scholarly journals Role of Green Tea in Reducing Epidermal Thickness upon Ultraviolet Light-B Injury in BALB/c Mice

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Snur M. A. Hassan ◽  
Adel J. Hussein ◽  
Azad K. Saeed
Keyword(s):  
Green Tea ◽  
Uv Radiation ◽  
Antioxidant Properties ◽  
Control Group ◽  
Skin Damage ◽  
Epidermal Thickness ◽  
Solar Ultraviolet Radiation ◽  
Uvb Irradiation ◽  
Uvb Exposure ◽  
Ultraviolet Light B

The main environmental source for skin damage is ultraviolet (UV) radiation. Many adverse effects have been recognized as the result of prolonged cutaneous exposure to solar ultraviolet radiation, such as erythema, edema, apoptosis, hyperplastic responses, photo-aging, and skin cancer development. Green tea provides photo-protection against UV radiation through many mechanisms including anti-inflammatory, immunomodulatory, and antioxidant properties. The aim of this study was to evaluate the effect of green tea in reducing epidermal thickness on mouse’s skin exposed to UVB irradiation. Thirty mice (Mus musculus species, BALB/c strain) underwent this study and were divided into 3 groups: control group (n=10 mice), without UVB exposure and green tea administration; exposure group (n=10 mice), which were exposed to UVB light only; and treatment group (n=10 mice), which were exposed to UVB light and treated with 1 mL of green tea through oral gavage. Mice from both groups (exposure and treatment) were subjected to UVB irradiation 4 days/week (20 minutes/day, 4 weeks). It concluded that oral administration of green tea was provided photo-protection against UVB induced hyperplasia; therefore, it can be regarded as a natural alternative for photo-protection.

scholarly journals Laminarin Attenuates Ultraviolet-Induced Skin Damage by Reducing Superoxide Anion Levels and Increasing Endogenous Antioxidants in the Dorsal Skin of Mice

Marine Drugs ◽  
2020 ◽  
Vol 18 (7) ◽  
pp. 345 ◽  
Author(s):  
Ji Hyeon Ahn ◽  
Dae Won Kim ◽  
Cheol Woo Park ◽  
Bora Kim ◽  
Hyejin Sim ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Anion ◽  
Collagen Fibers ◽  
Ultraviolet B ◽  
Control Group ◽  
Dorsal Skin ◽  
Skin Damage ◽  
Superoxide Anion Production ◽  
Uvb Exposure ◽  
Immunohistochemical Studies

A number of studies have demonstrated that marine carbohydrates display anti-oxidant, anti-melanogenic, and anti-aging activities in the skin. Laminarin (LA), a low-molecular-weight polysaccharide, is found in brown algae. The benefits of LA in ultraviolet B (UVB) induced photodamage of the skin have not been reported. The aim of this study was to investigate the effects of pre-treated LA on histopathological changes and oxidative damage in mouse dorsal skin on day 5, following repeated UVB exposure. Histopathology, Western blot analysis and immunohistochemical studies showed that epidermal thickness in the UVB group was significantly increased; however, the thickness in the UVB group treated with LA (LA/UVB group) was less compared with that of the UVB group. Collagen fibers in the dermis of the UVB group were significantly decreased and destroyed, whereas, in the LA/UVB group, the density of collagen fibers was significantly increased compared with that of the UVB group. Oxidative stress due to superoxide anion production measured via dihydroethidium fluorescence staining was dramatically increased in the UVB group, whereas in the LA/UVB group, the oxidative stress was significantly decreased. Expressions of SOD1, glutathione peroxidase and catalase were markedly reduced in the UVB group, whereas in the LA/UVB group, they were significantly higher along with SOD2 than in the control group. Taken together, our results indicate that LA pretreatment prevents or attenuates skin damage, by decreasing oxidative stress and increasing antioxidant enzymes in mouse dorsal skin.

scholarly journals The Difference between the Effect of Green Tea Cream and Tocopherol on Decreasing Level of Tyrosinase Enzyme and Amount of Melanin in Rattus norvegicus Exposed to UVB Rays

Sains Medika ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 20
Author(s):  
Anwar Riyanto ◽  
Taufiqurrachman Nasihun ◽  
Titiek Sumarawati
Keyword(s):  
Green Tea ◽  
Wistar Rats ◽  
Control Group ◽  
Topical Cream ◽  
Male Wistar Rats ◽  
The Difference ◽  
Tyrosinase Enzyme ◽  
Uvb Exposure ◽  
Post Hoc ◽  
Better Than

