Biosynthesis and Characterisation of Silver Nanoparticles using Leaf Extract of Achras sapota l. for its Antimicrobial Activity

Nanotechnology is a field that is rapidly growing, making an impact in all spheres of human life. In the current study, silver nanoparticles were synthesized using the ethanolic leaf extract of Achras sapota. Characterization was carried out using UV-Visible spectroscopy, FTIR, XRD, SEM and TEM. The formation of AgNPs was confirmed through UV-Visible spectroscopy by the colour change. Based on the XRD pattern, the crystalline property of the AgNPs was established. The functional group present in the ethanolic leaf extract of Achras sapota is responsible for the reduction of the Ag+ ion which was studied through FTIR. From the SEM and TEM analysis, it was found that the formed nanoparticles are spherical in shape and nano in size. The biosynthesized AgNPs was evaluated for its antimicrobial activity against gram positive bacteria (S. aureus and L. bacillus) and gram negative bacteria (E. coli and P. aeruginosa) using disc diffusion for preliminary screening of antimicrobial activity and dilution method for evaluation of antibacterial effectiveness and effect of silver nanoparticles on bacterial growth and it was found to exhibit potential antimicrobial activity. The biosynthesized AgNPs was found to be efficient in terms of reaction time as well as stability, eco-friendly and cost effective.

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Bio Synthesis and Characterization of Silver Nanoparticles Using Lagenaria siceraria Leaf Extract and their Antibacterial Activity

International Letters of Chemistry Physics and Astronomy ◽

10.18052/www.scipress.com/ilcpa.38.35 ◽

2014 ◽

Vol 38 ◽

pp. 35-45 ◽

Cited By ~ 2

Author(s):

B. Anandh ◽

A. Muthuvel ◽

M. Emayavaramban

Keyword(s):

Silver Nanoparticles ◽

Leaf Extract ◽

Face Centered Cubic ◽

Lagenaria Siceraria ◽

Human Pathogens ◽

X Ray Diffraction ◽

Visible Spectroscopy ◽

X Ray ◽

Ft Ir ◽

Uv Visible

The present investigation demonstrates the formation of silver nanoparticles by the reduction of the aqueous silver metal ions during exposure to the Lagenaria siceraria leaf extract. The synthesized AgNPs have characterized by UV-visible spectroscopy, X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR) techniques. AgNPs formation has screened by UV-visible spectroscopy through colour conversion due to surface plasma resonance band at 427 nm. X-ray diffraction (XRD) confirmed that the resulting AgNPs are highly crystalline and the structure is face centered cubic (fcc). FT-IR spectrum indicates the presence of different functional groups present in the biomolecules capping the nanoparticles. Further, inhibitory activity of AgNPs and leaf extract were tested against human pathogens like gram-pastive (Staphylococcus aureus, Bacillus subtilis), gram-negative (Escherichia coli and Pseudomonas aeruginosa). The results indicated that the AgNPs showed moderate inhibitory actions against human pathogens than Lagenaria siceraria leaf extract, demonstrating its antimicrobial value against pathogenic diseases

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Biosynthesis of silver nanoparticles using lavender leaf and their applications for catalytic, sensing, and antioxidant activities

Nanotechnology Reviews ◽

10.1515/ntrev-2016-0041 ◽

2016 ◽

Vol 5 (6) ◽

Cited By ~ 11

Author(s):

Brajesh Kumar ◽

Kumari Smita ◽

Luis Cumbal

Keyword(s):

Silver Nanoparticles ◽

Leaf Extract ◽

Antioxidant Activities ◽

Present Report ◽

X Ray Diffraction ◽

Visible Spectroscopy ◽

X Ray ◽

Transmission Electron ◽

Red Color ◽

Uv Visible

AbstractThe present report summarizes an eco-friendly approach for the biosynthesis of silver nanoparticles (AgNPs) using the leaf extract of lavender. Initially, the synthesis of AgNPs was visually observed by the appearance of a wine red color. The optical property, morphology, and structure of as-synthesized AgNPs were characterized by UV-visible spectroscopy, dynamic light scattering, transmission electron microscopy, and X-ray diffraction analyses. All characterization data revealed the formation of crystalline and spherical AgNPs (Ag/Ag

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Green Synthesis of Silver Nanoparticle Using Banana (Musa) Sap

