Fine-Mapping of Xq25.1–27.2 Shows Association of Early Childhood Caries with Genetic Variants Depending on Dietary Habits, Protecting Children Who Drink Milk before Going to Bed

2018 ◽  
Vol 53 (3) ◽  
pp. 333-338 ◽  
Author(s):  
Zerrin Abbasoğlu ◽  
Diego G. Bussaneli ◽  
İlknur Tanboğa ◽  
Indiara W. Henn ◽  
Adriana Modesto ◽  
...  
Keyword(s):  
Early Childhood ◽  
X Chromosome ◽  
Early Childhood Caries ◽  
Dietary Habits ◽  
Chromosome Region ◽  
Caries Experience ◽  
Design Data ◽  
Cross Sectional ◽  
Cross Sectional Design ◽  
Childhood Caries

Early childhood caries (ECC) is a chronic, infectious disease that affects the primary dentition of young children. It is the result of unequal contributions of risk factors and protective factors that influence the disease. The aim of this study was to assess if the X chromosome region previously linked to caries was associated with ECC. Two hundred and fifty-nine unrelated children with no chronic illnesses from 2 to 5 years of age who had no systemic fluoride consumption were evaluated using a cross-sectional design. Data on oral habits were obtained through a questionnaire, and caries experience data were collected by clinical examination. Twenty-three markers in ten genes were studied. Genotyping of the selected polymorphisms was carried out by real-time polymerase chain reaction. Regression analyses were performed comparing individuals with and without caries experience. Of 259 subjects, 123 were caries free. The markers in Xq25.1–27.2 were associated with ECC when children were using milk bottle for longer times (p = 0.01) and had more snacks over the course of a day (p = 0.05). Conversely, the markers in the X chromosome studied here were protective for ECC (p = 0.008) in children consuming milk before going to sleep. The genes located in the X chromosome possibly contribute to ECC and have an impact on ECC depending on the dietary habits.

scholarly journals Early Childhood Caries Is Associated with Genetic Variants in Enamel Formation and Immune Response Genes

2014 ◽  
Vol 49 (1) ◽  
pp. 70-77 ◽  
Author(s):  
Zerrin Abbasoğlu ◽  
İlknur Tanboğa ◽  
Erika Calvano Küchler ◽  
Kathleen Deeley ◽  
Megan Weber ◽  
...  
Keyword(s):  
Risk Factors ◽  
Early Childhood ◽  
Environmental Factors ◽  
Early Childhood Caries ◽  
Caries Experience ◽  
Design Data ◽  
Cross Sectional ◽  
Cross Sectional Design ◽  
Genetic And Environmental Factors ◽  
Childhood Caries

Early childhood caries (ECC) is a chronic, infectious disease that affects the primary dentition of young children. It is the result of an imbalance of risk factors and protective factors that influence the disease. The aim of this study was to assess genetic and environmental factors that may contribute to ECC. Two hundred and fifty-nine unrelated children were evaluated using a cross-sectional design. Data on oral habits were obtained through a questionnaire, and caries experience data were collected by clinical examination. Twenty-three markers in 10 genes were studied. Genotyping of the selected polymorphisms was carried out by real-time PCR. Regression analyses were performed comparing individuals with and without caries experience. Of 259 subjects, 123 were caries free. The genotype TT in ALOX15 (rs7217186) was a risk factor for ECC, whereas the genotypes GG in ENAM (rs1264848), AG and GG in KLK4 (rs198968), CT in LTF (rs4547741), and GG in TUFT1 (rs3790506) were protective for EEC. In conclusion, environmental factors and gene interactions can act as protective or risk factors for ECC. These factors together contribute to the presence and severity of the disease.

scholarly journals Association of Feeding Methods and Streptococcus mutans Count with Early Childhood Caries: A Cross-sectional Study

2017 ◽  
Vol 10 (2) ◽  
pp. 119-125 ◽  
Author(s):  
Manjunath P Puranik ◽  
Deepa Bullappa ◽  
KR Sowmya ◽  
T Nagarathnamma
Keyword(s):  
Early Childhood ◽  
Streptococcus Mutans ◽  
Early Childhood Caries ◽  
Cross Sectional Study ◽  
Caries Experience ◽  
Sectional Study ◽  
Cross Sectional ◽  
Mothers And Children ◽  
Feeding Methods ◽  
Childhood Caries

