scholarly journals siRNA Design to Silence the 3′UTR Region of Zika Virus

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
María Perez-Mendez ◽  
Paola Zárate-Segura ◽  
Juan Salas-Benito ◽  
Fernando Bastida-González
Keyword(s):  
Public Health ◽  
Blood Transfusion ◽  
Aedes Aegypti ◽  
Cell Line ◽  
Birth Defects ◽  
Zika Virus ◽  
In Silico ◽  
Small Interfering Rnas ◽  
Medical Problems ◽  
Sirna Design

The disease caused by the Zika virus (ZIKV) has positioned itself as one of the main public health problems in Mexico. One of the main reasons is it causes microcephaly and other birth defects. The transmission of ZIKV is through Aedes aegypti and Ae. albopictus mosquitoes, which are found in a larger space of the national territory. In addition, it can also be transmitted via blood transfusion, sexual relations, and maternal-fetal route. So far, there are no vaccines or specific treatments to deal with this infection. Currently, some new therapeutics such as small interfering RNAs (siRNAs) are able to regulate or suppress transcription in viruses. Therefore, in this project, an in silico siRNA was designed for the 3′UTR region of ZIKV via bioinformatics tools. The designed siRNA was synthesized and transfected into the C6/36 cell line, previously infected with ZIKV in order to assess the ability of the siRNA to inhibit viral replication. The designed siRNA was able to inhibit significantly (p<0.05) ZIKV replication; this siRNA could be considered a potential therapeutic towards the disease that causes ZIKV and the medical problems generated.

scholarly journals Zika virus: A newly emergent vector-borne public health threat in the Americas

2016 ◽  
Author(s):  
Sujit Pujhari ◽  
Jason L. Rasgon
Keyword(s):  
Public Health ◽  
Birth Defects ◽  
Zika Virus ◽  
Human Population ◽  
Focused Attention ◽  
New Disease ◽  
Conspiracy Theories ◽  
Transmission Cycle ◽  
Public Health Threat ◽  
Vector Borne

Zika virus is a newly emergent mosquito-borne flavivirus. Once almost ignored epidemiologically, recent major outbreaks and links to neurological birth defects have focused attention on this neglected pathogen. We review the discovery, biology and symptomatology of Zika virus, what is known and not known about the mosquitoes that transmit the virus, conspiracy theories currently hampering control efforts, and potential avenues of Zika control. It is likely that Zika virus is here to stay in the Americas, so a thorough understanding of the complete epidemiological transmission cycle and potential effects on the human population will be critical for managing this new disease in the coming years.

scholarly journals Subgenomic flavivirus RNA binds the mosquito DEAD/H-box helicase ME31B and determines Zika virus transmission by Aedes aegypti

2019 ◽  
Vol 116 (38) ◽  
pp. 19136-19144 ◽  
Author(s):  
Giel P. Göertz ◽  
Joyce W. M. van Bree ◽  
Anwar Hiralal ◽  
Bas M. Fernhout ◽  
Carmen Steffens ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Zika Virus ◽  
Affinity Purification ◽  
Mechanistic Model ◽  
Virus Transmission ◽  
Protein Translation ◽  
Mass Spectrometry Analysis ◽  
Small Interfering Rnas ◽  
Spectrometry Analysis ◽  
Mosquito Infection

