scholarly journals Traditional Chinese Medicine Da-Cheng-Qi-Tang Ameliorates Impaired Gastrointestinal Motility and Intestinal Inflammatory Response in a Mouse Model of Postoperative Ileus

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Chunqiu Chen ◽  
Min Li ◽  
Xiaohong Liu ◽  
Jianwei Fan ◽  
Hong Zhang ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Small Bowel ◽  
Traditional Chinese Medicine ◽  
Inflammatory Response ◽  
Mouse Model ◽  
Inflammatory Cytokines ◽  
High Throughput ◽  
Immunohistochemical Staining ◽  
Postoperative Ileus ◽  
Gastrointestinal Dysfunction

This study was to explore the therapeutic effect and mechanism of the traditional Chinese medicine with the formula Da-Cheng-Qi-Tang (T-DCQT) and a modified Da-Cheng-Qi-Tang (M-DCQT) in a postoperative ileus (POI) mouse model. POI was induced via small bowel manipulation, and T-DCQT or M-DCQT was given by enema. The intestinal motility was measured with a charcoal mixture gavage. The intestinal tissues were collected for further studies by histopathological, qPCR, immunohistochemical staining, and Western blotting. Levels of inflammatory cytokines in blood were determined using a high-throughput liquid chip. We found that gastrointestinal dysfunction was alleviated after administration of either a T-DCQT or M-DCQT enema. Increased expression of NF-κB, p38 MAPK, and TLR4 in the intestinal tissues of POI mice were reversed following treatment. IL-1α, IL-6, MIP-1β, and IL-17 levels were significantly reduced at 24 h and 48 h following treatment, while the MCP-1 level was only observed to be reduced at 24 h after the treatment. Furthermore, compared with the T-DCQT effect, M-DCQT treatment was more effective in alleviating the increased IL-6, MIP-1β, and IL-1α levels. So, we draw a conclusion that T-DCQT or M-DCOT could ameliorate the POI-associated inflammatory response and improve GI motility in a POI mouse model.

scholarly journals Traditional Chinese medicine Da-Cheng-Qi-Tang ameliorates impaired gastrointestinal motility and intestinal inflammatory response in a mouse model of postoperative ileus

2020 ◽  
Author(s):  
Chunqiu Chen ◽  
Min Li ◽  
Xiaohong Liu ◽  
Jianwei Fan ◽  
Hong Zhang ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Inflammatory Response ◽  
Mouse Model ◽  
P38 Mapk ◽  
Therapeutic Effect ◽  
Inflammatory Cytokines ◽  
Postoperative Ileus ◽  
Control Group ◽  
Signal Pathways ◽  
Gi Motility

Abstract Background Chinese medicine decoction Da-Cheng-Qi-Tang (DCQT) is effective for treating gastrointestinal (GI) disorders, including postoperative ileus (POI); however, the mechanism by which DCQT improves intestinal motility of POI remains unknown. Purpose The aim of this study is to explore the therapeutic effect and mechanism of the decoction with the traditional formula DCQT (T-DCQT) and a modified DCQT (M-DCQT) in an experimental POI mouse model. Methods Mice were divided into 5 experimental groups with 16 mice per group as follows: (1) control group; (2) sham group; (3) POI group; (4) T-DCQT group and (5) M-DCQT group. Each group was subdivided into 2 groups in which the therapeutic effect was examined at 24h and 48h after operation. POI was induced by classic intestinal manipulation operation and the gastrointestinal(GI) motility was measured with a charcoal marking mixture gavage. The intestinal tissues were collected for evaluation of the histopathological alteration, and analysis of the expression of inflammatory signal pathways, as NF-κB, p38 MAPK, and TLR4 by qPCR, immunohistochemical staining and western-blotting, respectively. Plasma inflammatory cytokines were determined using a high-throughput liquid chip. All analyses were performed with samples collected 24 and/or 48h after operation. Results GI transit was significantly reduced in mice with POI and this dysfunction was alleviated after administration of either T-DCQT or M-DCQT enema. When compared to controls, the pathological changes in the ileum mucosal of POI mice were significantly improved, and the increased expression of NF-κB, p38 MAPK, and TLR4 in the intestinal tissues were reversed, following T- DCQT or M-DCQT treatment. Plasma inflammatory cytokines, such as IL-1α, IL-6, MCP-1, MIP-1β and IL-17 levels were significantly highly expressed in POI group, and most of them, as IL-1α, IL-6, MIP-1β and IL-17 were significantly reduced after T-DCQT or M-DCQT treatment. Conclusion The current study indicated that administration of T-DCQT or M-DCOT could ameliorate the POI-associated inflammatory response and improve GI motility, by controlling inflammatory cytokines release and modulating the expression of some inflammatory signal pathways in the POI mouse model.

