scholarly journals Role and Function of MicroRNAs in Extracellular Vesicles in Cardiovascular Biology

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Philipp Pfeifer ◽  
Nikos Werner ◽  
Felix Jansen
Keyword(s):  
Extracellular Vesicles ◽  
The Body ◽  
Intercellular Signaling ◽  
Apoptotic Bodies ◽  
Vascular Integrity ◽  
Altered Protein ◽  
Fluid Compartment ◽  
Cardiovascular Biology ◽  
And Function ◽  
Role And Function

Intercellular communication mediated by extracellular vesicles is crucial for preserving vascular integrity and in the development of cardiovascular disease. Extracellular vesicles consist of apoptotic bodies, microvesicles, and exosomes that can be found in almost every fluid compartment of the body like blood, saliva, and urine. In the recent years, a lot of reports came up suggesting that major cardiovascular and metabolic pathologies like atherogenesis, heart failure, or diabetes are highly influenced by transfer of microRNAs via extracellular vesicles leading to altered protein expression and phenotypes of recipient cells. The following review will summarize the fast developing field of intercellular signaling in cardiovascular biology by microRNA-containing extracellular vesicles.

scholarly journals Extracellular Vesicles and Thrombosis: Update on the Clinical and Experimental Evidence

2021 ◽  
Vol 22 (17) ◽  
pp. 9317
Author(s):  
Konstantinos Zifkos ◽  
Christophe Dubois ◽  
Katrin Schäfer
Keyword(s):  
Experimental Evidence ◽  
Extracellular Vesicles ◽  
Thrombus Formation ◽  
Experimental Studies ◽  
Cell Types ◽  
Heterogenous Group ◽  
Clearance Mechanism ◽  
And Function ◽  
Role And Function

Extracellular vesicles (EVs) compose a heterogenous group of membrane-derived particles, including exosomes, microvesicles and apoptotic bodies, which are released into the extracellular environment in response to proinflammatory or proapoptotic stimuli. From earlier studies suggesting that EV shedding constitutes a cellular clearance mechanism, it has become evident that EV formation, secretion and uptake represent important mechanisms of intercellular communication and exchange of a wide variety of molecules, with relevance in both physiological and pathological situations. The putative role of EVs in hemostasis and thrombosis is supported by clinical and experimental studies unraveling how these cell-derived structures affect clot formation (and resolution). From those studies, it has become clear that the prothrombotic effects of EVs are not restricted to the exposure of tissue factor (TF) and phosphatidylserines (PS), but also involve multiplication of procoagulant surfaces, cross-linking of different cellular players at the site of injury and transfer of activation signals to other cell types. Here, we summarize the existing and novel clinical and experimental evidence on the role and function of EVs during arterial and venous thrombus formation and how they may be used as biomarkers as well as therapeutic vectors.

scholarly journals Cell-to-Cell Communication in Learning and Memory: From Neuro- and Glio-Transmission to Information Exchange Mediated by Extracellular Vesicles

2019 ◽  
Vol 21 (1) ◽  
pp. 266 ◽  
Author(s):  
Gabriella Schiera ◽  
Carlo Maria Di Liegro ◽  
Italia Di Liegro
Keyword(s):  
Learning And Memory ◽  
Extracellular Vesicles ◽  
Glial Cells ◽  
Information Exchange ◽  
System Development ◽  
Cell Communication ◽  
Nervous System Development ◽  
The Body ◽  
Pathological Conditions ◽  
And Function

Most aspects of nervous system development and function rely on the continuous crosstalk between neurons and the variegated universe of non-neuronal cells surrounding them. The most extraordinary property of this cellular community is its ability to undergo adaptive modifications in response to environmental cues originating from inside or outside the body. Such ability, known as neuronal plasticity, allows long-lasting modifications of the strength, composition and efficacy of the connections between neurons, which constitutes the biochemical base for learning and memory. Nerve cells communicate with each other through both wiring (synaptic) and volume transmission of signals. It is by now clear that glial cells, and in particular astrocytes, also play critical roles in both modes by releasing different kinds of molecules (e.g., D-serine secreted by astrocytes). On the other hand, neurons produce factors that can regulate the activity of glial cells, including their ability to release regulatory molecules. In the last fifteen years it has been demonstrated that both neurons and glial cells release extracellular vesicles (EVs) of different kinds, both in physiologic and pathological conditions. Here we discuss the possible involvement of EVs in the events underlying learning and memory, in both physiologic and pathological conditions.

