scholarly journals Effect of silver nanoparticles, synthesized from fleshy stem and leaves extract of Brassica campestrisL., on Alternari lini inhibition

2020 ◽  
Vol 1 (2) ◽  
pp. 86-91
Author(s):  
Syed Awais Hussain Shah
Keyword(s):  
Silver Nanoparticles ◽  
Field Emission ◽  
Fungal Pathogens ◽  
Research Work ◽  
Colour Change ◽  
Pathogenic Fungi ◽  
Significant Inhibition ◽  
Diffusion Method ◽  
Linum Usitatissimum L ◽  
Uv Visible

Several fungal pathogens showed resistance against pesticides, plant mediated synthesized silver nanoparticles have been recognized as effective against them. The present research work was an attempt to synthesize silver nanoparticles by using fleshy stem and leaves ex-tract of Brassica compestris L. from silver nitrate, which reduces it to synthesized silver nanoparticles. Biosynthesized AgNP’s were char-acterized by different techniques like UV-visible spectrophotometry and Field Emission Scanning Electron Microscopy and fungal inhibi-tory activity by well diffusion method. The colour change of solution indicated the synthesis of silver nanoparticles. UV-visible spectro-photometer showed the peak between 415 to 455 nm for fleshy stem and leaves extract of B. compestris and Field Emission Scanning Elec-tron Microscope analysis revealed that spherical shaped nanoparti-cles with size 20-65 nm. These synthesized nanoparticles showed in-hibitory activity against fungi, Alternaria lini, a linseed blight patho-gen which causes linseed blight disease in Linum usitatissimum L. The 30 μl concentrations of silver nanoparticles had showed significant inhibition to growth of A. lini. These findings may suggest AgNP’s syn-thesized by B. compestris used against plant pathogenic fungi.

scholarly journals OPTIMIZATION OF GREEN SYNTHESIZED SILVER NANOPARTICLES FROM CARALLUMA UMBELLATA

2018 ◽  
Vol 10 (4) ◽  
pp. 103 ◽  
Author(s):  
Anju K ◽  
Anitha Jegadeeshwari L ◽  
Nagendra Gandhi N
Keyword(s):  
Silver Nanoparticles ◽  
Antimicrobial Agent ◽  
Colour Change ◽  
Diffusion Method ◽  
Plant Sample ◽  
Optimum Conditions ◽  
X Ray ◽  
Stem Extract ◽  
Uv Visible ◽  
Antibacterial And Antifungal

Objective: The current study focuses on optimization and ecologically innocuous green synthesis of silver nanoparticles (AgNPs) using tribal plant Caralluma umbellata and to study its potential as an antibacterial and antifungal agent.Methods: The synthesis of AgNPs were confirmed by the colour change of the stem extract from yellow to dark brown and by UV-Visible spectroscopy. The optimum conditions for synthesis of AgNPs were analysed using Response surface methodology (RSM) based Box-Behnken design (BBD) using Design Expert software (7.0.0 trial version). The AgNPs synthesized were characterized by Scanning electron microscope (SEM), Energy dispersive X-ray (EDX), Fourier transform infrared (FTIR) and X-ray diffraction (XRD) analysis. Further, antibacterial and antifungal activity were performed using well diffusion method for both plant extract and AgNPs.Results: The UV-Visible spectrum of AgNPs revealed characteristic peak at 425.5 nm. The crystalline nature of synthesized AgNPs was confirmed by XRD with average size 26 nm. SEM confirms the spherical shape of AgNPs and by EDX the presence of elemental silver was observed. The ability of the plant to produce both reducing and capping agents were confirmed by FTIR. The optimum conditions for synthesis of AgNPs were found to be 0.55 mmol AgNO3 concentration, 45 °C temperature and 24h reaction time. Both plant sample and synthesized AgNPs exhibited good antimicrobial activity where AgNPs showed superior efficacy as an antimicrobial agent over the other.Conclusion: From the results obtained, it can be deduced that both C. umbellata stem extract and synthesized AgNPs can act as potent antimicrobial agent. But the synthesized AgNPs is more potent against bacteria and fungus.

scholarly journals Antibacterial Activity of Silver Nanoparticles Synthesized by Bark Extract of Syzygium cumini

