Relative Standard Deviation (RSD) was 1.57%. The selectivity of this method that showed by  resolution value was 2.6. Based on the results of the system suitability test and analysis performance evaluation,all parameters met the requirements.

A highly sensitive octopus-like azobenzene fluorescent probe for determination of abamectin B1 in apples

2020 ◽  
Author(s):  
Zhenlong Guo ◽  
YiFei Su ◽  
Kexin Li ◽  
MengYi Tang ◽  
Qiang Li ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Limit Of Detection ◽  
Quantitative Detection ◽  
Fluorescence Signal ◽  
Ionization Mass ◽  
Limit Of Quantification ◽  
Visible Absorption ◽  
Esi Ms ◽  
Relative Standard ◽  
Ft Ir

Abstract The development of detecting residual level of abamectin B1 in apples is of great importance to public health. Herein, we synthesized a octopus-like azobenzene fluorescent probe 1,3,5-tris (5'-[(E)-(p-phenoxyazo) diazenyl)] benzene-1,3-dicarboxylic acid) benzene (TPB) for preliminary detection of abamectin B1 in apples. The TPB molecule was characterized by ultraviolet-visible absorption spectrometry, 1H-nuclear magnetic resonance, fourier-transform infrared (FT-IR), electrospray ionization mass spectroscopy (ESI-MS) and fluorescent spectrum. A proper determination condition was optimized, with limit of detection and limit of quantification of 1.3 µg L-1 and 4.4 μg L-1, respectively. The mechanism of this probe to identify abamectin B1 was illustrated in terms of undergoing aromatic nucleophilic substitution, by comparing fluorescence changes, FT-IR and ESI-MS. Furthermore, a facile quantitative detection of the residual abamectin B1 in apples was achieved. Good reproducibility was shown based on relative standard deviation of 2.20%. Six carboxyl recognition sites, three azo groups and unique fluorescence signal towards abamectin B1 of this fluorescent probe decided ideal sensitivity, specificity and selectivity. The results show that the octopus-like azobenzene fluorescent probe may be promising for evaluating abamectin B1 in agricultural foods.

A simple flow injection spectrophotometric determination method for iron(III) based on O-acetylsalicylhydroxamic acid complexation

2009 ◽  
Vol 63 (3) ◽  
Author(s):  
Muberra Andac ◽  
Adem Asan ◽  
Ibrahim Isildak
Keyword(s):  
Standard Deviation ◽  
Flow Injection ◽  
Limit Of Detection ◽  
Total Iron ◽  
Relative Standard ◽  
Rapid Flow ◽  
Aas Method ◽  
Interfering Ions ◽  
Simple Flow

AbstractA simple and rapid flow-injection spectrophotometric method for the determination of iron(III) and total iron is proposed. The method is based on the reaction between iron(III) and O-acetylsalicylhydroxamic acid (AcSHA) in a 2 % methanol solution resulting in an intense violet complex with strong absorption at 475 nm. Optimum conditions for the determination of iron(III) and the interfering ions were tested. The relative standard deviation for the determination of 5 μg L−1 iron(III) was 0.85 % (n = 10), and the limit of detection (blank signal plus three times the standard deviation of the blank) was 0.5 μg L−1, both based on the injection volumes of 20 μL. The method was successfully applied in the determination of iron(III) and total iron in water and ore samples. The method was verified by analysing a certified reference material Zn/Al/Cu 43XZ3F and also by the AAS method.

Highly Sensitive Detection of miRNA-155 Using Molecular Beacon-Functionalized Monolayer MoS2 Nanosheet Probes with Duplex-Specific Nuclease-Mediated Signal Amplification

2021 ◽  
Vol 17 (6) ◽  
pp. 1034-1043
Author(s):  
Ying Liu ◽  
Zhou Ding ◽  
Jingjing Zhang ◽  
Chunyuan Song ◽  
Le Zhang ◽  
...  
Keyword(s):  
Human Serum ◽  
Fluorescent Probe ◽  
Molecular Beacon ◽  
Limit Of Detection ◽  
Fluorescence Signal ◽  
Linear Calibration ◽  
Highly Sensitive ◽  
Relative Standard ◽  
Highly Sensitive Detection ◽  
Fluorescence Amplification

MicroRNA-155 (miRNA-155) as a characteristic myeloma-associated biomarker exhibits significant potential application in the diagnosis of multiple myeloma (MM). In this paper, a novel type of molecular beacon (MB)-functionalized monolayer MoS2 nanosheet probe was proposed as fluorescent probe for high-sensitive assays of miRNA-155that uses a duplexspecificnuclease (DSN) enzyme to amplify the fluorescence signal. The preparation and detection conditions of the fluorescent probes were optimized in some aspects, such as the concentration of MoS2 (0.80 μM) and DSN (0.2 U), and the incubation time of DSN (30 min). The probesexhibited a sensitive fluorescence response to miRNA-155 and the fluorescence signal of the assay was significantly amplified by the cleavage of DSN. The relationship between F/F0 and logC miRNA follows a linear calibration curve, and the limit of detection (LOD) of miRNA-155 in 10% human serum is calculated to be 10.96 fM based on this relationship. The good performance and fluorescence amplification effect of the fluorescent probe were confirmed by studying the recovery of miRNA-155 in 10% human serum, which was ranged from 98.32% to 106.3% with a relative standard deviation of less than 4.14%. Besides, the high expression of miRNA-155 in clinic blood of MM patients was sensitively distinguished from healthy peoples by using the proposed probes. The proposed novel fluorescent probe based on the DSN can be used to detect miRNA-155 in human serum and provide a potential, convenient and reliable tool for diagnosis of MM.

