Radiolabeled D-Penicillamine Magnetic Nanocarriers for Targeted Purposes

2016 ◽  
Vol 16 (4) ◽  
pp. 4174-4179 ◽  
Author(s):  
Seniha Yolcular Özyüncü ◽  
Serap Teksöz ◽  
Çiğdem İçhedef ◽  
E. İlker Medine ◽  
Çığır Biray Avcı ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Human Breast ◽  
X Ray Diffraction ◽  
Mcf7 Cells ◽  
New Agents ◽  
X Ray ◽  
Amino Silane ◽  
Magnetic Nanocarriers ◽  
New Occurrences

The aim of this study is to synthesize D-Penicillamine (D-PA) conjugated magnetic nanocarriers for targeted purposes. Magnetic nanoparticles were prepared by partial reduction method and surface modification was done with an amino silane coupling agent’s (structural properties), AEAPS, the particles were characterized by Scanning Electron Microscope (SEM), X-ray Diffraction (XRD). After that D-PA was linked with the magnetic nanoparticles (MNPs) and has been radiolabeled with [99mTc(CO)3]+ core. Quality controls of [99mTc(CO)3-MNP-D-PA] were established by Cd(Te) detector. The radiolabeling efficiency of magnetic nanoparticles ([99mTc(CO)3-MNP-D-PA]) was about 97.05% with good in vitro stability during the 24 hour period. As a parallel study, radiolabeled D-PA complex ([99mTc(CO)3-D-PA]) was prepared with a radiolabeling yield of 97.93%. At the end, biologic activities of binding complexes were investigated on MCF7 human breast cancer cells. Our results show that, radiolabeled magnetic nanoparticles with core [99mTc(CO)3]+ ([99mTc(CO)3-MNP-D-PA]) showed the highest uptake on MCF7 cells which were applied magnetic field in the wells. In that case, result of this study emphasizes that radiolabeled magnetic nanoparticles with core [99mTc(CO)3]+ would support new occurrences of new agents.

scholarly journals Development of magnetic nanoparticles Fe+32 X+21O4 (X= Fe, Co y Ni) coated by amino silane

2017 ◽  
Author(s):  
◽  
I. A. Flores-Urquizo
Keyword(s):  
Magnetic Nanoparticles ◽  
Crystal Size ◽  
Organic Molecules ◽  
Magnetic Core ◽  
Stoichiometric Ratio ◽  
Vibrational Modes ◽  
X Ray Diffraction ◽  
X Ray ◽  
Amino Silane ◽  
Diffraction Patterns

Magnetic nanoparticles are proposed as heat mediators in hyperthermia treatments. In this work, three core-shell materials of different composition and magnetic anisotropy were developed to determine their properties as crystal size, saturation magnetization and their coating with organic molecules. The magnetic core of these materials was made by means of the coprecipitation reaction, following the stoichiometric ratio X+2Fe2+3O4 where X is Fe, Co or Ni for each material. From the X-ray diffraction patterns the crystal size of each material was determined, these were 10.39 nm, 7.27 nm y 3.86 nm. In addition, magnetization was 55.84 emu/g, 36.56 emu/g y 16.21 emu/g for magnetite, cobalt ferrite and nickel respectively. Each material was coated with aminosilane and by FTIR the vibrational modes of the C-N, N-H, C-H and Si-O bonds involved in the coating were identified.

scholarly journals Investigations into the Biocompatibility of Nanohydroxyapatite Coated Magnetic Nanoparticles under Magnetic Situation

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Qing Li ◽  
Gang Zhou ◽  
Tong Wang ◽  
Yongzhao Hou ◽  
Xuliang Deng ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Magnetic Nanoparticle ◽  
Magnetic Particles ◽  
X Ray Diffraction ◽  
New Approach ◽  
X Ray ◽  
In Vitro Experiment ◽  
Transmission Electron ◽  
Mesenchyme Stem Cells