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Ram Prasad ◽  
Vyshnava Satyanarayana Swamy
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Cost Effective ◽  
Diffusion Method ◽  
Bark Extract ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cost Effective Method ◽  
Uv Visible ◽  
Major Attention

The unique property of the silver nanoparticles having the antimicrobial activity drags the major attention towards the present nanotechnology. The environmentally nontoxic, ecofriendly, and cost-effective method that has been developed for the synthesis of silver nanoparticles using plant extracts creates the major research interest in the field of nanobiotechnology. The synthesized silver nanoparticles have been characterized by the UV-visible spectroscopy, atomic force microscopy (AFM), and scanning electron microscopy (SEM). Further, the antibacterial activity of silver nanoparticles was evaluated by well diffusion method, and it was found that the biogenic silver nanoparticles have antibacterial activity against Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 29213), Pseudomonas aeruginosa (ATCC 27853), Azotobacter chroococcum WR 9, and Bacillus licheniformis (MTCC 9555).

scholarly journals Green Synthesis of Silver Nanoparticles from the Extracts of Fruit Peel of Citrus tangerina, Citrus sinensis, and Citrus limon for Antibacterial Activities

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Moira Carmalita Dharsika Niluxsshun ◽  
Koneswaran Masilamani ◽  
Umaramani Mathiventhan
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Citrus Sinensis ◽  
Orange Peel ◽  
Cost Effective ◽  
Diffusion Method ◽  
Citrus Limon ◽  
Fruit Peel ◽  
Visible Absorption ◽  
Uv Visible

Wide application of nanoparticles motivates the need for synthesising them. Here, a nontoxic, eco-friendly, and cost-effective method has been established for the synthesis of silver nanoparticles using extracts of lemon peel (Citrus limon), green orange peel (Citrus sinensis), and orange peel (Citrus tangerina). The synthesised nanoparticles have been characterised using UV-visible absorptionspectroscopy, Fourier transform infrared spectroscopy, and transmission electron microscopy (TEM). The UV-visible absorption spectrum of these synthesised silver nanoparticles shows an absorption peak at around 440 nm. TEM images show different shaped particles with various sizes. Furthermore, the antibacterial activity of silver nanoparticles was appraised by a well-diffusion method and it was observed that the green synthesised silver nanoparticles have an effective antibacterial activity against Escherichia coli and Staphylococcus aureus. The outcome of this study could be beneficial for nanotechnology-based biomedical applications.

scholarly journals A comparative evaluation of Kumaun Himalayan Gymnosperms for their Antifungal potential against plant pathogenic fungi

2018 ◽  
Vol 7 (3) ◽  
pp. 230-241
Author(s):  
Savita Joshi ◽  
◽  
Parikshit Kumar ◽  
Prabha Pant ◽  
SC Sati ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fungal Pathogens ◽  
Ethanol Extract ◽  
Pathogenic Fungi ◽  
Total Activity ◽  
Maximum Activity ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Cedrus Deodara ◽  
Araucaria Cunninghamii

Fungicidal activity of 10 ethnobotanically known Kumaun Himalayan gymnospermous plants namely Araucaria cunninghamii, Biota orientalis, Cedrus deodara, Cephalotaxus griffithi, Cryptomeria japonica Cupressus torulosa, Ginkgo biloba, Juniperus communis, Picea smithiana and Pinus wallichiana were tested against six plant disease causing fungal pathogens by agar well-diffusion method. Forty extracts of these gymnospermic leaves in different organic solvents (methanol, ethanol, chloroform and hexane) were studied by performing the 160 sets of experiments. The MIC values of each extract (where % inhibition ≥ 40%) were also determined. All the plant extracts exhibited strong antifungal activity. Results indicated that all leaves extracts of C. griffithi and G. biloba were found most effective among the tested plants extracts. Hexane extract of C. griffithi was showed highest inhibitory activity against C. falcatum (72%; MIC, 7.81µg/ml) and T. indica (70%; MIC, 15.62µg/ml). On the other hand, ethanol extract of G. biloba also showed remarkable activity against P. oryzae (66% with MIC, 7.81g/ml). While P. wallichiana leave extracts were found less active among the studied plants against all the tested fungal strains. The chloroform extracts were found the most effective against all the tested fungi (10% to 60%), followed by ethanol extract (30-50%), methanol extract (20-40%), while in hexane extracts ranged 10-30% only. The extracts of C. griffithi exhibited superior Relative Antifungal Activity (RAA, 20%), followed by G. biloba and A. cunninghamii (RAA, 19 and 12%, respectively). All data were also analyzed for determination of total activity of plant for each studied species of gymnosperm. C. griffithi had maximum activity i.e. 71 % followed by G. biloba (54%) and A. cunninghamii (33%). C. torulosa showed the least total activity and RAA i.e. 8% and 3%, respectively. All the plant species assayed possess definite antifungal properties and suggested for phytochemical analysis to identify the active principles responsible for their antifungal activity