scholarly journals Extracellular Vesicles from Caveolin-Enriched Microdomains Regulate Hyaluronan-Mediated Sustained Vascular Integrity

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Tamara Mirzapoiazova ◽  
Frances E. Lennon ◽  
Bolot Mambetsariev ◽  
Michael Allen ◽  
Jacob Riehm ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Extracellular Vesicles ◽  
Therapeutic Strategy ◽  
Extracellular Vesicle ◽  
The Body ◽  
Vascular Integrity ◽  
Barrier Disruption ◽  
Disease States ◽  
Barrier Regulation

Defects in vascular integrity are an initiating factor in several disease processes. We have previously reported that high molecular weight hyaluronan (HMW-HA), a major glycosaminoglycan in the body, promotes rapid signal transduction in human pulmonary microvascular endothelial cells (HPMVEC) leading to barrier enhancement. In contrast, low molecular weight hyaluronan (LMW-HA), produced in disease states by hyaluronidases and reactive oxygen species (ROS), induces HPMVEC barrier disruption. However, the mechanism(s) of sustained barrier regulation by HA are poorly defined. Our results indicate that long-term (6–24 hours) exposure of HMW-HA induced release of a novel type of extracellular vesicle from HLMVEC called enlargeosomes (characterized by AHNAK expression) while LMW-HA long-term exposure promoted release of exosomes (characterized by CD9, CD63, and CD81 expression). These effects were blocked by inhibiting caveolin-enriched microdomain (CEM) formation. Further, inhibiting enlargeosome release by annexin II siRNA attenuated the sustained barrier enhancing effects of HMW-HA. Finally, exposure of isolated enlargeosomes to HPMVEC monolayers generated barrier enhancement while exosomes led to barrier disruption. Taken together, these results suggest that differential release of extracellular vesicles from CEM modulate the sustained HPMVEC barrier regulation by HMW-HA and LMW-HA. HMW-HA-induced specialized enlargeosomes can be a potential therapeutic strategy for diseases involving impaired vascular integrity.

scholarly journals Skeletal muscle releases extracellular vesicles with distinct protein and miRNA signatures that accumulate and function within the muscle microenvironment

2021 ◽  
Author(s):  
Sho Watanabe ◽  
Yuri Sudo ◽  
Satoshi Kimura ◽  
Kenji Tomita ◽  
Makoto Noguchi ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Extracellular Vesicles ◽  
Cell Types ◽  
The Body ◽  
C2c12 Cells ◽  
Ips Cell ◽  
Potential Marker ◽  
And Function ◽  
Intercellular Communications

Extracellular vesicles (EVs) contain various regulatory molecules and mediate intercellular communications. Although EVs are secreted from various cell types, including skeletal muscle cells, and present in the blood, their identity is poorly characterized in vivo, limiting the identification of their origin in the blood. Since the skeletal muscle is the largest organ in the body, it could substantially contribute to circulating EVs as their source. However, due to the lack of defined markers that distinguish SkM-EVs from others, whether the skeletal muscle releases EVs in vivo and how much the skeletal muscle-derived EVs (SkM-EVs) account for plasma EVs remain poorly understood. In this work, we perform quantitative proteomic analyses on EVs released from C2C12 cells and human iPS cell-derived myocytes and identify potential marker proteins that mark SkM-EVs. These markers we identified apply to in vivo tracking of SkM-EVs. The results show that skeletal muscle makes only a subtle contribution to plasma EVs as their source in both control and exercise conditions in mice. On the other hand, we demonstrate that SkM-EVs are concentrated in the skeletal muscle interstitium. Furthermore, we show that interstitium EVs are highly enriched with the muscle-specific miRNAs and repress the expression of the paired box transcription factor Pax7, a master regulator for myogenesis. Taken together, our findings reveal that the skeletal muscle releases exosome-like small EVs with distinct protein and miRNA profiles in vivo and that SkM-EVs mainly play a role within the muscle microenvironment where they accumulate.

