scholarly journals In Vitro Antimicrobial Activities of Organic Acids and Their Derivatives on Several Species of Gram-Negative and Gram-Positive Bacteria

Molecules ◽  
2019 ◽  
Vol 24 (20) ◽  
pp. 3770 ◽  
Author(s):  
Lauren Kovanda ◽  
Wen Zhang ◽  
Xiaohong Wei ◽  
Jia Luo ◽  
Xixi Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Streptococcus Pneumoniae ◽  
Organic Acids ◽  
Enterococcus Faecalis ◽  
Sodium Formate ◽  
Antimicrobial Activities ◽  
Bacterial Strains ◽  
Gram Negative

The objective of this study was to determine the in vitro antimicrobial activity of several organic acids and their derivatives against Gram-positive (G+) and Gram-negative (G−) bacteria. Butyric acid, valeric acid, monopropionin, monobutyrin, monovalerin, monolaurin, sodium formate, and ProPhorce—a mixture of sodium formate and formic acid (40:60 w/v)—were tested at 8 to 16 concentrations from 10 to 50,000 mg/L. The tested bacteria included G− bacteria (Escherichia coli, Salmonella enterica Typhimurium, and Campylobacter jejuni) and G+ bacteria (Enterococcus faecalis, Clostridium perfringens, Streptococcus pneumoniae, and Streptococcus suis). Antimicrobial activity was expressed as minimum inhibitory concentration (MIC) of tested compounds that prevented growth of tested bacteria in treated culture broth. The MICs of butyric acid, valeric acid, and ProPhorce varied among bacterial strains with the lowest MIC of 500–1000 mg/L on two strains of Campylobacter. Sodium formate at highest tested concentrations (20,000 mg/L) did not inhibit the growth of Escherichia coli, Salmonella Typhimurium, and Enterococcus faecalis, but sodium formate inhibited the growth of other tested bacteria with MIC values from 2000 to 18,800 mg/L. The MIC values of monovalerin, monolaurin, and monobutyrin ranged from 2500 to 15,000 mg/L in the majority of bacterial strains. Monopropionin did not inhibit the growth of all tested bacteria, with the exception that the MIC of monopropionin was 11,300 mg/L on Clostridia perfringens. Monolaurin strongly inhibited G+ bacteria, with the MIC value of 10 mg/L against Streptococcus pneumoniae. The MIC tests indicated that organic acids and their derivatives exhibit promising antimicrobial effects in vitro against G− and G+ bacteria that are resistant to antimicrobial drugs. The acid forms had stronger in vitro antimicrobial activities than ester forms, except that the medium chain fatty acid ester monolaurin exhibited strong inhibitory effects on G+ bacteria.

scholarly journals 169 Susceptibility of several species of gram-negative and gram-positive bacteria to organic acids and their derivatives

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 96-97
Author(s):  
Lauren Kovanda ◽  
Xunde Li ◽  
Wen Zhang ◽  
Xiaohong Wei ◽  
Jia Luo ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Organic Acids ◽  
Formic Acid ◽  
Butyric Acid ◽  
Sodium Formate ◽  
Valeric Acid ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative

Abstract The objective of this experiment was to determine the in vitro antimicrobial activity of several organic acids and their derivatives against gram-positive (G+) and gram-negative (G-) bacteria. Monopropionin, monovalerin, monolaurin, and sodium formate were tested at 10 to 12 concentrations from 0.001 to 2.50 mg/L; monobutyrin was tested at 16 concentrations from 0.001 to 5.00 mg/L; butyric acid and valeric acid were tested at 8 concentrations from 0.001 to 0.35 mg/L; while formic acid was tested at 8 concentrations from 0.001 to 1.00 mg/L. The tested bacteria included G- bacteria (Escherichia coli, Salmonella typhimurium, and Campylobacter jejuni) and G+ bacteria (Enterococcus faecalis, Clostridia perfringens, Streptococcus pneumonia, and Streptococcus suis). Antimicrobial activity of tested compounds was expressed as minimum inhibitory concentration (MIC) that prevented growth of tested bacteria in treated culture broth. Butyric acid, valeric acid, and formic acid at highest tested concentrations inhibited the growth of all bacterial strains. The MICs of the three acids varied among bacterial strains with lowest MIC of 0.05–0.10 mg/L on two strains of Campylobacter. Sodium formate at highest tested concentrations did not inhibit the growth of E. coli, S. typhimurium, and E. faecalis but the compound inhibited the growth of other tested bacteria with MIC values from 0.20 to 1.88 mg/L. The MIC values of monovalerin, monolaurin, and monobutyrin ranged between 0.001 and 1.50 mg/L on all bacterial strains with the exception that monobutyrin did not inhibit C. jejuni (ATCC 33560) growth at the highest concentration tested (5.0 mg/L). Monopropionin at tested concentrations did not inhibit the growth of all tested bacteria with the exception of 1.13 mg/L as MIC on C. perfringens (ATCC 12915). The MIC tests indicate that all organic acids and their derivatives, except monopropionin, tested in this experiment exhibited strong antimicrobial effects in vitro against tested bacterial strains.