scholarly journals Biosynthesis and Characterisation of Silver Nanoparticles using Leaf Extract of Achras sapota l. for its Antimicrobial Activity

2020 ◽  
pp. 246-256
Author(s):  
Maphibanri Maring ◽  
Akila Elias ◽  
V. B. Narayanaswamy
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Colour Change ◽  
Visible Spectroscopy ◽  
Sem And Tem ◽  
Achras Sapota ◽  
Uv Visible ◽  
Biosynthesized Agnps ◽  
Ethanolic Leaf Extract

<p>Nanotechnology is a field that is rapidly growing, making an impact in all spheres of human life. In the current study, silver nanoparticles were synthesized using the ethanolic leaf extract of <em>Achras sapota. </em>Characterization was carried out using UV-Visible spectroscopy, FTIR, XRD, SEM and TEM. The formation of AgNPs was confirmed through UV-Visible spectroscopy by the colour change. Based on the XRD pattern, the crystalline property of the AgNPs was established. The functional group present in the ethanolic leaf extract of <em>Achras sapota</em> is responsible for the reduction of the Ag<sup>+</sup> ion which was studied through FTIR. From the SEM and TEM analysis, it was found that the formed nanoparticles are spherical in shape and nano in size. The biosynthesized AgNPs was evaluated for its antimicrobial activity against gram positive bacteria (<em>S. aureus and L. bacillus</em>) and gram negative bacteria (<em>E. coli and P. aeruginosa</em>) using disc diffusion for preliminary screening of antimicrobial activity and dilution method for evaluation of antibacterial effectiveness and effect of silver nanoparticles on bacterial growth and it was found to exhibit potential antimicrobial activity. The biosynthesized AgNPs was found to be efficient in terms of reaction time as well as stability, eco-friendly and cost effective.</p>

scholarly journals Biosynthesis and characterization of silver nanoparticles using ginger spent and their antibacterial activity

2021 ◽  
pp. 174-179
Author(s):  
Sinthia Ganesan ◽  
Palanichamy Mehalingam ◽  
Govindan Sadasivam Selvam
Keyword(s):  
Silver Nanoparticles ◽  
Waste Product ◽  
Colour Change ◽  
Zingiber Officinale ◽  
Xrd Analysis ◽  
Sem Analysis ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Gram Positive Bacteria ◽  
Vis Spectroscopy

Ginger spent is the byproduct of spice industries that remove the essential oils of ginger (Zingiber officinale) for food industry and medicinal purposes. Ginger is a well known spice used often for seasoning in Indian cuisine. The de-oiled ginger has no specific use mostly goes to waste. Hence, we utilized this industrial waste product in the efficient synthesis of silver nanoparticles with the aid of UV irradiation from a solution of 1mM silver nitrate and spent extract in the ratio 9:1. Immediate colour change from pale yellow to dark brown was noted indicating the rapid synthesis of silver nanoparticles. These nanoparticles were centrifuged, dried and well characterized. UV Vis Spectroscopy, XRD analysis, Zeta potential and SEM analysis was carried out. It was commendable that the size of the nanoparticles fell well within the upper limit of 100nm. Agar well diffusion method was used to screen the antimicrobial activity of the well characterized silver nanoparticles. They were tested against seven pathogenic strains of three gram negative bacteria (Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa) three gram positive bacteria(Bacillus subtilis, Staphylococcus aureus and Streptococcus faecalis) and a fungus (Candida  albicans). It was seen that the zone of inhibition(ZOI) in well plate method  increased on increasing the concentration of silver nanoparticles. Further studies could lead to the application of these silver nanoparticles in medicine.

