scholarly journals Probiotic Potential ofLactobacillusStrains with Antimicrobial Activity against Some Human Pathogenic Strains

2014 ◽  
Vol 2014 ◽  
pp. 1-16 ◽  
Author(s):  
Parisa Shokryazdan ◽  
Chin Chin Sieo ◽  
Ramasamy Kalavathy ◽  
Juan Boo Liang ◽  
Noorjahan Banu Alitheen ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Activities ◽  
Rrna Gene ◽  
Human Pathogens ◽  
Bacterial Strains ◽  
Probiotic Potential ◽  
Good Ability ◽  
Acid And Bile Tolerance ◽  
Infant Feces

The objective of this study was to isolate, identify, and characterize some lactic acid bacterial strains from human milk, infant feces, and fermented grapes and dates, as potential probiotics with antimicrobial activity against some human pathogenic strains. One hundred and forty bacterial strains were isolated and, after initial identification and a preliminary screening for acid and bile tolerance, nine of the best isolates were selected and further identified using 16 S rRNA gene sequences. The nine selected isolates were then characterizedin vitrofor their probiotic characteristics and their antimicrobial activities against some human pathogens. Results showed that all nine isolates belonged to the genusLactobacillus. They were able to tolerate pH 3 for 3 h, 0.3% bile salts for 4 h, and 1.9 mg/mL pancreatic enzymes for 3 h. They exhibited good ability to attach to intestinal epithelial cells and were not resistant to the tested antibiotics. They also showed good antimicrobial activities against the tested pathogenic strains of humans, and most of them exhibited stronger antimicrobial activity than the reference strainL. caseiShirota. Thus, the nineLactobacillusstrains could be considered as potential antimicrobial probiotic strains against human pathogens and should be further studied for their human health benefits.

Heteroaromatization with 4-Phenyldiazenyl-1-naphthol. Part IV: Synthesis of Some New Heterocyclic Compounds with Potential Biological Activity

2019 ◽  
Vol 16 (6) ◽  
pp. 931-938
Author(s):  
Hany M. Mohamed ◽  
Ashraf H.F. Abd El-Wahab
Keyword(s):  
Antimicrobial Activity ◽  
Agar Medium ◽  
Potassium Hydroxide Solution ◽  
Antimicrobial Activities ◽  
Azo Compounds ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Pharmacological Agents ◽  
Tetrazole Derivatives

Background: Synthetic azo compounds and their derivatives have been studied extensively due to their biological and pharmacological activities. Pyranopyridines, pyranopyrimidines and tetrazoles derivatives have emerged as a promising and attractive scaffold in the development of potent biological and pharmacological agents. Objectives: To design a series of new benzochromeno(pyridine/pyrimidine/tetrazole) derivatives and evaluate their antimicrobial activity against some bacterial strains (Gram-positive and Gram-negative) and some fungal strains. Materials and Methods: The (E)-7-(4-chlorophenyl)-5-(phenyldiazenyl)-10-thioxo-7,9,10,11-tetrahydro-8Hnaptho[ 1,2-b]pyrano[2,3-d]pyrimidin-8-one (4) was synthesized by the reaction of 4H-naphtho[1,2-b]pyran-3- carbonitrile (3) with carbon disulfide in alcoholic potassium hydroxide solution. Reaction of 3 with sodium azide in DMF and in presence of ammonium chloride afforded 6-(phenyldiazenyl)-3-(1H-tetrazol-5-yl)-4Hbenzo[ h]chromen-2-amine (7) while with malononitrile, thiourea or urea gave chromeno[2,3-b]pyridine-9- carbonitrile (8), chromeno[2,3-d]pyrimidine-10-thione (9) and chromeno[2,3-d]pyrimidin-10-one (10), respectively. The assignment structures were established on the basis of spectral data. Results: In this study, the antimicrobial activity of the synthesized compounds 3-12 was examined for their in vitro antimicrobial activity by using agar diffusion method such as Mueller-Hinton agar medium for bacteria and Sabouraud’s agar medium for fungi. Ampicillin and mycostatine were included in the experiments as reference drugs. Conclusion: A series of new benzochromeno(pyridine/pyrimidine/tetrazole) derivatives were synthesized in this work. All compounds were evaluated in antimicrobial activities.

