scholarly journals Pemanfaatan Jeruk Purut (Citrus Hystrix) Sebagai Biolarvasida

2016 ◽  
Vol 1 (2) ◽  
Author(s):  
Titik Lestari
Keyword(s):  
Essential Oil ◽  
Literature Review ◽  
Aedes Aegypti ◽  
Research Methods ◽  
Chemical Compound ◽  
Mosquito Vector ◽  
The Third ◽  
Chemical Content ◽  
Lethal Time ◽  
Third Instar Larvae

Absctract: Citrus hystrix, Biolarvicides. Insectisides and pestisides were used as larvicides to control mosquito vector Aedes aegypti particularly. Some research suggested there had been a larvae resistance to pesticides. Biolarvicides with basic ingredients plant was another alternative that can be used. Lime (Citrus hystrix) was one of plants that often used as biolarvicides, both lits fruit and leaves. This paper aimed to know about chemical compound in lime and its potential as biolarvicidal. Research methods was literature review from several research journals. The results showed that the presence of coumpounds were essential oil, flavonoids, saponins, terpeesn, limonoids, citronellal, linalool, citronellol, acetate citronelil, kariofilin and geraniol. LC50 of lime leaves as biolarvicides againts the third instar larvae of Aedes aegypti was 279.882 ppm, biolarvicides oxicity values (LC90) was 4000 ppm with the lethal time (LT90) was 12.67 hours. Based on results of this study concluded that the presence of the chemical content of Citrus hystrix plant potential as biolarvicides.

scholarly journals Blue Tansy Essential Oil: Chemical Composition, Repellent Activity Against Aedes aegypti and Attractant Activity for Ceratitis capitata

2021 ◽  
Vol 16 (2) ◽  
pp. 1934578X2199019 ◽  
Author(s):  
Iris Stappen ◽  
Juergen Wanner ◽  
Nurhayat Tabanca ◽  
Ulrich R. Bernier ◽  
Paul E. Kendra
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
Ceratitis Capitata ◽  
Stationary Phases ◽  
Management Strategies ◽  
Fruit Fly ◽  
Effective Dosage ◽  
Mosquito Vector ◽  
Tea Tree ◽  
Potential Applications

Blue tansy essential oil (BTEO) ( Tanacetum annuum L.) was analyzed by GC-MS and GC-FID using two different capillary column stationary phases. Sabinene (14.0%), camphor (13.6%), myrcene (8.0%), β-pinene (7.7%), and chamazulene (6.9%) were the main components using an SE52 column (non-polar). On a polar CW20M phase column, sabinene (15.1%), camphor (14.4%), α-phellandrene (7.9%), β-pinene (7.7%), and myrcene (6.9%) were the most abundant compounds. To assess the oil for potential applications in integrated pest management strategies, behavioral bioassays were conducted to test for repellency against yellow fever mosquito Aedes aegypti, and for attractant activity for Mediterranean fruit fly Ceratitis capitata. Results showed that BTEO was not effective in repelling Ae. aegypti (minimum effective dosage [MED]: 0.625 ± 0.109 mg/cm2 compared with the standard insect repellent DEET (N,N-diethyl-3-methylbenzamide). In assays with male C. capitata, BTEO displayed mild attraction compared with two positive controls (essential oils from tea tree Melaleuca alternifolia and African ginger bush Tetradenia riparia). Additional studies are needed to identify the specific attractant chemicals in BTEO and to determine if they confer a synergistic effect when combined with other known attractants for C. capitata. To the best of our knowledge, this study represents the first investigation of BTEO for repellency against the mosquito vector Ae. aegypti and for attractancy to C. capitata, a major agricultural pest worldwide.

scholarly journals Transcriptomic analysis and molecular docking reveal genes involved in the response of Aedes aegypti larvae to an essential oil extracted from Eucalyptus

2021 ◽  
Vol 15 (7) ◽  
pp. e0009587
Author(s):  
Ivana Sierra ◽  
Jose Manuel Latorre-Estivalis ◽  
Lucila Traverso ◽  
Paula V. Gonzalez ◽  
Ariel Aptekmann ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Essential Oil ◽  
Aedes Aegypti ◽  
Vector Control ◽  
Gene Families ◽  
Control Group ◽  
Mosquito Vector ◽  
Rna Seq ◽  
Chemosensory Proteins ◽  
The Impact