Regenerative medicine consisting of cells and materials offers a new approach for repairing and regenerating the organs and tissues. More and more researches focused on the magnetic nanobiomaterials due to its superior advantages to traditional materials. However, the toxicity of nanosized magnetic particles cannot be ignored, especially under the magnetic situation. This study aims to study the biocompatibility of nanohydroxyapatite (n-HA-) coated magnetic nanoparticles under the magnetic situation. n-HA-coated magnetic nanoparticles were fabricated through an ultrasound-assisted coprecipitation method. Subsequently, these materials were analyzed by transmission electron microscope (TEM) and X-ray diffraction (XRD) and then were cultured with mesenchyme stem cells derived from human bone marrow (hMSC-BM). In vitro experiment proved the satisfactory biocompatibility of n-HA-coated magnetic nanoparticles. These important factors (ALP, OCN, and OPN) influence the osteogenic differentiation of hMSC-BM. It was found that the hMSC-BM with combination of n-HA/Fe3O4and magnetic stimulation presented higher degree of osteoblast-related markers than that in each alone. This research demonstrated that a novel nanohydroxyapatite coated magnetic nanoparticle is safe under the magnetic situation. Therefore, these n-HA-coated magnetic nanoparticles are promising biomagnetic materials for future applications.

Effect of Temperature on Fe3O4 Magnetic Nanoparticles Prepared by Coprecipitation Method

2014 ◽  
Vol 900 ◽  
pp. 172-176 ◽  
Author(s):  
Ji Mei Niu ◽  
Zhi Gang Zheng
Keyword(s):  
Magnetic Nanoparticles ◽  
Coprecipitation Method ◽  
Effect Of Temperature ◽  
Vibrating Sample Magnetometer ◽  
X Ray Diffraction ◽  
X Ray ◽  
Magnetic Carriers ◽  
Average Grain Size ◽  
Or Gene ◽  
Block Temperature

The Fe3O4 magnetic nanoparticles obtained by the aqueous coprecipitation method are characterized systematically using scanning electron microscope, X-ray diffraction and vibrating sample magnetometer. These magnetic nanoparticles are spheric, dispersive, and have average grain size of 50 nm. The size and magnetic properties of Fe3O4 nanoparticles can be tuned by the reaction temperature. All samples exhibit high saturation magnetization (Ms=53.4 emu·g-1) and superparamagnetic behavior with a block temperature (TB) of 215K. These properties make such Fe3O4 magnetic nanoparticles worthy candidates for the magnetic carriers of targeted-drug or gene therapy in future.

A study on using expanded perlite with hydroxyapatite: Reinforced bio-composites

2021 ◽  
pp. 095441192199656
Author(s):  
Erdoğan Karip ◽  
Mehtap Muratoğlu
Keyword(s):  
Body Fluid ◽  
Xrd Analysis ◽  
Cold Isostatic Pressing ◽  
Expanded Perlite ◽  
X Ray Diffraction ◽  
Pressing Method ◽  
X Ray ◽  
Infra Red ◽  
Ft Ir

People are exposed to different kinds of diseases or various accidents in life. Hydroxyapatite (HA) has been widely employed for bone treatment applications. In this study, HA was extracted from sheep bones. Bio-composites were doped with 1, 5, and 10 wt.% of expanded perlite and 5 wt.% of ZrO2–MgO-P2O5. The bio-composites were prepared by the cold isostatic pressing method (250 MPa) and sintered at 900°C for 1 h. In order to evaluate the characteristics of the bio-composites, microhardness, density, X-ray diffraction (XRD), Fourier transform infra-red spectroscopy (FT-IR), scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS) analyses were carried out on them. Additionally, the specimens whose characteristics were determined were kept in synthetic body fluid (SBF), and their in vitro behavior was examined. As a result, it was observed that microhardness increased as both the weight and the grain size of the expanded perlite were increased. Calcium silicate, tri-calcium phosphate, and hydroxyapatite were observed in the XRD analysis of all samples, and the formation of apatite structures was increased by addition of ZrO2–MgO–P2O5.

Fabrication and evaluation of calcium phosphate cement scaffold with controlled internal channel architecture and complex shape

2007 ◽  
Vol 221 (8) ◽  
pp. 951-958 ◽  
Author(s):  
X Li ◽  
D Li ◽  
B Lu ◽  
L Wang ◽  
Z Wang
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Complex Shape ◽  
X Ray Diffraction ◽  
X Ray ◽  
Precise Control ◽  
Internal Channel ◽  
Channel Architecture