Introduction: Green tea and tocopherol are potent antioxidants used to treat melasma. However, whether green tea or tocopherol is superior remains unclear. Objective: To compare the effectiveness between green tea and tocopherol in decreasing the amount of melanin and tyrosinase level in wistar rats exposed to UVB rays.Methods: The experimental study took 30 male Wistar rats randomly and divided them into 3 groups. The control group (C-G) was given basic topical cream, GT-G was given green tea topical cream and TC-G was given tocopherol topical cream. All rats were exposed to UVB every Monday, Wednesday and Friday for 4 weeks, whereas topical creams were smeared every day. Topical creams smearing on the same day with UVB exposure was performed 20 minutes before exposure and 4 hours after UVB exposure. The doses of UVB were 50 mJ/cm2 in first week, 70mJ/cm2 in the second week and 80mJ/cm2 in the third and fourth weeks. The amount of melanin was measured using pixel method and the tyrosinase level was measured using ELISA.Results: Anova analysis indicates that the amount of melanin and tyrosinase level are significantly different between groups, p<0.05. Post Hoc LSD analysis indicates that the amount of melanin in GT-G and TC-G are significantly lower than that of C-G, p<0.05. The amount of melanin in GT-G is lower than that of TC-G, p<0.05. The tyrosinase level in GT-G is significantly lower than that of C-G and TC-G, p<0.05. Meanwhile, the tyrosinase level in TC-G is lower than that of C-G but insignificantly, p>0.05.� Conclusion: Green tea topical treatment is significantly capable of decreasing the amount of melanin and tyrosinase level better than tocopherol.

scholarly journals Mirabilis Jalapa L. Protein as Inductor of Apoptosis on UVB-induced Skin Damage in Mice

2017 ◽  
Vol 16 (3) ◽  
pp. 423-427
Author(s):  
Atina Hussaana ◽  
Sismindari ◽  
Sitarina Widyarini ◽  
Sudjadi ◽  
Zullies Ikawati
Keyword(s):  
Protective Effect ◽  
Dna Fragmentation ◽  
Caspase 3 ◽  
Control Group ◽  
Peak Time ◽  
Uvb Radiation ◽  
Skin Damage ◽  
Uvb Irradiation ◽  
L Protein ◽  
Mirabilis Jalapa

Background: Mirabilis jalapa L. protein (MJ-Protein) has been shown to have antioxidant and anti-inflammatory effects in vitro. Thus, it has a potential protective effect against ultraviolet B (UVB)-induced skin damage.Objective: To determine the protective effect and mechanism of MJ protein in UVB-radiation exposed mouse skin.Methods: In this experimentalstudy, 30 female BALB/c mice aged 6 weeks were exposed to a single dose of UVB irradiation with 3 minimal erythema doses (MEDs) and continued with the treatment of 0.6 mg MJ-Protein topically. The number of apoptotic body (sunburn cells) formed in epidermal layers of mouse dorsal skin was assessed at 1, 24, 48, 72, 96 and 120h after UVB irradiation was compared to that of the control group. The difference in the sunburn cells number between two groups were analyzed using independent T-test with the level of significance of 0.05. The apoptosis mechanism was confirmed qualitatively by caspase-3 and DNA fragmentation analysis in vitro.Results:At 24 h after the UVB exposure (peak time for sunburn cells formation), there was a significant increase in the sunburn cells number in the group treated with topical application of MJ-Protein. There was increased caspase-3 expression and DNA fragmentation in HeLa cells treated with MJ-Protein.Conclusions: MJ-Protein protects againts UVB-induced skin damage in mice trough apoptosis induction.Bangladesh Journal of Medical Science Vol.16(3) 2017 p.423-427

scholarly journals Echinacoside Alleviates UVB Irradiation-Mediated Skin Damage via Inhibition of Oxidative Stress, DNA Damage, and Apoptosis

2017 ◽  
Vol 2017 ◽  
pp. 1-15 ◽  
Author(s):  
Di Zhang ◽  
Chengtao Lu ◽  
Zhe Yu ◽  
Xiayin Wang ◽  
Li Yan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Histopathological Examination ◽  
Ultraviolet B ◽  
Ros Generation ◽  
Antioxidant Enzyme Activities ◽  
Skin Damage ◽  
Uvb Irradiation ◽  
Uvb Exposure

