scholarly journals Effect of Cuttlebone on Healing of Indomethacin-Induced Acute Gastric Mucosal Lesions in Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Lifeng Qiu ◽  
Lingya Yao ◽  
Yanfei Fang ◽  
Lan Wang ◽  
Meng Xue ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Gastric Ulcers ◽  
Potential Candidate ◽  
Dependent Manner ◽  
Gastric Lesions ◽  
Gastric Mucosal Lesions ◽  
Antioxidant Parameters ◽  
Epidermal Growth ◽  
Mucosal Lesions ◽  
Underlying Mechanisms

The continuing use of nonsteroidal anti-inflammatory drugs (NSAIDs) usually increases the side effects such as peptic ulcer and acute gastric lesions in the gastrointestinal tract. Cuttlebone (CB), isolated from Sepiella maindroni de Rochebrune, was reported to have antioxidant activities, but its role in the treatment of indomethacin-induced gastric lesions has not yet been confirmed. In this research, we investigate the protective effect of cuttlebone on indomethacin-related ulcers in rats and possible mechanisms. Here, gastric ulcers were induced by oral administration of indomethacin, and then the rats were treated with omeprazole (4 mg/kg) or different doses (750, 1500, and 3000 mg/kg of body weight) of cuttlebone. We evaluated lesion index, inflammation score, and a series of oxidant/antioxidant parameters. The data demonstrated that cuttlebone could protect against gastric ulcers induced by indomethacin in a dose-dependent manner (positive correlation). Also, these effects were associated with attenuating the expression of malonaldehyde (MDA) and increasing the levels of some protective ingredients like epidermal growth factor (EGF), prostaglandin E2 (PGE2), and superoxide dismutase (SOD). Thus, considering its ability to protect indomethacin-induced acute gastric mucosal lesions and the underlying mechanisms, CB might be a potential candidate for treating gastric damage caused by NSAIDs.

scholarly journals Protective Effect of Pyrus ussuriensis Maxim. Extract against Ethanol-Induced Gastritis in Rats

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 439
Author(s):  
Naila Boby ◽  
Muhammad Aleem Abbas ◽  
Eon-Bee Lee ◽  
Zi-Eum Im ◽  
Walter H. Hsu ◽  
...  
Keyword(s):  
Therapeutic Option ◽  
Adenosine Monophosphate ◽  
Ethanol Ingestion ◽  
Dependent Manner ◽  
Protective Effects ◽  
Gastric Mucosal Lesions ◽  
Cellular Degeneration ◽  
Mucosal Lesions ◽  
Pyrus Ussuriensis Maxim

Pyrus ussuriensis Maxim (Korean pear) has been used for hundreds of years as a traditional herbal medicine for asthma, cough, and atopic dermatitis in Korea and China. Although it was originally shown to possess anti-inflammatory, antioxidant, and antiatopic properties, its gastroprotective effects have not been investigated. In the present study, we evaluated the protective effects of Pyrus ussuriensis Maxim extract (PUE) against ethanol-induced gastritis in rats. The bioactive compound profile of PUE was determined by gas chromatography mass spectroscopy (GC-MS) and high-performance liquid chromatography (HPLC). The gastroprotection of PUE at different doses (250 and 500 mg/kg body weight) prior to ethanol ingestion was evaluated using an in vivo gastritis rat model. Several endpoints were evaluated, including gastric mucosal lesions, cellular degeneration, intracellular damage, and immunohistochemical localization of leucocyte common antigen. The gastric mucosal injury and ulcer score were determined by evaluating the inflamed gastric mucosa and by histological examination. To identify the mechanisms of gastroprotection by PUE, antisecretory action and plasma prostaglandin E2 (PGE2), gastric mucosal cyclic adenosine monophosphate (cAMP), and histamine levels were measured. PUE exhibited significant antioxidant effects with IC50 values of 56.18 and 22.49 µg/mL for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′- azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) inhibition (%), respectively. In addition, GC/MS and HPLC analyses revealed several bioactive compounds of PUE. Pretreatment with PUE significantly (P < 0.05) decreased the ulcer index by preventing gastric mucosal lesions, erosion, and cellular degeneration. An immunohistochemical analysis revealed that PUE markedly attenuated leucocyte infiltration in a dose-dependent manner. The enhancement of PGE2 levels and attenuation of cAMP levels along with the inhibition of histamine release following PUE pretreatment was associated with the cytoprotective and healing effects of PUE. In contrast, the downregulation of the H+/K+ ATPase pathway as well as muscarinic receptor (M3R) and histamine receptor (H2R) inhibition was also involved in the gastroprotective effects of PUE; however, the expression of cholecystokinin-2 receptors (CCK2R) was unchanged. Finally, no signs of toxicity were observed following PUE treatment. Based on our results, we conclude that PUE represents an effective therapeutic option to reduce the risk of gastritis and warrants further study.