Isolation and Molecular Characterisation of Ginger (Zingiber officinale. rosc.) Soft Rot Pathogenic Fungi from Champhai District of Mizoram, India

2021 ◽  
Vol 9 (2) ◽  
pp. 46-53
Author(s):  
Albana L. Chawngthu ◽  
◽  
G. Rosangkima ◽  
Vanram liana ◽  
Chhungpuii Khawlhring ◽  
...  
Keyword(s):  
Fungal Pathogens ◽  
Research Work ◽  
Morphological Characteristics ◽  
Pathogenic Fungi ◽  
Zingiber Officinale ◽  
Soft Rot ◽  
Therapeutic Uses ◽  
Causative Agents ◽  
Rot Disease ◽  
Internal Transcribed Spacer Rdna

Ginger (Zingiber officinale Rosc.,Zingiberaceae) is an important crop grown in India known for its therapeutic uses. Wilting caused by bacteria, soft rot and yellow disease caused by fungi are the major diseases affecting Ginger production and its cultivation hampering its growth and development. The current study emphasize on the morphological and molecular identification of fungal pathogens causing soft rot disease in ginger that have become one of the major problem among farmers of Mizoram, India as a result of which resulted in a huge decline in rhizome yield and its production. Nevertheless, due to limited studies of the medicinal plants the causative agents associated with these plants are not available. The objective of this study is to investigate the diversity of fungi that cause soft rot in ginger. Twenty fungi were successfully isolated from four villages of Champha iDistrict, Mizoram. The fungi isolated from the infected rhizome were cultured and identification was carried out using morphological characteristics and molecular analysis of DNA sequence generated from Internal Transcribed Spacer rDNA region. Of the samples collected, F. solani,F. oxysporum, P. myriotylum were the most common causative agents. Much research work has yet to be undertaken explore the diversity of pathogrnic fungi causing soft rot in Mizoram which could be very significant in introducing competent and eco-friendly disease management programme.

scholarly journals PLANT-MEDIATED ZNO NANOPARTICLES USING FICUS RACEMOSA LEAF EXTRACT AND THEIR CHARACTERIZATION, ANTIBACTERIAL ACTIVITY

2018 ◽  
Vol 11 (9) ◽  
pp. 463
Author(s):  
Arun Babu Birusanti ◽  
Umamahesh Mallavarapu ◽  
Devanna Nayakanti ◽  
Chandra Sekhar Espenti
Keyword(s):  
Antibacterial Activity ◽  
Leaf Extract ◽  
Research Work ◽  
Blue Emission ◽  
Hexagonal Structure ◽  
Diffusion Method ◽  
X Ray ◽  
Average Grain Size ◽  
Uv Visible ◽  
Ficus Racemosa

Objective: The motto of this research work was to synthesize the zinc oxide nanoparticles (ZnONPs) should be environmental friendly. Hence, it receives more attention toward the green route method.Methods: At last, the Ficus racemosa ZnONPs (FR-ZnONPs) were successfully synthesized using a simple protocol and eco favorable technique. This paper highlights the biosynthesis of ZnONPs using leaf extract of F. racemosa. Results: FR-ZnONPs formation was confirmed by the different spectral analysis such as UV-visible spectroscopy, Fourier transform-infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM), and electronic dispersive X-ray spectroscopy. UV-visible studies revealed that the intrinsic band gap absorptions were at 372 nm and photoluminescence study showed that the blue emission at 492, 481, 473, and 450 nm and the green emission at 540 nm, respectively. FR-ZnONPs are wurtzite hexagonal structure with an average grain size of 15 nm was found from XRD analysis.Conclusion: FR-ZnONPs exhibited good antimicrobial efficacy on Escherichia coli and Staphylococcus aureus with various concentrations (100 μg/mL, 75 μg/mL, and 50 μg/mL) by disc diffusion method. The results showed the good antibacterial activity of FR-ZnONPs on G+ve and G-ve bacteria.

scholarly journals BIOGENIC SYNTHESIS OF SILVER NANOPARTICLES USING MANILKARA HEXANDRA (ROXB.) DUBARD STEM BARK EXTRACT AND IT’S PHYSICAL, CHEMICAL CHARACTERIZATION AND PHARMACEUTICAL EVALUATION