Ultraviolet B (UVB) irradiation has been known to cause skin damage, which is associated with oxidative stress, DNA damage, and apoptosis. Echinacoside is a phenylethanoid glycoside isolated from Herba Cistanches, which exhibits strong antioxidant activity. In this study, we evaluate the photoprotective effect of echinacoside on UVB-induced skin damage and explore the potential molecular mechanism. BALB/c mice and HaCaT cells were treated with echinacoside before UVB exposure. Histopathological examination was used to evaluate the skin damage. Cell viability, lactate dehydrogenase (LDH) levels, antioxidant enzyme activities, reactive oxygen species (ROS) generation, DNA damage, and apoptosis were measured as well. Western blot was used to measure the expression of related proteins. The results revealed that pretreatment of echinacoside ameliorated the skin injury; attenuated oxidative stress, DNA damage, and apoptosis caused by UVB exposure; and normalized the protein levels of ATR, p53, PIAS3, hnRNP K, PARP, and XPA. To summarize, echinacoside is beneficial in the prevention of UVB-induced DNA damage and apoptosis of the skin in vivo and in vitro.

scholarly journals Evaluation of the Antioxidant Properties of Vitellaria paradoxa Seed Extract and Its Effect of Ultra Violet Radiation Induced Skin Damage

2020 ◽  
pp. 1-7
Author(s):  
Favour Moses Idih ◽  
Uyo Ruth Jeremiah ◽  
Hafsat Enesi ◽  
Oluseyi Valerie Alagbe
Keyword(s):  
Antioxidant Properties ◽  
Ultra Violet ◽  
Negative Control ◽  
Seed Extract ◽  
Control Group ◽  
Skin Damage ◽  
Vitellaria Paradoxa ◽  
Ultra Violet Radiation ◽  
Group 2 ◽  
Violet Radiation

Ultra violet radiation (UVR) is relevant in nature, but despite its importance it has been labeled as a causative agent in skin damage. This study was done to evaluate the antioxidant properties of shea butter and its role in the prevention of skin damage. Six groups of albino rats with three rats per group were used, group 2-5 were exposed to UVR at the dose of 180 mJ/cm2 with group 1 serving as the normal control, group 2 (negative control), group 2-5 (treated groups) and group 6 (normal + treated). The treatment was done by topical application of Vitellaria parodoxa seed extract 5 minutes after exposure to UVR. The in-vitro antioxidant properties determined using DPPH radical scavenging activity revealed that the aqueous extract of Vitellaria paradoxa possesses high antioxidant activity. There was an increase in the concentration of GSH of the treated animals when compared to the negative control (p<0.05). The histopathology result shows the extract had a protective effect on the skin tissue of the experimental animals. This study suggests the extract has the potential to serve as a chemopreventive agent against UV-induced skin damage by neutralizing the effects of free radicals generated by ultraviolet radiation and as such can be recommended for use as UVR screen which could in turn reduce the risk of UVR skin damage.

scholarly journals Protective effect of total flavonoids from boxthorn leaf against UVB irradiation-induced skin injury

2021 ◽  
Vol 18 (9) ◽  
pp. 1943-1947
Author(s):  
Qing Yu ◽  
Ying Shen ◽  
Yadan Gan ◽  
Liang Zheng
Keyword(s):  
Protective Effect ◽  
Negative Control Group ◽  
Negative Control ◽  
Ultraviolet B ◽  
Control Group ◽  
Total Flavonoids ◽  
Skin Damage ◽  
Skin Injury ◽  
Uvb Irradiation ◽  
Base Group

Purpose: To investigate the protective effect of total flavonoids from boxthorn leaf against skin injury induced by UVB irradiation, and to elucidate the underlying mechanism of action. Method: Healthy female mice (n = 100) were randomly divided into four groups: normal control group, UV negative control group, cream base group, and boxthorn leaf total flavonoid (BLTF) group, with 25 mice in each group. The mice in each group were irradiated with ultraviolet B (UVB) irradiation instrument for 1.5 h daily for 3 weeks. Mice in the cream base group were smeared with cream base on their backs, while mice in BLTF group were smeared with 15 mg/g boxthorn BLTF cream. The control and negative control group mice were not treated. Changes in superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) levels were determined using standard methods. Results: Compared to the negative control group, the levels of SOD and GSH-Px in the control group and BLTF were significantly elevated, while MDA levels declined significantly (p < 0.05). Although higher GSH-Px and SOD levels, and lower MDA were seen in the cream base group than in negative control group, these indices were comparable for the two groups (p > 0.05). Conclusion: The total flavonoids of boxthorn leaves improve resistance to UVB-induced skin damage by regulating SOD, MDA and GSH-Px levels in the skin of mice. Thus, they exert protective effects on the skin.