scholarly journals Gastroprotective Effects of Inulae Flos on HCl/Ethanol-Induced Gastric Ulcers in Rats

Molecules ◽  
2020 ◽  
Vol 25 (23) ◽  
pp. 5623
Author(s):  
Young-Sik Kim ◽  
Ji Hyeon Lee ◽  
Jungbin Song ◽  
Hocheol Kim
Keyword(s):  
Gastric Wall ◽  
Antioxidant Enzyme Activity ◽  
Mucus Secretion ◽  
Gastric Ulcers ◽  
Gastric Lesions ◽  
Gastric Mucus ◽  
Gastric Mucosal Lesions ◽  
Promising Agent ◽  
Inula Britannica ◽  
Inula Britannica L

Inulae Flos, the flower of Inula britannica L., is used as a dietary supplement, beverage, and medicine in East Asia. In this study, we evaluated the gastroprotective effects of Inulae Flos extract (IFE) against gastric mucosal lesions induced by hydrochloric acid (HCl)/ethanol in rats and explored its potential mechanisms by measuring antioxidant enzyme activity, mucus secretion, and prostaglandin E2 (PGE2) levels. Pretreatment with IFE at doses of 100 and 300 mg/kg significantly inhibited gastric lesions in HCl/ethanol-treated rats. IFE increased the activities of superoxide dismutase and catalase and the levels of glutathione and PGE2 in gastric tissues. The administration of IFE also significantly increased the gastric wall mucus contents in HCl/ethanol-induced gastric lesions. These findings suggest that IFE has gastroprotective effects against HCl/ethanol-induced gastric lesions and exerts these effects through increased antioxidant levels and gastric mucus secretion. Inulae Flos may be a promising agent for the prevention and treatment of gastritis and gastric ulcers.

Gastroprotective Effect of Fructus Evodiae Water Extract on Ethanol-Induced Gastric Lesions in Rats

2006 ◽  
Vol 34 (06) ◽  
pp. 1027-1035 ◽  
Author(s):  
Xiao Yu ◽  
Da-Zheng Wu ◽  
Jian-Ye Yuan ◽  
Rong-Rong Zhang ◽  
Zhi-Bi Hu
Keyword(s):  
Gastric Lesion ◽  
Ex Vivo ◽  
Water Extract ◽  
Mucosal Barrier ◽  
Dependent Manner ◽  
Gastric Lesions ◽  
Gastric Mucosal Lesions ◽  
Nitrate And Nitrite ◽  
Dose Dependent ◽  
Lesion Index

Fructus Evodiae is a widely used herbal medicine with anti-inflammatory and analgetic activities in China. The present study was designed to investigate the effect of Fructus Evodiae water extract (FE) on ethanol-induced gastric lesions in rats. Three hours before ethanol challenge, animals were intraperitoneally treated with FE (424.8 mg/kg, 141.6 mg/kg, and 47.6 mg/kg). Subsequently, we employed ex-vivo chamber technique to examine the effect of FE on gastric transmucosal potential difference (PD) changes. NOx (nitrate and nitrite) in gastric perfusate and gastric lesion index of whole glandular stomach were determined by intubation. The results showed that FE dose-dependently accelerated the recovery of PD reduction by ethanol, and increased NOx production in gastric perfusate. FE also inhibited gastric lesion formation in a dose-dependent manner. These results suggested that FE prevented ethanol-induced gastric mucosal lesions by strengthening the mucosal barrier integrity and increasing gastric mucosal nitric oxide (NO) synthesis.

Stress Gastric Ulcers and Cytoprotective Strategies: Perspectives and Trends

2020 ◽  
Vol 26 (25) ◽  
pp. 2982-2990 ◽  
Author(s):  
Arunabha Ray ◽  
Kavita Gulati ◽  
Peter Henke
Keyword(s):  
Treatment Strategies ◽  
Decisive Role ◽  
Experimental Models ◽  
Gastric Ulcers ◽  
Stress Profile ◽  
Gastric Lesions ◽  
Stress Ulceration ◽  
Gastric Mucosal Lesions ◽  
Relative Safety ◽  
Gastric Cytoprotection

