scholarly journals Effect of Botanical Extracts on Late Blight (Phytopthora infestans) and Productivity of Tomato (Solanum esculentum)

2021 ◽  
Vol 2021 ◽  
pp. 1-19
Author(s):  
T. Choga ◽  
E. Ngadze ◽  
J. T. Rugare ◽  
S. Mabasa ◽  
W. Makaza ◽  
...  
Keyword(s):  
Late Blight ◽  
Ethyl Acetate ◽  
Radial Growth ◽  
Disease Incidence ◽  
Plant Production ◽  
Water Extracts ◽  
Percent Inhibition ◽  
Extract Concentration ◽  
Botanical Extracts ◽  
Botanical Extract

Late blight is caused by Phytopthora infestans (Mont.) de Bary which establishes quickly in Solanum esculentum L. (tomato); as a result, it makes the pathogen one of the most devastating plant diseases across the world. The control of late blight is difficult because P. infestans has advanced and complex enzymes and effecter molecules coded by avirulence genes. As such, a study was carried out at the University of Zimbabwe, Department of Plant Production Sciences and Technologies, between August 2018 and May 2019 to evaluate the efficacy of Moringa oleifera Lour. (moringa), Eucalyptus nigra R.T. Baker (gumtree), and Lantana camara L. (sensu lato) (lantana) extracts as biofungicides on late blight development on S. esculentum. The effect of acetone, ethyl acetate, and water extracts of M. oleifera, E. nigra, and L. camara on percent inhibition and radial growth were evaluated in the laboratory. In addition, the efficacy of different extract concentrations of M. oleifera, E. nigra, and L. camara L. on defence enzymes, disease incidence, disease severity, and yield parameters of S. esculentum were assessed in the in vivo experiment. Increasing concentration of the botanical extracts significantly ( p ≤ 0.05 ) reduced radial growth and increased percent inhibition of P. infestans. In addition, increasing botanical extract concentration significantly ( p ≤ 0.05 ) increased peroxidase (PОD), phenylalanine ammonia lyse (PAL), and polyphenol oxidase (PPO) activity. Disease incidence and severity were significantly ( p ≤ 0.05 ) reduced as the concentration of the botanical extracts increased. Similarly, marketable and total yield significantly ( p ≤ 0.05 ) increased with increase in the botanical extract concentration. Gas chromatography mass spectrometry (GCMS) revealed the presence of furfural, 5-methyl-4- (trifluoromethyl) pyrido, dodecanamide, cyclopentasiloxane, and decamethyl. From the research, it can be concluded that M. oleifera ethyl acetate, E. nigra ethyl acetate, and L. camara water extracts contain antifungal compounds and can be used as biofungicides in late blight management.

scholarly journals In-Vitro Efficacy of Different Botanical Extract against Leaf Spot of Marry Gold caused by Alternaria alternat

2021 ◽  
Vol 1 (1) ◽  
pp. 05-08
Author(s):  
Keyword(s):  
Alternaria Alternata ◽  
Leaf Spot ◽  
Disease Incidence ◽  
Colony Growth ◽  
Garlic Extract ◽  
Ginger Extract ◽  
Neem Extract ◽  
Botanical Extracts ◽  
Botanical Extract

This study was carried out to study the in-vitroefficacy of different botanical extracts against leaf spot of marigold, caused by Alternaria alternata. The results of present study showed that the disease incidence from variouslocations of district HyderabadSindhwas observed Ghangra Mori35%, TandoHyder 30%, Rahooki25%, Moosa Khatyan20%, and Hosri15%.Upper limitdisease occurrencewas observedfrom Ghangra Mori and lower limit sickness was recordedfrom Hosri. The botanicalplantextract ‘Neem’ observed 48, 40and 34mmlinear colony growth of Alternaria alternataat 2%, 4% and 6% concentrations, respectively, Dhaturaextract observed 75.5, 63 and 57.5mmcolony growth of Alternaria alternataat 2%, 4% and 6% concentrations, respectively. Garlic extract observed 49%, 43% and 36% linear colony growth of Alternaria alternataat 2%, 4% and 6% concentrations, respectively. Ginger extract observed 50, 43.5and 36.5mmcolony growth of Alternaria alternataat 2%, 4% and 6% concentrations, respectively. Minimum linear colony growth of Alternaria alternatawas observed at 6% concentration for Neem extract followed by Garlic extract, Ginger extract and Datura extract, respectivelythe plant infusion, Neeminfusion show good effectoppositelinear colony growth of Alternaria alternataon6% solution

scholarly journals Potential Anticancer Activity of Crude Ethanol, Ethyl Acetate, and Water Extracts of Ephedra foeminea on Human Osteosarcoma U2OS Cell Viability and Migration