Effects of UV Rays and Thymol/Thymus vulgaris L. Extract in an ex vivo Human Skin Model: Morphological and Genotoxicological Assessment

2016 ◽  
Vol 201 (3) ◽  
pp. 180-192 ◽  
Author(s):  
Laura Cornaghi ◽  
Francesca Arnaboldi ◽  
Rossella Calò ◽  
Federica Landoni ◽  
William Franz Baruffaldi Preis ◽  
...  
Keyword(s):  
Human Skin ◽  
Uv Radiation ◽  
Ex Vivo ◽  
Protective Action ◽  
Thymus Vulgaris ◽  
Skin Damage ◽  
Normal Human Skin ◽  
Transmission Electron Microscopy Analysis ◽  
Lactate Dehydrogenase Activity ◽  
Uvb Exposure

Ultraviolet (UV) radiation is the major environmental factor affecting functions of the skin. Compounds rich in polyphenols, such as Thymus vulgaris leaf extract and thymol, have been proposed for the prevention of UV-induced skin damage. We compared the acute effects induced by UVA and UVB rays on epidermal morphology and proliferation, cytotoxicity, and genotoxicity. Normal human skin explants were obtained from young healthy women (n = 7) after informed consent and cultured at the air-liquid interface overnight. After 24 h, the samples were divided in 2 groups: the former exposed to UVA (16 or 24 J/cm2) and the latter irradiated with UVB (0.24 or 0.72 J/cm2). One hour after the end of irradiation, supernatants were collected for evaluation of the lactate dehydrogenase activity. Twenty-four hours after UVB exposure, biopsies were processed for light and transmission electron microscopy analysis, proliferation, cytotoxicity, and genotoxicity. UVB and UVA rays induced early inhibition of cell proliferation and DNA damage compared to controls. In particular, UVB rays were always more cytotoxic and genotoxic than UVA ones. For this reason, we evaluated the effect of either T. vulgaris L. extract (1.82 µg/ml) or thymol (1 µg/ml) on all samples treated for 1 h before UVB irradiation. While Thymus had a protective action for all of the endpoints evaluated, the action of the extract was less pronounced on epidermal proliferation and morphological features. The results presented in this study could be the basis for investigating the mechanism of thymol and T. vulgaris L. extract against the damage induced by UV radiation.

scholarly journals Potential Use of Amla (Phyllanthus emblica L.) Fruit Extract to Protect Skin Keratinocytes from Inflammation and Apoptosis after UVB Irradiation

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 703
Author(s):  
Khwandow Kunchana ◽  
Wattanased Jarisarapurin ◽  
Linda Chularojmontri ◽  
Suvara K. Wattanapitayakul
Keyword(s):  
Antioxidant Properties ◽  
Ultraviolet B ◽  
H2o2 Production ◽  
Phyllanthus Emblica ◽  
Cytochrome C Release ◽  
Fruit Extract ◽  
Antioxidant Power ◽  
Uvb Irradiation ◽  
Uvb Exposure ◽  
Potential Use

Ultraviolet B (UVB) exposure is the primary risk factor for the deadliest type of skin cancer—melanoma. Incorporating natural antioxidants in skin protection products is currently a favored research theme. For this study, we selected Phyllanthus emblica L. fruit extract (PE) to assess its potential use in dermal protection against UVB-induced keratinocyte inflammation and apoptosis. High-performance liquid chromatography (HPLC) was used to investigate PE’s phytochemical constituents (ascorbic acid, ellagic acid, gallic acid, chlorogenic acid, and quercetin), while ferric reducing antioxidant power (FRAP), oxygen radical absorbance capacity (ORAC), total ROS, OH•, O2•−, and H2O2-scavenging activities were used to determine the antioxidant properties. PE significantly increased the cell viability (MTT assay) and reduced apoptosis (Hoechst staining) in HaCaT cells exposed to UVB (40 mJ/cm2). PE abolished oxidative stress by reducing the production of intracellular ROS, O2•− and H2O2 production. Catalase activity (but not superoxide dismutase or glutathione peroxidase activity) was enhanced in keratinocytes incubated with PE prior to UVB exposure. Western blot analysis suggested that PE inhibited cytochrome c release and inhibited the dysregulation of PI3K/Akt without any impact on p38 activation. PE attenuated the inflammatory response to UVB irradiation by inhibiting AP-1, NF-κB, and the mediator PGE2. Thus, PE is a candidate with great potential for use as an active ingredient in skin care products.