Stress gastric ulceration is a clinical condition leading to morbidity/mortality and complex etiopathological factors are involved. Pharmacotherapy of such gastric mucosal lesions is not consistent and novel strategies are being explored. Targeting gastrointestinal factors have showed equivocal results and there is a possibility of involvement of extra-gastrointestinal factors. Stress is a highly interactive biological response in which the brain plays a key role. The involvement of brain substrates like the limbic system (amygdala, cortex, hippocampus) and behavioral traits has been investigated and research data has shown that the limbic brain-gut axis may be involved in the regulation of gastric mucosal integrity during stressful situations. The amygdaloid complex, its connections with other limbic structures and their neural networks act in tandem to contribute to both stress ulceration and gastroprotection. Complex neurotransmitter interactions in these areas involving biogenic amines and neuropeptides have been shown to modulate stress ulcerogenesis in experimental models. The immune system and brain-immune interactions also appear to play a decisive role in the genesis of such stress gastric lesions and the possibility of a brain-gut-immune axis has been proposed during stress gastric lesions. More recent studies have shown the involvement of oxidative stress and nitric oxide as well as their interactions during such stress gastric pathology, indicating the possible role of antioxidants and NO modulators as gastroprotective agents for stress ulceration. In view of the complex pathophysiology, multiple targets and lack of consistent therapeutic modalities, newer/alternative hypotheses are constantly emerging, which could be explored for effective treatment strategies aimed at gastric cytoprotection. Herbal agents with adaptogenic properties could be worth exploring in this regard as some of these phytopharmaceutical agents used in traditional medicine have been shown to exhibit gastric cytoprotection as part of their anti-stress profile. Further, their interactions with brain neurotransmitters and immune mechanisms and their relative safety could make them prospective leads for stress ulcer prophylaxis and treatment.

scholarly journals The Preventive Effect on Ethanol-Induced Gastric Lesions of the Medicinal PlantPlumeria rubra: Involvement of the Latex Proteins in the NO/cGMP/KATPSignaling Pathway

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Nylane Maria Nunes de Alencar ◽  
Rachel Sindeaux Paiva Pinheiro ◽  
Ingrid Samantha Tavares de Figueiredo ◽  
Patrícia Bastos Luz ◽  
Lyara Barbosa Nogueira Freitas ◽  
...  
Keyword(s):  
Folk Medicine ◽  
Primary Afferents ◽  
Gastrointestinal Disorders ◽  
Dependent Manner ◽  
Gastric Lesions ◽  
Preventive Effect ◽  
Underlying Mechanisms ◽  
Dose Dependent ◽  
Single Intravenous Administration ◽  
Plumeria Rubra

Plumeria rubra(Apocynaceae) is frequently used in folk medicine for the treatment of gastrointestinal disorders, hepatitis, and tracheitis, among other infirmities. The aim of this study was to investigate the gastroprotective potential of a protein fraction isolated from the latex ofPlumeria rubra(PrLP) against ethanol-induced gastric lesions and describe the underlying mechanisms. In a dose-dependent manner, the pretreatment with PrLP prevented ethanol-induced gastric lesions in mice after single intravenous administration. The gastroprotective mechanism of PrLP was associated with the involvement of prostaglandins and balance of oxidant/antioxidant factors. Secondarily, the NO/cGMP/KATPpathway and activation of capsaicin-sensitive primary afferents were also demonstrated as part of the mechanism. This study shows that proteins extracted from the latex ofP.rubraprevent gastric lesions induced in experimental animals. Also, the results support the use of the plant in folk medicine.

The guggulsterone derivative GG-52 inhibits NF-κB signaling in gastric epithelial cells and ameliorates ethanol-induced gastric mucosal lesions in mice

2013 ◽  
Vol 304 (2) ◽  
pp. G193-G202 ◽  
Author(s):  
Jung Mogg Kim ◽  
Su Hyun Kim ◽  
Su Hyuk Ko ◽  
Jireh Jung ◽  
Jaeyoung Chun ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mucosal Damage ◽  
Gastric Mucosal Damage ◽  
Mucosal Inflammation ◽  
Histological Evaluation ◽  
Dependent Manner ◽  
Gastric Epithelial Cells ◽  
Gastric Mucosal Lesions ◽  
Tnf Α ◽  
Mucosal Lesions

Gastric mucosal inflammation can develop after challenge with noxious stimuli such as alcohol. Specially, alcohol stimulates the release of inflammatory cytokines but does not increase gastric acid secretion, leading to gastric mucosal damage. The plant sterol guggulsterone and its novel derivative GG-52 have been reported to inhibit nuclear factor-κB (NF-κB) signaling in intestinal epithelial cells and experimental colitis. In the present study, we investigated the anti-inflammatory effects of GG-52 on gastric epithelial cells and on ethanol-induced gastric mucosal inflammation in mice. GG-52 inhibited the expression of interleukin-8 (IL-8) in gastric epithelial AGS and MKN-45 cell lines stimulated with tumor necrosis factor (TNF)-α in a dose-dependent manner. Pretreatment with GG-52 suppressed TNF-α-induced activation of IκB kinase (IKK) and NF-κB signaling in MKN-45 cells. In contrast, the inactive analog GG-46 did not produce significant changes in IL-8 expression or NF-κB activation. In a model of ethanol-induced murine gastritis, administration of GG-52 significantly reduced the severity of gastritis, as assessed by macroscopic and histological evaluation of gastric mucosal damage. In addition, the ethanol-induced upregulation of chemokine KC, a mouse homolog of IL-8, and phosphorylated p65 NF-κB signals were significantly inhibited in murine gastric mucosa pretreated with GG-52. These results indicate that GG-52 suppresses NF-κB activation in gastric epithelial cells and ameliorates ethanol-induced gastric mucosal lesions in mice, suggesting that GG-52 may be a potential gastroprotective agent.