2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
Eric Zadok Mpingirika ◽  
Ahmed El Hosseiny ◽  
Sheri Magdy Saleeb Bakheit ◽  
Rami Arafeh ◽  
Asma Amleh
Keyword(s):  
Steady State ◽  
Cell Viability ◽  
Ethyl Acetate ◽  
Anticancer Activity ◽  
Ethanol Extract ◽  
U2os Cell ◽  
Mrna Levels ◽  
Water Extracts ◽  
Human Osteosarcoma ◽  
And Migration

Medicinal plants are potential sources for a wide range of complex compounds with probable anticancer activity. Ephedra foeminea Forssk. (E. foeminea), a medicinal plant found in the Eastern Mediterranean, has recently been gaining popularity as a cancer remedy; there is, however, a paucity of empirical evidence supporting this claim. In this study, the effect of E. foeminea ethyl acetate, ethanol, and water crude extracts on viability, migratory ability, and the steady-state mRNA levels of genes involved in these processes was, respectively, examined using MTT assay, wound healing assay, and reverse transcriptase PCR (RT-PCR). The study concludes that all extracts significantly reduce human osteosarcoma U2OS percentage viability in a dose- and time-dependent manner, with varying potencies. The least half-maximal inhibitory concentration (IC50) was observed in the water extract after 48 h incubation (30.761±1.4 μg/mL) followed by the ethyl acetate extract after 72 h incubation (80.35±1.233 μg/mL) and finally the ethanol extract after 48 h incubation (97.499±1.188 μg/mL). Ethanol extract significantly reduced U2OS percentage wound closure. On the other hand, both ethanol and water extracts considerably reduced the steady-state mRNA expression of beta-catenin, promoting both cell proliferation and migration in osteosarcoma by regulating target genes. Additionally, E. foeminea showed no hemolytic activity. These effects suggest that E. foeminea decreases U2OS cell viability and migratory ability by modulating the expression of critical genes involved in regulating these processes and is likely cytocompatible with human erythrocytes.

scholarly journals COMPARISON OF INHIBITORY ACTIVITY AGAINST THE α-GLUCOSIDASE ENZYMES IN THE EXTRACTS AND FRACTIONS FROM LEAVES OF THE GARCINIA KYDIA ROXBURGH

2017 ◽  
Vol 10 (7) ◽  
pp. 401
Author(s):  
Septiani Martha ◽  
Berna Elya ◽  
Muhammad Hanafi
Keyword(s):  
Ethyl Acetate ◽  
Inhibitory Activity ◽  
Enzymatic Reaction ◽  
Active Fraction ◽  
Pharmacological Activities ◽  
Percent Inhibition ◽  
Ic50 Values ◽  
Microplate Reader ◽  
Ethyl Acetate Extracts

Objective : Garcinia kydia Roxb. is aspecies of the genus Garcinia, is based chemotaxonomic has various bioactive compounds that have been isolated by a variety of pharmacological activities, one of the activities that are being developed that inhibition of         α-glucosidase. However, α-glucosidase inhibitory activity in the extracts and fraction from leaves of the Garcinia kydia Roxb. has not been reported. In this study, seeks to evaluated of α-glucosidase inhibitory activity against extracts and fractions of potentially.Methods : The α-glucosidase inhibitory activity test, conducted by in-vitro using the enzymatic reaction is measured of quantity with a microplate reader and identify the compound from the active fraction with normal-phase thin layer chromatography.Results : The ethyl acetate and methanol extract have the potential to inhibit the α-glucosidase with the percent inhibition at a concentration of 500μg/mL of 83 and 59%, respectively. The active fraction of the ethyl acetate extracts (FEA8) with percent inhibition at concentrations of 100 mg/mL and IC50 values of 80% and 2,79μg/mL, respectively and active fraction of the methanol extracts (FMT3) with percent inhibition at concentrations of 100 mg/mL and IC50 values of 71% and 8,43 μg/mL, respectively.Conclusion: Garcinia kydia Roxb. evident has the potential to inhibit the α-glucosidase. Flavonoid and phenolic compounds that suspected of acts as α-glucosidase inhibitory activity. Thus, the research will continue the process of isolating the active compound so that it can be developed as natural therapeutic agents in the control of glucose.

