Injectable Form of Cross-Linked Hyaluronan is Effective for Middle Ear Wound Healing

2007 ◽  
Vol 116 (9) ◽  
pp. 667-673 ◽  
Author(s):  
Simon Angeli ◽  
Sarah Connell ◽  
Brian Gibson ◽  
Ali Ozdek ◽  
John T. McElveen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Middle Ear ◽  
Otoacoustic Emissions ◽  
Inflammatory Cells ◽  
Control Group ◽  
Term Study ◽  
Distortion Product ◽  
Long Term Study ◽  
Group 2 ◽  
Group 1

Objectives: Two studies were designed to investigate a hyaluronan (HA) gel for middle ear (ME) wound healing. Methods: We used a guinea pig model of ME wound healing. In a long-term study, we performed a comparison of hearing and ME inflammation in 3 groups. Group 1 (n = 8) underwent bilateral wounding of ME mucosa and unilateral packing of the ME with HA gel (Sepragel). Group 2 (n = 6) was the same as group 1 except that the packing was absorbable bovine collagen sponges (Gelfoam). Group 3, the control group (n = 14), had operated, unpacked ears. In a short-term study, we investigated ME retention of HA gel at 1 and 2 weeks (n = 16). Results: At 1 week, all ears showed decreased distortion product otoacoustic emissions (DPOAEs) and auditory brain stem responses (ABRs) secondary to ME packing and postsurgical inflammation. The controls recovered preoperative DPOAEs and ABRs by week 2. Group 1 had decreased low-frequency DPOAEs at weeks 2 and 6, but their high-frequency DPOAEs and ABRs recovered to preoperative values by week 6. Group 2 had hearing losses that persisted throughout the study. Group 1 showed normal ME and inner ear histologic characteristics. Group 2 showed inflammatory cells within the ME and cochleas. Group 1 showed less packing retention than did group 2 at week 6 (p = 016). Eighty-five percent of the HA packing remained at 1 week, and 73% at 2 weeks. Conclusions: Hyaluronan gel was a relatively safe and effective ME packing material in our animal model.

scholarly journals A Prospective Randomized Study: The Usefulness and Efficacy of Negative Pressure Wound Therapy with Lipidocolloid Polyester Mesh Compared to Traditional Negative Pressure Wound Therapy for Treatment of Pressure Ulcers

Pharmaceutics ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 813
Author(s):  
Wooyeol Baek ◽  
Nara Lee ◽  
Eun Jin Han ◽  
Tai Suk Roh ◽  
Won Jai Lee
Keyword(s):  
Wound Healing ◽  
Pressure Ulcer ◽  
Negative Pressure ◽  
Negative Pressure Wound Therapy ◽  
Randomized Study ◽  
Contact Layer ◽  
Control Group ◽  
Wound Therapy ◽  
Group 2 ◽  
Group 1

To improve healing of pressure ulcer wounds, it is important to optimize the conditions of the area surrounding the wound. Negative pressure wound therapy (NPWT) promotes wound healing, however, the removal of NPWT can cause pain or focal bleeding, delaying wound healing or causing infection. In this study, we reviewed the efficacy of the lipidocolloid non-adherent dressing (Urgotul®) as a wound contact layer. A total of 38 patients from the same facility who applied NPWT from April 2016 to October 2019 were included and divided into two groups; NPWT with the lipidocolloid non-adherent dressing (group 1, experimental group, 19 patients) and NPWT only (group 2, control group, 19 patients). The condition of the wound was examined prior to NPWT application, at one week, and again at three weeks after application. No significant differences were found between groups for general characteristics, bacterial culture or photo analysis. However, when comparing groups based on the time of examination, there was a significant reduction of the wound size in group 1 (p = 0.001) but not in group 2 (p = 0.082). Therefore, the current study finds that using the lipidocolloid non-adherent dressing as a wound contact layer in NPWT stimulates healing by shrinking the size of the pressure ulcer wound.

scholarly journals ON IMPROVING THE TREATMENT OF PATIENTS WITH CHRONIC WOUNDS OF VENOUS GENESIS

2019 ◽  
Vol 19 (3) ◽  
pp. 75-78
Author(s):  
Ya.V. Rybalka
Keyword(s):  
Wound Healing ◽  
Chronic Wounds ◽  
Platelet Rich Plasma ◽  
Control Group ◽  
Wound Area ◽  
Area Reduction ◽  
Autologous Platelet Rich Plasma ◽  
Group 2 ◽  
Autologous Platelet ◽  
Group 1