Background Aedes aegypti (L.) is an urban mosquito, vector of several arboviruses that cause severe diseases in hundreds of million people each year. The resistance to synthetic insecticides developed by Ae. aegypti populations worldwide has contributed to failures in vector control campaigns, increasing the impact of arbovirus diseases. In this context, plant-derived essential oils with larvicidal activity could be an attractive alternative for vector control. However, the mode of action and the detoxificant response of mosquitoes to plant derived compounds have not been established, impairing the optimization of their use. Methods and findings Here we compare gene expression in Ae. aegypti larvae after 14 hrs of exposure to Eucalyptus camaldulensis essential oil with a control group exposed to vehicle (acetone) for the same lapse, by using RNA-Seq. We found differentially expressed genes encoding for cuticle proteins, fatty-acid synthesis, membrane transporters and detoxificant related gene families (i.e. heat shock proteins, cytochromes P450, glutathione transferases, UDP-glycosyltransferases and ABC transporters). Finally, our RNA-Seq and molecular docking results provide evidence pointing to a central involvement of chemosensory proteins in the detoxificant response in mosquitoes. Conclusions and significance Our work contributes to the understanding of the physiological response of Ae. aegypti larvae to an intoxication with a natural toxic distilled from Eucalyptus leafs. The results suggest an involvement of most of the gene families associated to detoxification of xenobiotics in insects. Noteworthy, this work provides important information regarding the implication of chemosensory proteins in the detoxification of a natural larvicide. Understanding the mode of detoxification of Eucalyptus distilled compounds could contribute to their implementation as a tool in mosquito control.

Toxic effect of essential oil and its compounds isolated from Sphaeranthus amaranthoides Burm. f. against dengue mosquito vector Aedes aegypti Linn.

2019 ◽  
Vol 160 ◽  
pp. 163-170 ◽  
Author(s):  
Annamalai Thanigaivel ◽  
Kanagaraj Muthu-Pandian Chanthini ◽  
Sengodan Karthi ◽  
Prabhakaran Vasantha-Srinivasan ◽  
Athirstam Ponsankar ◽  
...  
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
Toxic Effect ◽  
Mosquito Vector

scholarly journals Chemical Composition and Larvicidal Activity of Eugenia triquetra Essential Oil from Venezuelan Andes

2010 ◽  
Vol 5 (6) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Flor D. Mora ◽  
Jorge L. Avila ◽  
Luis B. Rojas ◽  
Rosslyn Ramírez ◽  
Alfredo Usubillaga ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Chemical Constituents ◽  
Ms Analysis ◽  
Third Instar Larvae

The chemical constituents of the essential oil obtained by hydrodistillation of the leaves of Eugenia triquetra O. Berg, collected in Táchira State, Venezuela, were identified by GC-MS analysis. Twenty-six components, which made up 88.5% of the oil, were identified. The major constituents were linalool (17.5%), limonene (16.9%), α-pinene (11.6%), β-pinene (8.7%), and p-cymene (3.7%). The essential oil was tested against third-instar larvae of Aedes aegypti, showing a LC50 value of 64.8 ± 5.6 ppm.

scholarly journals Priprioca (Cyperus articulatus var. nodosus, Cyperaceae) Hydrolate as Larvicidal against Aedes aegypti

2020 ◽  
pp. 91-98
Author(s):  
Inês Ribeiro Machado ◽  
Keila Rêgo Mendes ◽  
Adriano Rodrigues De Paula ◽  
Michelly Rios Arévalo ◽  
Amanda Sousa Silva ◽  
...  
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
Plant Extracts ◽  
Chemical Characterization ◽  
Bioactive Substances ◽  
Commercial Products ◽  
Larvicidal Effect ◽  
The Third ◽  
Hydrolysis Of