Efficacy of fungicides in controlling late blight of potato

2015 ◽  
Vol 26 (2) ◽  
pp. 103-108
Author(s):  
MM Anwar ◽  
A Parveen ◽  
MM Hossain ◽  
NU Mahamud ◽  
RK Roy
Keyword(s):  
Late Blight ◽  
Tuber Yield ◽  
Cultural Practices ◽  
Disease Incidence ◽  
Integrated Approach ◽  
Control Treatment ◽  
Commercial Potato ◽  
Late Blight Disease ◽  
Blight Disease ◽  
Late Blight Of Potato

Potato cultivars grown in Bangladesh have low levels of general resistance to late blight. As such, most commercial potato farmers rely on fungicide applications for control of Phytophthora infestans, the causal agent of late blight. Management of late blight of potato requires an integrated approach that includes rotation with non-hosts, resistant cultivars, cultural practices, and fungicides. The study on efficacy of some new fungicides against late blight disease of potato was conducted at ARS, Alamnagar Rangpur during rabi season 2010-2011 to select suitable fungicides against late blight of potato. Thirteen different fungicides were tested and all the tested fungicides showed significantly better performance over control. Considering percentage disease incidence T4,T6 and T12 showed better performance than all other treatment. In case of T4,T6 and T12 treatment disease reduction was more than 80 % over control. Significantly the highest tuber yield 25.5 t ha-1was obtained from T3  which was statistically similar to the yield of T2,T5 , T6, T9, T10, T11and T12  treatment whereas the lowest tuber  yield 14.5 t ha-1 was obtained from control treatment. Field experiment was conducted from 2010 to 2011 to investigate the comparative efficacy of the fungicides. In the field, applications of fungicide that preceded the largest incremental increase in disease incidence provided the best control of disease or increased yield.Progressive Agriculture 26 (2): 103-108, 2015

Department of Plant Pathology, Swami Keshwanand Rajasthan Agricultural University, Bikaner-334 001, Rajasthan, India.

2020 ◽  
Author(s):  
Mohit . Kumar ◽  
Data Ram Kumhar ◽  
Ashok Kumar Meena ◽  
Kiran . Choudhary
Keyword(s):  
Plant Pathology ◽  
Grain Yield ◽  
Root Rot ◽  
Seed Treatment ◽  
Disease Incidence ◽  
Soil Application ◽  
Percent Inhibition

Bio-control agents and bio-fertilizer minimized the root rot incidence, increased percent inhibition, increased grain yield and nodulation in mungbean significantly as compared to control. The minimum 13.50% disease incidence, maximum 79.23% inhibition of dry root rot, maximum grain yield of 14.8q/ha and maximum 24 nodules/plant were observed in the treatment T13. Where T. harzianum in combination with Rhizobium was used as seed treatment and soil application followed by the treatment(T14) where P. fluorescens was used in combination with Rhizobium (18.50% disease incidence, 71.54% disease inhibition,13.4q/ha grain yield and 21 nodules/plant. used as seed treatment and soil application.

A comparative in vitro and in silico study of the biological potential and chemical fingerprints of Dorcycinum pentapyllum subsp. haussknechtii using three extraction procedures

2017 ◽  
Vol 41 (22) ◽  
pp. 13952-13960 ◽  
Author(s):  
Sengul Uysal ◽  
Abdurrahman Aktumsek ◽  
Carene M. N. Picot ◽  
Alime Sahan ◽  
Adriano Mollica ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
In Silico ◽  
Water Extracts ◽  
Extraction Procedures ◽  
Chemical Fingerprints ◽  
Biological Potential ◽  
In Silico Study

Ethyl acetate, methanol, and water extracts prepared by maceration, Soxhlet, and ultrasonication were profiled and studied using in vitro and in silico methodologies.

scholarly journals Epidemiology and Management of Petunia and Tomato Late Blight in the Greenhouse

Plant Disease ◽  
2005 ◽  
Vol 89 (9) ◽  
pp. 1000-1008 ◽  
Author(s):  
M. C. Becktell ◽  
M. L. Daughtrey ◽  
W. E. Fry
Keyword(s):  
Bacillus Subtilis ◽  
Lycopersicon Esculentum ◽  
Late Blight ◽  
Plant Defense ◽  
Trichoderma Harzianum ◽  
Petunia Hybrida ◽  
Disease Incidence ◽  
The Other ◽  
Factors Affecting ◽  
Overhead Irrigation