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1086.7 ◽

2015 ◽

Vol 1086 ◽

pp. 7-10 ◽

Cited By ~ 3

Author(s):

S. Deena ◽

Arun Dakshinamurthy ◽

Paulraj Mosae Selvakumar

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Green Synthesis ◽

Silver Nanoparticle ◽

Gram Negative Bacteria ◽

Visible Spectroscopy ◽

High Antimicrobial Activity ◽

Gram Positive Bacteria ◽

Coated Fabric ◽

Uv Visible

Green synthesis of silver nanoparticles using banana sap as a solvent, reducing agent and capping agent is reported in this work. Banana sap has also been used as a binder to incorporate silver nanoparticles into cotton fabric. UV-Visible spectroscopy was used to monitor the quantitative formation of silver nanoparticles. Silver nanoparticle coated fabric showed high antimicrobial activity against gram negative bacteria such asPseudomonas aureginosaandEscherichia coli, and gram positive bacteria such asBacillus subtilisandStaphylococcus aureus.KeywordsGreen synthesis, Silver nanoparticles, banana sap, antimicrobial activity

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Green Synthesis of Silver Nanoparticles using Ajuga parviflora Benth and Digera muricata Leaf extract: Their Characterization and Antimicrobial Activity

Revista de Chimie ◽

10.37358/rc.20.10.8349 ◽

2020 ◽

Vol 71 (10) ◽

pp. 50-57

Author(s):

Kamran Mehdi ◽

Wajid Rehman ◽

Obaid-Ur-rahman Abid ◽

Srosh Fazil ◽

Muhammad Sajid ◽

...

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Leaf Extract ◽

State Of The Art ◽

Sem Analysis ◽

Bacterial Strains ◽

Transmission Electron ◽

Protein Amino Acids ◽

Uv Visible ◽

Average Size

The aim of the present study is to search out nontoxic silver nanoparticles synthesized from the leaf extract of two plants Ajuga parviflora Benth and Digera muricata for antimicrobial activity. The plants used in this investigation are rich in alkaloids, flavonoids, steroids, terpenoids, protein, amino acids, carbohydrate, quninones, phenols and tannins. The formation of nanoparticles were confirmed by UV/Visible spectroscopy, peaks at 423nm for Ajuga parviflora Benth and 408nm for Digera muricata. The morphology of the silver nanoparticles was established through state of the art spectroscopic tools. SEM analysis reveals average size of AgNPs 18 nm for Digera muricata and 22 nm for Ajuga parviflora Benth respectively while transmission electron microscopy confirms that AgNPs are spherical in shape. The synthesized nanoparticles were subjected to Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and Pseudomonas aeruginosa. The results suggest that the silver nanoparticles have promising activity against all the bacterial strains and can be used an effective bactericides.

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A Comparative Study for Detection of Melamine in Solution by Interference Sensing Using Functionalized Silver Nanoparticles

Advanced Science Letters ◽

10.1166/asl.2018.12208 ◽

2018 ◽

Vol 24 (8) ◽

pp. 5849-5854

Author(s):

Shrey A Shah ◽

Rahul Gundesha ◽

Jayesh P Ruparelia

Keyword(s):

Silver Nanoparticles ◽

Comparative Study ◽

Rapid Detection ◽

Leaf Extract ◽

Room Temperature ◽

Calotropis Procera ◽

Visible Spectroscopy ◽

Leaf Extracts ◽

Functionalized Nanoparticles ◽

Uv Visible

In this research an attempt has been made for instantaneous sensing of melamine, a potential milk adulterant, at room temperature with the help of bio-functionalized silver nanoparticles. The bio-functionalized nanoparticles were made using leaf extracts of Parthenium hysterophorous and Calotropis procera. Also these methods were compared with the nanoparticles that were functionalized with sulfanillic acid. Colorimetric change was the basis of sensing the melamine using these bio-functionalized silver nanoparticles. Melamine upto a limit of 0.1 ppm was detected by the particles prepared using Parthenium hysterophorous leaf extract at room temperature. UV-visible spectroscopy (200–800 nm range) was used to detect the changes in the absorbance of silver nanoparticles with the addition of melamine in different concentrations. DLS studies were carried out to confirm the presence of nanoparticles and to study their surface morphology during sensing. Comparative study showed that Parthenium hysterophorous (noxious hysterophorous) leaf extract was far better and accurate in detecting melamine and was as accurate as the silver nanoparticles functionalized with sulfanillic acid. This can be attributed to the fact that Parthenium hysterophorous leaf extract contains caffeic acid which worked as a reducing agent and also as the bio functionalized chemical. Thus, this method can be further used as a rapid detection of melamine. In addition, this method can be converted into prototype for detection of melamine in industries.

