The Protective Effect of Pre-Moxibustion on Reproductive Hormones Profile of Rats with Tripterygium Glycosides-Induced Ovarian Damage

2020 ◽  
Vol 27 (6) ◽  
pp. 401-409
Author(s):  
Shipeng Zhu ◽  
Yaoshuai Wang ◽  
Xiaoxia Chang ◽  
Huan Chen ◽  
Xun Jin
Keyword(s):  
Protective Effect ◽  
Estrous Cycle ◽  
Ovarian Reserve ◽  
Follicular Development ◽  
Control Model ◽  
Reproductive Hormones ◽  
Female Rats ◽  
Estrous Cycles ◽  
Time Points ◽  
Ovarian Damage

<b><i>Background:</i></b> Acupuncture and moxibustion have been proven to be conducive to improving the ovarian reserve. However, the mechanism of pre-moxibustion on <i>Tripterygium</i> glycosides (TG)-induced ovarian damage has not been previously reported. <b><i>Method:</i></b> Female rats were randomly divided into 5 groups: control, model (75 mg/kg TG, 14 days), preventive moxibustion 1 (PM1, receiving moxibustion for 4 weeks before TG administration), preventive moxibustion 2 (PM2, receiving moxibustion for 2 weeks before TG administration and another 2 weeks during TG administration), and preventive moxibustion 3 (PM3, receiving 4 weeks of moxibustion during TG administration). The estrous cycle of the animal was recorded after TG administration. Rats were sacrificed 14 days after TG administration. The reproductive hormones profiles in serum, ovary, and hypothalamic tissues were analyzed. <b><i>Result:</i></b> Pre-moxibustion could revert abnormal estrous cycles, relieve follicle damage, and improve abnormal secretion of reproductive hormones resulting from ovarian damage. However, both PM2 and PM3 were more effective than PM1. In addition, PM2 disclosed more advantages in regulating reproductive hormones abnormalities, while PM3 performed better in follicular development. <b><i>Conclusion:</i></b> In combination, the findings of this study suggest that pre-moxibustion is effective in protecting the ovary from damage in TG-induced ovarian damage rats. But different time points correspond to different modulation targets and mechanisms.

Splitting of the locomotor activity rhythm in rats by exposure to continuous light

1983 ◽  
Vol 244 (4) ◽  
pp. R573-R576 ◽  
Author(s):  
Phyllis W. Cheung ◽  
Charles E. McCormack
Keyword(s):  
Locomotor Activity ◽  
Estrous Cycle ◽  
Activity Rhythm ◽  
Continuous Light ◽  
Female Rats ◽  
Locomotor Rhythm ◽  
Estrous Cycles ◽  
Locomotor Activity Rhythm ◽  
Active Portion ◽  
Free Running

Female rats exposed to low intensities (0.1–1.5 lx) of continuous light (LL), displayed regular estrous cycles and free-running circadian rhythms of locomotor activity. In most rats, as the intensity of LL was increased to >2.0 lx, components within the active portion (α) of the locomotor rhythm remained synchronized as the periodicity of the rhythm lengthened. However, in a few rats agr split into two components; one of which free-ran with a period shorter than 24 h, while the other free-ran with a period longer than 24 h. As soon as the two components became maximally separated they spontaneously rejoined. In most rats, estrous cycles ceased shortly after the intensity of LL was increased to >2.0 lx even though the locomotor activity rhythm retained its unsplit free-running nature. These observations suggest that the multiple oscillators that control the rhythms of locomotor activity and the estrous cycle are normally coupled to one another. In certain intensities of LL, these oscillators uncouple and free-run with different periodicities, a condition which causes estrous cycles to cease and sometimes produces a split locomotor activity rhythm. circadian rhythm; oscillators; estrous cycle Submitted on November 9, 1981 Accepted on October 11, 1982

scholarly journals The protective effect of platelet-rich plasma administrated on ovarian function in female rats with Cy-induced ovarian damage

2020 ◽  
Vol 37 (4) ◽  
pp. 865-873 ◽  
Author(s):  
Pinar Ozcan ◽  
Taha Takmaz ◽  
Olgu Enis Tok ◽  
Sevde Islek ◽  
Esra Nur Yigit ◽  
...  
Keyword(s):  
Protective Effect ◽  
Ovarian Function ◽  
Platelet Rich Plasma ◽  
Female Rats ◽  
Ovarian Damage

Cardiac contraction, calcium transients, and myofilament calcium sensitivity fluctuate with the estrous cycle in young adult female mice

2014 ◽  
Vol 306 (7) ◽  
pp. H938-H953 ◽  
Author(s):  
Jennifer K. MacDonald ◽  
W. Glen Pyle ◽  
Cristine J. Reitz ◽  
Susan E. Howlett
Keyword(s):  
Estrous Cycle ◽  
Action Potentials ◽  
Ventricular Myocytes ◽  
Fold Increase ◽  
Reproductive Hormones ◽  
Cardiac Contraction ◽  
Estrous Cycles ◽  
Female Mice ◽  
Ec Coupling ◽  
The Impact