Introduction. The analysis of the effectiveness of applying autologous platelet-rich plasma was performed in order to improve the results of surgical treatment of patients with wounds that heal slowly. Materials. The study is based on the analysis of the treatment of 61 patients who suffered from slow-healing wounds of venous genesis with a diameter of no more than 8 cm on the lower extremities and took the treatment at the surgical ward. The proposed method was based on obtaining autologous enriched platelet plasma followed by its injection along the periphery of the wound. The degree of reduction of the wound surface area was assessed by the method of contact planimetry per day. The patients of the 1st group demonstrated the wound area reduction by 5.1 ± 0.6%, and the patients of the group 2 – by 1.8 ± 0.4% (p <0005). The results indicate an increase in the rate of wound healing in patients in group 1 compared with patients in group 2. During the entire follow-up period (12 weeks), complete wound epithelialisation occurred in 21 (70%) patients in group 1 and in 10 (33.3%) patients of the group 2. The wound area decreased by more than 50% in 6 patients of group 1 (20%) and in 2 (7%) patients of the control group. The wound area decreased less than by 50% in 3 patients in group 1 (10%), and in 19 patients in group 2 (60%). The use of PRP therapy has been proven to have a significant positive effect on the process of wound healing compared with the use of standard methods used for the control group. Conclusion: increase in the wound healing pace in patients of group 1 (test) compared with patients of group 2 (control) can be explained by using autologous platelet-enriched plasma as an additional means to the standard therapy.

Wound Healing in Beagle Dogs after Palatal Repair without Denudation of Bone

1995 ◽  
Vol 32 (5) ◽  
pp. 363-370 ◽  
Author(s):  
Thomas S. Leenstra ◽  
Jaap C. Maltha ◽  
Anne Marie Kuijpers-Jagtman ◽  
Paul H.M. Spauwen
Keyword(s):  
Wound Healing ◽  
Scar Tissue ◽  
Control Group ◽  
Beagle Dogs ◽  
Flap Technique ◽  
Group 2 ◽  
Palatal Bone ◽  
Lamellar Type ◽  
Palatal Surgery ◽  
Group 1

The connection of scar tissue to palatal bone by Sharpey's fibers, after cleft palate surgery, might lead to maxillary growth inhibition. The aim of this study, in beagle dogs, was to investigate the possibility of preventing the development of Sharpey's fibers by means of a modified surgical technique. In group 1, palatal repair according to von Langenbeck, was simulated. In group 2, palatal surgery was performed using a new partially split flap technique. The palates were histologically evaluated 12 weeks after surgery and compared with a control group. In group 1, the scar tissue was firmly attached to bundle bone by means of Sharpey's fibers. In group 2 and in the control group, this kind of attachment was not found; the bone was of the lamellar type. The partially split flap technique had led to the development of vaguely demarcated scar tissue and it had prevented, to a large extent, development of Sharpey's fibers.

Hearing and Exposure to Music in Adolescents From Four Schools of Córdoba, Argentina

2021 ◽  
pp. 1-14
Author(s):  
Sara Gaetán ◽  
Jimena Muratore ◽  
Ana Luz Maggi ◽  
Jorge Pérez Villalobo ◽  
María de los Ángeles Hinalaf
Keyword(s):  
Otoacoustic Emissions ◽  
Noise Exposure ◽  
Hearing Tests ◽  
Cross Sectional ◽  
High Exposure ◽  
Distortion Product ◽  
Hearing Thresholds ◽  
Low Exposure ◽  
Group 2 ◽  
Group 1

Purposes The aims of this study are (a) to characterize the hearing of adolescents from four schools of Córdoba, Argentina, through the analysis of conventional and extended high-frequency audiometric thresholds and otoacoustic emissions (OAEs) and (b) to analyze the association between the mentioned hearing tests and exposure to music. Method It was a cross-sectional correlational descriptive study. Hearing thresholds (250–16000 Hz), transient evoked OAEs, and distortion product OAEs were evaluated in 225 adolescents (450 ears) aged 14 and 15 years. The ears were split into two groups: Group 1 had thresholds ≤ 21 dB HL in all frequencies, and Group 2 had thresholds > 21 dB HL in at least one. Exposure to music was evaluated through a questionnaire. Results Statistically significant differences were found in both ears between Groups 1 and 2. A notch at 3000–6000 Hz was noted in both groups. Group 2 showed a progressive threshold increase from 9000 Hz. Amplitude decrease, negative values, absent distortion product OAEs, and transient evoked OAEs were noted even in Group 1. A statistically significant association between Groups 1 and 2 and the presence/absence of OAEs was observed in most frequencies. Ears with moderate or high exposure to music had greater hearing thresholds compared to ears with low exposure at most frequencies; this was more evident in Group 1. Regarding exposure to music and OAEs, no significant differences were found between the exposure categories. Conclusions The findings highlight the value of implementing hearing conservation programs in Argentina, analyzing hearing tests correlated with questionnaires about recreational noise exposure in order to detect vulnerable ears early.