Zika virus (ZIKV) is an arthropod-borne flavivirus predominantly transmitted by Aedes aegypti mosquitoes and poses a global human health threat. All flaviviruses, including those that exclusively replicate in mosquitoes, produce a highly abundant, noncoding subgenomic flavivirus RNA (sfRNA) in infected cells, which implies an important function of sfRNA during mosquito infection. Currently, the role of sfRNA in flavivirus transmission by mosquitoes is not well understood. Here, we demonstrate that an sfRNA-deficient ZIKV (ZIKVΔSF1) replicates similar to wild-type ZIKV in mosquito cell culture but is severely attenuated in transmission by Ae. aegypti after an infectious blood meal, with 5% saliva-positive mosquitoes for ZIKVΔSF1 vs. 31% for ZIKV. Furthermore, viral titers in the mosquito saliva were lower for ZIKVΔSF1 as compared to ZIKV. Comparison of mosquito infection via infectious blood meals and intrathoracic injections showed that sfRNA is important for ZIKV to overcome the mosquito midgut barrier and to promote virus accumulation in the saliva. Next-generation sequencing of infected mosquitoes showed that viral small-interfering RNAs were elevated upon ZIKVΔSF1 as compared to ZIKV infection. RNA-affinity purification followed by mass spectrometry analysis uncovered that sfRNA specifically interacts with a specific set of Ae. aegypti proteins that are normally associated with RNA turnover and protein translation. The DEAD/H-box helicase ME31B showed the highest affinity for sfRNA and displayed antiviral activity against ZIKV in Ae. aegypti cells. Based on these results, we present a mechanistic model in which sfRNA sequesters ME31B to promote flavivirus replication and virion production to facilitate transmission by mosquitoes.

scholarly journals Zika virus: A newly emergent vector-borne public health threat in the Americas

2016 ◽  
Author(s):  
Sujit Pujhari ◽  
Jason L. Rasgon
Keyword(s):  
Public Health ◽  
Birth Defects ◽  
Zika Virus ◽  
Human Population ◽  
Focused Attention ◽  
New Disease ◽  
Conspiracy Theories ◽  
Transmission Cycle ◽  
Public Health Threat ◽  
Vector Borne

Zika virus is a newly emergent mosquito-borne flavivirus. Once almost ignored epidemiologically, recent major outbreaks and links to neurological birth defects have focused attention on this neglected pathogen. We review the discovery, biology and symptomatology of Zika virus, what is known and not known about the mosquitoes that transmit the virus, conspiracy theories currently hampering control efforts, and potential avenues of Zika control. It is likely that Zika virus is here to stay in the Americas, so a thorough understanding of the complete epidemiological transmission cycle and potential effects on the human population will be critical for managing this new disease in the coming years.

scholarly journals Chemometric modeling of larvicidal activity of plant derived compounds against zika virus vectorAedes aegypti: application of ETA indices

RSC Advances ◽  
2018 ◽  
Vol 8 (9) ◽  
pp. 4662-4670 ◽  
Author(s):  
Priyanka De ◽  
Rahul B. Aher ◽  
Kunal Roy
Keyword(s):  
Public Health ◽  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Zika Virus ◽  
Qsar Model ◽  
Health Concerns

Dengue, zika and chikungunya have severe public health concerns in several countries. We have developed here a QSAR model for larvicidal activity of plant derived compounds against the vectorAedes aegypti.

scholarly journals GESTÃO DOS PNEUS INSERVÍVEIS E SUA CONTRIBUIÇÃO PARA O PROGRAMA DE PREVENÇÃO À DENGUE, FEBRE CHIKUGUNYA E ZIKA VÍRUS NO MUNICÍPIO DE CHAPECÓ (SC)

2018 ◽  
Vol 7 (1) ◽  
pp. 129-143
Author(s):  
Junir Antônio Lutinski ◽  
Suiane Oliveira De Quadros ◽  
Jessica Tiburski ◽  
Cleia De Fátima Bedim
Keyword(s):  
Public Health ◽  
Health Promotion ◽  
Aedes Aegypti ◽  
Zika Virus ◽  
Health Surveillance ◽  
Chikungunya Fever ◽  
Breeding Sites ◽  
Waste Tires ◽  
Urban Solid Waste ◽  
The Relationship