This study established conditions to induce regular estrous cycles in female C57BL/6J mice and investigated the impact of the estrous cycle on contractions, Ca2+ transients, and underlying cardiac excitation-contraction (EC)-coupling mechanisms. Daily vaginal smears from group-housed virgin female mice were stained to distinguish estrous stage (proestrus, estrus, metestrus, diestrus). Ventricular myocytes were isolated from anesthetized mice. Contractions and Ca2+ transients were measured simultaneously (4 Hz, 37°C). Interestingly, mice did not exhibit regular cycles unless they were exposed to male pheromones in bedding added to their cages. Field-stimulated myocytes from mice in estrus had larger contractions (∼2-fold increase), larger Ca2+ transients (∼1.11-fold increase), and longer action potentials (>2-fold increase) compared with other stages. Larger contractions and Ca2+ transients were not observed in estrus myocytes voltage-clamped with shorter action potentials. Voltage-clamp experiments also demonstrated that estrous stage had no effect on Ca2+ current, EC-coupling gain, diastolic Ca2+, sarcoplasmic reticulum (SR) Ca2+ content, or fractional release. Although contractions were largest in estrus, myofilament Ca2+ sensitivity was lowest (EC50 values ∼1.15-fold higher) in conjunction with increased phosphorylation of myosin binding protein C in estrus. Contractions were enhanced in ventricular myocytes from mice in estrus because action potential prolongation increased SR Ca2+ release. These findings demonstrate that cyclical changes in reproductive hormones associated with the estrous cycle can influence myocardial electrical and contractile function and modify Ca2+ homeostasis. However, such changes are unlikely to occur in female mice housed in groups under conventional conditions, since these mice do not exhibit regular estrous cycles.

scholarly journals Pengendalian Folikulogenesis Ovarium dengan Pemberian Ekstrak Biji Kapas

2017 ◽  
Vol 35 (1) ◽  
pp. 71
Author(s):  
Siska Adelya Ramadhani ◽  
Iman Supriatna ◽  
Ni Wayan Kurniani Karja ◽  
Adi Winarto
Keyword(s):  
Body Weight ◽  
Estrous Cycle ◽  
Treatment Period ◽  
Cotton Seed ◽  
Follicular Development ◽  
Giemsa Staining ◽  
Vaginal Smear ◽  
Estrous Cycles ◽  
Dose Treatment ◽  
Female Mice

Gosipol is a substances contained in extracted cotton seed which is thought to have the antifertility ability therefore it is often used as a herbal contraceptive. The aim of this study were to assess the folliculogenesis in mice after administrated with cottonseed extract. 60 female mice strain DDY which was 14-15 weeks old and 30-35 g body weight were divided into five groups and given cottonseed extract each 0; 1,5; 2,1 and 2,7 g/kg BW for 5, 10, 15, 24, and 24 + 10 days (without cottonseed treatment). At the end of the treatment period, mice was euthanasia to observe follicular development histomorphology (each three mice of each treatment). Mice estrous status were evaluated based on the description of the vaginal smear cells with Giemsa staining. The results showedthat the number of developing follicles was low (P < 0.05) compared with control after 5 days cottonseed extract administration at dose 2,7 g/kg BW that were 23 ± 3,6. At dose 1,5 and 2,1 g/kg BW the number of follicles was low after 24 days that were 25 ± 10,4 and 27 ± 3,5. Recovery effects of follicle number after cottonseed extract administration for 24 days was the best at a dose of 1,5 g/kg BW. Prolonge of estrous cycle occured in mice which were administrated the cottonseed extract of at all dose treatment. In conclusion, although the decrease in the number of developing follicles and prolonge of estrous cycles occurred after cottonseed extract administration, but these effects are reversible after the administration ended.

Melatonin Effect during Aging on Reproductive Hormones of Female Rats through the Estrous Cycle

2005 ◽  
pp. 333-336
Author(s):  
B. Díaz ◽  
E. Díaz ◽  
C. Fernindez ◽  
P. O. Castrillón ◽  
A. I. Esquifino ◽  
...  
Keyword(s):  
Estrous Cycle ◽  
Reproductive Hormones ◽  
Female Rats

scholarly journals Neonatal handling and reproductive function in female rats

2005 ◽  
Vol 184 (2) ◽  
pp. 435-445 ◽  
Author(s):  
C M Gomes ◽  
C Raineki ◽  
P Ramos de Paula ◽  
G S Severino ◽  
C V V Helena ◽  
...  
Keyword(s):  
Estrous Cycle ◽  
Plasma Levels ◽  
Preoptic Area ◽  
Reproductive Function ◽  
Female Rats ◽  
Estrous Cycles ◽  
Neonatal Handling ◽  
Environmental Stimulation ◽  
Medial Septal Area ◽  
Lh Releasing Hormone

