scholarly journals Acid Lipase fromCandida viswanathii: Production, Biochemical Properties, and Potential Application

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Alex Fernando de Almeida ◽  
Sâmia Maria Tauk-Tornisielo ◽  
Eleonora Cano Carmona
Keyword(s):  
Potential Application ◽  
Soybean Lecithin ◽  
Lipase Production ◽  
Biochemical Properties ◽  
Lipolytic Enzyme ◽  
Acid Lipase ◽  
Food Industries ◽  
Wide Range ◽  
Fermentation Parameters ◽  
Hydrolysis Of

Influences of environmental variables and emulsifiers on lipase production of aCandida viswanathiistrain were investigated. The highest lipase activity (101.1 U) was observed at 210 rpm, pH 6.0, and 27.5°C. Other fermentation parameters analyzed showed considerable rates of biomass yield ( g/h). Addition of soybean lecithin increased lipase production in 1.45-fold, presenting lipase yield () of 10.061 U/g. Crude lipase presented optimal activity at acid pH of 3.5, suggesting a new lipolytic enzyme for this genus and yeast in general. In addition, crude lipase presented high stability in acid conditions and temperature between 40 and 45°C, after 24 h of incubation in these temperatures. Lipase remained active in the presence of organic solvents maintaining above 80% activity in DMSO, methanol, acetonitrile, ethanol, acetone, 1-propanol, isopropanol, and 2-propanol. Effectiveness for the hydrolysis of a wide range of natural triglycerides suggests that this new acid lipase has high potential application in the oleochemical and food industries for hydrolysis and/or modification of triacylglycerols to improve the nutritional properties.

PROBIOTIC PROPERTIES AND RELEVANCE OF PRACTICAL APPLICATION L. ACIDOPHILUS

2021 ◽  
pp. 70-75
Author(s):  
I. V. Boyarineva
Keyword(s):  
Lactobacillus Acidophilus ◽  
Biochemical Properties ◽  
Starter Cultures ◽  
Active Principle ◽  
Probiotic Properties ◽  
Food Industries ◽  
Urgent Task ◽  
Wide Range ◽  
Traditional Dairy Products ◽  
Probiotic Activity

Creating probiotic complex starter cultures and increasing the probiotic activity of traditional dairy products is an urgent task today. The active principle of these products is live lactobacilli, in particular, L. Acidophilus, which have a wide range of probiotic activity. The article is devoted to the description of the probiotic culture of Lactobacillus acidophilus. The article describes the probiotic and biochemical properties of Lactobacillus Acidophilus, provides examples of the practical use of this culture in the medical and food industries.

scholarly journals Enzymatic Methods for the Manipulation and Valorization of Soapstock from Vegetable Oil Refining Processes

2021 ◽  
Vol 2 (1) ◽  
pp. 74-91
Author(s):  
Beatrice Casali ◽  
Elisabetta Brenna ◽  
Fabio Parmeggiani ◽  
Davide Tessaro ◽  
Francesca Tentori
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Vegetable Oil ◽  
Lipase Production ◽  
Oil Refining ◽  
Flow Mode ◽  
Acid Oil ◽  
Industrial Level ◽  
Vegetable Oil Refining ◽  
Hydrolysis Of

The review will discuss the methods that have been optimized so far for the enzymatic hydrolysis of soapstock into enriched mixtures of free fatty acids, in order to offer a sustainable alternative to the procedure which is currently employed at the industrial level for converting soapstock into the by-product known as acid oil (or olein, i.e., free fatty acids removed from raw vegetable oil, dissolved in residual triglycerides). The further biocatalyzed manipulation of soapstock or of the corresponding acid oil for the production of biodiesel and fine chemicals (surfactants, plasticizers, and additives) will be described, with specific attention given to processes performed in continuous flow mode. The valorization of soapstock as carbon source in industrial lipase production will be also considered.

scholarly journals Phosphatase synthesis in Klebsiella (Aerobacter) aerogenes growing in continuous culture