Protective effect of human epidermal growth factor against the experimental gastric mucosal lesions in rats

Life Sciences ◽  
1990 ◽  
Vol 47 (12) ◽  
pp. 1031-1036 ◽  
Author(s):  
Harunobu Amagase ◽  
Teruo Murakami ◽  
Masafumi Misaki ◽  
Yutaka Higashi ◽  
Michiyasu Ushijima ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Protective Effect ◽  
Gastric Mucosal Lesions ◽  
Human Epidermal Growth Factor ◽  
Epidermal Growth ◽  
Mucosal Lesions

Isolation of a Wild Morchella spp. Strain and the Effects of its Extract on Ethanol-Induced Gastric Mucosal Lesions in Rats

2011 ◽  
Vol 66 (1-2) ◽  
pp. 55-62
Author(s):  
Wei Wei ◽  
Xia Luo ◽  
Linyong Zheng ◽  
Mengyao Yu ◽  
Nan Jiang ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Sequence Analysis ◽  
Phylogenetic Tree ◽  
Internal Transcribed Spacer ◽  
Protective Effects ◽  
Gastric Lesions ◽  
Sod Activity ◽  
Gastric Mucosal Lesions ◽  
Mucosal Lesions

A Morchella spp. strain was isolated from a wild morel mushroom, and the effects of its mycelia extract on the ethanol-induced gastric mucosal lesions of rats were investigated in vivo. Sequence analysis of internal transcribed spacer suggested that this Morchella spp. strain (strain No. M1) was clustered together with M. conica in the phylogenetic tree. The superoxide dismutase (SOD) activity increased significantly compared to the control. However, the malondialdehyde (MDA) level and myeloperoxidase (MPO) activity decreased significantly compared to the control. These results indicated that M1 is one member of M. conica and the protective effects of M1 extract against the ethanol-induced gastric lesions may be related to the increased SOD activity and decreased MDA level and MPO activity in rats.

Epidermal growth factor and transforming growth factor-??: role in protection and healing of gastric mucosal lesions

1995 ◽  
Vol 7 (10) ◽  
pp. 933-938 ◽  
Author(s):  
Peter Ch. Konturek ◽  
Stanislaw J. Konturek ◽  
Tomasz Brzozowski ◽  
Hans Ernst
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Transforming Growth Factor ◽  
Gastric Mucosal Lesions ◽  
Epidermal Growth ◽  
Mucosal Lesions

scholarly journals Gastro-Protective Effects of Albizia anthelmintica Leaf Extract on Indomethacin-Induced Gastric Ulcer in Wistar Rats: In Silico and In Vivo Studies

Antioxidants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 176
Author(s):  
Mohamed Nabil ◽  
Mohamed A. El Raey ◽  
Walied Abdo ◽  
Mohamed A. O. Abdelfattah ◽  
Assem M. El-Shazly ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
In Vivo Studies ◽  
Gastric Ulcers ◽  
Potential Candidate ◽  
Protective Effects ◽  
Ulcer Model ◽  
Anti Inflammatory

We have previously reported that the leaf extract of Albizia anthelmintica exhibited substantial antioxidant, anti-inflammatory, analgesic, and antipyretic properties in vivo. We also comprehensively characterized the active phytoconstituents and found several flavonoids and galloyl glucosides derivatives. In the current work, we explored the gastroprotective effects of the leaf extract in an indomethacin-induced ulcer model and the mechanisms involved. The rats being pretreated with the tested extract (100 and 200 mg kg−1) significantly prevented gastric lesions by 87.4% and 92.3%, respectively, and they had no structural derangements in the gastric mucosa. The extract significantly reduced the elevated levels of IKκB, NF-κB, TNF-α, IL-6, iNOS, and lipid peroxidation; increased the reduced level of glutathione peroxidase (GPx) activity; and reduced glutathione (GSH) in the indomethacin-induced ulcer model. The protective activities of the extract were similar in most aspects to those exerted by the known anti-ulcer drug famotidine. These activities might be attributed to the anti-inflammatory and antioxidant activities, and the reduction of iNOS levels. In conclusion, Albizia anthelmintica is a potential candidate for management of gastric ulcers with antioxidant properties.

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