Preparation of Novel Bi-Modal Nano Contrast Agent and Its Application in Diagnosis of Breast Cancer

2021 ◽  
Vol 13 (9) ◽  
pp. 1603-1610
Author(s):  
Shi Zhang ◽  
Meizhen Fan ◽  
Yan Wang ◽  
Zhiyong Zhan ◽  
Ying Hu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Contrast Agent ◽  
Average Particle Size ◽  
Ultrasound Contrast Agent ◽  
Phase Changes ◽  
Cell Group ◽  
Average Particle ◽  
Lymph Node Imaging ◽  
Lymphatic Imaging ◽  
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In the study, a new ultrasound contrast agent was used in the above diagnosis. Specifically, PLGA nanoparticles (PNA) loaded with Au nanorods and perfluorohexane (PFH) were prepared by the double emulsification method, and then the aptamer (APT) and PNA were connected by the carbodiimide method to obtain dualmodal ultrasound/photoacoustic targeting contrast agent (APT-PNA). Subsequently, physical characterization was performed on the prepared material, and then a fluorescence microscope was used to detect in vitro binding to breast cancer MCF-7 cells, with the normal nanoparticle group, HELA cell group, and APT interference group as controls. On this basis, it was used for the SLNB of breast cancer patients. The results showed that, the average particle size of APT-PNA was (468.5±23.6) nm, and the connection rate between the APT and the PNA reached 95.68%. After laser irradiation, the photo-induced phase changes of the APT-PNA were obvious. The APT-PNA aggregated around and bound firmly to breast cancer MCF-7 cells, while other control groups did not show obvious specific binding. After using APT-PNA, the ultrasound signal and photoacoustic signal were obviously enhanced than before irradiation. Of the 40 patients who participated in the trial, 37 developed lymphatic imaging, with an imaging rate of 92.5%, and 32 of the 37 patients with lymphatic imaging developed lymph node imaging, with a success rate of 86.5%.

Novel Difolate Targeting Nano-Level Ultrasound Contrast Agent for Therapy of Breast Cancer Tumor Cells

2021 ◽  
Vol 13 (7) ◽  
pp. 1295-1303
Author(s):  
Guangheng Liu ◽  
Xiangfeng Yang ◽  
Qiming Niu ◽  
Wenkui Sun
Keyword(s):  
Breast Cancer ◽  
Contrast Agent ◽  
Tumor Cells ◽  
Folate Receptor ◽  
Ultrasound Contrast Agent ◽  
Hydroxyl Group ◽  
Binding Ability ◽  
Ultrasound Contrast ◽  
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ABSTRACTA new type of difolate targeting nano-level ultrasound contrast agent ((folate molecule, FOL)2-TUAs) was prepared, so as to investigate its targeted binding effect with human breast cancer mammary carcinoma cells (MCF-7) in vitro. L-2-aminoadipic acid (L-2-AD) as a branch unit was inserted at the hydroxyl end of distearoyl phosphatidylethanolamine (DISP)-PEG2000-COOH to construct a tree structure. At this time, the free hydroxyl group in the distearoyl phosphatidylethanolamine (DISP)-PEG2000-COOH structure modified the FOL with the help of N-Hydroxysuccinimide/N,N'-dicyclohexylcarbodiimide (NHS/DCC). Each 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DISP-PEG2000) connected two FOLs to generate difolate targeted nanomaterials. Nano laser particle size (PS) and Zeta potential analyzer (ZPA) were applied to analyze the physical characteristics of the material such as PS and dispersion, and the enhanced development effect in vitro was detected by the ultrasonic diagnostic instrument. Besides, the targeted binding ability of the contrast agent based on this material to folate receptor (FR) overexpressing MCF-7 cells was analyzed by flow cytometry (FCM) and fluorescence microscope. In the experiment, hydrogen-1 nuclear magnetic resonance (1H NMR) demonstrated that (FOL)2-TUAs was successfully synthesized. The surface of this material was round and uniformly distributed without aggregation. According to the relative number of FOL molecules, non-targeted nano-agent (U-TUA), monofolate targeted nano-agent (FOL-TUA), and difolate targeted nano-agent ((FOL)2-TUA) were obtained. The in vitro imaging showed that different materials exhibited enhanced imaging effects in ultrasonic diagnostic equipment. FCM and fluorescence microscopy both indicated that the difolate TUA could achieve a good binding to MCF-7 cells. Most of the nano-agents were attached to the cell membrane, surrounded by red fluorophore, namely increasing the FOL content of DISP-PEG2000 chain could enhance the targeted binding ability of tumor cells.