O potencial dos pneus inservíveis como criadouros para o mosquito Aedes aegypti, vetor da Dengue, febre Chikungunya e Zika vírus, é amplamente conhecido. Este estudo teve como objetivos descrever a gestão dos pneus inservíveis no município de Chapecó e avaliar relação entre o número de pneus destinados à Reciclanip e o número de focos de A. aegypti registrados em pneus. A coleta de dados foi realizada no período de maio a agosto de 2016 e teve como base o período de 2010 a 2015. Dados foram obtidos no sitio eletrônico da DIVE/SC e junto ao setor de Vigilância em Saúde Ambiental do município de Chapecó. A quantidade de pneus inservíveis recebidos pelo ecoponto triplicou no período avaliado. A correlação entre a gestão ambientalmente correta dos pneus inservíveis e o número de focos de A. aegypti foi negativa (r = -0,79) e significativa (p0,05). Os resultados encontrados permitem inferir que a gestão ambientalmente correta dos pneus inservíveis contribui positivamente para a promoção da saúde pública, na prevenção da Dengue, febre Chikungunya e Zika vírus.Palavras-chave: Gestão de resíduos. Logística reserva. Promoção da saúde. Resíduos sólidos urbanos. ABSTRACT: The potential of waste tires as breeding sites for the Aedes aegypti mosquito, Dengue, Chikungunya fever and Zika virus vector, is widely known. The aim of this study was to describe the management of waste tires in the municipality of Chapecó and to evaluate the relationship between the number of tires destined for Reciclanip and the number of A. aegypti outbreaks recorded in tires. Data collection was performed from May to August 2016 and was based on the period from 2010 to 2015. Data were obtained from the DIVE/SC website and from the Environmental Health Surveillance sector of Chapecó municipality. The amount of waste tires received by the ecopoint tripled in the evaluated period. The correlation between the environmentally correct management of the waste tires and the number of A. aegypti outbreaks was negative (r = -0.79) and significant (p 0.05). The results found allow to infer that the environmentally correct management of waste tires contributes positively to the promotion of public health in the prevention of Dengue, Chikungunya fever and Zika virus.Keywords: Waste management. Reserve logistics. Health promotion. Urban solid waste.

scholarly journals Zika virus in Brazil: an overview of public health policies

2020 ◽  
Vol 12 (10) ◽  
pp. e4597
Author(s):  
Heverton Valentim Colaço Da Silva ◽  
Almerinda Agrelli ◽  
Ana Sofia Lima Estevão De Oliveira ◽  
Ronald Rodrigues De Moura ◽  
Sergio Crovella ◽  
...  
Keyword(s):  
Public Health ◽  
Aedes Aegypti ◽  
Zika Virus ◽  
Clinical Symptoms ◽  
The State ◽  
Health Policies ◽  
Breeding Sites ◽  
Public Health Policies ◽  
Mosquito Breeding ◽  
Primary Focus

Objective: The purpose of this review was to discuss the effectiveness of public health policies in controlling Zika virus (ZIKV) in Brazil from 2015 to 2019 with an emphasis in the state of Pernambuco. Literature Review: Zika virus is an arbovirus primarily transmitted to humans by Aedes aegypti mosquitoes. Since 2015, the control of Aedes aegypti proliferation and diffusion has been a global subject of discussion due to its role in ZIKV transmission and the emergence of serious clinical symptoms resulting from this infection. In Brazil, the public policies established to control ZIKV outbreak were mostly based on vector control. Final Considerations: The coping model to manage the transmission vector of ZIKV is not showing to be effective.  Furthermore, until the social factors that favor the development and maintenance of mosquito breeding sites are eradicated, Brazil will continue to be susceptible to new outbreaks of mosquito-driven arboviruses. Here, we discussed the effectiveness of public health policies for the control of ZIKV in Brazil from 2015 to 2019, with a primary focus on the state of Pernambuco (PE).

scholarly journals Molecular Responses to the Zika Virus in Mosquitoes

Pathogens ◽  
2018 ◽  
Vol 7 (2) ◽  
pp. 49 ◽  
Author(s):  
Catalina Alfonso-Parra ◽  
Frank Avila
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Birth Defects ◽  
Molecular Interactions ◽  
Zika Virus ◽  
Neurological Complications ◽  
Mosquito Vector ◽  
Zikv Infection ◽  
Molecular Responses ◽  
The Pacific