scholarly journals Protective effects of Oxya chinensis sinuosa Mishchenko against ultraviolet B-induced photodamage in hairless mice

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
A-Rang Im ◽  
InWha Park ◽  
Kon-Young Ji ◽  
Joo Young Lee ◽  
Ki Mo Kim ◽  
...  
Keyword(s):  
Inflammatory Cytokine ◽  
Vehicle Control ◽  
Cytokine Expression ◽  
Ultraviolet B ◽  
Skin Hydration ◽  
Control Group ◽  
Protective Effects ◽  
Epidermal Thickness ◽  
Vehicle Control Group ◽  
Uvb Irradiation

Abstract Background Edible insects, including Oxya chinensis sinuosa Mishchenko (Oc), which is consumed as food in Asia, are considered as a human food shortage alternative, and also as a preventive measure against environmental destruction. Ultraviolet B (UVB) irradiation, which causes skin photodamage, is considered as an extrinsic skin aging factor. It reduces skin hydration, and increases wrinkle formation and reactive oxygen species (ROS) and inflammatory cytokine expression. Thus, the objective of this study was to investigate the anti-aging effects of an ethanol extract of Oc (Oc.Ex). Methods A UVB-irradiated hairless mouse model was used to examine relevant changes in skin hydration, wrinkle formation, and skin epidermal thickness. Also, antioxidant markers such as superoxide dismutase (SOD) and catalase (CAT) were analyzed, and Oc. Ex skin protective effects against UVB irradiation-induced photoaging were examined by determining the levels of skin hydration factors. Results Oc.Ex improved epidermal barrier dysfunctions such as increased transepidermal water loss (TEWL) and capacitance reduction in UVB-irradiated mice. It upregulated skin hydration-related markers, including hyaluronic acid (HA), transforming growth factor (TGF)-β, and pro-collagen, in UVB-irradiated mice, compared with the vehicle control group. It also reduced UVB-induced wrinkle formation, collagen degradation, and epidermal thickness. Additionally, it remarkably suppressed the increased expression of matrix metalloproteinases (MMPs), and restored the activity of SOD and CAT in UVB-irradiated mice, compared with the vehicle control group. Furthermore, Oc. Ex treatment downregulated the production of inflammatory cytokines and phosphorylation of the mitogen-activated protein kinases (MAPKs) signaling pathway activated by UVB irradiation. Conclusion This study revealed that Oc. Ex reduced skin thickness and the degradation of collagen fibers by increasing hydration markers and collagen-regulating factors in the skin of UVB-irradiated mice. It also inhibited UVB-induced antioxidant enzyme activity and inflammatory cytokine expression via MAPK signaling downregulation, suggesting that it prevents UVB-induced skin damage and photoaging, and has potential for clinical development in skin disease treatment.

scholarly journals The antioxidant role of Sideritis caesarea infusion against TCA toxicity in rats

2010 ◽  
Vol 105 (5) ◽  
pp. 663-668 ◽  
Author(s):  
Ismail Celik ◽  
Mehmet Salih Kaya
Keyword(s):  
Superoxide Dismutase ◽  
Green Tea ◽  
Antioxidant Properties ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Aerial Parts ◽  
Mda Content ◽  
Group B ◽  
The Brain

Sideritis caesarea (SC) Duman, Aytac&Baser is a member of the Lamiaceae family. The present study was designed to investigate the antioxidant properties of the aerial parts of island green tea SC against TCA effects in rats. Biomarkers selected for monitoring antioxidant capacity were the activities of glutathione reductase (GR), superoxide dismutase (SOD), glutathione-S-transferase, catalase (CAT), GSH level and malondialdehyde (MDA) content in various organs of rats. Three experimental groups, A (untreated = control), B (only TCA-treated) and C (TCA+SC-treated), were studied. At the end of the 50 d experiment, the MDA content in tissues increased significantly in group B, whereas no significant changes were observed in group C as compared with that of the control group. Antioxidant enzyme activities such as SOD and CAT increased significantly in the brain, liver and kidneys of group B but decreased significantly in group C as compared with group B. The GSH level and GR activity increased significantly in the brain and liver of group C as compared with the control and TCA-exposed rats. Hence, the study reveals that the constituents present in SC impart protection against chemical-induced oxidative injury that may result in the development of cancer.

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