scholarly journals A Combination of UTMD-Mediated HIF-1α shRNA Transfection and TAE in the Treatment of Hepatic Cancer

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Yangying Liao ◽  
Haibo Luo ◽  
Zhizhong He ◽  
Yongpei Kuang ◽  
Peifen Chen ◽  
...  
Keyword(s):  
Survival Time ◽  
Western Blotting ◽  
Tumor Size ◽  
Statistical Difference ◽  
Antitumor Effect ◽  
Control Group ◽  
Cancer Tissue ◽  
Hepatic Cancer ◽  
Mean Survival Time ◽  
The Mean

To explore the antitumor effect of hypoxia-inducible factor-1α short hairpin RNA (HIF-1α shRNA) delivered by ultrasound targeted microbubble destruction (UTMD) and transcatheter arterial embolization (TAE) on rats with hepatic cancer. After the models of transplantation hepatoma were established, Wistar rats were randomly divided into 4 groups: Control group, UTMD group, TAE group, and UTMD+TAE group. Contrast-enhanced ultrasound (CEUS) was used to monitor tumor size on day 14 after four different treatments. Western blotting and immunohistochemistry were applied to measure the protein level of HIF-1α and VEGF in the hepatic cancer tissue. In comparison with UTMD+TAE group (21.25±10.68 days), the mean survival time was noticeably shorter in the Control group and TAE group (13.02±4.30 days and 15.03±7.32 days) (p<0.05, respectively). There was no statistical difference between UTMD+TAE group and UTMD group of the mean survival time (p>0.05). In addition, our results proved that the tumor sizes in UTMD+TAE group were obviously smaller than those in other groups (p<0.05, respectively). By CEUS, we clearly found that the tumor size was the smallest on day 14 in the UTMD+TAE group. The western blotting and immunohistochemistry results proved that the protein levels of HIF-1α and VEGF in UTMD+TAE group were obviously lower than those in TAE group and Control group on days 7 and 14 (p<0.05, respectively). However, there was no statistical difference between UTMD+TAE group and UTMD group (p>0.05). In this study we tried to explore the antitumor effect through a combination of UTMD-mediated HIF-1α shRNA transfection and TAE on rats with hepatic cancer. Our results showed that UTMD-mediated HIF-1α shRNA transfection and TAE can obviously silence HIF-1α and VEGF expression, thereby successfully inhibiting the growth of the tumor.

Der Einfluß von partiellen Hepatektomien auf die Hepatomrate nach Diäthylnitrosamin-Gaben

1970 ◽  
Vol 25 (11) ◽  
pp. 1277-1281 ◽  
Author(s):  
D. Grünthal ◽  
D. O. Hellenbroich ◽  
P. Sänger ◽  
H. Maass
Keyword(s):  
Liver Regeneration ◽  
Survival Time ◽  
Histological Examination ◽  
Control Group ◽  
Mean Survival Time ◽  
The Mean

Rats were hepatectomised and subsequently diethylnitrosamine was applied orally (2 mg DENA/kg/d). Compared with a non-treated control group the mean survival time was lowered from 225 to 158 days. In a second experiment repeated hepatectomies were performed. All these rats were killed after 40 weeks and after a total DENA dose of 351 mg/kg. By histological examination of the livers a higher rate of hepatomas was found in the hepatectomised group.Thus the proliferating processes during liver regeneration seem to favour the hepatocarcinogenesis under DENA treatment.

Determination some Immunological Aspects in Pulmonary Tuberculosis Patients in Qadisiyah Province

2018 ◽  
Vol 9 (2) ◽  
Author(s):  
Syoof Khowman Alramahy ◽  
Akram Hadi Hamza
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Statistical Difference ◽  
Positive Culture ◽  
Control Group ◽  
Tuberculosis Patients ◽  
Significant Difference ◽  
Lowenstein Jensen ◽  
The Mean ◽  
Igg Level