Topical N-acetylcysteine Reduces Interleukin-1-alpha in Tear Fluid after Laser Subepithelial Keratectomy

2009 ◽  
Vol 19 (4) ◽  
pp. 554-559 ◽  
Author(s):  
Berrak Urgancioglu ◽  
Kamil Bilgihan ◽  
Doruk Engin ◽  
Meltem Yalinay Cirak ◽  
Ahmet Hondur ◽  
...  
Keyword(s):  
Wound Healing ◽  
Interleukin 1 ◽  
Early Postoperative Period ◽  
Tear Fluid ◽  
Control Group ◽  
Corneal Wound Healing ◽  
Corneal Wound ◽  
The Mean ◽  
Group 2 ◽  
Group 1

Purpose To evaluate the effect of topical N-acetylcysteine (NAC) on interleukin 1-alpha (IL-1α) levels in tear fluid after myopic laser subepithelial keratectomy (LASEK) and its possible role in modulating corneal wound healing. Methods Twenty-six eyes of 13 patients who underwent myopic LASEK were divided into 2 groups. Group 1 (n=10 eyes) was used as a control group. All patients received topical lomefloxacin and dexamethasone postoperatively. Additionally, patients in Group 2 received topical NAC for 1 month postoperatively. Tear fluid samples were collected with microcapillary tubes preoperatively, on the first and on the fifth postoperative day, and the release of IL-1α in tear fluid was calculated. Haze grading and confocal microscopic examination were performed at 1 and 3 months postoperatively. Results The mean IL-1-α release values were 0.285±0.159 pg/min in Group 1 and 0.235±0.142 pg/min in Group 2 preoperatively. In Group 1, the values were 0.243±0.155 pg/min on day 1 and 0.164±0.125 pg/min on day 5. In Group 2, the mean IL-1α release values were 0.220±0.200 pg/min on day 1 and 0.080±0.079 pg/min on day 5. The difference between the groups was significant only for day 5 (p<0.05). Mean corneal haze score and grey scale value in confocal microscopy were significantly higher (p<0.05) in Group 1 at 1 month. However, at 3 months there was no difference between groups (p>0.05). Conclusions NAC seems to have an additive effect to steroids in suppressing IL-1α levels in tear fluid and may be clinically advantageous in modulating corneal wound healing during the early postoperative period after LASEK.

scholarly journals Prophylactic Effect of Ethanolic Extract of Saussurea Costus Roots Against Hepato- Renal Toxicity Induced by Diazinon in Chickens

2020 ◽  
Vol 13 (2) ◽  
pp. 34-51
Author(s):  
Alaa K. Hamad ◽  
Mariam Kadhem
Keyword(s):  
Morphological Changes ◽  
Clinical Signs ◽  
Ethanolic Extract ◽  
Inflammatory Cells ◽  
The Body ◽  
Control Group ◽  
Central Vein ◽  
Histopathological Changes ◽  
Group 2 ◽  
Group 1