scholarly journals Design, Synthesis, and Evaluation of Novel 3-, 4-substituted, and 3,4-di Substituted Quinazoline Derivatives as Antimicrobial Agents

2020 ◽  
Vol 71 (2) ◽  
pp. 422-435
Author(s):  
Farag A. El-Essawy ◽  
Abdulrahman I. Alharthi ◽  
Mshari A. Alotaibi ◽  
Nancy E. Wahba ◽  
Nader M. Boshta
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agents ◽  
Antimicrobial Activities ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Design Synthesis ◽  
Gram Negative ◽  
Spectroscopic Analyses ◽  
Quinazoline Derivatives

A novel series of 3-, 4-substituted, and 3,4-di substituted quinazoline derivatives were prepared via various cyclized regents and most of the newly prepared compounds evaluated for their antimicrobial activities in vitro against Gram-positive, Gram-negative bacterial strains and fungi strains. The structures of the quinazoline derivatives have been confirmed using spectroscopic analyses (IR, NMR, and EI-MS). Some of the synthesized derivatives displayed a moderate antimicrobial activity in comparison with reference drugs, for example compounds 13d, 15a, 17b, 18b, 18d, 25, and 29a-c. Among the synthesized compounds, the pyrimidoqunazoline derivative 6c elicited the highest activity.

IN VITRO EVALUATION OF ANTIMICROBIAL ACTIVITY AND PROBIOTIC POTENTIAL OF LACTOBACILLUS STRAINS AGAINST SOME HUMAN PATHOGENS

2018 ◽  
pp. 31-35
Keyword(s):  
Antimicrobial Activity ◽  
Bacterial Species ◽  
Acid Tolerance ◽  
Lactobacillus Species ◽  
Human Pathogens ◽  
Probiotic Potential ◽  
Probiotic Strains ◽  
Potential Methods ◽  
Lactobacillus Spp

Objective: Curd and yogurt are the major source of probiotic consumed by majority of the Indian population and Lactobacillus spp. are the predominant bacterial species in them. The present study was carried out to isolate Lactobacillus spp, from locally available dairy products and assess for the probiotic potential. Methods: The isolated were identified using biochemical assays and assessed for the properties such as Acid tolerance, Bile tolerance and NaCl tolerance. The identified strains were also subjected to antibiotic susceptibility and Antimicrobial activity using standard methodology. Results: A total of ten Lactic acid bacteria were isolated form different sources, belonging to different Lactobacillus species viz. L. acidophilus IAMb-3, L. brevis IAMb-6, L. bulgaricus IAMb-9, L. lactis IAMb-10, L. acidophilus IAMb-11, L. fermentum IAMb-13, L. casei IAMb-14, L. casei IAMb-15, L. plantarum IAMb-16 and L. rhamonsus IAMb-18. These identified strains were able to tolerate pH-3, 0.3% bile salt and upto 5-6% NaCl. Seven out of ten stains had antimicrobial activity against the pathogenic strain used in the study. Conclusion: In the present study the some of the potential probiotic strains have been isolated from local commercial and homemade sources. The isolated strains were capable of tolerating the harsh similar to human gut environment and found antagonistic to gut pathogens, hence can considered as potential probiotics.

