scholarly journals The influence of thiotriazoline on the change of immune system in the blood of guinea-pigs under the conditions of development of experimental bronchial asthma

2015 ◽  
Vol 17 (2) ◽  
Author(s):  
M. S. Reheda ◽  
М. А. Kolishetska ◽  
V. R. Yurevych
Keyword(s):  
Immune System ◽  
T Lymphocytes ◽  
Blood Plasma ◽  
B Lymphocytes ◽  
Bronchial Asthma ◽  
Functional State ◽  
Immune Complexes ◽  
Guinea Pigs ◽  
Stimulation Of ◽  
Plasma Activity

<p>The aim of our research was to determine the character of the role and functional state of separate indexes<br />of the immune system in blood of guinea-pigs under the conditions of the development of experimental bronchial<br />asthma (BA ) and estimation of thiotriazoline influence on them. Decreasing of T-lymphocytes, stimulation of humoral<br />link of immunity, namely increasing of B-lymphocytes and immunoglobulins of A, M and G, elevation of circulatory<br />immune complexes and slump of complement blood plasma activity had been determined in this research. Immune<br />correcting action of thiotriazoline upon the pointed out indices in case of BA is revealed.</p>

scholarly journals ОСОБЛИВОСТІ ІМУННОГО ГОМЕОСТАЗУ В ДИНАМІЦІ РОЗВИТКУ ЕКСПЕРИМЕНТАЛЬНОЇ БРОНХІАЛЬНОЇ АСТМИ

2018 ◽  
Author(s):  
M. S. Reheda ◽  
L. A. Lubinets ◽  
B. F. Shchepanskyi
Keyword(s):  
Immune System ◽  
B Lymphocytes ◽  
Immune Complexes ◽  
Guinea Pigs ◽  
Circulating Immune Complexes ◽  
System Response ◽  
Control Group ◽  
Modeling Process ◽  
Immune System Response

In this paper, it is shown that the modeling process of bronchial asthma (BA ) is accompanied by changes, in comparison with the control group, in the indicators of immune system response: T- & B-lymphocytes, circulating immune complexes in blood of guinea pig males on the 4th, 18th, 25th day of experiment.The aim of the study – determination of some indices of immune system in blood of guinea pigs in the modeling process of BA on 4th, 18th, 25th day of the experiment.Materials and Methods. Experiments were conducted on 40 guinea pigs (males), with body weight 0.25–0.27 kg. Animals were divided into four groups of ten animals in each. Intact guinea pigs were the first group. Animals with experimental BA – the second, third, fourth group respectively on the 4th, 18th, 25th day of the experiment. Experimental BA was reproduced by V. I. Babych method. In blood of intact guinea pigs and animals with experimental BA , the number of T- and B-lymphocytes was determined by the method of E. F. Chernushenko, L. S. Kohosov, determination of the level of circulating immune complexes was carried out by the method of V. Haskova and co-authors. The results of the study were processed by the method of variation statistics using Student's criterion.Results and Discussion. The results of the studies showed unidirectional changes in individual parameters of the immune system, depending on the periods of the formation of BA : an increase in the number of B-lymphocytes and circulating immune complexes, a decrease in the number of T-lymphocytes for all of the studied days of the experiment.Conclusions. The obtained results indicate significant changes in the immune system parameters in the blood of experimental animals with BA and are important for understanding the pathogenesis of BA . These studies provide an opportunity to find the more perfect and effective methods of diagnosis of BA.

scholarly journals Порушення імунного гомеостазу в ранній період розвитку виразкової хвороби шунка на тлі експериментальної пневмонії

2017 ◽  
Author(s):  
L. O. Furdychko
Keyword(s):  
Gastric Ulcer ◽  
Immune System ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Immune Complexes ◽  
Experimental Studies ◽  
Early Period ◽  
Circulating Immune Complexes ◽  
Immune Homeostasis ◽  
Experimental Pneumonia