scholarly journals 332 Young Scholar Presentation: regulation of immune signaling by extracellular vesicles

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 132-133
Author(s):  
Caroline M Ylioja ◽  
Laman K Mamedova ◽  
Barry J Bradford
Keyword(s):  
Extracellular Vesicles ◽  
Immune Suppression ◽  
Immune Cells ◽  
The Body ◽  
Metabolic Dysfunction ◽  
Mediated Communication ◽  
Transition Cow ◽  
Cow Health ◽  
And Function ◽  
High Degree

Abstract Exosomes and other extracellular vesicles traffic a variety of protein and nucleic acid cargo throughout the body. Immune cells especially utilize these vesicles for communication, and they are being studied in clinical settings for their potential to act as disease markers, but also to serve as drug delivery systems. In animal science, a large part of the research around exosomes has centered around their presence in milk, as well as their bioavailability and potential impact for the neonate and for humans; milk-derived exosomes are enriched with immune-related proteins and microRNA that may relate to maternal health, mammary function, or neonate development. We sequenced miRNA isolated from colostrum of dairy cows predicted to feature either moderate or high degree of immune suppression, but found minimal differences (2 differentially expressed miRNA of 343 miRNA analyzed) between the two groups. Immune suppression exhibited during early lactation may also be related to altered exosome-mediated communication between immune cells. We studied the ability of bovine exosomes to alter immune responses of primary bovine monocyte-derived macrophages and found that exosomes alone or in combination with LPS were able to stimulate cytokine production to a greater extent than LPS alone. The ability of exosomes to transport cytokine, bioactive lipid, and regulatory RNA cargo suggests potential involvement in transition cow health. Altered exosome content and function have been associated with uterine infection, mastitis, and metabolic dysfunction; future studies with exosome signaling may help clarify the complexities of transition cow immune function and point to strategies to support immunity.

scholarly journals Tinjauan Realitas Terhadap Status, Peran Dan Fungsi Manusia

CICES ◽  
2015 ◽  
Vol 1 (1) ◽  
pp. 50-60
Author(s):  
Mohammad Urfan ◽  
Desy Apriani ◽  
Dedeh Supriyanti
Keyword(s):  
The Body ◽  
Human Beings ◽  
The World ◽  
And Function ◽  
Role And Function

In the letter 12-14 Verse 23 of Qoran, it describes the process of creation of humanfrom nuthfah, turned into alaqah, mudhghah, Izham and khalqan akhar (other beings).Further, It has described in other verses like (QS:25:54), (QS:86:6-7), (QS:18:37),(QS:32:7), (QS: 37:11), (QS:15:26). After learning carefully the various verses above, wecan conclude that the body / physical is one of element that exists within the human.However, these elements have many weaknesses. The purpose of this study was toexamine the three elements that exist in human beings, then we would like to observecarefully about status, role and function of one of the most important elements in humman,which causes humans can be regarded as a perfect creature. The methods of thisobservation is examines various verses in the Qoran related to human, then we would liketo force the reader to trust and believe in the truth. Results of this study prove that humanis composed of three elements, namely the bodies (physical materiality), spirit and soul.The study concluded that soul that distinguishes human from other creatures. The soul thatcan make a human become perfect beings. Soul has been main actors and controllers all ofhuman life in the world.