Catechin Loaded Poly(lactic-co-glycolic acid) Nanoparticles: Characterization, Antioxidant and Cytotoxic Activity Against MCF-7 Breast Cancer Cells

2020 ◽  
Vol 20 (9) ◽  
pp. 5313-5321
Author(s):  
Burcu Turkoglu ◽  
Banu Mansuroglu
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Encapsulation Efficiency ◽  
Average Particle Size ◽  
Antioxidant Properties ◽  
Breast Cancer Cell Lines ◽  
Reaction Yield ◽  
Average Particle ◽  
Dpph Method ◽  
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Here we evaluate the cytotoxic and antioxidant properties of Catechin (Cat) loaded nanoparticles (CatNps) on breast cancer cell lines. CatNps were prepared by the modified single emulsion solvent evaporation method. The structural, physical and chemical properties of the CatNps including PLGA-Cat interactions, and surface characteristics, were analyzed by dynamic light scattering (DLS), Fourier transform infrared (FT-IR), and Scanning Electron Microscopy (SEM). The average particle size of CatNps was 190.5±1.762 nm with an encapsulation efficiency of 10% and zeta potential value of 13.7±1.258 mV. The CatNps had reaction yield of 43.47±1.1% and loading capacity of 3.710±2.6% Treating MCF-7 cells with CatNps for 48 h led to a decrease in cell viability with an of IC50 22.59 μg/mL. The antioxidant behavior of CatNps was evaluated by the DPPH method in various pH environments (pH 3-, 6-, 7.4-, 9- and 12). Despite the low encapsulation efficiency, CatNps showed significant antioxidant efficacy against DPPH radicals relative to Cat. Our results revealed that CatNps could be used as promising anti-cancer agents due to their enhanced cytotoxic activity, increased bioavailability, and antioxidant properties.

scholarly journals Evaluation of Microwave Ablation in 4T1 Breast Tumor by a Novel VEFGR2 Targeted Ultrasound Contrast Agents

2021 ◽  
Vol 11 ◽  
Author(s):  
Xiaoyu Li ◽  
Shujun Xia ◽  
Ri Ji ◽  
Weiwei Zhan ◽  
Wei Zhou
Keyword(s):  
Contrast Agent ◽  
Microwave Ablation ◽  
Average Particle Size ◽  
Ultrasound Contrast Agent ◽  
Ultrasound Contrast Agents ◽  
Tumor Ablation ◽  
Average Particle ◽  
Ultrasound Contrast ◽  
Ultrasound Signal

ObjectivesA novel ultrasound contrast agent (UCA) VEGFR2-targeting iron-doped silica (SiO2) hollow nanoparticles (VEGFR2-PEG-HSNs-Fe NPs) was prepared and applied in microwave ablation for breast cancer to investigate its value in the evaluation of effectiveness after tumor ablation.MethodsVEGFR2-PEG-HSNs-Fe NPs were prepared by using nano-SiO2, which was regarded as a substrate and etched by ferrous acetate, and then modified with anti-VEGFR2 antibody. Laser confocal microscope and flow cytometry were used to observe its main physicochemical properties, and biological safety was also investigated. After the xenograft tumor was treated with microwave ablation, the extent of perfusion defect was evaluated by ultrasound by injecting VEGFR2-PEG-HSNs-Fe NPs.ResultsThe average particle size of VEGFR2-PEG-HSNs-Fe was 276.64 ± 30.31 nm, and the surface potential was −13.46 ± 2.83 mV. In vitro, the intensity of ultrasound signal increased with UCA concentration. Good biosafety was performed in in vivo and in vitro experiments. The enhanced ultrasound signal was detected in tumors after injection of VEGFR2-PEG-HSNs-Fe NPs, covering the whole tumor. The lesions, which were incompletely ablated, presented as contrast agent perfusion at the periphery of the tumor, and contrast enhanced ultrasound (CEUS) was performed again after complementary ablation. It was confirmed that all the lesions were completely ablated.ConclusionNano-targeted UCAs VEGFR2-PEG-HSNs-Fe NPs had good biosafety and ability of specific imaging, which might be used as a contrast agent in CEUS to evaluate the efficacy of tumor ablation.

scholarly journals Synthesis and Characterization of Silver Nanoparticles Using Cannonball Leaves and Their Cytotoxic Activity against MCF-7 Cell Line