Exploitation of the Antioxidant Potential of Geranium Macrorrhizum (Geraniaceae): Hepatoprotective and Antimicrobial Activities

2012 ◽  
Vol 7 (12) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Niko S. Radulović ◽  
Milan B. Stojković ◽  
Snežana S. Mitić ◽  
Pavle J. Randjelović ◽  
Ivan R. Ilić ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Plant Species ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Antioxidant Potential ◽  
Human Pathogens ◽  
Methanol Extracts

In this study we evaluated in vitro (radical scavenging) and in vivo (hepatoprotective effect) antioxidant activities and antimicrobial properties of the extracts of the above- and underground parts of Geranium macrorrhizum L. (Geraniaceae), an ethnopharmacologically renowned plant species. The antioxidant activity and total phenol and flavonoid contents of four different solvent extracts were evaluated by seven different methods. The methanol extracts, administered i.p. to rats (120-480 mg/kg), were evaluated for hepatoprotective activity in a CCl4-induced hepatotoxicity model. The same extracts were tested for antimicrobial activity against seven bacterial and two fungal species. The administered methanol extracts with the highest antioxidant potential showed a significant dose-dependent hepatoprotective action against CCl4-induced liver damage in both decreasing the levels of liver transaminases and bilirubin and in reducing the extent of morphological malformations of the liver. The leaf methanol extract displayed a very strong antibacterial activity, especially against Staphylococcus aureus, with low minimal inhibitory and bactericidal concentrations. These results justify the frequent use of this plant species in folk medicine. Besides the known astringent effect, one can expect that the observed antimicrobial activity against several human pathogens contributes to the wound healing properties of this plant.

scholarly journals Synthesis of Oxadiazolyl, Pyrazolyl and Thiazolyl Derivatives of Thiophene-2-Carboxamide as Antimicrobial and Anti-HCV Agents

2017 ◽  
Vol 11 (1) ◽  
pp. 38-53 ◽  
Author(s):  
Ola H. Rizk ◽  
Omaima G. Shaaban ◽  
Abeer E. Abdel Wahab
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Activities ◽  
Hcv Rna ◽  
Bacterial Strains ◽  
Low Concentration ◽  
Oxadiazole Derivatives ◽  
Polymerase Chain ◽  
New Compounds ◽  
Derivatives Of

Introduction: Three series of pyrazole, thiazole and 1,3,4-oxadiazole, derivatives were synthesized starting from 5-amino-4-(hydrazinocarbonyl)-3-methylthiophene-2-carboxamide (2). Methods: All compounds were investigated for their preliminary antimicrobial activity. They were proved to exhibit remarkable antimicrobial activity against Pseudomonas aeruginosa with insignificant activity towards Gram positive bacterial strains and fungi. Results: In-vitro testing of the new compounds on hepatitis-C virus (HCV) replication in hepatocellular carcinoma cell line HepG2 infected with the virus utilizing the reverse transcription polymerase chain reaction technique (RT-PCR) generally showed inhibition of the replication of HCV RNA (–) strands at low concentration, while, eight compounds; 3a, 6, 7a, 7b, 9a, 9b, 10a and 11b proved to inhibit the replication of HCV RNA (+) and (–) strands at very low concentration range 0.08-0.36 μg/mL. Conclusion: Compounds 7b and 11b displayed the highest anti-HCV and antimicrobial activities in this study.

scholarly journals Evaluation of Antimicrobial Activity of Extracts of Terminalia chebula and Piper nigrum against Streptococcus mutans: An In Vitro Study

2021 ◽  
pp. 218-224
Author(s):  
Pavani Bellamkonda ◽  
Ramesh Kumar Koothati ◽  
Aamina Bee ◽  
Abhishree Desai ◽  
G. K. Aarthi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibiotic Resistance ◽  
In Vitro Study ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Piper Nigrum ◽  
Terminalia Chebula ◽  
Bacterial Strains ◽  
Antimicrobial Potency