Introduction. The article adduces the obtained results of the indices of immune homeostasis in the development of combined pathology (experimental pneumonia (EP) and gastric ulcer (GU) of the stomach). The immune system is characterized by the number of T-lymphocytes since they are the basis of the cellular component of the immuneresponse and the concentration of b-lymphocytes and circulating immune complexes (CIC), which are responsible for cellular immunity.The aim of the study – the content of T-lymphocytes, CEC to assess the immune system in the development of EP and GU.Materials and Methods. This experimental study was conducted on 39 guinea pigs-males. Experimental pneumonia caused by the method of V. N. Shliapnikova, T. L. Solodova [et al.] Gastric ulcer of the stomach wassimulated by the method of V. I. Komarova. We determined the content of T- and B-lymphocytes (SD3 and SD19) in the blood by the method of E. F. Chernushenko, L. S. Kohosova. The level of circulating immune complexes (CIC) in the blood by the method of Haskova V., Kaslik J..Results and Discussion. The results of experimental studies established that early period of development (4th and 8th day) of gastric ulcer and pneumonia is characterized by the following changes of immune homeostasis: decrease in the level of T-lymphocytes and elevated levels of lymphocytes and circulating immune complexes in the blood of Guinea pigs.Conclusion. A depression of cell growth and humoral types of immunity in terms of experimental pneumoniaand gastric ulcer was revealed.  

scholarly journals T lymphocyte-dependent B lymphocyte proliferative response to antigen. I Genetic restriction of the stimulation of B lymphocyte proliferation.

1981 ◽  
Vol 153 (4) ◽  
pp. 871-882 ◽  
Author(s):  
H Y Tse ◽  
J J Mond ◽  
W E Paul
Keyword(s):  
T Lymphocytes ◽  
B Lymphocytes ◽  
T Lymphocyte ◽  
Lymphocyte Proliferation ◽  
B Lymphocyte ◽  
Antigen Specificity ◽  
Apparent Lack ◽  
The Face ◽  
Stimulation Of

For the purpose of examining more closely the interaction between T and B lymphocytes, we have developed an in vitro T lymphocyte-dependent B lymphocyte proliferation assay. Proliferation of B lymphocytes in response to antigen was found to depend on the presence of primed T lymphocytes; the B lymphocytes could be derived from nonprimed animals. It appears that these B cells were nonspecifically recruited to proliferate. This nonspecific recruitment, however, was found to be Ir-gene restricted in that B lymphocytes from B10.S mice, which are genetic nonresponders to the polymer Glu60-Ala30-Tyr10 (GAT), could not be stimulated by GAT-primed (responder X nonresponder) F1 T cells. The apparent lack of antigen specificity in the face of Ir gene-restricted T-B interaction may have important implications in our understanding of the recognition unit(s) on T lymphocytes.

scholarly journals A reasonable Approach for the Treatment of HIV Infection in the Early Phase with Ozonetherapy (Autohaemotherapy). How ‘Inflammatory’ Cytokines may have A therapeutic Role

1994 ◽  
Vol 3 (5) ◽  
pp. 315-321 ◽  
Author(s):  
V. Bocci
Keyword(s):  
Immune System ◽  
T Lymphocytes ◽  
Hiv Infection ◽  
Inflammatory Cytokines ◽  
Early Phase ◽  
Mechanisms Of Action ◽  
Therapeutic Role ◽  
Immunoregulatory Cytokines ◽  
Reasonable Approach ◽  
Stimulation Of

Immunoregulatory cytokines produced by the TH1 subset and by CD8+T lymphocytes appear to brake naturally and sometimes arrest the progress of HIV infection in the early phase. It appears reasonable to assume that a mild and equilibrated stimulation of the immune system may prevent or delay the fatal transition towards the prevalent production of TH2-type cytokines. The problem is how to stimulate the immune system in a physiological fashion. In the last 7 years we have clarified the main mechanisms of action of an unorthodox immunotherapeutic method first used 40 years ago. Optimized autohaemotherapy after a brief exposure ofblood to ozone may today afford the trick of reprogramming the immune system to keep HIV at bay. The autohaemotherapeutic procedure is simple, safe, inexpensive and most likely is more effective than conventional approaches.

scholarly journals The effect of interferons-α and –γ and their combination with dimethyl dipyrazolyl selenide on the immune status and functional state of the reproductive system of cows in adverse ecological conditions

2019 ◽  
pp. 53-57
Author(s):  
A. G. Shakhov ◽  
I. T. Shaposhnikov ◽  
Yu. N. Brigadirov ◽  
V. N. Kotsarev ◽  
V. N. Skorikov ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Functional State ◽  
Reproductive System ◽  
Immune Complexes ◽  
Immune Status ◽  
Lysozyme Activity ◽  
Circulating Immune Complexes ◽  
Control Group ◽  
Ecological Conditions ◽  
Positive Effect