This study was carried out to study of some immunological aspects among the pulmonary Tuberculosis patients infected with causative agent, Mycobacterium tuberculosis. A Total of 200 sputum samples were collected from patients attending the consultant Clinic for Chest and Respiratory disease center, Diwaniya. Control group (No=15) also included. According to acid fast stain of sputum, the patients were classified as positive (No=91,45.5%) and negative (No=109,54.5, Lowenstein Jensen medium used for the cultivation of samples, on which 70% of sputum samples where positive culture for this microorganism. The grown microorganism were identified as M. tuberculosis, based on positive A.F.B, Niacin producers ,negative for catlase at 68c. The mean IgG level was l184.053±76.684 mg/100 ml in tuberculosis group compared with 1016.533 ± 44.882 mg/100ml in control group, rendering the statistical difference significant. For IgA and IgM levels, they were at mean of 315.880±38.552 mg/100 ml and 119.527±8.464 mg/100 ml in control group compared with 396.358±38.776 mg/100 ml and 134.207±11.696 mg/100 ml in patients group respectively with significant difference

scholarly journals Postoperative analgesia effects of sulfentanyl plus dexmedetomidine in patients received VATS

Pteridines ◽  
2020 ◽  
Vol 31 (1) ◽  
pp. 55-60
Author(s):  
Haoyu Jiang ◽  
Ying Zheng ◽  
Chang Liu ◽  
Ying Bao
Keyword(s):  
Statistical Difference ◽  
Control Group ◽  
Control Groups ◽  
Significant Statistical Difference ◽  
Post Surgery ◽  
Intravenous Analgesia ◽  
The Mean ◽  
Nsclc Patients ◽  
And Control ◽  
Time Point

AbstractBackground To evaluate sulfentanyl combined with dexmedetomidine hydrochloride on postoperative analgesia in patients who received video-assisted thoracic surgery (VATS) and its effects on serum norepinephrine (NE), dopamine (DA), 5-hydroxytryptamine (5-HT), and prostaglandin (PGE2).Material and Methods Ninety-nine non-small cell lung cancer (NSCLC) patients who received VATS were included in the study. All the patients received intravenous inhalation compound anesthesia. Of the 99 cases, 49 subjects (control group) received sulfentanyl for patient controlled intravenous analgesia (PICA) and other 50 cases (experiment group) received sulfentanyl combined with dexmedetomidine hydrochloride for PICA after operation of VATS. The analgesic effects of the two groups were evaluated according to Visual Analogue Scales (VAS) and the Bruggrmann Comfort Scale (BCS). The serum pain mediator of NE, DA, 5-HT, and PGE2 were examined and compared between the two groups in the first 24 h post-surgery.Results The VAS scores for the experiment group were significant lower than that of control group on the time points of 8, 16, and 24 h post-surgery (pall<0.05), and the BCS scores of the experiment group in the time points of 8, 16, and 24 h were significantly higher than that of controls (p<0.05). However, the VAS and BCS scores were not statistical differently in the time point of 1, 2, and 4 h post-surgery (pall>0.05). The mean sulfentanyl dosage was 63.01 ± 5.14 μg and 67.12 ± 6.91 μg for the experiment and control groups respectively with significant statistical difference (p<0.05). The mean analgesic pump pressing times were 4.30 ± 1.31 and 5.31 ± 1.46 for experiment and control groups respectively with significant statistical difference (p<0.05). The serum NE, DA, 5-HT, and PGE2 levels were significantly lower in the experimental group compared to that of control group in the time point of 12 h post-surgery (pall<0.05). The side effects of nausea, vomiting, delirium, rash, and hypotension atrial fibrillation were not statistically different between the two groups (pall>0.05).Conclusion Patient controlled intravenous analgesia of sulfentanyl combined with dexmedetomidine hydrochloride was effective in reducing the VAS score and serum pain mediators in NSCLC patients who received VAST.

scholarly journals Laryngeal Neuroendocrine Carcinomas: A Retrospective Study of 14 Cases

