Nanomechanical Measurement of Bacterial Adhesion Force Using Soft Nanopillars

2017 ◽  
Vol 17 (11) ◽  
pp. 7966-7970 ◽  
Author(s):  
Naeun Oh ◽  
Myeongjun Jun ◽  
Jaejong Lee ◽  
Chi-Yong Eom ◽  
Sungsu Park
Keyword(s):  
Bacterial Adhesion ◽  
Adhesion Force

scholarly journals Assessing the Functional Properties of TiZr Nanotubular Structures for Biomedical Applications, through Nano-Scratch Tests and Adhesion Force Maps

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 900
Author(s):  
Maria Vardaki ◽  
Aida Pantazi ◽  
Ioana Demetrescu ◽  
Marius Enachescu
Keyword(s):  
Surface Roughness ◽  
Functional Properties ◽  
Bacterial Adhesion ◽  
Biomedical Applications ◽  
Adhesion Force ◽  
Roughness Parameters ◽  
Force Microscopy ◽  
Atomic Force ◽  
The Mean ◽  
Scratch Tests

In this work we present the results of a functional properties assessment via Atomic Force Microscopy (AFM)-based surface morphology, surface roughness, nano-scratch tests and adhesion force maps of TiZr-based nanotubular structures. The nanostructures have been electrochemically prepared in a glycerin + 15 vol.% H2O + 0.2 M NH4F electrolyte. The AFM topography images confirmed the successful preparation of the nanotubular coatings. The Root Mean Square (RMS) and average (Ra) roughness parameters increased after anodizing, while the mean adhesion force value decreased. The prepared nanocoatings exhibited a smaller mean scratch hardness value compared to the un-coated TiZr. However, the mean hardness (H) values of the coatings highlight their potential in having reliable mechanical resistances, which along with the significant increase of the surface roughness parameters, which could help in improving the osseointegration, and also with the important decrease of the mean adhesion force, which could lead to a reduction in bacterial adhesion, are providing the nanostructures with a great potential to be used as a better alternative for Ti implants in dentistry.

Nanoprobe-based force spectroscopy as a versatile platform for probing the mechanical adhesion of bacteria

Nanoscale ◽  
2019 ◽  
Vol 11 (16) ◽  
pp. 7648-7655 ◽  
Author(s):  
Chanchan Yu ◽  
Di Zhang ◽  
Xueyan Feng ◽  
Yahong Chai ◽  
Pan Lu ◽  
...  
Keyword(s):  
Bacterial Adhesion ◽  
Adhesion Force ◽  
Force Spectroscopy ◽  
Substrate Stiffness ◽  
Mechanical Adhesion

Nanoprobe-based force spectroscopy was developed as a new platform to investigate how substrate stiffness regulates the bacterial adhesion force.

scholarly journals Emergent heterogeneous microenvironments in biofilms: substratum surface heterogeneity and bacterial adhesion force-sensing

2018 ◽  
Vol 42 (3) ◽  
pp. 259-272 ◽  
Author(s):  
Yijin Ren ◽  
Can Wang ◽  
Zhi Chen ◽  
Elaine Allan ◽  
Henny C van der Mei ◽  
...  
Keyword(s):  
Bacterial Adhesion ◽  
Adhesion Force ◽  
Surface Heterogeneity ◽  
Force Sensing

scholarly journals Strength of bacterial adhesion on nanostructured surfaces quantified by substrate morphometry

Nanoscale ◽  
2019 ◽  
Vol 11 (42) ◽  
pp. 19713-19722 ◽  
Author(s):  
Christian Spengler ◽  
Friederike Nolle ◽  
Johannes Mischo ◽  
Thomas Faidt ◽  
Samuel Grandthyll ◽  
...  
Keyword(s):  
Cell Wall ◽  
Surface Area ◽  
Bacterial Adhesion ◽  
Smooth Surface ◽  
Adhesion Force ◽  
Nanostructured Surfaces

Bacterial adhesion to nanostructured surfaces can be quantified by surface morphometry: the surface area that is accessible in a certain depth for tethering cell wall molecules equals the fraction of adhesion force as compared to a smooth surface.

