scholarly journals Plasma miR-22-5p, miR-132-5p, and miR-150-3p Are Associated with Acute Myocardial Infarction

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Huixian Li ◽  
Pengxiang Zhang ◽  
Fangjiang Li ◽  
Guili Yuan ◽  
Xiaoyuan Wang ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Plasma Levels ◽  
Troponin I ◽  
Early Stage ◽  
Diagnostic Value ◽  
Diagnostic Efficacy ◽  
Diagnostic Biomarkers ◽  
Circulating Micrornas ◽  
Novel Biomarkers

Circulating microRNAs (miRNAs) are potential biomarkers for cardiovascular diseases. Our study aimed to determine whether miR-22-5p, miR-132-5p, and miR-150-3p represent novel biomarkers for acute myocardial infarction (AMI). Plasma samples were isolated from 35 AMI patients and 55 matched controls. Total RNA was extracted, and quantitative real-time PCR and ELISA were performed to investigate the expressions of miRNAs and cardiac troponin I (cTnI), respectively. We found that plasma levels of miR-22-5p and miR-150-3p were significantly higher during the early stage of AMI and their expression levels peaked earlier than cTnI. Conversely, circulating miR-132-5p was sustained at a low level during the early phase of AMI. All three circulating miRNAs were correlated with plasma cTnI levels. A receiver operating characteristic (ROC) analysis suggested that each single miRNA had considerable diagnostic efficacy for AMI. Moreover, combining the three miRNAs improved their diagnostic efficacy. Furthermore, neither heparin nor medications for coronary heart disease (CHD) affected plasma levels of miR-22-5p and miR-132-5p, but circulating miR-150-3p was downregulated by medications for CHD. We concluded that plasma miR-22-5p, miR-132-5p, and miR-150-3p may serve as candidate diagnostic biomarkers for early diagnosis of AMI. Moreover, a panel consisting of these three miRNAs may achieve a higher diagnostic value.

scholarly journals Circulating MicroRNAs are Promising Novel Biomarkers of Acute Myocardial Infarction

2011 ◽  
Vol 50 (17) ◽  
pp. 1789-1795 ◽  
Author(s):  
Rongrong Wang ◽  
Ning Li ◽  
Yinhui Zhang ◽  
Yuqin Ran ◽  
Jielin Pu
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Circulating Micrornas ◽  
Novel Biomarkers

scholarly journals Circulating MiR-19b-3p, MiR-134-5p and MiR-186-5p are Promising Novel Biomarkers for Early Diagnosis of Acute Myocardial Infarction

2016 ◽  
Vol 38 (3) ◽  
pp. 1015-1029 ◽  
Author(s):  
Ke-Jing Wang ◽  
Xin Zhao ◽  
Yu-Zhou Liu ◽  
Qiu-Tang Zeng ◽  
Xiao-Bo Mao ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Troponin I ◽  
Early Stage ◽  
Diagnostic Efficiency ◽  
Plasma Samples ◽  
Circulating Mirnas ◽  
Peak Expression ◽  
And Gender ◽  
The Impact

Background/Aims: Recent studies have shown that circulating microRNAs (miRNAs) are emerging as promising biomarkers for cardiovascular diseases. This study aimed to determine whether miR-19b-3p, miR-134-5p and miR-186-5p can be used as novel indicators for acute myocardial infarction (AMI). Methods: To investigate the kinetic expression of the three selected miRNAs, we enrolled 18 patients with AMI and 20 matched controls. Plasma samples were collected from each participant, and total RNA was extracted. Quantitative real-time PCR and ELISA assays were used to investigate the expression of circulating miRNAs and cardiac troponin I (cTnI), respectively. Plasma samples from another age- and gender-matched cohort were collected to investigate the impact of medications for AMI on the expression of the selected miRNAs. Results: Levels of plasma miR-19b-3p, miR-134-5p and miR-186-5p were significantly increased in early stage of AMI. Plasma miR-19b-3p and miR-134-5p levels reached peak expression immediately after admission (T0), whereas miR-186-5p achieved peak expression at 4 h after T0. All of these times were earlier than the peak for cTnI (8 h after T0). In addition, all three miRNAs were positively correlated with cTnI. Receiver Operating Characteristic (ROC) analysis indicated that each single miRNA showed considerable diagnostic efficiency for predicting AMI. Furthermore, combining all three miRNAs in a panel increased the efficiency of distinguishing between patients with AMI and controls. Moreover, we found that heparin and medications for AMI did not impact the expression of these circulating miRNAs. Conclusion: Circulating miR-19b-3p, miR-134-5p and miR-186-5p could be considered promising novel diagnostic biomarkers for the early phase of AMI.

scholarly journals Circulating microRNAs: A Potential Role in Diagnosis and Prognosis of Acute Myocardial Infarction