Masyarakat Mantap

2020 ◽  
Vol 2 (2) ◽  
pp. 45-50
Author(s):  
Siti Maryam Qurotul Aini ◽  
Sukandar Sukandar ◽  
Hafidul Umami
Keyword(s):  
Community Development ◽  
The Body ◽  
Community Leaders ◽  
Community Based ◽  
Community Based Education ◽  
Asset Based Community Development ◽  
Development Approach ◽  
And Function ◽  
Role And Function

The role and function of the mosque in rural Takmir often still dwell on the management and service, has not touched on the empowerment efforts of pilgrims. Takmir Mosque has the opportunity to develop community based education. In this case the role of the college in the mentoring of the mosque Takmir indispensable. This mentoring uses an ABCD (asset based community Development) approach conducted in Jatipunggur Lengkong Nganjuk village. The result of mentoring managed to realize community based education in the form of empowering the mosque Takmir to build worshippers in terms of the care of the body and to overcome femininity problems. This achievement can be achieved because the process of mentoring from the stage of social analysis, planning, implementation and evaluation is done with all the mosque stakeholders. Takmir Mosque managed to get mentoring to organize community based education by involving administrators, village apparatus and religious and community leaders.

scholarly journals Extracellular Vesicles in CNS Developmental Disorders

2020 ◽  
Vol 21 (24) ◽  
pp. 9428
Author(s):  
Ana Rita Gomes ◽  
Nasim Bahram Sangani ◽  
Tiago G. Fernandes ◽  
M. Margarida Diogo ◽  
Leopold M. G. Curfs ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Developmental Disorders ◽  
Complex Structure ◽  
Cell Types ◽  
The Body ◽  
Neural Cells ◽  
Cytoplasmic Proteins ◽  
Gene And Protein Expression ◽  
Multiple Cell ◽  
And Function

The central nervous system (CNS) is the most complex structure in the body, consisting of multiple cell types with distinct morphology and function. Development of the neuronal circuit and its function rely on a continuous crosstalk between neurons and non-neural cells. It has been widely accepted that extracellular vesicles (EVs), mainly exosomes, are effective entities responsible for intercellular CNS communication. They contain membrane and cytoplasmic proteins, lipids, non-coding RNAs, microRNAs and mRNAs. Their cargo modulates gene and protein expression in recipient cells. Several lines of evidence indicate that EVs play a role in modifying signal transduction with subsequent physiological changes in neurogenesis, gliogenesis, synaptogenesis and network circuit formation and activity, as well as synaptic pruning and myelination. Several studies demonstrate that neural and non-neural EVs play an important role in physiological and pathological neurodevelopment. The present review discusses the role of EVs in various neurodevelopmental disorders and the prospects of using EVs as disease biomarkers and therapeutics.

scholarly journals Nano-Vesicle (Mis)Communication in Senescence-Related Pathologies

Cells ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1974
Author(s):  
Sherin Saheera ◽  
Ajay Godwin Potnuri ◽  
Prasanna Krishnamurthy
Keyword(s):  
Extracellular Vesicles ◽  
Biological Fluids ◽  
Critical Role ◽  
Biological Information ◽  
Multivesicular Bodies ◽  
Apoptotic Bodies ◽  
Signaling Crosstalk ◽  
Progressive Decline ◽  
Exosome Biogenesis ◽  
And Function

Extracellular vesicles are a heterogeneous group of cell-derived membranous structures comprising of exosomes, apoptotic bodies, and microvesicles. Of the extracellular vesicles, exosomes are the most widely sorted and extensively explored for their contents and function. The size of the nanovesicular structures (exosomes) range from 30 to 140 nm and are present in various biological fluids such as saliva, plasma, urine etc. These cargo-laden extracellular vesicles arise from endosome-derived multivesicular bodies and are known to carry proteins and nucleic acids. Exosomes are involved in multiple physiological and pathological processes, including cellular senescence. Exosomes mediate signaling crosstalk and play a critical role in cell–cell communications. Exosomes have evolved as potential biomarkers for aging-related diseases. Aging, a physiological process, involves a progressive decline of function of organs with a loss of homeostasis and increasing probability of illness and death. The review focuses on the classic view of exosome biogenesis, biology, and age-associated changes. Owing to their ability to transport biological information among cells, the review also discusses the interplay of senescent cell-derived exosomes with the aging process, including the susceptibility of the aging population to COVID-19 infections.

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