The results of studying the effect of interferons alpha and gamma and their combination with dimethyl dipyrazolyl selenide on the immune status and functional state of the reproductive system of cows in adverse ecological conditions are presented. The positive effect of the drugs, especially their combination, on erythropoiesis and the immune status of animals, which was manifested by an increase in the content of erythrocytes, hemoglobin, hematocrit, leukocytes, total immunoglobulins, serum bactericidal and lysozyme activity, absorptive function of phagocytes, relative amount of T lymphocytes, and a decrease in the level of circulating immune complexes was stated. An increase in the immune status of animals at the effect of immunocorrecting drugs was accompanied by a decrease in the pathology of calving and postpartum complications and favorably affected the functional state of their reproductive system. The cows which were introduced interferons and their combination with dimethyl dipyrazolyl selenide demonstrated a shorter period from calving to successful insemination, as well as the insemination index and fertility, compared with the animals of the control group, which were not introduced any drugs.

scholarly journals Inducible Polarized Secretion of Exosomes in T and B Lymphocytes

2020 ◽  
Vol 21 (7) ◽  
pp. 2631 ◽  
Author(s):  
Victor Calvo ◽  
Manuel Izquierdo
Keyword(s):  
Immune System ◽  
T Lymphocytes ◽  
Cell Surface ◽  
B Lymphocytes ◽  
Synaptic Cleft ◽  
T And B Lymphocytes ◽  
Cell Surface Receptors ◽  
Microtubule Organization ◽  
Cell Receptors ◽  
Surface Receptors

Exosomes are extracellular vesicles (EV) of endosomal origin (multivesicular bodies, MVB) constitutively released by many different eukaryotic cells by fusion of MVB to the plasma membrane. However, inducible exosome secretion controlled by cell surface receptors is restricted to very few cell types and a limited number of cell surface receptors. Among these, exosome secretion is induced in T lymphocytes and B lymphocytes when stimulated at the immune synapse (IS) via T-cell receptors (TCR) and B-cell receptors (BCR), respectively. IS formation by T and B lymphocytes constitutes a crucial event involved in antigen-specific, cellular, and humoral immune responses. Upon IS formation by T and B lymphocytes with antigen-presenting cells (APC), the convergence of MVB towards the microtubule organization center (MTOC), and MTOC polarization to the IS, are involved in polarized exosome secretion at the synaptic cleft. This specialized mechanism provides the immune system with a finely-tuned strategy to increase the specificity and efficiency of crucial secretory effector functions of B and T lymphocytes. As inducible exosome secretion by antigen-receptors is a critical and unique feature of the immune system this review considers the study of the traffic events leading to polarized exosome secretion at the IS and some of their biological consequences.

scholarly journals Stimulation of HIV-1 Replication in Immature Dendritic Cells in Contact with Primary CD4 T or B Lymphocytes

2010 ◽  
Vol 84 (9) ◽  
pp. 4172-4182 ◽  
Author(s):  
Vincent Holl ◽  
Ke Xu ◽  
Maryse Peressin ◽  
Alexandre Lederle ◽  
Marina Elizabeth Biedma ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Neutralizing Antibodies ◽  
Mononuclear Cells ◽  
Cell Contact ◽  
Specific Marker ◽  
Experimental Conditions ◽  
Hiv 1 ◽  
Stimulation Of

ABSTRACT Sexual transmission is the major route of HIV-1 infection worldwide. Dendritic cells (DCs) from the mucosal layers are considered to be the initial targets of HIV-1 and probably play a crucial role in HIV-1 transmission. We investigated the role of cell-to-cell contact between HIV-1-exposed immature DCs and various lymphocyte subsets in the stimulation of HIV-1 replication. We found that HIV-1 replication and production in DCs were substantially enhanced by the coculture of DCs with primary CD4 T or nonpermissive B lymphocytes but not with primary activated CD8 T lymphocytes or human transformed CD4 T lymphocytes. Most of the new virions released by cocultures of HIV-1-exposed immature DCs and primary B lymphocytes expressed the DC-specific marker CD1a and were infectious for both immature DCs and peripheral blood mononuclear cells (PBMCs). Cocultured DCs thus produced large numbers of infectious viral particles under these experimental conditions. The soluble factors present in the supernatants of the cocultures were not sufficient to enhance HIV-1 replication in DCs, for which cell-to-cell contact was required. The neutralizing monoclonal antibody IgG1b12 and polyclonal anti-HIV-1 sera efficiently blocked HIV-1 transfer to CD4 T lymphocytes but did not prevent the increase in viral replication in DCs. Neutralizing antibodies thus proved to be more efficient at blocking HIV-1 transfer than previously thought. Our findings show that HIV-1 exploits DC-lymphocyte cross talk to upregulate replication within the DC reservoir. We provide evidence for a novel mechanism that may facilitate HIV-1 replication and transmission. This mechanism may favor HIV-1 pathogenesis, immune evasion, and persistence.

