Morphological and molecular identification of specimens in the genus Euseius (Acari: Phytoseiidae) from the Republic of Congo

Zootaxa ◽  
2020 ◽  
Vol 4768 (4) ◽  
pp. 479-498
Author(s):  
MIREILLE BELLE MBOU OKASSA ◽  
DOLLON MBAMA NTABI ◽  
ARSENE LENGA
Keyword(s):  
Molecular Analysis ◽  
Dna Sequences ◽  
Principal Component ◽  
Taxonomic Status ◽  
Original Description ◽  
Integrative Taxonomy ◽  
Integrative Approach ◽  
12S Rrna ◽  
Dna Fragments ◽  
The Republic

The purpose of this study was to identify the taxonomic status of several individuals belonging to the genus Euseius, collected from three host plants (cassava, okra, and chilli) in the Republic of the Congo, using morphometric and molecular analysis. For this, hierarchical ascending classification on principal component analysis was performed using morphological measurements from 21 and 22 individuals of E. fustis and Euseius sp., respectively, with the original description of Euseius neodossei considered. For molecular analysis, two DNA fragments were amplified and sequenced: the mitochondrial 12S rRNA and the nuclear ribosomal region ITSS. This integrative approach found that (1) morphological differences existed between the Euseius sp. and both E. neodossei and E. fustis, and (2) both mitochondrial DNA fragments showed a clear delineation between Euseius sp. and E. fustis. The results obtained highlight the value of using integrative taxonomy to improve the identification of species in under-sampled areas and the need to increase the number of DNA sequences deposited in GenBank database from this region. This is the first time that DNA sequences of mites from the Congo (Brazzaville) have been deposited in this database. 

scholarly journals A new species of the genus Euseius Wainstein (Acari: Phytoseiidae) from Republic of Congo 

2021 ◽  
Author(s):  
Mireille Belle Mbou Okassa ◽  
Valentin Dibangou ◽  
Grace Nianga ◽  
Dollon Mbama Ntabi ◽  
Arsène Lenga
Keyword(s):  
Dna Sequences ◽  
Host Plants ◽  
Morphological Analysis ◽  
Integrative Approach ◽  
12S Rrna ◽  
Republic Of The Congo ◽  
Clear Delineation ◽  
The Republic ◽  
Morphological Differences ◽  
A New Species

Several individuals belonging to the genus Euseius, have been collected from three host plants (cassava, okra, and chilli) in the Republic of the Congo. Previous molecular and morphological analysis (integrative approach) have shown that 1) morphological differences existed between the Euseius sp. and both E. neodossei and E. fustis, and 2) both mitochondrial DNA fragments considered (i.e., 12S rRNA and ITSS) showed a clear delineation between Euseius sp. and E. fustis. It was not possible to compare these species with E. neodossei (a morphologically close species) owing to the lack of DNA sequences in GenBank for this species. The purpose of this study was to describe a news species Euseius congolensis sp. nov from Republic of the Congo.

scholarly journals Designation of the neotype of Triatoma dimidiata (Latreille, 1811) (Hemiptera, Reduviidae, Triatominae), with full integrated redescription including mitogenome and nuclear ITS-2 sequences

ZooKeys ◽  
2021 ◽  
Vol 1076 ◽  
pp. 9-24
Author(s):  
Silvia Andrade Justi ◽  
Carolina Dale
Keyword(s):  
Natural History ◽  
Dna Sequences ◽  
Taxonomic Status ◽  
Original Description ◽  
Sensu Stricto ◽  
Cryptic Diversity ◽  
American Museum ◽  
Internal Transcribed Spacer Region ◽  
Nuclear Its ◽  
Second Internal Transcribed Spacer