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Effect of Different Process Parameters on the formation of Silver Nanoparticles using Crude and Modified Neem (Azadirachta indica) Leaf Extracts

Advances in Technology ◽

10.31357/ait.v1i2.4855 ◽

2021 ◽

Vol 1 (2) ◽

Author(s):

Kalaivani Vivehananthan ◽

◽

W.H. De Silva

Keyword(s):

Silver Nanoparticles ◽

Process Parameters ◽

Leaf Extract ◽

Visible Range ◽

Mixing Ratio ◽

Visible Spectroscopy ◽

Leaf Extracts ◽

Maximum Absorbance ◽

Uv Visible ◽

Neem Leaf Extract

In recent times, the biosynthesis of nanoparticles, which has led to significant growth in the field of nanotechnology. The use of plant extracts has become an impetus in this field as it is a simple and eco-friendly method. This study was an attempt to study different parameters in biosynthesis of silver nanoparticles using Azadirachta indica (Neem) leaf extracts. Four different process parameters such as concentrations of neem leaf extract, types of neem leaf extract, mixing ratios and the reaction time period were investigated on the formation of silver nanoparticles. Initially, the formation of silver nanoparticles was detected by the visual observation. Then, the synthesized silver nanoparticles were characterized using UV-Visible spectroscopy and scanning electron microscopy (SEM). The change of color from yellow to reddish brown color confirmed the formation of silver nanoparticles. The silver surface plasmon resonance (SPR) band obtained in the expected visible range of UV-Visible spectroscopy confirmed the synthesis of the nanoparticles. SEM images showed that silver nanoparticles are roughly spherical and of uniform particle size, and the average particle size is 100 nm. Further, the maximum absorbance of SPR band was considerably varied with different process parameters used in the present study. The UV-Visible spectra of 2.5 g/100 mL of crude neem leaf extract without any dilution showed maximum absorbance in the expected range with the mixing ratio of (Neem and AgNO3) 1:8. However, the maximum absorbance of modified neem leaf extracts (pH 10) resulted lower in value than the crude extracts in the 20 times diluted sample with the mixing ratio of 1:9. Moreover, modified extract with UV radiation exposure increased the absorbance in the expected visible range. It concludes that fine tuning of the bioprocess parameters would enhance nanoparticle synthesis.

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Green Synthesis of Silver Nanoparticles by Allamanda cathartica L. Leaf Extract and Evaluation for Antimicrobial Activity

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2013.6.4.9 ◽

2013 ◽

Vol 6 (4) ◽

pp. 2260-2268

Author(s):

M. Linga Rao ◽

Bhumi G ◽

Savithramma N

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Leaf Extract ◽

Fusarium Species ◽

Inhibition Zone ◽

Fungal Species ◽

Green Technology ◽

Metallic Silver ◽

Synthesis Of Nanoparticles ◽

Aqueous Leaf Extract

Silver nanoparticles (SNPs) exhibit tremendous applications in medicine as antimicrobial agent. The use of different parts of plants for the synthesis of nanoparticles is considered as a green technology as it does not involve any harmful chemicals. In the present study, we report a rapid biosynthesis of silver nanoparticles from aqueous leaf extract of medicinal plant Allamanda cathartica. The active phytochemicals present in the plant were responsible for the quick reduction of silver ion to metallic silver nanoparticles. The reduced silver nanoparticles were characterized by using UV-Vis spectrophotometry, Scanning Electron Microscope (SEM), Energy Dispersive Analysis of X-ray (EDAX) and Atomic Force Microscopy (AFM). The spherical shaped silver nanoparticles were observed and it was found to 19-40 nm range of size. These phytosynthesized SNPs were tested for their antimicrobial activity and it analyzed by measuring the inhibitory zone. A. cathartica aqueous leaf extract of SNPs showed highest toxicity to Pseudomonas followed by Klebsiella, Bacillus and E. coli and lowest toxicity towards Proteus. In fungal species, highest inhibition zone was noted against Rhizopus followed by Curvularia, Aspergillus flavus and Aspergillus niger and minimum inhibition zone was observed against Fusarium species. These results suggest a promising potential of Indian plant-based green chemistry for production of SNPs for biomedical and nanotechnology applications.