ABSTRACT Introduction Early childhood caries (ECC) is a virulent form of dental caries that can destroy the primary dentition of toddlers and preschool children. The aim was to determine the relationship of feeding methods and oral Streptococcus mutans count in 3- to 5-year-old children with ECC. Materials and methods A cross-sectional study was conducted in children aged 3 to 5 years. Participating mothers were interviewed regarding child's demographic profile, educational level and socioeconomic status of parents, past medical and dental history of the mother and child, child's feeding habits, and dietary habits and oral hygiene practices of mother and child. Clinical examination for dental caries was done using the World Health Organization criteria (1997). Salivary samples of mother–child pair were collected to determine the pH, flow rate, and S. mutans count. Statistical tests, such as Student's t-test, analysis of variance, and Pearson's correlation were applied. Results Out of 150 mother–child pair, statistically significant difference in the caries experience was found between mothers and children with high and low S. mutans count. Moderate but statistically significant negative correlation was found between mean decayed, missing, and filled teeth of mothers and mean decayed, extracted and filled teeth (deft) of children with high S. mutans count. Regarding deft, there was no statistically significant difference between children who were exclusively breast fed (7.85 ± 2.94), exclusively bottle-fed (8.67 ± 3.98), and both breast and bottle-fed (7.77 ± 2.91). Conclusion The mean caries experience of mothers and children was 2.66 ± 2.01 and 7.82 ± 2.94 respectively, with decayed component being maximum. Moderate and significant correlation (r = 0.5) was found between S. mutans of mothers and children in saliva. Significant negative correlation was found between mothers and children with high S. mutans count (r = –0.0284; p = 0.046). How to cite this article Bullappa D, Puranik MP, Sowmya KR, Nagarathnamma T. Association of Feeding Methods and Streptococcus mutans Count with Early Childhood Caries: A Cross-sectional Study. Int J Clin Pediatr Dent 2017;10(2):119-125.

scholarly journals Firmicutes Levels in the Mouth Reflect the Gut Condition With Respect to Obesity and Early Childhood Caries

2021 ◽  
Vol 11 ◽  
Author(s):  
Karina Ferreira Rizzardi ◽  
Claudia Maria dos Santos Pereira Indiani ◽  
Renata de Oliveira Mattos-Graner ◽  
Emerson Tavares de Sousa ◽  
Marinês Nobre-dos-Santos ◽  
...  
Keyword(s):  
Early Childhood ◽  
Nutritional Status ◽  
Early Childhood Caries ◽  
Caries Experience ◽  
Pcr Analysis ◽  
Anatomical Location ◽  
Obese Children ◽  
Cross Sectional ◽  
Dental Biofilm ◽  
Childhood Caries

The present cross-sectional study investigated whether Firmicutes (F) and Bacteroidetes (B) levels in the mouth reflected the gut condition in obesity and early childhood caries (ECC). Eighty preschoolers (3-5 years) were equally assigned into four groups: 1. obese + ECC, 2. obese + caries-free (CF), 3. eutrophic + ECC, and 4. eutrophic + CF. Nutritional status and ECC were assessed based on the WHO criteria. Dental biofilm and fecal samples were collected for F and B quantification using RT-PCR analysis. Data were evaluated using three-way-ANOVA and Pearson’s correlation (α = 0.05). Regardless of the anatomical location effect (p = 0.22), there were higher values for F in the obese children + ECC compared with those in obese + caries-free (CF) in both mouth and gut (p < 0.05). The correlation for F at these sites was negative in obese children + ECC (r = −0.48; p = 0.03) and positive in obese children + CF (r=0.50; p = 0.03). Bacteroidetes were influenced by ECC (p = 0.03) and the anatomical location (p = 0.00), and the levels tended to be higher in the mouth of the obese children + ECC (p = 0.04). The F/B ratio was higher in the gut and was affected by the anatomical location (p = 0.00). This preliminary study suggested that modulated by ECC, counts of oral Firmicutes reflected corresponding condition in the gut of obese preschoolers. In addition, we first evidenced that the Firmicutes phylum behave differently according to the nutritional status and caries experience and that supragingival biofilm and gut could share levels of similarity.