scholarly journals PECULIARITIES OF POST-FIRM REHABILITATION PIGLES DEPENDING ON THE SEASON OF BIRTH

2021 ◽  
pp. 122-128
Author(s):  
N. V. Efanova ◽  
L. M. Osina ◽  
S. V. Batalova
Keyword(s):  
Immune System ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Immune Status ◽  
Season Of Birth ◽  
B Lymphopoiesis ◽  
T Helper ◽  
Low Level ◽  
Different Seasons ◽  
Activated T Lymphocytes

The features of the rehabilitation of the immune system of piglets after weaning were studied in connection with different seasons of farrowing. To fulfill this goal, 4 groups were formed from the offspring of sows of the SM-1 breed of Novosibirsk selection. The 1st group included piglets from winter farrowings, and the 2nd, 3rd and 4th groups, respectively, young animals born in spring, summer and autumn. Each group consisted of 30 animals. Studies of indicators of the immune status were carried out before weaning and 30 days after weaning. Weaning of piglets from sows with their subsequent regrouping in the educational farm «Tulinskoye» is carried out at the age of 60 days. The research results have shown that the state of the immune status of 2- and 3-month-old pigs is seasonal. Piglets born in autumn show the best indicators of leukopoiesis, T-, B-lymphopoiesis, the formation of functionally mature T-lymphocytes, activated T-lymphocytes and antitelogenesis. The second position in terms of the intensity of leukopoiesis, antitelogenesis and B-lymphopoiesis is occupied by young animals born in summer. Piglets raised in winter differ from their peers in a low level of IgM synthesis, while piglets born in spring have the lowest total numbers of T-lymphocytes, T-helper inductors, T-killer suppressors, B-lymphocytes and IgG. A month after weaning, animals, regardless of the season of birth, showed a statistically significant decrease in T-lymphopoiesis. The activity of the T-link of the immune system decreased most significantly in young animals obtained in spring. However, in contrast to their peers, youngsters born in the spring showed a statistically significant increase in blood IgM and IgG concentrations. In piglets born in winter, summer and autumn, the activity of IgM synthesis, on the contrary, decreased, while the formation of IgG remained at the same level.

scholarly journals The condition of piglet immune system during post-weaning period depending on the sex and stress-reactivity

2021 ◽  
pp. 98-105
Author(s):  
N. V. Efanova ◽  
V. V. Gart ◽  
К. V. Zhuchaev ◽  
L. M. Osina ◽  
S. V. Batalova
Keyword(s):  
T Cells ◽  
Immune System ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Stress Reactivity ◽  
Helper T Cells ◽  
T And B Lymphocytes ◽  
Antibody Synthesis ◽  
Igg Synthesis ◽  
High Proliferative Activity

The immune system of 90-day old piglets of SM-1 Novosibirsk breed piglets depends on sex and stress-reactivity. Stress-reactivity was measured using halothane test. The immunologic testing was performed 30 days after weaning. Our results show that overall piglet immune system demonstrated high proliferative activity of T- and B- immunocompetent cells with active formation of mature active T-and B-lymphocytes and did not show signs of immunosuppression. Compared to guilts, barrows had higher concentration of leucocytes, T-and B-lymphocytes, killer-supressor T-cells, activated and poorly differentiated T-lymphocytes. Gilts had higher production of inductor-helper T-cells, IgM and IgG when compared to barrows. Stress-resistant piglets had higher concentration of B-lymphocytes, IgM and IgG whereas stress-sensitive piglets had higher concentration of T-lymphocytes, supressor-killer T-cells and thymus T-lymphocytes. Gilts had higher concentration of inductor-helper T-cells than killer-supressor T-cells. Gilts overall had intensive antibody synthesis, however, stress-resistant gilts had higher IgG synthesis compared to stress-sensitive gilts. In barrows immature T-lymphocytes differentiated mainly into killer-supressor T-cells. The adaptivity of barrow immune system was characterized by high circulatory B-lymphocytes and IgM. Stress-sensitive barrows had lower antibody synthesis levels and higher T-lymphophoesis compared to stress-resistant barrows. 

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