Dengue cases have grown significantly in Brazil in recent years. Studies with plant extracts show the insecticidal potential of bioactive substances that become candidates for future commercial products. This work evaluated the larvicidal effect of essential oil, without hydrolysis of Cyperus articulatus var. nodosus against the third and fourth wide stage of Aedes aegypti, which reduces larvae survival by as much as the 3th and 4th instar of Ae. aegypti. A chemical characterization of the hydrogen skeletons was performed by the GC-MS, revealing verbenone (%), trans-sabinol (%) and mirtenol (%) as major compounds. 


scholarly journals New Purinyl-Steroid and Other Constituents from the Marine Fungus Penicillium brefeldianum ABC190807: Larvicidal Activities against Aedes aegypti

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Zong-Min Hou ◽  
Sen-Quan Yu ◽  
Min Tao ◽  
Cai-Bei Xia ◽  
Yu-Lian Xia ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
2D Nmr ◽  
Marine Fungus ◽  
Mangrove Sediment ◽  
Health Issues ◽  
Etoac Extract ◽  
The Third ◽  
Larvicidal Activities ◽  
Third Instar Larvae

Mosquitoes historically threatened human health; the major mosquito-related global health issues include malaria, dengue fever, yellow fever, and Zika, as well as several other vector-borne outbreaks. Here, the EtOAc extract of the mangrove sediment fungus Penicillium brefeldianum ABC190807 exerted larvicidal activities against the third instar larvae of Aedes aegypti with an LC50 of 0.089 mg/mL. One new purinyl-steroid (ergosta-4,6,8(14),22-tetraen-3-(6-amino-9H-purin-9-yl) (1)), along with six (2–7) known compounds, were isolated from the EtOAc extract of Penicillium brefeldianum ABC190807. Structures of the compounds were elucidated via 1D/2D NMR and HR-ESI-MS data. Respective spectral data were compared with those of known compounds. Among all compounds whose larvicidal activity against the third instar larvae of Aedes aegypti was evaluated, compounds 2 and 7 showed larvicidal activity with respective LC50 values of 0.452 and 0.337 mg/mL.

scholarly journals Susceptibility of Aedes aegypti larvae to temephos and Bacillus thuringiensis var israelensis in integrated control

1991 ◽  
Vol 25 (3) ◽  
pp. 184-187 ◽  
Author(s):  
Carlos Fernando S. de Andrande ◽  
Maurício Modolo
Keyword(s):  
Bacillus Thuringiensis ◽  
Aedes Aegypti ◽  
Integrated Control ◽  
Integrated Treatment ◽  
Fourth Instar ◽  
Two Agents ◽  
Lethal Time ◽  
Third Instar Larvae

The susceptibility of field collected Aedes aegypti larvae was evaluated in terms of median lethal time (LT50) and final mortality, when treated with temephos, Bacillus thuringiensis var israelensis as well as mixtures of these two agents. Third instar larvae were shown to be more susceptible than early and late fourth instar ones to the entomopathogen. Survival of some individuals when exposed to temephos suggest possible resistance. Temporal synergism in early fourth instar larvae was detected when they were exposed to mixtures of Bti-temephos. The possibility of this integrated treatment is commented on.

scholarly journals Integrated Control of Aedes Aegypti With Parasitic Nematodes, Biological Control Agents, Chemical Insecticides and Plant Essential Oil

2021 ◽  
Author(s):  
Jinfeng Xiong ◽  
Hui Zhang ◽  
Caixia Li ◽  
Rui Ma ◽  
Hui Ai ◽  
...  
Keyword(s):  
Biological Control ◽  
Essential Oil ◽  
Aedes Aegypti ◽  
High Efficiency ◽  
Pcr Analysis ◽  
Control Group ◽  
Mosquito Vector ◽  
Biological Control Agents ◽  
Competitive Binding Assay ◽  
Density Control