Bacterial, behavioral and environmental factors associated with early childhood caries

2003 ◽  
Vol 26 (2) ◽  
pp. 165-173 ◽  
Author(s):  
Francisco Ramos-Gomez ◽  
Jane Weintraub ◽  
Stuart Gansky ◽  
Charles Hoover ◽  
John Featherstone
Keyword(s):  
Early Childhood ◽  
Environmental Factors ◽  
Early Childhood Caries ◽  
Family Income ◽  
Antibiotic Use ◽  
Mutans Streptococci ◽  
Caries Experience ◽  
Healthy Population ◽  
Cross Sectional ◽  
Childhood Caries

The goals of this cross-sectional study were to characterize and compare demographic, behavioral, and environmental factors potentially associated with early childhood caries (ECC) and to assess salivary levels of mutans streptococci (MS) and lactobacilli (LB) in underserved, predominantly Hispanic children. One hundred forty-six children aged 3 to 55 months with a range of caries experience were identified and examined. ECC was primarily associated with the presence of MS and lack of access to dental care. Salivary MS levels among young children with ECC were higher than would be expected in a dentally healthy population, but lower than levels reported among older children at high risk for caries.After adjustment for age, children with log10 MS ≥ 3.0 or log10 LB ≥ 1.5 were about five times as likely (OR=4.9, 95%CI=2.0,12.0) to have ECC than those with lower bacterial levels. This study demonstrated a significant association between relatively low cariogenic bacterial levels and dental caries in infants and toddlers. Antibiotic use, exposure to lead, and anemia were not significantly associated with the number of decayed and filled surfaces or decayed and filled teeth. ECC correlated significantly with child's age and lack of dental insurance of the children, as well as inversely with both family income and the educational level of the mother of the child.

scholarly journals Feeding Practices and Early Childhood Caries: A Cross-Sectional Study of Preschool Children in Kanpur District, India

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Santhebachalli Prakasha Shrutha ◽  
Grandim Balarama Gupta Vinit ◽  
Kolli Yada Giri ◽  
Sarwar Alam
Keyword(s):  
Early Childhood ◽  
Oral Hygiene ◽  
Early Childhood Caries ◽  
Public Health Problem ◽  
Feeding Practices ◽  
Caries Experience ◽  
Oral Health Promotion ◽  
Cross Sectional ◽  
Falling Asleep ◽  
Childhood Caries

Background. Early childhood caries (ECC) is a public health problem due to its impact on children’s health, development, and wellbeing. The objective of this study was to assess the caries experience in 3–5-year-old children and to evaluate the relationship with their mothers’ practices regarding feeding and oral hygiene habits in Kanpur. Method. A cross-sectional survey was undertaken on 2000 (974 boys and 1026 girls) children aged 3–5 years from a random sample of preschools in Kanpur district, India. Dental caries experience was recorded using WHO criteria. A pretested questionnaire with 9 questions was used for collecting information regarding mothers’ practices regarding feeding and oral hygiene practices. Chi-square test (χ2) and Student’s t-test were used for statistical analysis. Results. The prevalence of ECC was 48% with mean dmft of 2.03 ± 2.99. Boys (57%) were affected more than girls (43%) which was found to be statistically significant (P<0.05). Caries prevalence was high and statistically significant (P<0.05) among those who were breast fed for longer duration, during nighttime, those falling asleep with bottle, and those fed with additional sugar in milk. Conclusion. Determining the role of feeding practices on early childhood caries can help in the development of appropriate oral health promotion strategies.

scholarly journals Early childhood caries among premature children enrolled in WIC: A retrospective case - Controlled study

2021 ◽  
Vol 2 (2) ◽  
Author(s):  
Alison R Christensen ◽  
◽  
Fang Qian ◽  
Karin Weber-Gasparoni ◽  
◽  
...  
Keyword(s):  
Early Childhood ◽  
Logistic Regression ◽  
Oral Hygiene ◽  
Early Childhood Caries ◽  
Dietary Habits ◽  
Full Term ◽  
Caries Experience ◽  
Controlled Study ◽  
Bivariate Analysis ◽  
Childhood Caries

Purpose: To investigate the association between premature birth and Early Childhood Caries (ECC) experience, while secondarily evaluating factors that may influence development of caries. Methods: Data collected from patients enrolled at the University of Iowa’s Infant Oral Health Program consisted of prenatal history, child’s demographics, neonatal history, oral hygiene, dietary habits, and clinical findings (visible plaque, enamel defects, and caries). Bivariate and logistic regression analyses were utilized (alpha=0.05). Results: Four hundred and ninety eight randomly matched premature and full-term subjects were included (mean age=21 months [range: 6-71 months]; 51.4% males and 38.9% African Americans). Bivariate analysis revealed no significant differences existed regarding cavitated and non-cavitated caries experience between full-term and premature subjects. Additionally, compared to their counterparts, premature subjects were more likely to eat cariogenic snacks between meals (P=0.028), drink cariogenic beverages >2 times per day (P=0.027), not have their teeth brushed daily (P<0.001), and less likely to have seen a dentist (P=0.04). Logistic regression analysis showed that showed number of teeth present (OR=1.24, 95% CI: 1.10-1.39 ; P<0.001) and visible plaque on maxillary incisors (OR=5.69, 95% CI: 2.22-14.55; p<0.001) to be significantly associated with ECC in premature subjects. Conclusions: While there was no evidence that prematurity was associated with ECC, premature subjects had cariogenic dietary behaviors and high-risk oral hygiene behaviors.