The taxonomic status of Triatoma dimidiata (Latreille, 1811) is, by far, the most discussed within Triatominae. Molecular studies have recovered at least three independently evolving lineages in T. dimidiata across its range. The original description of T. dimidiata (as Reduvius dimidiatus) included few taxonomic characters, and no types were assigned. To define and describe the cryptic diversity within T. dimidiata sensu lato (s.l.), a neotype must be designated. For this purpose, all 199 specimens identified as T. dimidiata from the collections of the Smithsonian Institution – National Museum of Natural History and the American Museum of Natural History, ranging from Peru to Mexico, were studied. Only one specimen (from Tumbes, Peru) matched the combination of characters as listed in the original description, and it is herein formally designated as the neotype for T. dimidiata. The neotype is morphologically described and DNA sequences of its whole mitochondrial genome and the nuclear second internal transcribed spacer region (ITS2), commonly used in triatomine molecular systematics studies, are presented and compared to other publicly available sequences of T. dimidiata s.l. in GenBank. Our results suggest that T. dimidiata sensu stricto (s.s.) is somewhat rare and, therefore, unlikely to serve as a major vector of Chagas disease.

Species diversity of Hyalinobatrachium glassfrogs (Amphibia: Centrolenidae) from the Guiana Shield, with the description of two new species

Zootaxa ◽  
2011 ◽  
Vol 3132 (1) ◽  
pp. 1 ◽  
Author(s):  
SANTIAGO CASTROVIEJO-FISHER ◽  
CARLES VILÀ ◽  
JOSÉ AYARZAGÜENA ◽  
MICHEL BLANC ◽  
RAFFAEL ERNST
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
French Guiana ◽  
Taxonomic Status ◽  
Integrative Taxonomy ◽  
Guiana Shield ◽  
Basic Information ◽  
Two New Species ◽  
Taxonomic Uncertainty ◽  
New Distribution

Basic information about the taxonomy, biology and distribution of Hyalinobatrachium glassfrogs of the Guiana Shield (GS) is scarce, ambiguous, and in many cases even contradictory. In this review we aim to clarify the current taxonomic status of this group by means of phenotypic (morphology, morphometrics and bioacoustics) and molecular (mitochondrial DNA sequences) comparisons. Eight species have previously been recognized for the GS: H. crurifasciatum, H. eccentricum, H. fleischmanni (initially described as Hylella cappellei in the GS), H. iaspidiense (with the putative synonym H. nouraguense), H. ignioculus, H. mesai, H. mondolfii, and H. taylori. Our data support the resurrection of H . cappellei from its synonymy with H. fleischmanni. Hyalinobatrachium crurifasciatum, H. eccentricum, and H. ignioculus are proposed as junior synonyms of H. cappellei. We show that none of the four paratypes of H. taylori belong to this species and we assign two to H. cappellei and two to H. mondolfii. Additional specimens previously identified as H. taylori are reassigned to H. cappellei, and hence H. taylori is redefined. Hyalinobatrachium nouraguense is confirmed as a junior syn-onym of H. iaspidiense. We also describe two new species of Hyalinobatrachium from French Guiana: Hyalinobatrachium kawense sp. nov. and Hyalinobatrachium tricolor sp. nov. In addition, and in concordance with the new taxonomic rearrangements, we provide diagnostic characters for all species, known distributions and main sources of references for their biology. We also report new distribution records for H. iaspidiense and H. mondolfii, and describe the formerly unknown tadpole of the later. Consequently, we recognize seven species of Hyalinobatrachium for the Guiana Shield: H. cappellei, H. iaspidiense, H. kawense sp. nov., H. mesai, H. mondolfii, H. taylori, and H. tricolor sp. nov. We discuss the suitability of integrative taxonomy as an approach to identify taxonomic uncertainty and consider its significance for conservation purposes. We also address the implications of our results to understand phylogeographic patterns in this area.

scholarly journals Resolving the Taxonomic Status of Potential Biocontrol Agents Belonging to the Neglected Genus Lipolexis Förster (Hymenoptera, Braconidae, Aphidiinae) with Descriptions of Six New Species