2015 ◽  
Vol 2015 ◽  
pp. 1-4 ◽  
Author(s):  
Yingying Zhu ◽  
Liming Gao ◽  
Yunxiao Meng ◽  
Wenwen Diao ◽  
Xiaoli Zhu ◽  
...  
Keyword(s):  
Survival Time ◽  
Neuroendocrine Carcinoma ◽  
Clinical Manifestations ◽  
Small Cell ◽  
Atypical Carcinoid ◽  
Small Cell Neuroendocrine Carcinoma ◽  
Typical Carcinoid ◽  
Mean Survival Time ◽  
The Mean ◽  
Neuroendocrine Carcinomas

Laryngeal neuroendocrine carcinomas (LNECs) are rare and highly heterogeneous which present a wide spectrum of pathological and clinical manifestations. Fourteen patients with histologically demonstrated LNEC were collected and analyzed retrospectively. The 14 cases were classified into 3 subtypes: typical carcinoid in 2, atypical carcinoid in 5, and small cell neuroendocrine carcinoma in 7. The mean survival time of the 14 patients in this study was 112.5 months (95% CI, 81.5–143.6). Surgeries were performed for 2 patients of typical carcinoid, and they were alive with no evidence of recurrence after 24 and 47 months of follow-ups. Patients in the atypical carcinoid group were treated with surgeries and postoperative radiotherapy. After 58.4 months of follow-ups (range: 9–144), 2 patients showed no evidence of disease and 1 was lost to follow-up after 72 months. The other 2 patients died of other unrelated diseases. In the small cell neuroendocrine carcinoma group, a combination of chemotherapy and radiotherapy was applied. The mean survival time was 79.7 months (95% CI, 37.9–121.4), and the 5-year survival rate was 53.6%. In conclusion, the clinical behaviors, treatment protocols, and prognosis are different for each subtype of LNECs.

Effect of Intravenous Iron in Experimental Traumatic Shock

1956 ◽  
Vol 186 (3) ◽  
pp. 554-556
Author(s):  
Donn L. Smith ◽  
Irvin I. Kibbey ◽  
Max E. Bierwagen ◽  
J. R. Cruse
Keyword(s):  
Heavy Metal ◽  
Survival Time ◽  
Metal Toxicity ◽  
Intravenous Iron ◽  
Traumatic Shock ◽  
Survival Times ◽  
Albino Rat ◽  
Liver Iron ◽  
Mean Survival Time ◽  
The Mean

Intravenous administration of colloidal saccharated iron oxide prior to intestinal traumatization in the albino rat resulted in a significant reduction of the mean survival time. Sodium gold thiosulfate and colloidal manganese hydroxide employed in the same manner did not significantly alter mean survival times. ACTH and cortisone did not modify the deleterious effects of iron in experimental traumatic shock. A decrease in soluble liver iron was observed when traumatization followed the injection of iron. It was concluded that the reduction of mean survival time in iron injected, traumatized animals was due to a specific action of iron and is not the result of generalized heavy metal toxicity.

scholarly journals Big End Double-Layer Stents for the Treatment of Gastric Outlet Obstruction Caused by Stomach Cancer

2019 ◽  
Vol 2019 ◽  
pp. 1-6
Author(s):  
Ding Shi ◽  
Dong Wu ◽  
Yongpan Liu ◽  
Feng Ji ◽  
Yinsu Bao
Keyword(s):  
Gastric Cancer ◽  
Survival Time ◽  
Stomach Cancer ◽  
Double Layer ◽  
Gastric Outlet Obstruction ◽  
Outlet Obstruction ◽  
Metal Stents ◽  
Mean Survival Time ◽  
Distal Gastric Cancer ◽  
The Mean