scholarly journals Design, Synthesis, and Evaluation of Novel 3-, 4-substituted, and 3,4-di Substituted Quinazoline Derivatives as Antimicrobial Agents

2020 ◽  
Vol 71 (2) ◽  
pp. 422-435
Author(s):  
Farag A. El-Essawy ◽  
Abdulrahman I. Alharthi ◽  
Mshari A. Alotaibi ◽  
Nancy E. Wahba ◽  
Nader M. Boshta
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agents ◽  
Antimicrobial Activities ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Design Synthesis ◽  
Gram Negative ◽  
Spectroscopic Analyses ◽  
Quinazoline Derivatives

A novel series of 3-, 4-substituted, and 3,4-di substituted quinazoline derivatives were prepared via various cyclized regents and most of the newly prepared compounds evaluated for their antimicrobial activities in vitro against Gram-positive, Gram-negative bacterial strains and fungi strains. The structures of the quinazoline derivatives have been confirmed using spectroscopic analyses (IR, NMR, and EI-MS). Some of the synthesized derivatives displayed a moderate antimicrobial activity in comparison with reference drugs, for example compounds 13d, 15a, 17b, 18b, 18d, 25, and 29a-c. Among the synthesized compounds, the pyrimidoqunazoline derivative 6c elicited the highest activity.

Synthesis, Characterization and Antimicrobial Activity of Bis-Acetylferrocene Schiff Base

2011 ◽  
Vol 396-398 ◽  
pp. 1875-1878 ◽  
Author(s):  
Yu Ting Liu ◽  
Hai Long Guo ◽  
Da Wei Yin
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Salmonella Typhi ◽  
Bacterial Strains ◽  
Gram Negative ◽  
And Staphylococcus Aureus

Three bis-acetylferrocene schiff bases have been synthesized and characterized by IR, 1H NMR, and elemental analysis, the results conformed well with expected structures. The synthesized compounds were screened in vitro for their antimicrobial activity against three Gram-negative (Escherichia coli, Pseudomonas aeruginosa, and Salmonella typhi) and two Gram-positive (Bacillus subtilis and Staphylococcus aureus) bacterial strains. The results showed that these compounds are show excellent antimicrobia activities against Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi ,Bacillus subtilis,Staphylococcus aureus.

scholarly journals Antimicrobial Activity of Copper(II) and Cobalt(II) Complexes of Citral-Valine Derived Schiff Base

2019 ◽  
Vol 32 (1) ◽  
pp. 192-194
Author(s):  
S. Sudha Kumari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Schiff Base ◽  
Candida Albicans ◽  
Schiff Base Ligand ◽  
Antimicrobial Activities ◽  
Bacterial Strains ◽  
Gram Negative

In present work, the screening of antimicrobial activities of copper(II) and cobalt(II) complexes with Schiff base ligand derived from the condensation of citral with valine (amino acid) was carried out on agar plates are reported. The antibacterial activity of Schiff base and its copper(II) and cobalt(II) complexes were evaluated against two bacterial strains Staphylococcus aureus (Gram-positive), Escherichia coli (Gram-negative) and fungus Candida albicans. The results revealed that the Schiff base ligand exhibited the poor antimicrobial activity against Escherichia coli and Candida albicans except for Staphylococcus aureus. Generally, Gram-negative bacteria shows rigid outer membrane, well enough to defend against the drug but Schiff base (citral with valine derived) impregnated cobalt(II) complex seem to be more active against Escherichia coli organisms in comparison to copper(II) complex, which exhibits higher activity than uncomplexed ligand. The antimicrobial results revealed that cobalt(II) and copper(II) complexes have a considerable antibacterial activity than antifungal activity and suggest their potential application as antibacterial agents.

