scholarly journals Demucilaging Freshly Stored Seeds of Cocoa (Theobroma cacao L.) Improves Seedling Emergence and Growth

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Michael O. Adu ◽  
Thomas Cobbinah ◽  
Paul A. Asare ◽  
David O. Yawson ◽  
Kingsley J. Taah

Fresh seeds of cocoa (Theobroma cacao L.) are covered by a large amount of mucilage, which plays important ecological roles. However, for stored seeds, mucilage can adversely affect germination. This study investigated the effect of mucilage removal (demucilaging) and seed storage of freshly extracted cocoa seeds on seedling emergence and growth. The study showed that demucilaging cocoa seeds confers several advantages over mucilage intact seeds provided the seeds have been stored for at least three days before sowing. Demucilaging increased germination rate (T50) and percentage germination and presence of mucilage increased days to seedling emergence and cessation of germination when seeds were stored for over two days. For up to 21 days of seed storage, there was generally no difference in many traits for demucilaged seeds. Demucilaged seeds had 80–100% germination after 21 days of storage, and rate of germination increased with increasing duration of seed storage. Intact mucilage seeds maintained 80–100% germination for only 2 days after seed extraction and rate of germination decreased with increasing seed storage duration. We conclude that (i) if seeds are sown on the day of extraction demucilaging before sowing would be superfluous and (ii) demucilaging and storage can provide advantages to smallholder farmers when environmental or socioeconomic conditions preclude immediate sowing.

2018 ◽  
Vol 3 (2) ◽  
pp. 89
Author(s):  
Yulistiati Nengsih

Cocoa seeds are included in recalcitrant seeds that have the characteristic of aging and regrowth of seeds during storage, viability of seeds decreases when their water content is decreased (12-31%) and can not stand to be stored at low temperature and humidity. For the provision of quality seeds need to be mastered technology seed storage properly. The aim of this research is to get the proper packing technique in maintaining viability of cocoa seed (Theobroma cacao L) during storage. The research will be conducted from Pebruari to Juni 2018, at Batanghari University Basic Laboratory. The design used is the Randomized Complete environment with the treatment of cardboard packaging with several ventilation holes: P0 = cardboard without ventilation, P1 = 2% ventilation box, P2 = 4% ventilation box, P3 = 6% ventilation box and P4 = cardboard box 8 %. The observed variables were: percentage of moisture content, percentage of seeds germinated in storage, percentage of sprout gain after storage, germination rate after storage, percentage of moldy seeds in storage and identification mushrooms. To see the effect, F test was done at 5% level, then continued with Duncan test at ? 5%. The result of the research showed that the treatment of the packing ventilation had an effect on the percentage parameter of moldy and germinated seed in storage and seed viability and vigor. In the treatment between ventilation, did not show any difference, but economically recommended the use of ventilation by 8%. Until storage on day 12 of the treatment of packaging vents can withstand the fungus attack 17.19% compared to control. The amount of ventilation does not show ability to withstand viability and vigor after storage. The fungus identified in seed storage is Fusarium spp. and Aspergillus sppKeywords: Recalcitrant, viability, moisture content of seedsAbstrakBenih kakao termasuk benih rekalsitran yang mempunyai sifat mengalami penuaan dan kemunduran benih selama penyimpanan, viabilitas benih menurun apabila diturunkan kadar airnya (12-31%) dan tidak tahan disimpan pada suhu dan kelembaban rendah. Untuk pengadaan benih berkualitas perlu dikuasai teknologi penyimpanan benih secara tepat. Penelitian bertujuan untuk mendapatkan teknik pengemasan yang tepat dalam mempertahankan viabilitas benih kakao (Theobroma cacao. L) selama penyimpanan. Penelitian dilaksanakan pada bulan Pebruari sampai Juni 2018, di Laboratorium Dasar Universitas Batanghari. Rancangan yang digunakan adalah rancangan lingkungan Acak Lengkap dengan perlakuan kemasan kardus dengan beberapa lubang ventilasi yaitu : P0= kardus tanpa ventilasi, P1= kardus ventilasi 2%, P2= kardus ventilasi 4%, P3= kardus ventilasi 6 % dan P4= kardus ventilasi 8%. Peubah yang diamati adalah: Kadar air benih, persentase benih yang berkecambah dalam penyimpanan, persentase daya kecambah setelah penyimpanan, kecepatan berkecambah setelah penyimpanan, persentase benih berjamur dalam penyimpanan dan identifikasi jamur. Untuk melihat pengaruh perlakuan, dilakukan uji F pada taraf 5%, kemudian dilanjutkan dengan uji Duncan pada taraf ? 5%. Hasil penelitian menunjukkan bahwa perlakuan ventilasi pengemasan berpengaruh terhadap peubah persentase benih berjamur dan berkecambah dalam penyimpanan serta viabilitas dan vigor benih. Pada perlakuan antar ventilasi, tidak menunjukkan adanya perbedaan, akan tetapi secara ekonomis dianjurkan penggunaan ventilasi sebesar 8%. Sampai penyimpanan pada hari ke-12 perlakuan ventilasi kemasan dapat menahan serangan jamur 17,19% dibandingkan kontrol. Jumlah ventilasi tidak menunjukkan mampu menahan viabilitas dan vigor setelah penyimpanan. Jamur yang teridentifikasi dalam penyimpanan benih adalah Fusarium spp. dan Aspergillus spp.Kata kunci : Rekalsitran, viabilitas, kadar air benih