1972 ◽  
Vol 127 (1) ◽  
pp. 87-96 ◽  
Author(s):  
P. G. Bolton ◽  
A. C. R. Dean
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Continuous Culture ◽  
Activity Profile ◽  
Glucose Effect ◽  
Ph Values ◽  
Nitrophenyl Phosphate ◽  
Wide Range ◽  
Rate Of Hydrolysis ◽  
Hydrolysis Of

1. Phosphatase synthesis was studied in Klebsiella aerogenes grown in a wide range of continuous-culture systems. 2. Maximum acid phosphatase synthesis was associated with nutrient-limited, particularly carbohydrate-limited, growth at a relatively low rate, glucose-limited cells exhibiting the highest activity. Compared with glucose as the carbon-limiting growth material, other sugars not only altered the activity but also changed the pH–activity profile of the enzyme(s). 3. The affinity of the acid phosphatase in glucose-limited cells towards p-nitrophenyl phosphate (Km 0.25–0.43mm) was similar to that of staphylococcal acid phosphatase but was ten times greater than that of the Escherichia coli enzyme. 4. PO43−-limitation derepressed alkaline phosphatase synthesis but the amounts of activity were largely independent of the carbon source used for growth. 5. The enzymes were further differentiated by the effect of adding inhibitors (F−, PO43−) and sugars to the reaction mixture during the assays. In particular, it was shown that adding glucose, but not other sugars, stimulated the rate of hydrolysis of p-nitrophenyl phosphate by the acid phosphatase in carbohydrate-limited cells at low pH values (<4.6) but inhibited it at high pH values (>4.6). Alkaline phosphatase activity was unaffected. 6. The function of phosphatases in general is discussed and possible mechanisms for the glucose effect are outlined.

scholarly journals Camel Gelatin Composition, Properties, Production, and Applications

2020 ◽  
pp. 306-327
Author(s):  
Irwandi Jaswir ◽  
Hassan Abdullah Al-Kahtani ◽  
Fitri Octavianti ◽  
Widya Lestari ◽  
Nurlina Yusof
Keyword(s):  
Body Temperature ◽  
High Water ◽  
Partial Hydrolysis ◽  
High Water Content ◽  
Food Ingredient ◽  
Normal Body Temperature ◽  
Food Industries ◽  
Reversible Gel ◽  
Composition Properties ◽  
Hydrolysis Of

Gelatin is an important protein produced through partial hydrolysis of collagen from animal parts and byproducts such as cartilage, bones, tendons, and hides. The ability of gelatin to form a thermo-reversible gel at normal body temperature and high water content make it an exceptional food ingredient. A good quality gelatin is translucent, brittle, colorless (sometimes slightly yellow), bland in taste, and odorless. Gelatin has been found useful as stabilizer and filler in dairy products and other food industries. Recently, the global gelatin production net over 300,000 metric tons: 46% were from pigskin, 29.4% from bovine hides, 23.1% from bones, and 1.5% from other parts. Although camels have been recognized as source of meat and milk, utilization of camel bones and skins for gelatin production has not been fully explored. This chapter will discuss the processing of camel gelatin extraction.

scholarly journals Bioprocesses with Reduced Ecological Footprint by Marine Debaryomyces hansenii Strain for Potential Applications in Circular Economy

2021 ◽  
Vol 7 (12) ◽  
pp. 1028
Author(s):  
Silvia Donzella ◽  
Claudia Capusoni ◽  
Luisa Pellegrino ◽  
Concetta Compagno
Keyword(s):  
Ecological Footprint ◽  
Debaryomyces Hansenii ◽  
High Growth ◽  
High Growth Rate ◽  
Food Industries ◽  
Starting Point ◽  
Wide Range ◽  
Potential Applications ◽  
A Cell ◽  
Marine Strain

The possibility to perform bioprocesses with reduced ecological footprint to produce natural compounds and catalyzers of industrial interest is pushing the research for salt tolerant microorganisms able to grow on seawater-based media and able to use a wide range of nutrients coming from waste. In this study we focused our attention on a Debaryomyces hansenii marine strain (Mo40). We optimized cultivation in a bioreactor at low pH on seawater-based media containing a mixture of sugars (glucose and xylose) and urea. Under these conditions the strain exhibited high growth rate and biomass yield. In addition, we characterized potential applications of this yeast biomass in food/feed industry. We show that Mo40 can produce a biomass containing 45% proteins and 20% lipids. This strain is also able to degrade phytic acid by a cell-bound phytase activity. These features represent an appealing starting point for obtaining D. hansenii biomass in a cheap and environmentally friendly way, and for potential use as an additive or to replace unsustainable ingredients in the feed or food industries, as this species is included in the QPS EFSA list (Quality Presumption as Safe—European Food Safety Authority).