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Antimicrobial Activity of Silver Nanoparticles Synthesized by Streptomyces Species JF714876

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2012.5.1.7 ◽

2012 ◽

Vol 5 (1) ◽

pp. 1638-1642 ◽

Cited By ~ 2

Author(s):

Vidyasagar G M ◽

Shankaravva B ◽

R Begum ◽

Imrose ◽

Sagar R ◽

...

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Silver Ions ◽

Human Beings ◽

Streptomyces Species ◽

Force Microscopy ◽

E Coli ◽

Extracellular Synthesis ◽

Atomic Force ◽

Uv Visible

Microorganisms like fungi, actinomycetes and bacteria are considered nanofactories and are helpful in the production of nanoparticles useful in the welfare of human beings. In the present study, we investigated the production of silver nanoparticles from Streptomyces species JF714876. Extracellular synthesis of silver nanoparticles by Streptomyces species was carried out using two different media. Silver nanoparticles were examined using UV-visible, IR and atomic force microscopy. The size of silver nanoparticles was in the range of 80-100 nm. Antimicrobial activity of silver nanoparticle against bacteria such as E. coli, S. aureus, and dermatophytes like T. rubrum and T. tonsurans was determined. Thus, this study suggests that the Streptomyces sp. JF741876 can produce silver ions that can be used as an antimicrobial substance.

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Green synthesis of silk sericin-embedded silver nanoparticles and their antibacterial application against multidrug-resistant pathogens

Journal of Genetic Engineering and Biotechnology ◽

10.1186/s43141-021-00176-5 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Md. Abdullah Al Masud ◽

Hamid Shaikh ◽

Md. Shamsul Alam ◽

M. Minnatul Karim ◽

M. Abdul Momin ◽

...

Keyword(s):

Silver Nanoparticles ◽

Green Synthesis ◽

Bombyx Mori ◽

Multidrug Resistant ◽

Visible Spectroscopy ◽

Silk Sericin ◽

X Ray ◽

Synthesis Strategy ◽

Uv Visible ◽

Multidrug Resistant Pathogens

Abstract Background The green synthesis strategy of metallic nanoparticles (NPs) has become popular due to being environmentally friendly. Stable silver nanoparticles (AgNPs) have been synthesized by natural products such as starch, soy protein, various extract of leaves, barks, and roots functioning both as reducing and stabilizing agents. Likewise, silk sericin (SS) is a globular protein discarded in the silk factory might be used for NP synthesis. In this research, we focus on the green synthesis and stabilization of AgNPs by SS as well as assessment of their antibacterial activities against some drug-resistant pathogen. Results SS was extracted from Bombyx mori silkworm cocoons in an aqueous medium. 17 w/w% of dry sericin powder with respect to the cocoon’s weight was obtained by freeze-drying. Furthermore, AgNPs conjugated to sericin, i.e., SS-capped silver nanoparticles (SS-AgNPs) were synthesized by easy, cost-effective, and environment-friendly methods. The synthesized SS-AgNPs were characterized by UV-visible spectroscopy, Fourier-transform infrared-attenuated total reflection (FTIR-ATR) spectroscopy, transmission electron microscopy (TEM), and X-ray diffraction measurement. It has been found from the absorbance of UV-visible spectroscopy that a higher percent of SS-AgNPs was obtained at a higher concentration of silver nitrate solution. FTIR-ATR spectra showed that the carboxylate groups obtained from silk sericin act as a reducing agent for the synthesis of silver nanoparticles, while NH2+ and COO− act as a stabilizer of AgNPs. The X-ray diffractogram of SS-AgNPs was quite different from AgNO3 and sericin due to a change in the crystal structure. The diameter of AgNPs was around 20–70 nm observed using TEM. The synthesized SS-AgNPs exhibited strong antibacterial activity against multidrug-resistant pathogens, Escherichia coli and Pseudomonas aeruginosa. Minimal inhibitory/bactericidal concentrations against E. coli and P. aeruginosa were 20μg/mL. Conclusions This study encourages the use of Bombyx mori for the ecofriendly synthesis of SS-AgNPs to control multidrug-resistant microorganisms.

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