2019 ◽  
pp. 79-88
Author(s):  
A ANTONY LAWRENCE ◽  
J THOMAS JOSEPH PRAKASH
Keyword(s):  
Silver Nanoparticles ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Fourier Transform Infrared ◽  
Stem Bark ◽  
Diffusion Method ◽  
Bark Extract ◽  
Stem Bark Extract ◽  
Uv Visible

Objective: The present study was to synthesize nanoparticles using Manilkara hexandra stem bark extract its characterization and evaluating it by an antimicrobial and antioxidant assay. Methods: Manilkara hexandra stem bark silver nanoparticles (MHSB-AgNPs) was done by mixing silver nitrate (1 mmol) and aqueous stem bark extract and it was analyzed by UV-Visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), Zeta potential, Field Emission Scanning Electron Microscopy (FE-SEM), Energy Dispersive Spectroscopy (EDAX), Thermogravimetry/Differential Thermal Analysis (TG/DTA) and Differential scanning calorimetry (DSC). The antibacterial assay was done by a well diffusion method and also examined for antifungal assay was done by disk diffusion method and antioxidant potential Diphenyl-1-picryl hydrazyl (DPPH method) Results: Manilkara hexandra stem bark silver nanoparticles (MHSB-AgNPs) is characterized by various techniques such as UV-visible absorption spectrum ranges from 430 nm to 440 nm indicate silver nanoparticles. The Fourier Transform Infrared Spectroscopy consists of biomolecules acts as capping agent to form silver nanoparticles. Field Emission Scanning Electron Microscopy shows particle size ranges from 15 nm to 50 nm. Energy Dispersive Spectroscopy shows the presence of Silver. X-ray Diffraction corresponds to face-centered lattice planes (111), (200), (220) and (311). Dynamic Light Scattering show the range of 68 nm and Zeta potential show the negative value of-17 nm which has high stability. Silver nanoparticles is also examined by Thermogravimetry/Differential Thermal Analysis (TG/DTA) and Differential scanning calorimetry (DSC) this project the thermal stability of the nanoparticles. The aqueous stem bark is also examined by UV-visible absorption spectrum, Fourier Transform Infrared Spectroscopy (FTIR), and Gas Chromatography-Mass Spectrometry (GCMS). In GCMS 20 compounds were identified. Silver nanoparticles show high zone of inhibition in antimicrobial assays and act as a good antioxidant agent. Conclusion: It is eco-friendly, non-toxic, and it’s easy to synthesis and it shows good result in an antimicrobial and antioxidant assay can be applied in a pharmaceutical application.

scholarly journals 3D Series Metal Complexes Containing Schiff Base Ligand with 2,2'-Bipyridine: Synthesis, Characterization and Assessment of Antifungal Activity

2021 ◽  
pp. 34-45
Author(s):  
B. Kpomah ◽  
U. Ugbune ◽  
T. E. Idu
Keyword(s):  
Antifungal Activity ◽  
Metal Ion ◽  
Synergistic Effects ◽  
Research Work ◽  
Pathogenic Fungi ◽  
Multidrug Resistant ◽  
Ftir Spectra ◽  
Diffusion Method ◽  
Penicillium Species ◽  
Chloride Complexes

The rapid increase in the number of multidrug-resistant of most pathogenic organisms is fast becoming a global concern, thus, the discovery of novel active pharmacological compounds against new targets is a matter of urgency. The incorporation of metal ions into organic ligands has introduced metal-organic drugs framework with synergistic effects for novel applications in the biological system. In this research work, metal(II) chloride complexes of copper, nickel and zinc containing methylphenylketone thiosemicarbazone (MPK-TSC) with 2,2’-bipyridine (bipy) were synthesized; they were further characterized by satisfactory microelemental analysis, Fourier Transform InfraRed (FTIR) spectra as well as electronic spectra study. The complexes are proposed to have the formulae [L1ML2(Cl2)] where M=metal ion, L1=methylphenylketone thiosemicarbazone   L2=2,2’-bipyridine. The complexes are of 1:2 (metal:ligand) stoichiometry and non-electrolytes in solution, the bidentate nature of the two ligands was evident from the FTIR spectra. The compounds were screened for their antifungal activity against four pathogenic fungi: Aspergillus niger, Penicillium Species, Rizopus and Candida albicans using disc diffusion method. The activities of the complexes have been found to be greater than those of the metal salts and the uncoordinated ligands.

Sign in / Sign up

Export Citation Format

Share Document