Synergistic Effects of Essential Oils of Ammoides verticillata and Satureja candidissima Against Many Pathogenic Microorganisms

2020 ◽  
Vol 18 (1) ◽  
pp. 72-78 ◽  
Author(s):  
Fatima Benyoucef ◽  
Mohammed El Amine Dib ◽  
Boufeldja Tabti ◽  
Arrar Zoheir ◽  
Jean Costa ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Synergistic Effects ◽  
Lactobacillus Rhamnosus ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Bacterial Strains ◽  
Gram Negative ◽  
Ammoides Verticillata

Background: Antibiotic resistance is today one of the most serious threats to global health, food security and development. Due to the growing number of infections, treatment becomes more difficult, if not impossible, because of the loss of antibiotic efficacy. Objective: In the present investigation, the chemical composition of essential oils of Ammoides verticillata and Satureja candidissima and their synergistic effects on antimicrobial activities were investigated. Methods: The chemical composition of the essential oil was analyzed by Gas Chromatography (GC) and Gas Chromatography-Mass Spectroscopy (GC/MS). The antimicrobial activity of the essential oils was investigated using dilution-agar method against nine bacterial strains three Gram-negative Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853) and Salmonella typhi (ATCC 14028), and six Gram-positive: Staphylococcus aureus (ATCC 43300,) Clostridium sporogenes (ATCC 19404), Bacillus subtilis (ATCC 6633), Enterococcus faecalis (ATCC 7314), Lactobacillus rhamnosus (ATCC 53103) and Bacillus cereus (ATCC 14579). Results: The essential oil of A. verticillata was characterized principally by carvacrol (44,3%), Limonene (19,3%) and p-cymene (19,2%). The constituents identified of S. candidissima essential oil were principally oxygenated monoterpenes represented by pulegone (70,4%). The essential oil of A. verticillata had a good antimicrobial activity against four bacterial strains (Escherichia coli, Salmonella typhi, Lactobacillus rhamnosus and Bacillus cereus) with MIC and MBC values between 0.2-0.4 µl/ml and 0.2-6.2 µl/ml, respectively. While, S. candidissima essential oil had moderate antimicrobial activities against all strains with MIC and MBC values between 1.5-6.2 µl/ml and 6.2-12.5 µl/ml, respectively. The results of antimicrobial activity of essential oils blend presented higher antimicrobial activity against all tested bacteria with MIC and MBC values between 0.3-1.5 µl/ml and 0.4-6.2 µl/ml, respectively. Conclusion: The essential oils blend presented high antimicrobial activity compared to virgin oils. This activity can be due to the association of active compounds such as carvacrol and pulegone. These findings provide a new source of drugs that may help in therapy to lead to the development of a new treatment based on a combination of these essential oils against gram-negative and gram-positive bacteria that continue to pose a threat to public health.

scholarly journals Antimicrobial Activity and DNA/BSA Binding Affinity of Polynuclear Silver(I) Complexes with 1,2-Bis(4-pyridyl)ethane/ethene as Bridging Ligands

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Sonja Ž. Đurić ◽  
Sandra Vojnovic ◽  
Tina P. Andrejević ◽  
Nevena Lj Stevanović ◽  
Nada D. Savić ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Therapeutic Potential ◽  
Fibroblast Cell ◽  
Bacterial Strains ◽  
Human Lung Fibroblast ◽  
Gram Negative ◽  
Minimal Inhibitory Concentrations ◽  
Lung Fibroblast Cell Line ◽  
Bacteria And Fungi

1,2-Bis(4-pyridyl)ethane (bpa) and 1,2-bis(4-pyridyl)ethene (bpe) were used for the synthesis of polynuclear silver(I) complexes, {[Ag(bpa)]NO3}n (1), {[Ag(bpa)2]CF3SO3.H2O}n (2) and {[Ag(bpe)]CF3SO3}n (3). In complexes 1–3, the corresponding nitrogen-containing heterocycle acts as a bridging ligand between two Ag(I) ions. In vitro antimicrobial activity of these complexes, along with the ligands used for their synthesis, was evaluated against the broad panel of Gram-positive and Gram-negative bacteria and fungi. The silver(I) complexes 1–3 showed selectivity towards Candida spp. and Gram-negative Escherichia coli in comparison to the other investigated bacterial strains, effectively inhibiting the growth of four different Candida species with minimal inhibitory concentrations (MICs) between 2.5 and 25 μg/mL and the growth of E. coli, with MIC value being 12.5 μg/mL. Importantly, complex 2 significantly reduced C. albicans filamentation, an essential process for its pathogenesis. Antiproliferative effect on the normal human lung fibroblast cell line MRC-5 was also evaluated with the aim of determining the therapeutic potential of the complexes 1–3. The interactions of these complexes with calf thymus DNA (ctDNA) and bovine serum albumin (BSA) were studied to evaluate their binding activities towards these biomolecules for possible insights on their mode of action.