Author(s):  
Hayati Akman

This study targeted to elucidate the effect of seed aging on germination and emergence rates with and shoot characteristics in wheat cultivars. For this purpose, different bread wheat cultivars stored for 7 years and non-stored were compared for coleoptile length, root mass, shoot mass, root length as well as germination and seedling emergence rates. Here, the evidence suggested that seed storage over a prolonged period affected root and Shoot growth, coleoptile length, seed germination, and seedling emergence rates adversely. By linking germination and emergence rates, the data presented here indicated that a reduction in emergence rate in long-term storage was higher than that in the germination rate. It was also found that there were significant variations among the wheat cultivars about investigated traits during long-term storage. However, the emergence rates of Kate A1 and Flamura 85 were not affected substantially by long-term storage. The study suggested future studies to focus on clarification of the process controlling natural seed aging as such knowledge allows clue the eventual consequences of long-term storage.


HortScience ◽  
1991 ◽  
Vol 26 (8) ◽  
pp. 1054-1057 ◽  
Author(s):  
W.J. Carpenter ◽  
G.J. Wilfret ◽  
J.A. Cornell

Gladiolus (G. grandiflorus) seed germination was light-independent, but temperature influenced the germination rate. Constant 20C promoted higher total germination (97%), fewer days (4.3) to 50% of final germination, and shorter span of days (4.8) between 10% and 90% germination than other constant temperatures, although similar results were achieved by alternating 12-h cycles of 20 to 25C. Total germination was unchanged after seed treatment for 7 days at 10 to -20C, but longer germination periods were required after treatments below -10C. Reducing seed moisture contents from 11.8% to 4.2% caused no reduction in total germination, but moisture contents below 6.6% delayed achieving 50% of final germination and extended the periods from 10% to 90% of germination. Temperature and relative humidity (RH) during storage were important in retaining seed viability, with RH having a larger effect. Smallest declines in total germination during 12 months of storage occurred at 11% and 33% RH at 15C. The statistical analysis estimated the optimum seed storage at 14C and 26% RH.


Author(s):  
Sulistyani Pancaningtyas ◽  
Teguh Iman Santoso ◽  
Sudarsianto .

Study of germination methods conduct to get information about seed viability based on germination rate, percentage of germination and vigority. Germination methods was studied to get the efficiency and effectivity of germination, easy to handle, low costs with high vigority. Sand and gunny sack methods  for germination, need extensive place  and 3-4 days germination period after planting. This research will study the alternative of germination method with soaking. This method can be accelerating  germination rate and effectively place usage without decreasing the quality of cacao seedling.The research was done at Kaliwining Experimental Station, Indonesian Coffee and Cocoa Research Institue. This research consist of two experiment was arranged based on factorial completely random design. First experiment will observed to compared germination rate and the second experiment will observed seedling quality between soaking and wet gunny sack germination method.The results showed that length of radicel on soaking method longer than wet gunny sack method. Growth of radicel started from 2 hours after soaking, moreover length of radicel at 4 hours after soaking have significant different value with gunny sack method. On 24 hours after soaking have 3,69 mm and 0,681 mm on wet gunny sack treatment. Except lengt of hipocotyl, there is not different condition between seedling that out came  from soaking and wet gunny sack method. Length of hipocotyl on 36 hours after soaking have 9,15 cm and significant different between wet gunny sack germination method that have 5,40 cm. Keywords : seed germination, soaking method, Theobroma cacao L., cocoa seedlings