Thermal Treatments Modulate Bacterial Adhesion to Dental Enamel

2011 ◽  
Vol 90 (12) ◽  
pp. 1451-1456 ◽  
Author(s):  
X.L. Hu ◽  
B. Ho ◽  
C.T. Lim ◽  
C.S. Hsu
Keyword(s):  
Thermal Treatment ◽  
Zeta Potential ◽  
Physicochemical Properties ◽  
Bacterial Adhesion ◽  
Laser Scanning ◽  
Adhesion Force ◽  
Bacterial Species ◽  
Dental Enamel ◽  
Laser Scanning Microscopy ◽  
Confocal Laser

Numerous studies have demonstrated the effects of laser-induced heat on demineralization of enamel; however, no studies have investigated the link between heat/laser-induced changes in physicochemical properties and bacterial adhesion. In this study, we investigated the effects of thermal treatment on surface properties of enamel such as hydrophobicity and zeta potential. Bacterial adhesion to treated surfaces was characterized by confocal laser scanning microscopy, and adhesion force was quantified by atomic force microscopy. The hydrophobicity of enamel increased after heating (p < 0.05), and the zeta potential of heated enamel became more negative than that of the control (p < 0.01). Streptococcus oralis and S. mitis were more hydrophilic than S. sanguis, with more negative zeta potential (all p < 0.01). S. mitis and S. oralis occupied significantly less area on enamel after being heated (p < 0.05). Heating reduced the adhesion force of both S. mitis and S. oralis to enamel with or without saliva coating. Reduction of adhesion force was statistically significant for S. mitis (p < 0.01), whereas that of S. oralis was not statistically significant (p > 0.05). Heating did not affect the adhesion of S. sanguis with or without saliva coating. In conclusion, thermal treatment and photothermal/laser treatments may modulate the physicochemical properties of enamel, preventing the adhesion of some bacterial species.

scholarly journals Nanoscale Cell Wall Deformation Impacts Long-Range Bacterial Adhesion Forces on Surfaces

2013 ◽  
Vol 80 (2) ◽  
pp. 637-643 ◽  
Author(s):  
Yun Chen ◽  
Akshay K. Harapanahalli ◽  
Henk J. Busscher ◽  
Willem Norde ◽  
Henny C. van der Mei
Keyword(s):  
Cell Wall ◽  
Long Range ◽  
Bacterial Adhesion ◽  
Bacterial Cell ◽  
Adhesion Force ◽  
Bacterial Cell Wall ◽  
Nanomechanical Property ◽  
Adhesion Forces ◽  
Bacterial Strains ◽  
Content Type

ABSTRACTAdhesion of bacteria occurs on virtually all natural and synthetic surfaces and is crucial for their survival. Once they are adhering, bacteria start growing and form a biofilm, in which they are protected against environmental attacks. Bacterial adhesion to surfaces is mediated by a combination of different short- and long-range forces. Here we present a new atomic force microscopy (AFM)-based method to derive long-range bacterial adhesion forces from the dependence of bacterial adhesion forces on the loading force, as applied during the use of AFM. The long-range adhesion forces of wild-typeStaphylococcus aureusparent strains (0.5 and 0.8 nN) amounted to only one-third of these forces measured for their more deformable isogenic Δpbp4mutants that were deficient in peptidoglycan cross-linking. The measured long-range Lifshitz-Van der Waals adhesion forces matched those calculated from published Hamaker constants, provided that a 40% ellipsoidal deformation of the bacterial cell wall was assumed for the Δpbp4mutants. Direct imaging of adhering staphylococci using the AFM peak force-quantitative nanomechanical property mapping imaging mode confirmed a height reduction due to deformation in the Δpbp4mutants of 100 to 200 nm. Across naturally occurring bacterial strains, long-range forces do not vary to the extent observed here for the Δpbp4mutants. Importantly, however, extrapolating from the results of this study, it can be concluded that long-range bacterial adhesion forces are determined not only by the composition and structure of the bacterial cell surface but also by a hitherto neglected, small deformation of the bacterial cell wall, facilitating an increase in contact area and, therewith, in adhesion force.

Bacterial adhesion force quantification by fluidic force microscopy

Nanoscale ◽  
2015 ◽  
Vol 7 (9) ◽  
pp. 4070-4079 ◽  
Author(s):  
Eva Potthoff ◽  
Dario Ossola ◽  
Tomaso Zambelli ◽  
Julia A. Vorholt
Keyword(s):  
Single Cell ◽  
Bacterial Adhesion ◽  
Adhesion Force ◽  
State Of The Art ◽  
Force Spectroscopy ◽  
Force Microscopy ◽  
Cell Force ◽  
Serial Measurements ◽  
Cell Fixation

Fluidic force microscopy demonstrates the potential to quantify bacterial adhesion by single-cell force spectroscopy, achieving higher immobilization forces than state-of-the-art cell-cantilever interactions. Reversible cell fixation on the tip allows for serial measurements of many cells in the nN range using a single cantilever.