The ability to have precise control over internal channel architecture, porosity, and external shape is essential for tissue engineering. The feasibility of using indirect stereo-lithography (SL) to produce scaffolds from calcium phosphate cement materials for bone tissue engineering has been investigated. The internal channel architecture of the scaffolds was created by removal of the negative resin moulds made with SL. Scanning electron microscopy (SEM) showed highly open, well-interconnected channel architecture. The X-ray diffraction examination revealed that the hydroxyapatite phase formed at room temperature in the cement was basically stable up to 850 °C. There was no phase decomposition of hydroxyapatite, although the crystallinity and grain size were different. The ability of resulting structure to support osteoblastic cells culture was tested in vitro. Cells were evenly distributed on exterior surfaces and grew into the internal channels of scaffolds. To exploit the ability of this technique, anatomically shaped femoral supracondylar scaffolds with 300-800 μm interconnected channels were produced and characterized.

A Novel Missense Mutation (p.P52R) in Amelogenin Gene Causing X-linked Amelogenesis Imperfecta

2007 ◽  
Vol 86 (1) ◽  
pp. 69-72 ◽  
Author(s):  
M. Kida ◽  
Y. Sakiyama ◽  
A. Matsuda ◽  
S. Takabayashi ◽  
H. Ochi ◽  
...  
Keyword(s):  
Novel Mutation ◽  
Dental Enamel ◽  
Fluorescence Analysis ◽  
Hereditary Disease ◽  
Amelogenesis Imperfecta ◽  
X Ray Diffraction ◽  
Japanese Family ◽  
X Ray ◽  
Amelogenin Gene

Amelogenesis imperfecta (AI) is a hereditary disease with abnormal dental enamel formation. Here we report a Japanese family with X-linked AI transmitted over at least four generations. Mutation analysis revealed a novel mutation (p.P52R) in exon 5 of the amelogenin gene. The mutation was detected as heterozygous in affected females and as hemizygous in their affected father. The affected sisters exhibited vertical ridges on the enamel surfaces, whereas the affected father had thin, smooth, yellowish enamel with distinct widening of inter-dental spaces. To study the pathological cause underlying the disease in this family, we synthesized the mutant amelogenin p.P52R protein and evaluated it in vitro. Furthermore, we studied differences in the chemical composition between normal and affected teeth by x-ray diffraction analysis and x-ray fluorescence analysis. We believe that these results will greatly aid our understanding of the pathogenesis of X-linked AI.

THE STUDY ON GRANTING BIOACTIVITY OF Ti ALLOY BY SURFACE TREATMENT

2010 ◽  
Vol 17 (02) ◽  
pp. 153-157 ◽  
Author(s):  
N. R. HA ◽  
Z. X. YANG ◽  
G. C. KIM ◽  
K. H. HWANG ◽  
D. S. SEO ◽  
...  
Keyword(s):  
Surface Treatment ◽  
Body Fluid ◽  
Surface Effect ◽  
In Vitro Test ◽  
Ti Alloy ◽  
X Ray Diffraction ◽  
X Ray ◽  
Chemical Surface ◽  
Vitro Test

Titanium alloys are superior of biocompatibility, mechanical properties and chemical stability. The biocompatibility of Ti alloy is related to the surface effect between human tissue and implant. Therefore, the purpose of this study is to investigate the bioactivity of Ti alloy by alkali and acid chemical surface treatment; and the biocompatibility of Ti alloy was evaluated by in vitro test. Higher bone-bonding ability and bioactivity of the substrate were obtained by the formation of apatite layers on the Ti alloy in simulated body fluid. The microstructures of apatite layer were investigated by scanning electron microscope (SEM) and the formed phases were analyzed with X-ray diffraction (XRD).

Mesenchymal Stem Cell Response to Surface Altered Porous Ti

2015 ◽  
Vol 638 ◽  
pp. 67-72
Author(s):  
Ana Maria Salantiu ◽  
Florin Popa ◽  
Petru Pascuta ◽  
Olga Soritau ◽  
Noemi Dirzu ◽  
...  
Keyword(s):  
Stem Cell ◽  
X Ray Diffraction ◽  
X Ray ◽  
Chemically Modified ◽  
Porous Ti ◽  
Average Porosity ◽  
Ft Ir ◽  
Cell Testing ◽  
Scanning Electron