Factors affecting the management of petunia and tomato late blight, caused by Phytophthora infestans, under greenhouse conditions were investigated. Late blight-infected petunias (Petunia × hybrida) and tomatoes (Lycopersicon esculentum) each produced sporangia that were dispersed throughout the greenhouse via air currents. Infected petunias produced and released fewer sporangia than infected tomatoes, but infected petunias released sporangia two times longer. Surface-directed irrigation reduced disease incidence compared with overhead irrigation that wetted the foliage. The fungicides dimethomorph-mancozeb, fosetyl-Al, azoxystrobin, and dipo-tassium phosphonate/phosphate suppressed late blight development, as did the plant defense activator acibenzolar-S-methyl. All products were applied twice at 7-day intervals. The other plant defense activator (harpin protein) and the bioantagonists (Trichoderma harzianum, Glio-cladium virens, and Bacillus subtilis) were ineffective at the rates tested.

Light-induced alteration of leaf sterol content and late blight disease development in tomato

1982 ◽  
Vol 60 (12) ◽  
pp. 2724-2728 ◽  
Author(s):  
B. Bradford ◽  
L. D. Moore ◽  
D. M. Orcutt
Keyword(s):  
Light Intensity ◽  
Late Blight ◽  
Disease Incidence ◽  
Total Sterol ◽  
Disease Development ◽  
Low Light ◽  
Light Intensities ◽  
Intensity Changes ◽  
Late Blight Disease ◽  
Blight Disease

‘Nova’ and 'Beefsteak,' cultivars of tomato (Lycopersicon esculentum Mill.), were grown in chambers under light intensities of 240 or 120 μE∙m−2∙s−1. Thirty-five days after seeding, half of the tomato plants were harvested for sterol analysis and the others were inoculated with a tomato race O isolate of Phytophthora infestons (Mont.) de By. Late blight symptoms were assessed 10 days after inoculation from the number of leaves showing disease (disease incidence) and the amount of blighted area of each leaf (percent colonization). Disease incidence and percent colonization were not influenced by light intensity with 'Nova' plants, but 'Beefsteak' plants grown under low light were significantly [Formula: see text] more diseased than those exposed to the higher light intensity. Changes in free sterol, steryl ester, and total sterol concentrations between plants grown under different light intensities were similar for both cultivars. A significant decrease in the steryl glycoside concentration of low light grown 'Beefsteak' plants correlated with increased disease incidence. The possible role of steryl glycosides and their derivatives in late blight disease development is discussed.

scholarly journals Aerial Photography Used for Spatial Pattern Analysis of Late Blight Infection in Irrigated Potato Circles

2003 ◽  
Vol 93 (7) ◽  
pp. 805-812 ◽  
Author(s):  
Dennis A. Johnson ◽  
J. Richard Alldredge ◽  
Philip B. Hamm ◽  
Bruce E. Frazier
Keyword(s):  
Late Blight ◽  
Aerial Photography ◽  
Large Scale ◽  
Temporal Dynamics ◽  
Disease Incidence ◽  
Aerial Photographs ◽  
Spatial Pattern Analysis ◽  
Initial Inoculum ◽  
Disease Patterns ◽  
Commercial Potato

Spatial and temporal dynamics of late blight were investigated from color, infrared aerial photographs of five commercial potato fields in the Columbia Basin during epidemics in 1993, 1995, and 1998. Aerial photographs were taken one to four times at 6- to 21-day intervals. Photographs were scanned and pixels, representing approximately 1 m2 in the field, were used in the analysis. Late blight-infected plants were aggregated as indicated by runs analysis. Significant z-tests were computed for four directions during each sampling date in each of the five fields. Absolute z-values for runs analysis increased, indicating increasing aggregation in the four directions, as disease incidence increased in the early and midphases of the epidemics in each field. Variograms indicated the existence of autocorrelation among infected plants in four directions; the range of influence increased as disease incidence increased except at the highest levels of disease. Late blight was observed to spread in fields as foci. Late blight foci enlarged in size, produced distinct daughter foci, and coalesced. A field where initial inoculum likely originated from infected seed tubers exhibited less initial aggregation than the other fields, perhaps due to a different source of primary inoculum. Aerial photography coupled with spatial analyses of late blight-infected plants was an effective technique to quantitatively assess disease patterns in relatively large fields and was useful in quantifying an intensification of aggregation during the epidemic process on a large scale.

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