2013 ◽  
Vol 35 ◽  
pp. 561-566 ◽  
Author(s):  
Ali Sheikh Md Sayed ◽  
Ke Xia ◽  
Tian-Lun Yang ◽  
Jun Peng
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Correct Diagnosis ◽  
High Sensitivity ◽  
Stable Form ◽  
Circulating Mirnas ◽  
Circulating Micrornas ◽  
Diagnosis And Prognosis ◽  
Novel Biomarkers ◽  
Rule Out

Rapid and correct diagnosis of acute myocardial infarction (AMI) plays a crucial role in saving patients' life. Although some biomarkers (such as cardiac troponin and creatine kinase) are available for AMI diagnosis so far, there is still a clinical need for novel biomarkers, which can reliably rule in or rule out AMI immediately on admission. Circulating microRNAs (miRNAs) are a potential choice for novel biomarkers in AMI diagnosis and prognosis with high sensitivity and specificity. Circulating microRNAs are endogenous miRNAs that are detectable in whole blood, serum, or plasma in a highly stable form. Until now, around 20 circulating miRNAs were reported to be closely associated with AMI. In this minireview, we summarized recent available data on the correlation between circulating miRNAs and AMI. Some miRNAs, such as miR-208, miR-499, miR-133, and miR-1, were given special attention, since they may have a potential prospect in diagnosis and prognosis of AMI.

scholarly journals Circulating endothelial microparticles and miR-92a in acute myocardial infarction

2017 ◽  
Vol 37 (2) ◽  
Author(s):  
Yuchen Zhang ◽  
Junjun Cheng ◽  
Fang Chen ◽  
Changyan Wu ◽  
Junmeng Zhang ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Endothelial Dysfunction ◽  
Operating Characteristic ◽  
Troponin I ◽  
Diagnostic Value ◽  
Diagnostic Parameter ◽  
Reverse Transcription Quantitative Pcr ◽  
Artery Disease ◽  
Stable Cad

Microparticles (MPs) and miRNAs have been shown to play important roles in coronary artery disease (CAD) by monitoring endothelial dysfunction. The present study aims to investigate the diagnostic value of endothelial MPs (EMPs) and miRNAs (miR-92a or miR-23a) as biomarkers in distinguishing patients with acute myocardial infarction (AMI) from those with CAD. Plasma samples from 37 patients with AMI, 42 patients with stable CAD (SCAD), and 35 healthy adults were collected for investigation in the present study. The numbers of CD31+/CD42b− MPs, CD31+/CD42b+ MPs, and CD31−/CD42b− MPs were measured by flow cytometry and the levels of miR-92a and miR-23a were analyzed using reverse transcription-quantitative PCR. Moreover, cardiac troponin I (cTnI) expression was detected by ELISA to serve as a routine diagnostic parameter. The number of CD31+/CD42b− was higher in AMI group than those in SCAD and healthy groups. Besides, the expression of miR-92a was higher in AMI group compared with two other groups. Furthermore, evidence showed that there was a positive correlation between the levels of CD31+/CD42b− MPs and miR-92a. Finally, the receiver operating characteristic (ROC) curve revealed that the area value under the curve of CD31+/CD42b− MPs, miR-92a and cTnI was 0.893, 0.888, and 0.912 respectively. CD31+/CD42b− MPs and miR-92a might have great potential to provide diagnostic value for AMI and could probably regulate the endothelial dysfunction in AMI patients.

Butyrylcholinesterase as an additional marker in the diagnostic network of acute myocardial infarction

2016 ◽  
Vol 40 (2) ◽  
Author(s):  
Ramazan Kocabaş ◽  
Ali Kemal Erenler ◽  
Mücahit Yetim ◽  
Tolga Doğan ◽  
Hacı Kemal Erdemli
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Healthy Subjects ◽  
Early Stage ◽  
Characteristic Curve ◽  
Myocardial Damage ◽  
Diagnostic Value ◽  
Coronary Syndrome ◽  
Additional Marker ◽  
Low Sensitivity

AbstractAcute coronary syndrome defines a broad spectrum of complaints from angina to irreversible myocardial damage. There is an ongoing need for a biomarker to predict and diagnose acute myocardial infarction (AMI) in the early stage. In this study, our aim was to reveal early diagnostic value of butyrylcholinesterase (BChE) in discrimination of healthy subjects and patients with AMI.Eighty-five patients admitted to our hospital due to AMI and 45 healthy subjects were involved in the study. Patients and controls were compared according to BChE, lipid profiles and biochemical parameters.The serum BChE activity was significantly lower in patients with AMI than in the controls (p<0.001). After correlation analysis, while a negative correlation was determined between the serum BChE concentrations and AMI presence (r=–0.363, p<0.001); a positive correlation was determined between the serum BChE and cholesterol (r=0.443, p<0.001), HDL (r=0.243, p=0.006) and LDL (r=0.369, p<0.001) levels. The data indicate that BChE is associated with AMI and a subsequent receiver operating characteristic curve (ROC) analysis revealed that BChE, as an independent indicator, may differentiate AMI patients from controls. A cut-off point set at ≤7.15 kIU/L, BChE showed a sensitivity of 51.2% and a specificity of 84.4% (AUC=0.719, p<0.001).Low BChE level was significantly associated with AMI when compared to healthy subjects. Even though it has low sensitivity, plasma levels of BChE might represent an additional marker in the diagnostic network of AMI.