scholarly journals Fluorescent Probe for Ag+ Detection Using SYBR GREEN I and C-C Mismatch

Biosensors ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 6
Author(s):  
Xiaohong Zhou ◽  
Abdul Ghaffar Memon ◽  
Weiming Sun ◽  
Fang Fang ◽  
Jinsong Guo
Keyword(s):  
Fluorescent Probe ◽  
Limit Of Detection ◽  
False Negative ◽  
Silver Ions ◽  
Bath Temperature ◽  
Repeated Measurements ◽  
Sybr Green ◽  
Sybr Green I ◽  
Relative Standard ◽  
Incubation Duration

Among heavy metals silver ions (Ag+) severely impact water, the environment and have serious side effects on human health. This article proposes a facile and ultrasensitive fluorescent probe for the detection of Ag+ ions using SYBR Green I (SGI) and cytosine-rich (C-rich) silver-specific oligonucleotide (SSO). Maximum fluorescent intensities with the highest sensitivity were obtained using a 0.61 dye/SSO base ratio (DBR). The established sensing principle using the optimized parameters for bath temperature, SSO concentration, DBR, ionic strength, pH, reaction time, incubation duration and temperature effect achieved a sensitive limit of detection of 59.9 nM for silver ions (calculated through 3σ, n = 11) with a linear working range of 100–1000 nM and 0.997 R2. The total time for one assay is below 10 min; The relative standard derivation for ten repeated measurements is 8.6%. No blatant interferences were observed in the selectivity test when fluorescent probe is evaluated by investigating the effects of 11 common interference factors in the aqueous matrix. In extreme cases, three false-negative factors were observed, including calcium hardness, magnesium hardness, and hypochlorite. The recovery ratios were within the range of 79~110% for three types of diluted water.

Calcium/Copper alginate framework doped with CuO nano-particles as a novel adsorbent for micro-extraction of benzodiazepines from human serum

2020 ◽  
Vol 16 ◽  
Author(s):  
Nadereh Rahbar ◽  
Fatemeh Ahmadi ◽  
Zahra Ramezani ◽  
Masoumeh Nourani
Keyword(s):  
Human Serum ◽  
High Performance ◽  
Limit Of Detection ◽  
Solid Phase ◽  
Nano Particles ◽  
Limit Of Quantification ◽  
Analytical Methodology ◽  
Fiber Fabrication ◽  
Relative Standard ◽  
Green Procedure

Background: Sample preparation is one of the most challenging phases in pharmaceutical analysis, especially in biological matrices, affecting the whole analytical methodology. Objective: In this study, a new Ca(II)/Cu(II)/alginate/CuO nanoparticles hydrogel fiber (CCACHF) was synthesized through a simple, green procedure and applied for fiber micro solid phase extraction (FMSPE) of diazepam (DIZ) and oxazepam (OXZ) as model drugs prior to high-performance liquid chromatography-UV detection (HPLC-UV). Methods: Composition and morphology of the prepared fiber were characterized and the effect of main parameters on the fiber fabrication and extraction efficiency have been studied and optimized. Results: In optimal conditions, calibration curves were linear ranging between 0.1–500 µg L−1 with regression coefficients of 0.9938 and 0.9968. Limit of detection (LOD) (S/N=3) and limit of quantification (LOQ) (S/N=10) of the technique for DIZ and OXZ were 0.03 to 0.1 µg L−1. Within-day and between-day relative standard deviations (RSDs) for DIZ and OXZ were 6.0–12.5% and 3.3–9.4%, respectively. Conclusion: The fabricated adsorbent has been substantially employed to extraction of selected benzo-diazepines (BZDs) from human serum real specimens and the obtained recoveries were also satisfactory (82.1-109.7%).

Green Synthesis and Spectroscopic Studies of Ag-rGO Nanocomposites for Highly Selective Mercury (II) Sensing

2018 ◽  
Vol 9 (1) ◽  
pp. 101-108 ◽  
Author(s):  
Shubhangi J. Mane-Gavade ◽  
Sandip R. Sabale ◽  
Xiao-Ying Yu ◽  
Gurunath H. Nikam ◽  
Bhaskar V. Tamhankar
Keyword(s):  
Green Synthesis ◽  
Color Change ◽  
Limit Of Detection ◽  
Aqueous Media ◽  
Suitable Condition ◽  
Cost Effective ◽  
Spectroscopic Studies ◽  
Calibration Plot ◽  
First Time

Introduction: Herein we report the green synthesis and characterization of silverreduced graphene oxide nanocomposites (Ag-rGO) using Acacia nilotica gum for the first time. Experimental: We demonstrate the Hg2+ ions sensing ability of the Ag-rGO nanocomposites form aqueous medium. The developed colorimetric sensor method is simple, fast and selective for the detection of Hg2+ ions in aqueous media in presence of other associated ions. A significant color change was noticed with naked eye upon Hg2+ addition. The color change was not observed for cations including Sr2+, Ni2+, Cd2+, Pb2+, Mg2+, Ca2+, Fe2+, Ba2+ and Mn2+indicating that only Hg2+ shows a strong interaction with Ag-rGO nanocomposites. Under the most suitable condition, the calibration plot (A0-A) against concentration of Hg2+ was linear in the range of 0.1-1.0 ppm with a correlation coefficient (R2) value 0.9998. Results & Conclusion The concentration of Hg2+ was quantitatively determined with the Limit of Detection (LOD) of 0.85 ppm. Also, this method shows excellent selectivity towards Hg2+ over nine other cations tested. Moreover, the method offers a new cost effective, rapid and simple approach for the detection of Hg2+ in water samples.

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