The study was performed to investigate the prophylactic role of Saussurea costus roots in the functional and histological changes caused by diazinon in the liver and kidneysin chickens. 18 chickens were used of Arbor Acres strain at 54 days of age and 1300-1500 gm average of body weight was used for this purpose. The birds were divided into 3 groups equally (6chicken in each groups), Group 1: served as a control negative received distilled water 1 ml. Group 2: served as a positive control group, received diazinon at a dose of 0.2 mg/kg/day. Group 3: were given an ethanolic extractof Saussurea costus at dose 300 mg/kg, then after one hour administrated diazinon at the dose of 0.2 mg/kg/day, this group served as a prophylactic group. The doses were given orally once daily for 4 weeks.The results of group 2 showed clinical signs such as ruffled feathers, salivation, diarrhea, breathing from the mouth, teary eyes, drooping of wings. The body weights of the chickens and weights of the liver and kidneys of group 2 significantly declined (p≤ 0.05) compared with groups 1 and 3. There was a significant decrease (p≤ 0.05) in WBC count, lymphocyte, total protein, albumin, GSH, SOD, CAT, and GPX levels, while a significant increase (p≤0.05) in heterophil, ALT, AST, creatinine, urea, uric acid, and MDA compared with group 1. The gross examination of the liver and kidney of group 2 were pale, easily crumbles and smaller than that of group 1. Histopathological changes of the liver of group 2 including congested and dilated central vein, vacuolated cytoplasm of hepatocytes, focal necrotic tissue filled with inflammatory cells, thickening of the bile duct, thickening wall of the portal artery. fibroblast in portal area, dilated sinusoid. Histopathological changes of the kidney including dilatation of renal tubule, hemorrhage, and atrophy in the glomerulus. we concluded that administration of ethanolic extractof Saussurea costus resulted in amelioration of the morphological changes in diazinon treated chickens, improved parameters and restored the parameters to near normal compared with group 1. These results revealed that Saussurea costus roots acts as an antioxidant substance and has a hepatic and renoprotective effect against toxicity induced by diazinon.

scholarly journals The Effects of Curcumin on Wound Healing in a Rat Model of Nasal Mucosal Trauma

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Gokhan Emiroglu ◽  
Zerrin Ozergin Coskun ◽  
Yildiray Kalkan ◽  
Ozlem Celebi Erdivanli ◽  
Levent Tumkaya ◽  
...  
Keyword(s):  
Wound Healing ◽  
Dimethyl Sulfoxide ◽  
Control Group ◽  
Albino Rats ◽  
Sprague Dawley ◽  
Group 4 ◽  
Leukocytic Infiltration ◽  
Sham Control Group ◽  
Group 2 ◽  
Group 1

We explored the effects of topical curcumin on the healing of nasal mucosal wounds. A total of 32 Sprague-Dawley Albino rats were randomized in equal numbers into four groups, and unilateral nasal wounds were created using an interdental brush. Group 1 (the sham-control group) contained untreated rats with traumatized right-side nasal cavities; Group 2 and 3 rats were similarly traumatized and treated with topical curcumin (5 and 10 mg/mL) dissolved in dimethyl sulfoxide daily for 7 days after trauma; Group 4 rats were treated with topical dimethyl sulfoxide only. All rats were decapitated on day 15 and the healing sites evaluated by blinded observers in terms of the presence of cellular hyperplasia, goblet cell hypertrophy and degeneration, leucocytic infiltration, ciliary loss and degeneration, edema, and vascular dilation. On histopathological evaluation, all of cellular hyperplasia, leukocytic infiltration, and edema were significantly reduced in Group 3 compared with Group 1 (p=0.001, p=0.004, and p=0.008, resp.). Thus, curcumin reduced the inflammatory response and significantly accelerated wound healing.

scholarly journals Experimental mouse model of bronchial asthma induced by house dust mite Dermatophagoides pteronyssinus allergenic extract

2015 ◽  
Vol 12 (6) ◽  
pp. 25-33
Author(s):  
A A Babakhin ◽  
A A Laskin ◽  
O Yu Kamishnikov ◽  
N N Shershakova ◽  
I P Shilovskiy ◽  
...  
Keyword(s):  
Allergic Inflammation ◽  
Inflammatory Cells ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Dust Mite ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