Author(s):  
O. I. Asunbo

Aim: To assess the impacts of rodent pests on Cocoa (Theobroma cacao) production and productivity. Rodents belong to the mammalian order that is widely distributed globally. Their adaptive potential and capability to face changing environments even when instigated by anthropogenic activities is quite amazing. Also, their importance as vital links in food chain between species of plants and the predators thereby playing crucial roles within the ecosystem cannot be overemphasized. Rodents have been noted to cause ubiquitous damage to agricultural crops including cocoa. They can cause damage in crop fields from planting throughout harvest and storage. The damages caused by rodent pests mostly occur during the sensitive young seedling stage and just prior to harvesting. The pattern and the extent of damage by pest rodents depend upon the species, the intensity of infestation, the type and the growth stage of the crop, and the nature of the surrounding habitat. These damages caused by rodent pests could be direct through the consumption of cocoa beans or indirect through the biting, gnawing or scratching of pods which could range from slight to severe. The stern impacts caused as a result of rodent pest activities are not only felt in the quantity and quality of cocoa pods but also on the social maintenance as well as survival of those growing the crop. Despite the paucity of documented data as regards the kind, form as well as ferocity of rodent damage, it is highly expedient to re-evaluate the rodent pests’ impacts on the production of cocoa so as to update the very scarce available information whilst providing a basis for additional investigation.


2013 ◽  
Vol 46 (2) ◽  
pp. 52-57
Author(s):  
Efemena Dolor Dickens

Abstract Treculia africana is an economically important but often neglected tropical fruit tree native to many tropical countries. The main objective of this study was to evaluate the seedling emergence and seedling quality of Treculia africana seeds as influenced by storage duration and propagation media. Storage duration influenced percentage seedling emergence and seedling growth. Generally, no germination was recorded in the seeds stored beyond two weeks, irrespective of the propagation media used. There was a highly significant (P < 0.01) storage duration × propagation media interaction on all parameters measured. The highest germination percentage (87.5%) was recorded by freshly collected seeds sown in both medium sand and fine sand. A consistently higher plant height, number of leaves and leaf area was recorded in fresh seeds sown in medium sand. The effect of media was not significant. The study suggests that seed storage had a deleterious effect on the germination of the species, thus implying that storing the seeds of T. africana beyond two weeks could impede viability and recommends that T. africana seeds should be sown immediately the fruits are harvested preferably in medium sand.


Nabatia ◽  
2016 ◽  
Vol 4 (2) ◽  
pp. 109-117
Author(s):  
Tri Widiyanti ◽  
Agus Miftakhurrohmat

The aim of this study was to determine the effect of giberrelin (GA3) storage durationand treatment on the viability of cocoa (Theobroma cacao L.) seeds carried out at theLaboratory of Indonesian Center for Seedling and Plant Protection (BBPPTP) Surabayaon Mojoagung No. No. 52, Mojoagung Subdistrict, Jombang Regency in February toApril 2014. This study used a completely randomized design with factorial patterns with2 factors. The first factor is the storage time which consists of 4 stages P1 (7 days ofnatural storage of seeds), P2 (14 days of storage of natural seeds), P3 (22 days of storageof natural seeds), and P4 (29 days of storage of natural seeds). The second factor isinvigoration treatment which consists of 3 types without treatment (I0), GA3 10 ppm(I1), GA3 20 ppm (I2). The results showed a significant interaction between storage timeand invigoration treatment of plant height and germination capacity, storage durationtreatment affected the variable number of leaves, 7 days storage time (P1) produced theaverage number of leaves (3.75 strands) even though the result is the same as the storageperiod of 14 days (P2). While the invigoration treatment had no effect on the observationvariable of the number of leaves.