Neonatal handling induces anovulatory estrous cycles and decreases sexual receptivity in female rats. The synchronous secretion of hormones from the gonads (estradiol (E2) and progesterone (P)), pituitary (luteinizing (LH) and follicle-stimulating (FSH) hormones) and hypothalamus (LH-releasing hormone (LHRH)) are essential for the reproductive functions in female rats. The present study aimed to describe the plasma levels of E2 and P throughout the estrous cycle and LH, FSH and prolactin (PRL) in the afternoon of the proestrus, and the LHRH content in the medial preoptic area (MPOA), median eminence (ME) and medial septal area (MSA) in the proestrus, in the neonatal handled rats. Wistar pup rats were handled for 1 min during the first 10 days after delivery (neonatal handled group) or left undisturbed (nonhandled group). When they reached adulthood, blood samples were collected through a jugular cannula and the MPOA, ME and MSA were microdissected. Plasma levels of the hormones and the content of LHRH were determined by RIA. The number of oocytes counted in the morning of the estrus day in the handled rats was significantly lower than in the nonhandled ones. Neonatal handling reduces E2 levels only on the proestrus day while P levels decreased in metestrus and estrus. Handled females also showed reduced plasma levels of LH, FSH and PRL in the afternoon of the proestrus. The LHRH content in the MPOA was significantly higher than in the nonhandled group. The reduced secretion of E2, LH, FSH and LHRH on the proestrus day may explain the anovulatory estrous cycle in neonatal handled rats. The reduced secretion of PRL in the proestrus may be related to the decreased sexual receptiveness in handled females. In conclusion, early-life environmental stimulation can induce long-lasting effects on the hypothalamus-pituitary-gonad axis.

scholarly journals Excitatory and inhibitory influence of exogenous neurotransmitters on reproduction in female rats

2018 ◽  
Vol 2 (3) ◽  
pp. 286-298
Author(s):  
Hassan Ahmed ◽  
Fatma Ali ◽  
Ahmed E. Ahmed ◽  
Ahmed I. Ahmed ◽  
Eid Abdelhameed
Keyword(s):  
Nervous System ◽  
Histopathological Examination ◽  
Ovarian Tissue ◽  
Reproductive Hormones ◽  
Female Rats ◽  
Corpora Lutea ◽  
Inhibitory Influence ◽  
Estrous Cycles ◽  
Vacuolated Cytoplasm

Neurotransmitters are mediators inside the nervous system responsible for transmitting neural-neural or neural-organs signals. Several neural studies have tried to unveil the role of such mediators whose action extended outside the nervous system such as immune, digestive, circulatory and reproductive systems. The present study aimed to investigate the role of the excitatory-glutamate and inhibitory-GABA transmitters in female rats reproduction including their effects on gonadotropins; luteinizing hormone (LH) and follicle stimulating hormone (FSH) and the sex steroids; estrogen (E2) and progesterone (P4) during the estrous cycle as well. Furthermore, the responsive changes on the ovarian tissue were also studied. Synthetic glutamate and GABA were injected intraperitoneally (ip) in those animals throughout four successive estrous cycles. Interestingly, the ip injections of glutamate increased the levels of LH, E2 and P4 but decreased those of FSH significantly. However, the ip injections of GABA significantly decreased the levels of LH in the 4th cycles and FSH throughout treatment period while it increased the levels of E2 and P4. All changes occurred in those reproductive hormones caused by glutamate has been recovered after cessation of glutamate and GABA injection except FSH, including; the 5th, 6th and 7th cycles. Regarding to histopathological examination, ovaries of treated rats showed deleterious changes. The glutamate-treated rats ovaries showed atrophy of the primary follicles with degenerative changes in those secondary and tertiary follicles with obvious degeneration in the granulosa cell layer with vacuolated cytoplasm. On the other hand, those received GABA showed degeneration of the oocytes with congestion of blood vessels supplying the corpora lutea (CL) associated with endothelial changes. The histopathological changes in CL have been improved after glutamate cessation while not changed after GABA cessation.

Morphological Changes in the Rat Granulosa Cell Surface During Differentiation Induced by Estradiol and Gonadotropins

1977 ◽  
Vol 35 ◽  
pp. 484-485
Author(s):  
P. Bagavandoss ◽  
JoAnne S. Richards ◽  
A. Rees Midgley
Keyword(s):  
Cell Surface ◽  
Granulosa Cell ◽  
Morphological Changes ◽  
Follicular Development ◽  
Female Rats ◽  
Functional Changes ◽  
Group 4 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2

During follicular development in the mammalian ovary, several functional changes occur in the granulosa cells in response to steroid hormones and gonadotropins (1,2). In particular, marked changes in the content of membrane-associated receptors for the gonadotropins have been observed (1).We report here scanning electron microscope observations of morphological changes that occur on the granulosa cell surface in response to the administration of estradiol, human follicle stimulating hormone (hFSH), and human chorionic gonadotropin (hCG).Immature female rats that were hypophysectcmized on day 24 of age were treated in the following manner. Group 1: control groups were injected once a day with 0.1 ml phosphate buffered saline (PBS) for 3 days; group 2: estradiol (1.5 mg/0.2 ml propylene glycol) once a day for 3 days; group 3: estradiol for 3 days followed by 2 days of hFSH (1 μg/0.1 ml) twice daily, group 4: same as in group 3; group 5: same as in group 3 with a final injection of hCG (5 IU/0.1 ml) on the fifth day.

Sign in / Sign up

Export Citation Format

Share Document