scholarly journals HUBUNGAN POLA MAKAN ANAK TERHADAP TINGKAT KEJADIAN EARLY CHILDHOOD CARIES (ECC) DI KELURAHAN TANAH GARAM KOTA SOLOK

2020 ◽  
Vol 6 (1) ◽  
pp. 11-22
Author(s):  
Lala Viodita ◽  
Rizanda Machmud ◽  
Hidayati Hidayati
Keyword(s):  
Early Childhood ◽  
Significant Relationship ◽  
Early Childhood Caries ◽  
Comprehensive Treatment ◽  
Cross Sectional ◽  
Diet Pattern ◽  
Dental Diseases ◽  
Cross Sectional Design ◽  
The Relationship ◽  
Childhood Caries

Dental caries is the most commonly found for oral and dental diseases. It could appear in permanent and even desidual teeth. One of the oral  and dental diseases prevalent to children of age 1-5 years old is termed Early Childhood Caries (ECC) which was an acute dental infection that rapidly develop from one third of primary servical maxillary incisivus into the whole teeth making white to yellowish cavity with soft caries tissue. Diet pattern gives big influence on incidence of caries. to find the relationship between children diet pattern with the case of ECC. observational analytical using cross- sectional design. Sample size was 50 children of age 2-3 years old in three different Comprehensive Treatment Post (CTP) in working area of Tanah Garam primary healthcare in Solok City. ECC prevalence in Tanah Garam District of Solok City in 2018 was 56 %. Diet pattern found mostly was cariogenic for 52 %. There was significant relationship between diet pattern with the case of ECC in Tanah Garam District of Solok City with p = 0.01. There was significant relationship between diet pattern with the case of ECC in Tanah Garam District Solok City.

scholarly journals Metabolomics Insights in Early Childhood Caries

2021 ◽  
pp. 002203452098296
Author(s):  
L.H. Heimisdottir ◽  
B.M. Lin ◽  
H. Cho ◽  
A. Orlenko ◽  
A.A. Ribeiro ◽  
...  
Keyword(s):  
Early Childhood ◽  
Multiple Testing ◽  
Early Childhood Caries ◽  
Case Definition ◽  
Caries Detection ◽  
Tooth Surface ◽  
Caries Experience ◽  
Multiple Testing Correction ◽  
Cross Sectional ◽  
Childhood Caries

Dental caries is characterized by a dysbiotic shift at the biofilm–tooth surface interface, yet comprehensive biochemical characterizations of the biofilm are scant. We used metabolomics to identify biochemical features of the supragingival biofilm associated with early childhood caries (ECC) prevalence and severity. The study’s analytical sample comprised 289 children ages 3 to 5 (51% with ECC) who attended public preschools in North Carolina and were enrolled in a community-based cross-sectional study of early childhood oral health. Clinical examinations were conducted by calibrated examiners in community locations using International Caries Detection and Classification System (ICDAS) criteria. Supragingival plaque collected from the facial/buccal surfaces of all primary teeth in the upper-left quadrant was analyzed using ultra-performance liquid chromatography–tandem mass spectrometry. Associations between individual metabolites and 18 clinical traits (based on different ECC definitions and sets of tooth surfaces) were quantified using Brownian distance correlations (dCor) and linear regression modeling of log2-transformed values, applying a false discovery rate multiple testing correction. A tree-based pipeline optimization tool (TPOT)–machine learning process was used to identify the best-fitting ECC classification metabolite model. There were 503 named metabolites identified, including microbial, host, and exogenous biochemicals. Most significant ECC-metabolite associations were positive (i.e., upregulations/enrichments). The localized ECC case definition (ICDAS ≥1 caries experience within the surfaces from which plaque was collected) had the strongest correlation with the metabolome (dCor P = 8 × 10−3). Sixteen metabolites were significantly associated with ECC after multiple testing correction, including fucose ( P = 3.0 × 10−6) and N-acetylneuraminate (p = 6.8 × 10−6) with higher ECC prevalence, as well as catechin ( P = 4.7 × 10−6) and epicatechin ( P = 2.9 × 10−6) with lower. Catechin, epicatechin, imidazole propionate, fucose, 9,10-DiHOME, and N-acetylneuraminate were among the top 15 metabolites in terms of ECC classification importance in the automated TPOT model. These supragingival biofilm metabolite findings provide novel insights in ECC biology and can serve as the basis for the development of measures of disease activity or risk assessment.