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Preetha Devaraj ◽  
Prachi Kumari ◽  
Chirom Aarti ◽  
Arun Renganathan
Keyword(s):  
Silver Nanoparticles ◽  
Cell Line ◽  
Average Particle Size ◽  
Cancer Cell Line ◽  
Silver Ions ◽  
Average Particle ◽  
Surface Plasmon Resonance Peak ◽  
Plasmon Resonance Peak ◽  
Vis Spectroscopy ◽  
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Cannonball (Couroupita guianensis) is a tree belonging to the family Lecythidaceae. Various parts of the tree have been reported to contain oils, keto steroids, glycosides, couroupitine, indirubin, isatin, and phenolic substances. We report here the synthesis of silver nanoparticles (AgNPs) using cannonball leaves. Green synthesized nanoparticles have been characterized by UV-Vis spectroscopy, SEM, TEM, and FTIR. Cannonball leaf broth as a reducing agent converts silver ions to AgNPs in a rapid and ecofriendly manner. The UV-Vis spectra gave surface plasmon resonance peak at 434 nm. TEM image shows well-dispersed silver nanoparticles with an average particle size of 28.4 nm. FTIR showed the structure and respective bands of the synthesized nanoparticles and the stretch of bonds. Green synthesized silver nanoparticles by cannonball leaf extract show cytotoxicity to human breast cancer cell line (MCF-7). Overall, this environmentally friendly method of biological silver nanoparticles production provides rates of synthesis faster than or comparable to those of chemical methods and can potentially be used in various human contacting areas such as cosmetics, foods, and medical applications.

scholarly journals Characteristics of Nanophase WC and WC-3 wt% (Ni, Co, and Fe) Alloys Using a Rapid Sintering Process for the Application of Friction Stir Processing Tools

2015 ◽  
Vol 2015 ◽  
pp. 1-9
Author(s):  
Daeup Kim ◽  
Young Choi ◽  
Yongil Kim ◽  
Seungboo Jung
Keyword(s):  
Friction Stir Processing ◽  
Average Particle Size ◽  
Milling Process ◽  
Phase Changes ◽  
Average Particle ◽  
Sintering Process ◽  
Friction Stir ◽  
Plasma Sintering ◽  
Spark Plasma ◽  
Rapid Sintering

Microstructures and mechanical characteristics of tungsten carbide- (WC-) based alloys, that is, WC, WC-3 wt% Ni, WC-3 wt% Co, and WC-3 wt% Fe, fabricated using a spark plasma sintering (SPS) method for the application of friction stir processing tools were evaluated. The sintered bodies with a diameter of 66 mm showed relative densities of up to 99% with an average particle size of 0.26~0.41 μm under a pressure condition of 60 MPa with an electric current for 35 min without noticeable grain growth during sintering. Even though no phase changes were observed after the ball milling process the phases of W2C andWC1-xappeared in all sintered samples after sintering. The Vickers hardness and fracture toughness of the WC, WC-3 wt% Ni, WC-3 wt% Co, and WC-3 wt% Fe samples ranged from 2,240 kg mm2to 2,730 kg mm2and from 6.3 MPa·m1/2to 9.1 MPa·m1/2, respectively.

scholarly journals Cascade Release Nanocarriers for the Triple-Negative Breast Cancer Near-Infrared Imaging and Photothermal-Chemo Synergistic Therapy

2021 ◽  
Vol 11 ◽  
Author(s):  
Ke Li ◽  
Ruyue Li ◽  
Baona Zhou ◽  
Jing Chen ◽  
Kai Lan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Triple Negative Breast Cancer ◽  
Near Infrared ◽  
Triple Negative ◽  
Infrared Imaging ◽  
Average Particle Size ◽  
Average Particle ◽  
Near Infrared Imaging ◽  
Synchronous Tumor ◽  
Treatment Approaches