Objective: Dental caries is an infectious disease in which S. mutans plays a key role. Haphazard and irrational use of antibiotics leads to antibiotic resistance and fatal diarrhoeal diseases in children. Antimicrobial potency of Terminalia chebula and Piper nigrum extracts against several bacterial strains have been documented. The aims of this study were to assess and compare the antimicrobial activities of T. chebula and P. nigrum extracts against S. mutans with Ciprofloxacin as the positive control. Materials and Methods: For this purpose, S. mutans was isolated from plaque samples of people with active caries lesions.  Antimicrobial potency of both T. chebula and P.nigrum were tested using agar well diffusion method. Results: All the tested extracts showed antibacterial activity against S. mutans bacteria. Regarding the two tested herbs extracts, a higher antimicrobial activity was shown by the methanol extract of T. chebula with a mean diameter of inhibition zone was 26.75mm and a minimum inhibitory concentration (MIC) at 25mg /ml concentration followed by acetonic extract. Conclusion: These findings confirm the Antimicrobial potency of T. chebula which can be used as an alternative antibiotic and/or in combination with allopathic antibiotics to prevent the antibiotic resistance.

scholarly journals In vitro study of antimicrobial activity of some plant seeds against bacterial strains causing food poisoning diseases

2022 ◽  
Vol 82 ◽  
Author(s):  
A. A. Abu-Zaid ◽  
A. Al-Barty ◽  
K. Morsy ◽  
H. Hamdi
Keyword(s):  
Antimicrobial Activity ◽  
Nigella Sativa ◽  
Food Poisoning ◽  
In Vitro Study ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Bacterial Strains ◽  
Plant Seeds

Abstract In this research, some plant seeds powder was evaluated to find their potential effect to rule diseases of food poisoning. Antimicrobial effect of five plant seeds was examined contra Bacillus cereus, Staphylococcus aureus, Escherichia coli, Klebsiella. pneumonia and Candida albicans by using well diffusion method. Antimicrobial activity studies revealed high potential activity of plant seeds powder of Nigella sativa L., cucurbita pepo, Sesamum radiatum, Trigonella foenum-graecum, Linum usitatissimum with variable efficiency contra tested microbial strains with concentration of 100 mg/ml, except Sesamum radiatum scored no effect. The T. foenum and N. sativa seed powder showed the largest inhibition zone (24-20 mm) contra K. pneumonia, followed by S. aureus (20-18 mm) and C. albicans (15mm) respectively. The five plant seeds powder exhibited bacteriostatic and bactericidal effects with MIC’s 20 and MBC 40 mg/ml against K. pneumonia, and MIC’s 40 and MBC 60 mg/ml against S. aureus. The results of this study indicated that plants seeds powder have promising antimicrobial activities and their potential applications in food process. It could be utilized as a natural medicinal alternative instead of chemical substance.

scholarly journals In Vitro Antimicrobial Activities of Organic Acids and Their Derivatives on Several Species of Gram-Negative and Gram-Positive Bacteria

Molecules ◽  
2019 ◽  
Vol 24 (20) ◽  
pp. 3770 ◽  
Author(s):  
Lauren Kovanda ◽  
Wen Zhang ◽  
Xiaohong Wei ◽  
Jia Luo ◽  
Xixi Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Streptococcus Pneumoniae ◽  
Organic Acids ◽  
Enterococcus Faecalis ◽  
Sodium Formate ◽  
Antimicrobial Activities ◽  
Bacterial Strains ◽  
Gram Negative

The objective of this study was to determine the in vitro antimicrobial activity of several organic acids and their derivatives against Gram-positive (G+) and Gram-negative (G−) bacteria. Butyric acid, valeric acid, monopropionin, monobutyrin, monovalerin, monolaurin, sodium formate, and ProPhorce—a mixture of sodium formate and formic acid (40:60 w/v)—were tested at 8 to 16 concentrations from 10 to 50,000 mg/L. The tested bacteria included G− bacteria (Escherichia coli, Salmonella enterica Typhimurium, and Campylobacter jejuni) and G+ bacteria (Enterococcus faecalis, Clostridium perfringens, Streptococcus pneumoniae, and Streptococcus suis). Antimicrobial activity was expressed as minimum inhibitory concentration (MIC) of tested compounds that prevented growth of tested bacteria in treated culture broth. The MICs of butyric acid, valeric acid, and ProPhorce varied among bacterial strains with the lowest MIC of 500–1000 mg/L on two strains of Campylobacter. Sodium formate at highest tested concentrations (20,000 mg/L) did not inhibit the growth of Escherichia coli, Salmonella Typhimurium, and Enterococcus faecalis, but sodium formate inhibited the growth of other tested bacteria with MIC values from 2000 to 18,800 mg/L. The MIC values of monovalerin, monolaurin, and monobutyrin ranged from 2500 to 15,000 mg/L in the majority of bacterial strains. Monopropionin did not inhibit the growth of all tested bacteria, with the exception that the MIC of monopropionin was 11,300 mg/L on Clostridia perfringens. Monolaurin strongly inhibited G+ bacteria, with the MIC value of 10 mg/L against Streptococcus pneumoniae. The MIC tests indicated that organic acids and their derivatives exhibit promising antimicrobial effects in vitro against G− and G+ bacteria that are resistant to antimicrobial drugs. The acid forms had stronger in vitro antimicrobial activities than ester forms, except that the medium chain fatty acid ester monolaurin exhibited strong inhibitory effects on G+ bacteria.