2018 ◽  
Vol 48 (3) ◽  
pp. 306-315
Author(s):  
Alan Mario Zuffo ◽  
Joacir Mario Zuffo Júnior ◽  
Everton Vinicius Zambiazzi ◽  
Fábio Steiner

ABSTRACT The drying process and storage may interfere in the quality of creeping signalgrass seeds [Urochloa humidicola (Rendle.) Morrone & Zuloago, syn. Brachiaria humidicola (Rendle.) Schweick.]. This study aimed to evaluate the physical, physiological and sanitary quality of creeping signalgrass cv. Llanero seeds submitted to drying surfaces and environments and stored for two periods (1 and 8 months). The experiment was arranged in a completely randomized design, in a 4 × 2 × 2 + 2 factorial arrangement, with four drying surfaces (soil, asphalt, concrete and ceramic floor), two drying environments (seeds exposed to the sun or shade), two seed storage periods (1 and 8 months) and two additional treatments with drying in paper bags under laboratory conditions (control) and stored for 1 or 8 months, with four replicates. The water content, viability by the tetrazolium test, electrical conductivity, germination rate, germination rate index and seed sanitary quality were measured. Drying at full sun impaired the physiological quality of the seeds due to the high temperatures (46.8-51.0 ºC) of the drying surface; however, reduced the incidence of pathogens. The drying of seeds in paper bags under shade results in a higher physiological quality, but the sanitary quality is compromised. However, the pathogens present in the seeds did not inhibit their viability and vigor. The storage for eight months reduces the seed quality.


1987 ◽  
Vol 11 (1) ◽  
pp. 59-65 ◽  
Author(s):  
F. T. Bonner

Abstract Effects of cone storage on seed quality were evaluated in studies conducted in Mississippi and Alabama on drying rates and storage conditions (bag vs. crate). The drying study found that: (1) Cone drying was equally rapid in indoor and outdoor covered conditions and slower in unprotected outdoor conditions; (2) seed extraction efficiency was significantly affected by all treatments with the best efficiency from cones stored outdoors unprotected; and (3) germination rate was more sensitive to treatment conditions than total germination. The second study compared burlap bagsto 20-bushel wirebound crates as storage containers for loblolly (Pinus–taeda L.) or slash (P. elliottii Engelm.) pine cones stored for varying periods. Study results reveal that (1) either bags or crates provide satisfactory storage if handled properly and (2) cone storage (4 weeks for slash,5 to 7 weeks for loblolly) enhances seed quality through apparent improved maturation. South. J. Appl. For. 11(1):59-65.


2021 ◽  
Vol 6 (1) ◽  
pp. 779-797
Author(s):  
Kyenpiya E. Deshi ◽  
Moses O. Obasi ◽  
Ngozi I. Odiaka

Abstract This study was carried out in two seasons (2010–2011 and 2012–2013) in Jos, Plateau State, Nigeria to investigate the growth and yield of potato (Solanum tuberosum L.) seed tubers as affected by storage conditions and storage duration. Five potato varieties (“Nicola,” “Bertita,” “Diamant,” “BR63-18,” and “Roslin-Ruaka”) were stored for three durations (12, 24, and 32 weeks) in three kinds of stores (room temperature store [RTS], diffused light store [DLS], and air-conditioned store [ACS]). The experimental design was a split–split plot in a randomized complete block design with the potato varieties, storage conditions, and storage durations as the main, sub, and sub–sub plots, respectively. All the parameters assessed varied with variety except the plant height. Germination and establishment were significantly higher in ACS than in RTS and DLS. RTS and DLS resulted in more aboveground stems than ACS. Storage in ACS resulted in a significantly higher plant height, leaf number, total number and yield of tubers, and yield of saleable tubers in both seasons. Seed tubers stored for 24 weeks resulted in the highest establishment count and the mean number of aboveground stems in both seasons. Aboveground stems increased from 12 to 24 weeks of storage and declined at 32 weeks. Seed storage for 12 weeks resulted in the highest total number of tubers, whereas 32 weeks had the lowest number of tubers in both seasons. Seed storage for 12 and 32 weeks resulted in the highest yield of tubers in seasons 1 and 2, respectively. In conclusion, potato varieties varied greatly in the rate of physiological aging; hence, every variety required specific storage conditions and duration for optimal growth and yield.


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