Insects ◽  
2020 ◽  
Vol 11 (10) ◽  
pp. 667
Author(s):  
Korana Kocić ◽  
Andjeljko Petrović ◽  
Jelisaveta Čkrkić ◽  
Nickolas G. Kavallieratos ◽  
Ehsan Rakhshani ◽  
...  
Keyword(s):  
Biological Control ◽  
New Species ◽  
Molecular Analysis ◽  
Phylogenetic Relationships ◽  
Morphological Study ◽  
Species Complex ◽  
Taxonomic Status ◽  
Integrative Approach ◽  
Control Programs ◽  
Small Genus

Lipolexis is a small genus in the subfamily Aphidiinae represented by one species in Europe (Lipolexis gracilis Förster) and by four in Asia (Lipolexis wuyiensis Chen, L. oregmae Gahan, L. myzakkaiae Pramanik and Raychaudhuri and L. pseudoscutellaris Pramanik and Raychaudhuri). Although L. oregmae is employed in biological control programs against pest aphids, the last morphological study on the genus was completed over 50 years ago. This study employs an integrative approach (morphology and molecular analysis (COI barcode region)), to examine Lipolexis specimens that were sampled worldwide, including specimens from BOLD database. These results establish that two currently recognized species of Lipolexis (L. gracilis, L. oregmae) are actually a species complex and also reveal phylogenetic relationships within the genus. Six new species are described and a global key for the identification of Lipolexis species is provided.

Spread of the introduced species Laurencia caduciramulosa (Rhodomelaceae, Rhodophyta) to the northwest Atlantic: A morphological and molecular analysis

Phytotaxa ◽  
2014 ◽  
Vol 183 (2) ◽  
pp. 93 ◽  
Author(s):  
Ligia Collado-Vides ◽  
VALÉRIA CASSANO ◽  
JHOANA DÍAZ-LARREA ◽  
ALAN DURAN ◽  
AMANDA DA SILVA MEDEIROS ◽  
...  
Keyword(s):  
Introduced Species ◽  
Molecular Analysis ◽  
Canary Islands ◽  
Dna Sequences ◽  
Original Description ◽  
Marine Macroalgae ◽  
Morphological Identification ◽  
Biscayne Bay ◽  
Marine Invasions ◽  
A New Species

Introduction of species is a common problem in marine environments; marine macroalgae in particular have been reported to be introduced in all oceans, mainly through transport in ballast water, ship fouling and aquarium trade. The majority of the reported alien seaweeds belong to the Rhodophyta. Recently several species of the Laurencia complex have been reported, contributing to an increase of the number of red algae being successfully introduced. Since its description as a new species from Vietnam in 1997, Laurencia caduciramulosa has been spreading steadily with disjunct reports consistently finding specimens close to harbors or major ports. Biscayne Bay, Florida, home to one of the largest ports in USA, and surrounded by the Miami metropolis, is prone to receiving introduced species. This study reports the first introduction of L. caduciramulosa in Biscayne Bay, and its spread to the NW Atlantic. Morphological analysis demonstrated that the specimens collected in Crandon Park, Biscayne Bay, are similar to other specimens of the same species described for Vietnam (original description), Brazil, the Canary Islands and Cuba. Furthermore, molecular analysis using chloroplast-encoded rbcL DNA sequences corroborated the morphological identification. The phylogenetic results suggested that populations in Brazil, Canary Islands and Cuba are recent introductions, while the position of the Florida clade can be interpreted as a distinct and earlier introduction. Due to the level of expertise and knowledge of the Laurencia complex available, we suggest that in the future this group can be used as a model to conduct population genetics analysis of all described introductions in relation to native populations in order to describe patterns of expansion and provide an insight of marine invasions processes. 

scholarly journals Integrated taxonomy supports the identification of some species of Phytoseiidae (Acari: Mesostigmata) from Georgia

Acarologia ◽  
2021 ◽  
Vol 61 (4) ◽  
pp. 824-844
Author(s):  
Marie-Stephane Tixier ◽  
Philippe Auger ◽  
Alain Migeon ◽  
Martial Douin ◽  
Amandine Fossoud ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Abundant Species ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
12S Rrna ◽  
Amblyseius Andersoni ◽  
Integrated Taxonomy ◽  
Well Differentiated ◽  
Mtdna Markers ◽  
Eleven Species