scholarly journals Synthesis, lipophilicity and antimicrobial activity evaluation of some new thiazolyl-oxadiazolines

2015 ◽  
Vol 88 (4) ◽  
pp. 521-529 ◽  
Author(s):  
Cristina Ioana Stoica ◽  
Ioana Ionuț ◽  
Adrian Pîrnău ◽  
Carmen Pop ◽  
Ancuța Rotar ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agents ◽  
Chemical Characterization ◽  
Principal Component ◽  
Antimicrobial Activities ◽  
Bacterial Strains ◽  
Pca Method ◽  
Physical And Chemical ◽  
Layer Chromatography

Background and aims. Synthesis of new potential antimicrobial agents and evaluation of their lipophilicity.Methods. Ten new thiazolyl-oxadiazoline derivatives were synthesized and their structures were validated by 1H-NMR and mass spectrometry. The lipophilicity of the compounds was evaluated using the principal component analysis (PCA) method. The necessary data for applying this method were obtained by reverse-phase thin-layer chromatography (RP-TLC). The antimicrobial activities were tested in vitro against four bacterial strains and one fungal strain.Results. The lipophilicity varied with the structure but could not be correlated with the antimicrobial activity, since this was modest.Conclusions. We have synthesized ten new heterocyclic compounds. After their physical and chemical characterization, we determined their lipophilicity and screened their antimicrobial activity.

scholarly journals Antibacterial Activity of Bees Gut Lactobacilli against Paenibacillus Larvae In Vitro

2018 ◽  
Vol 2 (1) ◽  
pp. 7-10 ◽  
Author(s):  
Miroslava Kačániová ◽  
Jaroslav Gasper ◽  
Margarita Terentjeva ◽  
Simona Kunová ◽  
Maciej Kluz ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antimicrobial Activities ◽  
Paenibacillus Larvae ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
De Man ◽  
Lactobacillus Spp

Abstract The aim of this study was to evaluate antimicrobial activity of bees gastrointestinal Lactobacillus spp. of against Paenibacillus larvae. Content of the intestinal tract was cultured for isolation of Lactobacillus spp. Gut homogenates were plated on de Man, Rogosa and Sharpe agar (MRS, Oxoid) plates and incubated for 48-72h at 30°C anaerobically. Then, the identification of isolates with MALDI-TOF MS Biotyper was done. The bacterial strains Lactobacillus gasseri, L. amylovorus, L. kunkeei, L. fructivorans, Paenibacillus larvae were isolated from gut content of bees. The disc diffusion method was used for the determination of antimicrobial activities of the Lactobacillus supernatant against two strains of Paenibacillus larvae. The best antimicrobial activity of Lactobacillus against Paenibacillus larvae from gut was found in L. gasseri supernatant. Lesser degree of antimicrobial activity against P. larvae was found in L. kunkeei supernatant. The strongest antibacterial activity against P. larvae CCM 4438 was found in L. gasseri and L. amylovorus and the least antibacterial activity was found in L. fructivorans.

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