Objectives. This study is aimed at evaluating the efficacy and safety of the big end double-layer uncovered self-expanding metal stents (SEMS) for the treatment of gastric outlet obstruction (GOO) caused by distal stomach cancer.Methods. Seventy three patients receiving big end double-layer uncovered SEMS for the treatment of GOO caused by distal gastric cancer will be included in this multicenter prospective clinical trial. The main outcome measures included the functional outcome, the complications, the reinterventional rates, the average treatment charges, and the mean survival time. Monthly telephone calls were needed to assess the food intake until the patients died.Results. The technical and the clinical success rates were 98.6%. The stent obstruction caused by tumor ingrowth was observed in one patient (1.4%). The incidence of food impaction was 2.9% (2/70) and the reinterventional rate was 4.3% (3/70). However, stent migration and obstruction caused by overgrowth were not observed. No perforation and severe bleeding were observed. The median cost of endoscopic stenting and total hospitalization (including reinterventions) for the big end double-layer uncovered SEMS in this study was $2945 and $3408, respectively. The mean survival time was 212.5 days.Conclusions. The placement of big end double-layer uncovered SEMS is a safe and effective modality and has the potential to be one of the options for the treatment of GOO caused by the distal gastric cancer.

Different effects of hypothermia on two syndromes of positive acceleration

1963 ◽  
Vol 18 (2) ◽  
pp. 387-392 ◽  
Author(s):  
E. R. Stiehm
Keyword(s):  
Survival Time ◽  
Cardiac Failure ◽  
Cerebral Circulation ◽  
The Other ◽  
Mean Survival Time ◽  
Cardiac Syndrome ◽  
Positive Acceleration ◽  
The Mean

The tolerance to high positive (headward) acceleration at levels of 20–80 G was studied in normal and hypothermic rats. Normal rats have a mean survival time of 680 sec at 20 G, but this decreases to 105 sec at 30 G. Only slight decreases are noted at higher G, suggesting that 30 G is the point at which cerebral circulation is interrupted. Hypothermia at 22.5 C decreases acceleration tolerance at 20 G, but markedly increases acceleration tolerance at 30 G and above. At 40 G this effect is maximal, and the mean survival time for hypothermic rats is 252 sec, compared to 75 sec for controls, an increase of 236%. These different effects of hypothermia on acceleration tolerance suggest that there are two distinct syndromes of physiologic failure during positive acceleration. One is a “cardiac” syndrome, occurring at 20 G and below, characterized by partial maintenance of cerebral circulation until cardiac failure ensues, and the other is a “cerebral” syndrome, occurring at 30 G and above, characterized by immediate interruption of cerebral circulation and respiratory paralysis. Submitted on May 17, 1962

Relationship Between Survival Rate and Survival-Time of Rabbits, Oryctolagus-Cuniculus (L), Challenged With Myxoma Virus

1995 ◽  
Vol 43 (3) ◽  
pp. 303 ◽  
Author(s):  
I Parer
Keyword(s):  
Survival Time ◽  
Small Groups ◽  
Regression Line ◽  
Survival Rates ◽  
Myxoma Virus ◽  
Survival Times ◽  
Mean Survival Time ◽  
The Past ◽  
Survival Percentage ◽  
The Mean

The mean survival times of small groups of rabbits challenged with myxoma virus have been used to estimate survival rates and to allocate virulence grades to field strains of myxoma virus. The slope of the regression Line relating survival percentage to mean survival time in days was shown to be less steep than has been previously estimated. This overestimation of the regression slope has, in the past, resulted in most field strains of myxoma virus being allocated to the Grade III level of virulence when allocation to Grade I would have been more appropriate.

scholarly journals Evaluation of the Survival of HER2-Positive Breast Cancer Patients Receiving Herceptin Compared With Those Who Did Not Receive It

2018 ◽  
Vol 3 (2) ◽  
pp. 63
Author(s):  
Ali Akhavan ◽  
Fariba Binesh ◽  
Somaye Sadat Hoseini-Dezki ◽  
Seyed Mohammad Reza Mortazavizadeh
Keyword(s):  
Breast Cancer ◽  
Survival Time ◽  
Cancer Patients ◽  
Recurrence Time ◽  
Breast Cancer Patients ◽  
Her2 Positive ◽  
Mean Survival Time ◽  
Her2 Positive Breast Cancer ◽  
The Mean ◽  
Positive Breast Cancer