scholarly journals Chemical Composition and Antimicrobial Activity of the Essential Oil from Chaerophyllum Aureum L. (Apiaceae)

2009 ◽  
Vol 4 (1) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Branislava Lakušić ◽  
Violeta Slavkovska ◽  
Milica Pavlović ◽  
Marina Milenković ◽  
Jelena Antić Stanković ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Micrococcus Luteus ◽  
Bacterial Strains ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Aerial Parts

The essential oils of the aerial parts and fruits of Chaerophyllum aureum L., collected from two mountains in Serbia, were analyzed by GC and GC/MS. Sabinene (18.5-31.6%), p-cymene (7.9-25.4%) and limonene (1.9-10.9%) were characterized as the main constituents. The oils were tested against six bacterial strains and one strain of yeast, Candida albicans. The highest antimicrobial activity was observed against the Gram-positive bacteria Staphylococcus aureus, S. epidermidis and Micrococcus luteus, while of the Gram-negative strains, Escherichia coli was the most sensitive.

scholarly journals In Vitro Bactericidal Activity of Human β-Defensin 3 against Multidrug-Resistant Nosocomial Strains

2006 ◽  
Vol 50 (2) ◽  
pp. 806-809 ◽  
Author(s):  
Giuseppantonio Maisetta ◽  
Giovanna Batoni ◽  
Semih Esin ◽  
Walter Florio ◽  
Daria Bottai ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bactericidal Activity ◽  
Stenotrophomonas Maltophilia ◽  
Bactericidal Effect ◽  
Multidrug Resistant ◽  
Bacterial Strains ◽  
Gram Negative

ABSTRACT The antimicrobial activity of human β-defensin 3 (hBD-3) against multidrug-resistant clinical isolates of Staphylococcus aureus, Enterococcus faecium, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Acinetobacter baumannii was evaluated. A fast bactericidal effect (within 20 min) against all bacterial strains tested was observed. The presence of 20% human serum abolished the bactericidal activity of hBD-3 against gram-negative strains and reduced the activity of the peptide against gram-positive strains.

scholarly journals Spatial Structure and Antimicrobial Activity of Cyclopropane Derivative of Limonene

2018 ◽  
Vol 13 (4) ◽  
pp. 1934578X1801300
Author(s):  
Daniyar Sadyrbekov ◽  
Timur Saliev ◽  
Yuri Gatilov ◽  
Ivan Kulakov ◽  
Roza Seidakhmetova ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Spatial Structure ◽  
Bacterial Strains ◽  
Gram Negative ◽  
X Ray ◽  
Cyclopropane Derivative

A cyclopropane derivative of limonene, (1 S, 4 S, 6 R)-7,7-dichloro-4-[(1 S)-2,2-dichloro-1-methylcyclopropyl]-1-methylbicyclo [4.1.0] heptane (compound 2), was synthesized and its structure was determined by NMR and X-ray crystallographic methods. In addition, an antimicrobial activity of the compound against Gram-positive ( Staphylococcus aureus, Bacillus subtilis) and Gram-negative ( Escherichia coli, Pseudomonas aeruginosa) bacterial strains was also scrutinized.

Activity of free fatty acids-rich non-polar fractions from fruits of Capsicum chinense var. Criolla and Jaguar

2019 ◽  
Vol 66 (3-4) ◽  
pp. 220-226
Author(s):  
J. Efrain Ramirez-Benitez ◽  
Ibis Vargas Paredes ◽  
Luis F. Cuevas Glory ◽  
Enrique Sauri Duch ◽  
Victor M. Moo Huchin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Staphylococcus Epidermidis ◽  
Bioactive Molecules ◽  
Biological Functions ◽  
Capsicum Chinense ◽  
Bacterial Strains ◽  
Gram Negative ◽  
Plant Essential Oils ◽  
Unripe Fruits

Plant-essential oils have been considered as an important source of bioactive molecules like antimicrobials, analgesics, anti-inflammatory and anti-carcinogen agents. Biological functions of plant extracts from the genus Capsicum are unknown. In the present work, non-polar fractions of ripe and unripe fruits of Capsicum chinense Jacq. Cultivar (cv.) Jaguar and Criollo were obtained by hexane-batch extraction and tested for antimicrobial activity against Gram-negative bacterial strain Escherichia coli (ATCC 25922), Gram-positive bacterial strains Enterococcus faecalis (ATCC 29212), Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 12228), and yeast Candida albicans (ATCC 90028). Non-polar fractions from ripe fruits for both cv. exhibited greater antimicrobial activity compared to unripe fruits. Implication of numbered FFA’s on observed antimicrobial activity are discussed.

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