EARLY SPONTANEOUS FIBRINOLYSIS IS RELATED TO THE EXTENSION OF ACUTE MYOCARDIAL INFARCTION

1987 ◽  
Author(s):  
M Hanss ◽  
D Rousson ◽  
P Touboul ◽  
M Dechavanne
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Plasma Levels ◽  
Early Stage ◽  
Venous Blood ◽  
Peak Level ◽  
Enzyme Linked Immunosorbent Assay ◽  
Significant Linear Correlation ◽  
Coefficient Corrélation ◽  
Two Parameters

The relative importance of spontaneous fibrinolysis was studied during the early stage of acute myocardial infarction (AMI). Ten consecutive male patients (52.1 ± 5.7 years old) with AMI and without haemodynamic or rythmic complications were selected. Blood samples were obtained less than 5 hours (3.4 ± 0.8 hours) after the onset of symptoms. Enzyme linked immunosorbent assay procedures were performed to quantify the D-dimer antigen (Dd-Ag) and tissue plasminogen activator antigen (tPA-Ag) plasma levels. The creatine phosphokinase (CPK) peak level was measured in serum as an index of the AMI extension. Mean ± S.D. (range) levels were respectively 368 ± 342 (118-1100) μg/1 for Dd-Ag, 12.9 ± 9.4 (4.5 - 29.1) μg/1 for tPA-Ag and 1117 ± 856 (256-2800) U/l for CPK. Coefficient correlation (r) between these parameters are given in the table.A significant linear correlation was observed between tPA-Ag and Dd-Ag. Moreover, plasma levels of these two parameters were inversely correlated to the logarithm of the CPK peak level. Thus abnormaly high tPA-Ag levels are detected in plasma from peripheric venous blood when likely marked fibrin lysis occurs and if AMI size is limited.These data suggest that impaired fibrinolysis is probably involved in the .progression of coronary occlusion during the early stage of AMI.

scholarly journals A Four-MicroRNA Panel in Peripheral Blood Identified as an Early Biomarker to Diagnose Acute Myocardial Infarction

2021 ◽  
Vol 12 ◽  
Author(s):  
Liang Chen ◽  
Jie Bai ◽  
Jun Liu ◽  
Huihe Lu ◽  
Koulong Zheng
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Peripheral Blood ◽  
Troponin I ◽  
Characteristic Curve ◽  
Disease Onset ◽  
Diagnostic Value ◽  
Control Group ◽  
Expression Levels ◽  
Creatine Kinase Mb

Objective: This study aimed to evaluate suitable circulating microRNAs (miRNAs) as diagnostic biomarkers of acute myocardial infarction (AMI).Methods: Patients with AMI were enrolled as study participants. All patients with AMI coming from the Second Affiliated Hospital of Nantong University between October 1, 2017 and May 31, 2019 were screened. At the same time, 80 patients with coronary angiographic stenosis &lt;50% during the same period were selected as the control group. Peripheral blood samples were collected at different time points (0, 6, 12, and 24 h after disease onset) to detect the expression of a previously identified promising four-microRNA panel. The expression levels of miRNAs were tested by real-time polymerase chain reaction (RT-PCR), and the receiver operating characteristic curve (ROC) was used to analyze the diagnostic value of circulating miRNAs.Results: Based on the inclusion and exclusion criteria, 80 patients with AMI and 80 controls were enrolled in this study. The expression of circulating miR-1291, miR-217, miR-455-3p, and miR-566 was significantly downregulated in patients with AMI compared with controls. The area under the ROC curve (AUC) of circulating miR-1291, miR-217, miR-455-3p, and miR-566 were 0.82, 0.79, 0.82, and 0.83, respectively. The AUC of these four miRNAs was 0.87 with 83% sensitivity and 87% specificity. The expression peaks of these four miRNAs occurred earlier than those of cardiac troponin I (cTnI) and creatine kinase-MB (CK-MB). Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the targets of these four miRNAs were significantly enriched in several signaling pathways associated with AMI progression.Conclusion: Circulating miR-1291, miR-217, miR-455-3p, and miR-566 expression levels were significantly lower in patients with AMI; and combined, this panel of four miRNAs acted as a novel and potential early diagnostic biomarker of AMI.

Conventional and novel diagnostic biomarkers of acute myocardial infarction: a promising role for circulating microRNAs

Biomarkers ◽  
2013 ◽  
Vol 18 (7) ◽  
pp. 547-558 ◽  
Author(s):  
Rina Recchioni ◽  
Fiorella Marcheselli ◽  
Fabiola Olivieri ◽  
Stefano Ricci ◽  
Antonio Domenico Procopio ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Diagnostic Biomarkers ◽  
Circulating Micrornas
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