Introduction: Breast cancer is the most common type of cancer that affects women. Human epidermal growth factor receptor 2 (HER2) is a gene that plays a significant role in the development of breast cancer. The aim of this study was to compare the survival of HER2-positive breast cancer patients who received Herceptin with those who did not receive it in Yazd, Iran.Materials and Methods: Our study was an analytical cross-sectional study. Sampling was done on all patients with HER2-positive invasive ductal carcinoma (HER2 positive) who were referred to Shahid Sadoughi Hospital or Shahid Ramezanzadeh Hospital from 2003 to 2014. The data were collected using a questionnaire, which included questions on age, type of tumor, tumor grade, recurrence history, tumor size, number of lymph nodes, and whethr Hercepin was received or not received. Data were then entered into SPSS version 18 and analyzed by statistical tests.Results: The mean age of the patients was 50.78 ± 10.75 years. The mean survival time was 61.61 ± 2.93 months, and the mean recurrence time was 104.104 ± 41.3 months. The results also showed that there was no statistically significant difference between the 2 groups, despite the higher mean survival time and less recurrence time in patients receiving Herceptin compared with those who did not receive Herceptin (P>.05).Conclusions: According to the results of this study, the efficacy of Herceptin as a neoadjuvant treatment in the survival of HER2-positive breast cancer patients has not been established.

scholarly journals Evaluation of Antimalarial Potential of Extracts from Alstonia boonei and Carica papaya in Plasmodium berghei-Infected Mice

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Francis O. Atanu ◽  
Favour M. Idih ◽  
Charles O. Nwonuma ◽  
Helal F. Hetta ◽  
Salman Alamery ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Survival Time ◽  
Plasmodium Berghei ◽  
Carica Papaya ◽  
Negative Control ◽  
Control Group ◽  
Dependent Manner ◽  
Antioxidant Power ◽  
Mean Survival Time

Extracts of Alstonia boonei and Carica papaya are used in herbal medicine for the treatment of malaria. This work investigated the phytochemical, antioxidant, and antimalarial effects of hydromethanolic extracts of Alstonia boonei and Carica papaya. A four-day chemosuppressive test was conducted to assess the ability of the extracts to prevent establishment of infection. Three doses of the extracts were administered—100, 200, and 400 mg/kg bw—prior to Plasmodium berghei challenge. Change in body weight, parasitemia, packed cell volume (PCV), and mean survival time was determined. A three-day curative test was also carried out on Plasmodium berghei-infected mice to determine the effects of the plant extracts (200 mg/kg bw) on parasitemia and biochemical indices of liver and kidney functions, lipid metabolism, and oxidative stress. The study revealed that the extracts possessed phenolic compounds (34.13 ± 1.90 mg GAE/g for Alstonia boonei and 27.99 ± 1.46 mg GAE/g for Carica papaya) and flavonoids (19.47 ± 1.89 mg QE/g for Alstonia boonei and 18.24 ± 1.36 mg QE/g for Carica papaya). In vitro antioxidant activity measured as total antioxidant power, total reducing power, and DPPH radical scavenging activity showed that the extracts possessed higher antioxidant activity than the reference compounds. The outcome of the chemosuppressive test revealed that whereas Plasmodium berghei-infected mice had high parasitemia, decreased mean survival time, exhibited loss of weight, and had low PCV, treatment with the extracts reversed the effects in a concentration-dependent manner. Similarly, the curative test revealed that the extracts significantly suppressed parasitemia compared with the malaria negative control group. This was mirrored by reversal of indices of hepatic toxicity (AST, ALT, and ALP levels), nephropathy (urea and creatinine levels), oxidative stress (SOD, CAT, GPx, GSH, and lipid peroxides), and dyslipidemia (TC, HDL, and TG levels and HMG-CoA reductase activity) in infected but treated mice compared with negative control. Put together, the results of this study demonstrate that the extracts of Alstonia boonei and Carica papaya possess antimalarial properties and are able to ameliorate metabolic dysregulations that characterize Plasmodium berghei infection. The phytoconstituents in these extracts are believed to be responsible for the pharmacological activity reported in this study.

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