This work aims to investigate the influence of surface conditioning of porous Ti for enhancing its biological activity, as assessed by in vitro stem cell testing. Porous Ti samples with an average porosity of 32% were processed by Powder Metallurgy with dextrin as a space holder. The samples were subjected to H2O2 treatment to form an enhanced TiO2 film, followed by a heat treatment at 400°C and 600°C aiming to the crystallization of the as-formed amorphous titanium oxide. Samples characterization was performed by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FT-IR) and X-Ray Diffraction (XRD). The treated surfaces revealed to be made of both anatase and rutile TiO2, with groove–shaped structure and cracks on the surface of the TiO2 film. The intrinsic biocompatibility of the chemically modified porous Ti surfaces was assessed in vitro. In our cell culture tests, stem cells were found to attach and proliferate better on the chemically treated Ti surfaces compared to the control untreated Ti surfaces.

Potent Anticancer Activities of Beauvericin against KB cells In Vitro by Inhibiting the Expression of ACAT1 and Exploring Binding Affinity

2021 ◽  
Vol 21 ◽  
Author(s):  
Haiming Zhou ◽  
Jing Zhang ◽  
Xiaoqing Chen ◽  
Shili Guo ◽  
Huimei Lin ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Cell Line ◽  
Binding Affinity ◽  
X Ray Diffraction ◽  
Kb Cells ◽  
Anticancer Activities ◽  
Monoclinic Crystal ◽  
X Ray ◽  
Multiple Tumor

Background and Objective: Beauvericin (BEA), a cyclic hexadepsipeptide mycotoxin, is a potent inhibitor of the acyl-CoA: cholesterol acyltransferase enzyme 1 (ACAT1) which involved in multiple tumor-correlated pathways. However, the binding mechanisms between BEA and ACAT1 were not elucidated. Methods: BEA was purified from a mangrove entophytic Fusarium sp. KL11. Single-crystal X-ray diffraction was used to determine the structure of BEA. Wound healing assays of BEA against KB cell line and MDA-MB-231 cell line were evaluated. Inhibitory potency of BEA against ACAT1 was determined by ELISA assays. Molecular docking was carried out to illuminate the bonding mechanism between BEA and ACAT1. Results: The structure of BEA was confirmed by X-ray diffraction, indicating a monoclinic crystal system with P21 space group (α = 90°, β = 92.2216(9)o, γ= 90o). BEA displayed migration-inhibitory activities against KB cells and MDA-MB-231 cells in vitro. ELISA assays revealed the protein expression level of ACAT1 in KB cells was significantly decreased after BEA treatment (P ＜0.05). Molecular docking demonstrated that BEA formed hydrogen bond with His425 and pi-pi staking with Tyr429 in ACAT1. Conclusions: BEA sufficiently inhibited the proliferation and migration of KB cells and MDA-MB-231 cells by downregulating ACAT1 expression. In addition, BEA potentially possessed a strong binding affinity with ACAT1. BEA may serve as a potential lead compound for the development of a new ACAT1-targeted anticancer drug.

Effects of dietary alteration of bicarbonate and magnesium on rat bone

1986 ◽  
Vol 250 (2) ◽  
pp. F302-F307 ◽  
Author(s):  
J. M. Burnell ◽  
C. Liu ◽  
A. G. Miller ◽  
E. Teubner
Keyword(s):  
Crystal Structure ◽  
Bone Formation ◽  
X Ray Diffraction ◽  
X Ray ◽  
Growing Rats ◽  
Final Composition ◽  
Rat Bone

To study the effects of bicarbonate and magnesium on bone, mild acidosis and/or hypermagnesemia were produced in growing rats by feeding ammonium chloride and/or magnesium sulfate. Bone composition, quantitative histomorphometry, and mineral x-ray diffraction (XRD) characteristics were measured after 6 wk of treatment. The results demonstrated that both acidosis (decreased HCO3) and hypermagnesemia inhibited periosteal bone formation, and, when combined, results were summative; and the previously observed in vitro role of HCO3- and Mg2+ as inhibitors of crystal growth were confirmed in vivo. XRD measurements demonstrated that decreased plasma HCO3 resulted in larger crystals and increased Mg resulted in smaller crystals. However, the combined XRD effects of acidosis and hypermagnesemia resembled acidosis alone. It is postulated that the final composition and crystal structure of bone are strongly influenced by HCO3- and Mg2+, and the effects are mediated by the combined influence on both osteoblastic bone formation and the growth of hydroxyapatite.

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