Triple-negative breast cancer (TNBC) has inadequate treatment approaches and a poor prognosis. It is urgent to develop new treatment approaches for TNBC. The combination of photothermal therapy (PTT) and chemotherapy is a very effective potential therapy for TNBC. However, asynchronous accumulation, unclear efficacy, and toxic side effects hinder the further promotion of this method. Therefore, we designed and constructed a new type of nanocarriers, the cascade release near-infrared imaging (NIFI) & thermal-chemo combination nanoparticles (CNC NPs), that can release drugs through the cascade of ultrasound triggering and pH responding to achieve the synchronous tumor accumulation, monitoring and synergistic treatment of two functional molecules. The key material of CNC NPs is the polydopamine (PDA), which, through self-assembling, forms a rigid shell that contains doxorubicin (DOX) and NIF fluorescent dye IR780 on the surface of the perfluorohexane (PFH) microbubbles. The results show that CNC NPs have a hollow core-shell structure with an average particle size of 97.3 ± 27.2 nm and have exceptional colloidal stability and photothermal conversion efficiency. The NPs can effectively perform cascade drug release through ultrasound triggering and pH responding. CNC NPs have good in vivo biological safety and excellent fluorescence imaging, drug delivery, and therapeutic abilities in the TNBC models. These results provide an experimental basis for the development of new clinical treatment methods for TNBC.

Effect of farrerol on the proliferation of estrogen receptor-positive breast cancer by downregulating oncogenic genes with estrogen receptor response elements.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e12541-e12541
Author(s):  
Xiao-Qing Lu ◽  
Rui Yang ◽  
Jia-Qian Zhang ◽  
Shengxiao Zhang ◽  
Huan-Hu Zhang
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Molecular Docking ◽  
Mammary Epithelial Cells ◽  
Inhibitory Effect ◽  
Mammary Epithelial ◽  
Cell Group ◽  
Response Elements ◽  
Er Positive ◽  
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e12541 Background: Breast cancer (BC) is the most common malignant tumor among women worldwide, the most common of which is estrogen receptor (ER) -positive BC. Farrerol, a noval estrogen-like chemicals, is closely associated with ER positive BC. In this study, we will explore the anticancer effect of farrerol on ER-positive BC and the detailed mechanism. Methods: Cloning formation assay and MTT assay were used to evaluate the effect of farrerol on proliferation ofcells. The affinity of farrerol, estrogen and tamoxifen with ER was tested by molecular docking technique. Point mutation was performed on the possible binding sites of ER according to the results of virtual docking, and Surface Plasmon Resonance (SPR). ER response elements in BC cells with farrerol were evaluated by qPCR. Results: With the increased farrerol concentration, the number of cell clones formed gradually decreased and the volume of clones became smaller in the ER positive McF-7 cell group, while a completely opposite clones was observed in non- ER positive MDA-MB-231 cells. The proliferation inhibition rate of McF-7 cells in the 160 mol/L and 320 mol/L groups was 45.86% and 65.3% respectively, significantly higher than that of MDA-MB-231 and normal mammary epithelial cells (P < 0.05). Both molecular docking technique and SPR experiment showed that farrerol could bind to ER at different sites than estrogen binding. Fiarrerol effectively down-regulated the transcription of the oncogenes MYC and TFF1, and suppressed ER-positive BC. Conclusions: Farrerol has the opposite effect with estrogen, which inhibited the proliferation of ER-positive BC cells, and downregulated the expression of oncogenic genes with ER response elements in BC cells. Therefore, farrerol, as a natural ingredient, could be further developed and applied with its obvious inhibitory effect on ER-positive BC in the future.

Construction of long-circulation EpCAM targeted drug delivery system and its application in the diagnosis and treatment of breast cancer

2020 ◽  
pp. 088532822096513
Author(s):  
Chao Song ◽  
Chao Gao ◽  
Jing Zhao ◽  
Zhenxin Wang
Keyword(s):  
Breast Cancer ◽  
Drug Delivery ◽  
Drug Delivery System ◽  
Delivery System ◽  
Targeted Drug Delivery ◽  
Drug Carrier ◽  
Average Particle Size ◽  
Average Particle ◽  
Tumor Focus ◽  
Targeted Drug

Docetaxel (DOX) is usually one of drugs used for breast cancer treatment. The key of targeted drug delivery therapy is to deliver effective drugs directly and safely to the tumor focus via an efficient targeting drug carrier with immunogenicity. In this study, Long-circulating targeted drug carrying microspheres (DOX-PEG-EpCAM-MNs) entrapping DOX were constructed. In addition, both cytotoxicity and magnetic resonance imaging (MRI) analyses were performed to establish a mouse model and further complete corresponding performance analysis.The results showed that the average particle size of DOX-PEG-EpCAM-MNs was 139.3 ± 1.6 nm. Morphological analysis proves that they are spherical and uniformly dispersed. The Corresponding entrapment rate and drug carrying capacity are 82.43% and 7.16% respectively. Additionally, MRI shows that they have the capability to track tumor cells within 5 days. This study established a safe and efficient breast cancer cells targeted long-circulating drug delivery system.

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