Bacteria Adhesion on Polydimethylsiloxane Surfaces Impacted by Material Viscoelasticity or Surface Chemistry?

2020 ◽  
Author(s):  
Fei Pan ◽  
Stefanie Altenried ◽  
Mengdi Liu ◽  
Dirk Hegemann ◽  
Ezgi Bülbül ◽  
...  
Keyword(s):  
Bacterial Adhesion ◽  
Adhesion Force ◽  
Bulk Material ◽  
Bacterial Colonization ◽  
Chemical Properties ◽  
Model Material ◽  
Interfacial Chemistry ◽  
Material Stiffness ◽  
Bacteria Adhesion ◽  
The Impact

&lt;p&gt;&lt;strong&gt;Among nosocomial infections, materials associated infections are the most frequent and severe due to biofilm formation. To prevent bacterial colonization, understanding the underlying interaction between bacteria and surface is fundamental. Herein we focused on studying how material viscoelasticity and physicochemistry can influence bacterial adhesion, using polydimethylsiloxane (PDMS) as a model material. To delineate the impact caused by bulk material from interfacial physicochemical properties, a 2 nm PDMS-like polymer layer was coated onto PDMS surfaces of different stiffness to confer comparable surface chemical properties, while retaining similar viscoelasticity for coated and uncoated PDMS species. Although the uncoated samples displayed increasing interfacial adhesion force with the decreasing Young's modulus, the nanolayer coating ensured comparable forces independent of material stiffness. The Gram negative strains Escherichia coli and Pseudomonas aeruginosa and the Gram positive strain Staphylococcus epidermidis were found to adhere respectively in similar numbers on the coated surfaces of different PDMS species, whereas the amount on the uncoated surfaces increased several fold with the decreasing modulus. The similar adhesion behaviour was noticed for abiotic polystyrene beads of similar size to bacteria, demonstrating that the interfacial chemistry of the PDMS rather than the material viscoelasticity plays a crucial role in bacterial adhesion.&lt;/strong&gt;&amp;#160;&lt;/p&gt;

scholarly journals Hydroxyapatite pellets as versatile model surfaces for systematic studies on enamel

2021 ◽  
Author(s):  
Johannes Mischo ◽  
Thomas Faidt ◽  
Ryan B. McMillan ◽  
Johanna Dudek ◽  
Gubesh Gunaratnam ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Bacterial Adhesion ◽  
Adhesion Force ◽  
Experimental Studies ◽  
Medical Application ◽  
Good Alternative ◽  
Adhesion Properties ◽  
Natural Materials ◽  
Rupture Length ◽  
Composition Structure

AbstractResearch into materials for medical application draws inspiration from naturally occurring or synthesized surfaces, just like many other research directions. For medical application of materials, particular attention has to be paid to biocompatibility, osseointegration and bacterial adhesion behavior. To understand their properties and behavior, experimental studies with natural materials such as teeth are strongly required. The results, however, may be highly case-dependent because natural surfaces have the disadvantage of being subject to wide variations, for instance in their chemical composition, structure, morphology, roughness, and porosity. A synthetic surface which mimics enamel in its performance with respect to bacterial adhesion and biocompatibility would, therefore, facilitate systematic studies much better. In this study, we discuss the possibility of using hydroxyapatite (HAp) pellets to simulate the surfaces of teeth and show the possibility and limitations of using a model surface. We performed single-cell force spectroscopy with single Staphylococcus aureus cells to measure adhesion-related parameters such as adhesion force and rupture length of adhesins binding to HAp and enamel. We also examine the influence of blood plasma and saliva on the adhesion properties of S. aureus. The results of these measurements are matched to water wettability, elemental composition of the samples and the change in the macromolecules adsorbed over time. We found that the adhesion properties of S. aureus were similar on both samples under all conditions: Significant decreases in adhesion strength were found equally in the presence of saliva or blood plasma on both surfaces. We therefore conclude that HAp pellets are a good alternative for natural dental material. This is especially true when slight variations in the physicochemical properties of the natural materials may affect the experimental series.

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