Characterization ofMycobacterium bovisfrom Humans and Cattle in Namwala District, Zambia
Tuberculosis remains a major public health problem in Zambia. While human to human transmission ofMycobacterium tuberculosisis of major importance in driving the tuberculosis epidemic, the impact ofMycobacterium bovistransmission from infected cattle is largely unknown. This cross-sectional study aimed at molecular characterization ofM. bovisin humans and cattle. A total of 100 human sputum samples and 67 bovine tissues were collected and analyzed for the presence of mycobacteria. Of 65 human samples that harbored acid fast bacteria (AFB), 55 isolates were obtained of which 34 were identified asM. tuberculosisand 2 asM. bovis. AFB-positive bovine samples (n=67) yielded 47 mycobacterial isolates among which 25 were identified asM. bovisand noM. tuberculosiswas found. Among theM. bovisisolates, spoligotyping revealed a high homogeneity in genotypes circulating in Namwala district. Human and cattle isolates shared identical MIRU-VNTR genotypes, suggesting that transmission between the two hosts may occur. Therefore, this study has documented zoonotic TB in human patients in Namwala district of Zambia. However, further molecular epidemiological studies in the study area are recommended.