scholarly journals Nigella A ameliorates inflammation and intestinal flora imbalance in DSS induced colitis mice

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Xingjiang Hu ◽  
Nana Xu ◽  
Xi Yang ◽  
Xi Hu ◽  
Yunliang Zheng ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Inflammatory Response ◽  
Therapeutic Effect ◽  
Antioxidant Activities ◽  
Intestinal Flora ◽  
Signs And Symptoms ◽  
Microbial Composition ◽  
Bacterial Genus

Abstract Nigella A, also named Sieboldianoside A, has been extracted from many kinds of Traditional Chinese Medicine (TCM), such as Nigella glandulifera, Stauntonia chinensis DC., and the leaves of Acanthopanax sieboldianus. Nigella A exhibited potential analgesic, anti-inflammatory, anti-tumor, and antioxidant activities. However, whether Nigella A could treat ulcerative colitis (UC) is still unknown. As saponins always be regarded as the kinds of ingredients that could regulate immunity and intestinal flora. This research aimed to investigate the therapeutic effect of Nigella A on UC and explore its effect on intestinal flora. We noted that Nigella A and Sulfasalazine (SASP) could significantly improve the signs and symptoms, alleviate colonic pathological injury in DSS-induced mice. The changing of many specific bacterial genus such as Lactobacillus, Porphyromonadaceae, Bacteroides and Escherichia might closely related to the recovery of intestinal inflammatory response. This study initially confirmed the therapeutic effect of Nigella A and SASP on DSS-induced colitis by improving the diversity of intestinal microbial composition. Nigella A has the potential to be developed for the treatment of UC and other disorders related to the imbalance of intestinal flora.

scholarly journals Effect of Rhizoma Paridis saponin on the pain behavior in a mouse model of cancer pain

RSC Advances ◽  
2018 ◽  
Vol 8 (31) ◽  
pp. 17060-17072 ◽  
Author(s):  
Genbei Wang ◽  
Yuanxue Liu ◽  
Yu Wang ◽  
Wenyuan Gao
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Mouse Model ◽  
Cancer Pain ◽  
Pain Behavior ◽  
Cancer Drug ◽  
Anti Cancer

Rhizoma Paridis saponins (RPS) as active parts ofP. polyphyllaSmith var.yunnanensishas been used as an anti-cancer drug in traditional Chinese medicine.

The Application of High Throughput Microarrays in the Screening Active Ingredients of Traditional Chinese Medicine

2014 ◽  
Vol 998-999 ◽  
pp. 346-349
Author(s):  
Qing Shan Liu ◽  
Wei Wei Zhang ◽  
Xu Li ◽  
Shu Juan Zhuang ◽  
Xiao Ying Yin
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
High Throughput ◽  
Detection Method ◽  
Active Ingredients ◽  
Chinese Medicines ◽  
Accurate Detection ◽  
And Control ◽  
Research Tools

The accurate detection of traditional Chinese medicine is significant for diagnosis, treatment and control for disease. There is an urgent need for the development of a rapid, simple, and accurate detection method. The high throughput microarray is recommended for use in all researches including those involving rare samples and expensive reagents. Due to the complexity of Chinese medicines interference and multi-target, multi-component, the advantages that sensitivity, reproducibility, and specificity of high-throughput microarrays make it become one of the effective research tools.

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