Abstract Aedes aegypti can transmit dengue fever, yellow fever, Chikungunya fever, Zika virus disease and vector density control is the most effective way to prevent these infectious diseases. However, the extensive use of chemical pesticides has caused a series of problems, such as environmental pollution, killing non-target organisms and so on. In this study, a parasitic nematode, Romanomermis wuchangensis was used in the larviciding evaluation of Ae. aegypti, while the activity of four chemical insecticides and biological control agents were tested. Besides, Mentha haplocalyx essential oil was isolated and its olfactory physiological function with OBP1 protein of Ae. aegypti antenna was measured by the prokaryotic expression and fluorescence competitive binding assay. Compared with the control group, R. wuchangensis indicated high efficiency and environmental friendliness in the control of Ae. aegypti. After the second instar larvae were parasitized, the mortality of two treatment groups exceeded 75%. Compared to control group, the quantitative real-time PCR analysis results demonstrated that SOD, POD and CAT genes had obvious high expression levels in the nematodes parasitic groups. The antioxidant enzyme test results also exhibited obvious difference of SOD, CAT and POD during the nematode parasitic period. Besides, Bacillus thuringiensis (Bti) and chemical insecticide experimental results also showed great insecticidal efficacy against mosquito larvae. Five chemical components including Menthol, Pinene, Limonene, Isopulegol and Pulegone were identified from M. haplocalyx and exhibited great binding ability with AaegOBP1 protein. Present results illustrated that the integrated application of these various mosquito vector control methods in the future has broad prospects.

scholarly journals Larvicidal Activity against Aedes Aegypti of Essential Oils from Northeast Brazil

2012 ◽  
Vol 7 (10) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Patrícia L. Lavor ◽  
Gilvandete M. P. Santiago ◽  
Roberto W. da Silva Gois ◽  
Leôncio M. de Sousa ◽  
Gabrieli da P. Bezerra ◽  
...  
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
Essential Oils ◽  
Larvicidal Activity ◽  
Northeast Brazil ◽  
Artemisia Vulgaris ◽  
Cymbopogon Flexuosus ◽  
Larvicidal Activities ◽  
Third Instar Larvae

The larvicidal activities of essential oils from the leaves of Artemisia vulgaris L., Cymbopogon flexuosus(Nees ex Steud.) Wats. and Piper tuberculatum Jacq. were evaluated using third-instar larvae of Aedes aegypti. The essential oils were obtained by hydrodistillation and analyzed by GC and GC-MS. The essential oil of P. tuberculqatum had the lowest LC50 value (106.3 ± 2.2 μg/mL), followed by that of A. vulgaris(114.1 ± 1.7 μg/mL) and C. flexuosus(121.6 ± 0.8 μg/mL). The results show that these essential oils may be potent sources of natural larvicides.

scholarly journals Larvicidal Activity of Ethyl Acetate Extract of Derris elliptica Root against the Third-instar Larvae of Cypermethrin-Resistant Aedes aegypti Offspring

2021 ◽  
Author(s):  
Sayono Sayono ◽  
Risyandi Anwar ◽  
Didik Sumanto
Keyword(s):  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Larvicidal Activity ◽  
Ethyl Acetate Extract ◽  
Larval Mortality ◽  
Laboratory Strain ◽  
The Third ◽  
Resistant Strains ◽  
Four Levels ◽  
Third Instar Larvae

Background: Derris elliptica extracts have a high larvicidal potential against the laboratory strain of Aedes aegypti larvae, but the effect on offspring larvae of pyrethroid-resistant strains of the species is lack understood. This study aimed to determine the larvicidal activity of the ethyl acetate extract of tuba root against the third-instar larvae of the Cypermethrin-resistant Ae. aegypti offspring. Methods: The experimental study occupied four levels of ethyl acetate extract of D. elliptica namely 10, 25, 50, and 100 ppm, and each level was four times replicated. As many as twenty of healthy third-instar larvae, offspring of Cypermethrin-resistant Ae. aegypti were subjected to each experiment group. Larval mortality rate and lethal concentration 50% subject (LC50) were calculated after 24 and 48 hours of exposure time. Results: Mortality of larvae increased directly proportional to the increase of extract concentration. Larval mortality rates after 24 and 48 hours of exposure were 40–67.5% and 62.5–97.5%, and LC50 were 34.945 and 6.461ppm, respectively. Conclusion: The ethyl acetate extract of D. elliptica has the high effectiveness larvicidal potential against the thirdinstar larvae, offspring of the Cypermethrin-resistant Ae. aegypti. Isolation of the specific compound is necessarily done to obtain the active ingredient for larvicide formulation.

Sign in / Sign up

Export Citation Format

Share Document