scholarly journals Proanthocyanidins: Oligomeric Structures with Unique Biochemical Properties and Great Therapeutic Promise

2012 ◽  
Vol 7 (3) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Zhanjie Xu ◽  
Peng Du ◽  
Peter Meiser ◽  
Claus Jacob
Keyword(s):  
Protein Interactions ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Chemical Properties ◽  
Cancer Chemoprevention ◽  
Biochemical Properties ◽  
Synthetic Methods ◽  
Practical Applications ◽  
Wide Range ◽  
Physical And Chemical

Proanthocyanidins represent a unique class of oligomeric and polymeric secondary metabolites found ubiquitously and in considerable amounts in plants and some algae. These substances exhibit a range of rather surprising physical and chemical properties which, once applied to living organisms, are translated into a multitude of biological activities. The latter include antioxidant properties, cancer chemoprevention, anti-inflammatory and anti-diabetic effects as well as some exceptional, yet highly interesting activities, such as anti-nutritional and antimicrobial activity. Despite the wide range of activities and possible medical/agricultural applications of proanthocyanidins, many questions still remain, including issues related to bioavailability, metabolism and the precise biochemical, extra- and intracellular targets and mode(s) of action of these highly potent materials. Among the various physical and chemical interactions of such substances, strong binding to proteins appears to form the basis of many of their biological activities. Once easy-to-use synthetic methods to produce appropriate quantities of pure proanthocyanidins are available, it will be possible to identify the prime biological targets of these oligomers, study oligomer-protein interactions in more detail and develop possible practical applications in medicine and agriculture.

Biophysical and functional characterizations of recombinant RimI acetyltransferase from Mycobacterium tuberculosis

2019 ◽  
Vol 51 (9) ◽  
pp. 960-968
Author(s):  
Meijing Hou ◽  
Jie Zhuang ◽  
Shihui Fan ◽  
Huilin Wang ◽  
Chenyun Guo ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Quantum Coherence ◽  
Protein Modification ◽  
Structural Information ◽  
Biochemical Properties ◽  
Structural Determination ◽  
Physiological Processes ◽  
High Quality Protein ◽  
Wide Range ◽  
Docking Approach

Abstract Nα-acetylation is a universal protein modification related to a wide range of physiological processes in eukaryotes and prokaryotes. RimI, an Nα-acetyltransferase in Mycobacterium tuberculosis, is responsible for the acetylation of the α-amino group of the N-terminal residue in the ribosomal protein S18. Despite growing evidence that protein acetylation may be correlated with the pathogenesis of tuberculosis, no structural information is yet available for mechanistically understanding the MtRimI acetylation. To enable structural studies for MtRimI, we constructed a serial of recombinant MtRimI proteins and assessed their biochemical properties. We then chose an optimal construct MtRimIC21A4-153 and expressed and purified the truncated high-quality protein for further biophysical and functional characterizations. The 2D 1H-15N heteronuclear single quantum coherence spectrum of MtRimIC21A4-153 exhibits wider chemical shift dispersion and favorable peak isolation, indicating that MtRimIC21A4-153 is amendable for further structural determination. Moreover, bio-layer interferometry experiments showed that MtRimIC21A4-153 possessed similar micromolar affinity to full-length MtRimI for binding the hexapeptide substrate Ala-Arg-Tyr-Phe-Arg-Arg. Enzyme kinetic assays also exhibited that MtRimIC21A4-153 had almost identical enzymatic activity to MtRimI, indicating insignificant influence of the recombinant variations on enzymatic functions. Furthermore, binding sites of the peptide were predicted by molecular docking approach, suggesting that this substrate binds to MtRimI primarily through electrostatic and hydrogen bonding interactions. Our results lay a foundation for the further structural determination and dynamics detection of MtRimI.

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