The present study reports results of a survey carried out mostly on Citrus sp. and Rubus sp. in Georgia. Morphological and molecular (12S rRNA, COI and CytB mtDNA markers) data were analysed in a framework of integrative taxonomy. Eleven species were identified and among them seven are new for the Georgian fauna. Euseius stipulatus and Phytoseius finitimus were the most abundant species during this survey. We assume that Amblyseius eharai, only reported from eastern Asia, was most probably introduced. Neoseiulus californicus, retrieved from uncultivated vegetation, was almost certainly originating from commercial strains. DNA sequences comparisons disclosed phylogenetic closeness between Amblyseius andersoni and Transeius wainsteini, despite these species (i) being morphologically well differentiated and (ii) classified in different genera, thereby questioning the reliability of the genus Transeius. General morphological characters, including measurements, are provided for species for which diagnoses were doubtful.

Redescription and molecular analysis of Pallisentis (Pallisentis) nandai Sarkar, 1953 (Acanthocephala: Quadrigyridae) in India

2021 ◽  
Vol 95 ◽  
Author(s):  
O.M. Amin ◽  
R.A. Heckmann ◽  
A. Chaudhary ◽  
N.Y. Rubtsova ◽  
H.S. Singh
Keyword(s):  
Molecular Analysis ◽  
Dna Sequences ◽  
Indian Subcontinent ◽  
Original Description ◽  
Ganga River ◽  
Phylogenetic Position ◽  
Molecular Evidence ◽  
Line Drawings ◽  
Variable Level ◽  
First Time

Abstract Pallisentis (Pallisentis) nandai Sarkar, 1953 is a freshwater fish parasite restricted to the Indian subcontinent in the Ganga River and its tributaries. It was described from the leaffish, Nandus nandus (Hamilton) from the Ganga River delta at Calcutta. We recovered variant specimens from the same host species from the Ganga near its headwaters at Bijnor about 1500 km away. Our specimens were clearly identifiable as P. nandai but varied considerably from those in the original description, especially in the size of proboscis hooks, receptacle and lemnisci. The original description was incomplete (missing line drawings of female trunk and reproductive system, male trunk, complete proboscis, hooks and hook roots) and inaccurate (proboscis, hooks, receptacle wall), and some measurements were lumped together for both sexes. We provide a complete description and include new morphological information including the first description of para-receptacle structure in the genus Pallisentis Van Cleave, 1928, scanning electron microscopy and microscope images, molecular analysis, and energy dispersive X-ray analysis (EDXA) of hooks and spines of our specimens for the first time. Additional details of proboscis hook roots, trunk spines, micropores and micropore distribution are described. The unique metal composition of hooks (EDXA) demonstrated a considerably high but variable level of sulphur and negligible level of calcium in collar and trunk spines and hook tips, but a higher level of sulphur and calcium at the hook basal arch than at the hook tip and edge. A comparison with the EDXA pattern of another species of Pallisentis, P. İndica Mital & Lal, 1976, were considerably different. The phylogenetic position of P. nandai within Eoacanthocephala was generated to assess the molecular characterization based on 18S and ITS1-5.8S-ITS2 ribosomal DNA sequences. Maximum likelihood and Bayesian inference analyses placed P. nandai in a clade with other Pallisentis species under the family Quadrigyridae. This is the first report based on molecular evidence for P. nandai.