scholarly journals Feeding practices and early childhood caries among children with primary dentition

2021 ◽  
Vol 10 (2) ◽  
pp. 74-79
Author(s):  
Sunanda Sundas ◽  
Barun Kumar Sah ◽  
Neha Dhakal ◽  
Amita Rai ◽  
Neera Joshi
Keyword(s):  
Early Childhood ◽  
Early Childhood Caries ◽  
Feeding Practices ◽  
Bottle Feeding ◽  
Caries Experience ◽  
Cross Sectional ◽  
Validated Questionnaire ◽  
Night Feeding ◽  
Significant Difference ◽  
Childhood Caries

Background: Early childhood caries (ECC) is major oral health problem of young children. Risk factors for ECC are poor oral hygiene and feeding practices. Prevalence and severity of ECC is increasing with change in lifestyle and diet pattern in developing countries. Objectives: To assess the association between feeding practices and severity of early childhood caries in preschool children. Methods: An analytical cross-sectional study was conducted with convenient sample of 96 children up to six years age in Peoples Dental College from July to December 2020. Clinical examination included the recording of dental caries (dmft) using WHO criteria. The self-validated questionnaire consisting seven questions about feeding practices was asked to mothers. The Chi-square test was performed to determine association between severity of ECC and feeding pattern. Results: Mean dmft was 6.77 ± 5.91. Prevalence of severe-ECC (S-ECC) was highest in youngest age group. Caries experience was similar in breast fed, bottle fed, and mixed. There was no significant difference in non-severe ECC and ECC in relation to duration of breast or bottle feeding but frequency of night feeding was associated with S-ECC. Children given ready-made infant formula solid food had more S-ECC (13, 92%) compared to non-severe ECC (1, 7.1%) which was statistically significant. Children given homemade gram flour food were noted to have less of S-ECC (5, 27.5%) than nonsevere ECC (13, 72.5%) which was statistically significant. Conclusion: Present study revealed that multiple night feeding and weaning with readymade food are significant factors for S-ECC.

scholarly journals Trends in caries experience and background factors in 3-year-old children in Poland: evidence from epidemiological surveys during 2002-2017

2019 ◽  
Vol 82 (1) ◽  
pp. 79-90
Author(s):  
Anna Turska-Szybka ◽  
Urszula Kaczmarek ◽  
Dariusz Gozdowski ◽  
Jacek Tomczyk ◽  
Dorota Olczak-Kowalczyk
Keyword(s):  
Early Childhood ◽  
Dental Caries ◽  
Oral Hygiene ◽  
Early Childhood Caries ◽  
Dietary Habits ◽  
Caries Experience ◽  
Fluoride Toothpaste ◽  
Behavioural Changes ◽  
Sugary Beverages ◽  
Childhood Caries

Abstract The prevalence of early childhood caries and its level varies. The present study was to establish the trends in dental caries and the impact of behavioural changes on the prevalence of caries in three-yearolds in Poland within a fifteen-year period. The results of a cross sectional survey carried out on 3439 three-year-olds in 2002, 2009, and 2017 using WHO criteria for dental caries (dmft, dmft=0, dmft≥4) and the results of a questionnaire filled by their parents with data on sociodemographics, oral hygiene and dietary habits, especially their sugar intake, were assessed. The dmft/dmfs index is applied to the primary dentition and is expressed as the total number of teeth/surfaces that are decayed, missing, or filled. The Cochran-Armitage test for trend was used to assess the fraction changes in time. The Pearson correlation coefficient was used to assess the changes in dmft trends and the correlations between behavioural changes, awareness levels, and the prevalence of caries. Within the fifteen-year period minimal changes in the prevalence of early childhood caries (15% down), dmft≥4 (11.4% down) and a lower dmft (36% down) were accompanied by a better parent awareness about the causes of caries and better oral hygiene routines. Sugary beverages were no longer drank at least once a day, however sweetened milk, cake, doughnuts, and sweet rolls were consumed more often. Being female, living in an urban area, having parents more aware about caries, consuming sugary beverages less frequently, brushing teeth twice a day, and using a fluoride toothpaste promoted lower early childhood caries. Gradually healthier teeth are linked to an increased awareness of the parents and healthier routines. A too frequent exposure to sugar promotes early childhood caries. Should the changes of dietary habits be insufficient, brushing teeth with fluoride toothpaste becomes crucial.

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