The Zika virus (ZIKV), originally discovered in 1947, did not become a major concern until the virus swept across the Pacific and into the Americas in the last decade, bringing with it news of neurological complications and birth defects in ZIKV affected areas. This prompted researchers to dissect the molecular interactions between ZIKV and the mosquito vector in an attempt to better understand not only the changes that occur upon infection, but to also identify molecules that may potentially enhance or suppress a mosquito’s ability to become infected and/or transmit the virus. Here, we review what is currently known regarding ZIKV-mosquito molecular interactions, focusing on ZIKV infection of Aedes aegypti and Aedes albopictus, the primary species implicated in transmitting ZIKV during the recent outbreaks.

scholarly journals Zika virus (ZIKV) disease: past, present and future

2018 ◽  
Vol 8 (6-s) ◽  
pp. 320-327 ◽  
Author(s):  
Sweety Khatri ◽  
Chandni Dhanoriya ◽  
Deepak Kumar Jain
Keyword(s):  
Public Health ◽  
Aedes Aegypti ◽  
Zika Virus ◽  
Mosquito Control ◽  
Rna Virus ◽  
Clinical Manifestations ◽  
Autoimmune Disorder ◽  
Public Health Authority ◽  
Health Crisis ◽  
The World

The mosquito-borne arbovirus Zika virus (positive-stranded RNA virus, ZIKV, Flavivirus, Flaviviridae), has caused an outbreak imposing by its extent and quick spread. This became the focus of a current pandemic and public health crisis all around the world because of the incessant geographic growth of both the virus and its mosquito vectors; it is often misdiagnosed with other disease like yellow fever, west nile, dengue and chikungunya because of same clinical manifestation. After unprecedented huge scale outbreak of ZIKV in Pacific, Micronesian island of Yap in 2007, though ZIKV infections are in general sporadic cases or causing mild self-limiting illness, harsh symptoms have been explained including neurological disorders, autoimmune disorder, fetal anomalies, impaired central nervous system of the fetus, microcephaly in newborns, meningoencephalitis, myelitis and Guillain Barre´Syndrome supposed to be linked with ZIKV. The virus is transmitted mainly by a mosquito Aedes aegypti, whereas, other routes of viral broadcast includes monkey bite, coitus and body fluids such as semen, blood and saliva which needs further corroboration. The relationship between these conditions with ZIKV infection is still not established and is under assessment. Till now there is no vaccine or specific antiviral against ZIKV, therefore the public health authority focuses on preventing infection, mainly in pregnant women and virus transmitted area. WHO and other health officials are working on the expansion of new projects and mosquito control techniques to manage up with infection as there is very fewer literature present on the pathogenesis of the ZIKV to help understand the clinical disease spectrum and target treatments to decrease or stop infection. The future status of ZIKV dispersal to other parts of the world is still unknown. The present review emphasizes various features of ZIKV and its history, epidemiology, transmission, clinical manifestations, progress  and advances in developing effective diagnostics, vaccines and drugs/therapeutics along with accepting suitable avoidance and control strategies to undertake this deadly emerging disease. Keywords: Zika virus, Flavivirus, Aedes aegypti, Pregnancy, Transmission, Microcephaly, Africa

Brazil's Zika fight will focus on mosquito eradication

2016 ◽  
Keyword(s):  
Public Health ◽  
Aedes Aegypti ◽  
Zika Virus ◽  
Public Health Emergency ◽  
Health Minister ◽  
Research Cooperation ◽  
Content Type ◽  
International Public Health ◽  
Recent Statement ◽  
Sanitation Infrastructure

Significance Health Minister Marcelo Castro's recent statement that Brazil is "badly losing the battle against the mosquito", though widely criticised, has proved to be politically significant. Questions have been raised as to how Brazil is currently combating the Aedes aegypti mosquito and the spread of the Zika virus. Impacts Shortcomings in health and sanitation infrastructure in poor regions may mar anti-Zika efforts. The outbreak will renew debate in other areas, such as legalisation of abortion. The declaration of Zika as an international public health emergency may boost global research cooperation.

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