Phylogenetic position of two Macrobiotus species with a revisional note on Macrobiotus sottilei Pilato, Kiosya, Lisi & Sabella, 2012 (Tardigrada: Eutardigrada: Macrobiotidae)

Zootaxa ◽  
2021 ◽  
Vol 4933 (1) ◽  
pp. 113-135
Author(s):  
YEVGEN KIOSYA ◽  
JUSTYNA POGWIZD ◽  
YELYZAVETA MATSKO ◽  
MATTEO VECCHI ◽  
DANIEL STEC
Keyword(s):  
Dna Sequences ◽  
Original Description ◽  
Integrative Taxonomy ◽  
Morphological Data ◽  
Phylogenetic Position ◽  
28S Rrna ◽  
Mitochondrial Coi ◽  
The Family ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis

In a moss and lichen sample collected on the Polish coast, a new population of Macrobiotus sottilei was found. Given that the original description of M. sottilei was based solely on the morphology observed under light microscopy and measurements of a few individuals, we provide, by means of integrative taxonomy, a revisional note on this species. We present a comprehensive set of morphometric and morphological data from light and scanning electron microscopy analysis together with nucleotide sequences of three nuclear (18S rRNA, 28S rRNA, ITS-2) and one mitochondrial (COI) DNA fragments. We also provide the same set of DNA sequences for Macrobiotus glebkai from a population recently found in Eastern Ukraine and elucidate the phylogenetic position of these two taxa within the family Macrobiotidae. Moreover, the terminology of cuticular bars in macrobiotid legs, and pillars within the egg chorion ornamentation in the Macrobiotus hufelandi morphogroup, are also discussed. 

Taxonomic revision of Algero-Tunisian Pleurodeles (Caudata: Salamandridae) using molecular and morphological data. Revalidation of the taxon Pleurodeles nebulosus (Guichenot, 1850)

Zootaxa ◽  
2004 ◽  
Vol 488 (1) ◽  
pp. 1 ◽  
Author(s):  
SALVADOR CARRANZA ◽  
EDWARD WADE
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome B ◽  
Morphometric Analysis ◽  
Dna Sequences ◽  
Taxonomic Status ◽  
Taxonomic Revision ◽  
Morphological Data ◽  
Morphological Evidence ◽  
12S Rrna

The taxonomic status of Algero-Tunisian Pleurodeles was reanalysed in the light of new molecular and morphological evidence. Mitochondrial DNA sequences (396 bp of the cytochrome b and 369 of the 12S rRNA) and the results of the morphometric analysis, indicate that Algero-Tunisian P. poireti consists of two genetically and morphologically distinct forms. One restricted to the Edough Peninsula, and another one covering all the rest of its distribution in Algeria and Tunisia. The name P. poireti (Gervais, 1835) is restricted to the population of the Edough Peninsula, while P. nebulous (Guichenot, 1850) correctly applies to all other populations in the distribution. P. poireti originated approximately 4.2 Myr ago, probably as a result of the Edough Peninsula being a Pliocene fossil island, allowing both forms of Algero-Tunisian Pleurodeles to diverge both genetically and morphologically.

scholarly journals New Records of Dactylobiotus parthenogeneticus Bertolani, 1982 Provide Insight into Its Genetic Variability and Geographic Distribution

2020 ◽  
Vol 68 (2) ◽  
pp. 57-72 ◽  
Author(s):  
Justyna Pogwizd ◽  
Daniel Stec
Keyword(s):  
Dna Sequences ◽  
18S Rrna ◽  
Haplotype Diversity ◽  
Original Description ◽  
Integrative Taxonomy ◽  
Morphological Data ◽  
Morphological Identification ◽  
28S Rrna ◽  
Mitochondrial Coi ◽  
Insight Into

In sediment samples collected from three distinct European locations (United Kingdom, France, Poland), populations of Dactylobiotus parthenogeneticus were found. The original description of this species was based solely on the morphology observed with light microscopy and later supplemented by some additional SEM data of the buccal apparatus and DNA sequences of 18S rRNA and COI. Here we provide an updated description of the species by means of integrative taxonomy. The description comprises a comprehensive set of morphometric and morphological data from light and scanning microscopy as well as nucleotide sequences of three nuclear (18S rRNA, 28S rRNA, ITS-2) and one mitochondrial (COI) fragments. Our analysis of haplotype diversity confirmed our morphological identification and showed that D. parthenogeneticus is widely distributed in Europe.

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