The purpose of this study was to develop a mouse model of asthma (MMA) using house dust mite Dermatophagoides pteronyssinus (Der p) extract. Methods. BALB/c mice were i.p. immunized with different doses of Der p lyophilized extract three times in three week interval in the mixture with Al(OH)3. 8 weeks after the final immunization mice were challenged with Der p during five consecutive days by intranasal applications (INA) or aerosol administration (AA). All mice were divided into 5 experimental groups: group 1 was immunized with 50 ^g/mouse of Der p (in protein equivalent) in the mixture with 2 mg/mouse Al(OH)3 and challenged by INA; group 2 was immunized in the same way and challenged by AA; group 3 was immunized with 100 ^g/mouse Der p in the mixture with 2 mg/mouse of Al(OH)3 and challenged by INA; group was immunized in the same way and challenged by AA; group 5 (negative control) was immunized and challenged with saline. 24 hours after the last challenge airway hyperresponsiveness (AHR) to different concentrations of metha-choline was evaluated in all groups by whole-body plethysmography. 48 hours after the last challenge in all groups blood was collected for differential cell count, brochoalveolar lavage fluid (BALF) was sampled for the determination of inflammatory cells and lungs were removed for histological analysis. Histopathological changes in lungs (allergic inflammation) were graded according to semi-quantitative scoring system. Anti-Der p IgE, IgG1 and IgG2a antibodies in individual sera samples were detected by ELISA seven days after the last immunization and 48 hours after the challenge. Results. The levels of anti-Der p IgE antibodies in groups 1-4 before as well as after the challenge were substantially higher than that of in the group 5 (negative control). The highest level of serum Der p-specific IgE antibodies was observed in the group 2. The levels of anti-Der p IgG1 and IgG2a antibodies in the groups 1-4 during all periods of observation were higher than that of group 5 (negative control). At the same time the maximal levels of anti-Der p IgG1 and IgG2a antibodies were observed in group 3 both after immunization and after challenge. The maximum of AHR was observed in the groups 1 and 3 challenged by INA. Analysis of cell composition in BALF demonstrated significant elevated number of eosinophils in group 3 in comparison with group 5 (negative control) and other experimental groups. Regarding peripheral blood leukocyte count we observed decreasing of band neutrophils in group 4 and increasing of segmented neutrophils in groups 1 and 3 in compare to group 5 (negative control). In group 1 we found statistically significant decreasing of lymphocytes and increasing of eosinophils in compare to negative control group 5. Histological picture of general allergic inflammation in lungs as well as peribronchial and perivascular infiltration with inflammatory cells were the most noticeable (according to score system) in group 3 in comparison with negative control group and other experimental groups. Conclusion. Data obtained indicate that immunization (sensitization) of mice with Der p in a dose 100 μg/mouse together with Al(OH)3 and challenge with Der p by mean of intranasal applications is a suitable approach for modeling of mouse allergic asthma.

scholarly journals Otoacoustic Emissions in Children with Long-Term Middle Ear Disease

Life ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 287
Author(s):  
Milaine Dominici Sanfins ◽  
Luisa Frata Bertazolli ◽  
Piotr H. Skarzynski ◽  
Magdalena Beata Skarzynska ◽  
Caroline Donadon ◽  
...  
Keyword(s):  
Middle Ear ◽  
Otoacoustic Emissions ◽  
Signal To Noise Ratio ◽  
Bone Conduction ◽  
Otoacoustic Emission ◽  
Control Group ◽  
Distortion Product ◽  
Middle Ear Infection ◽  
Ventilation Tubes ◽  
History Of

Introduction: Otoacoustic emissions (OAEs) evaluate the functional status of the cochlea. Repeated otitis media (OM) can cause changes in the peripheral structures of the auditory system, and, in this way, middle ear infection may irreversibly damage the middle ear, or even the cochlea. Objectives: To analyze the results of transiently evoked otoacoustic emissions (TEOAEs) and distortion product otoacoustic emissions (DPOAEs) in individuals with a history of OM. Method: Participants with 8 to 16 years of schooling were split into two groups: a control group (CG) of 50 subjects who had no history of otological disease and an experimental group (EG) of 50 subjects who had a history of recurrent otitis in childhood and had consequently undergone myringotomy to insert bilateral ventilation tubes. All children underwent basic audiological assessment (tonal audiometry, speech audiometry, and immittance testing) and otoacoustic emission testing (TEOAEs and DPOAEs). Results: There were no significant differences between the groups when audiometrically tested via air and bone conduction. OAEs were found in all CG subjects. For the EG, there were no TEOAE responses in 17 ears and no DPOAEs in nine ears; response amplitudes were lower at all frequencies. The emission level and the signal-to-noise ratio were statistically different between the two groups, and OAEs in the EG were statistically smaller compared to the GC. Conclusion: In the EG, responses were more likely to be absent and were of statistically smaller amplitude compared to the CG. A history of repeated OM apparently interferes with the generation and transmission of TEOAEs and DPOAEs.

scholarly journals Effects of GSM-like radiofrequency on distortion product otoacoustic emissions in pregnant adult rabbits

2009 ◽  
Vol 32 (2) ◽  
pp. 112 ◽  
Author(s):  
Gürer G Budak ◽  
Nuray Bayar Muluk ◽  
Göknur Güler Öztürk ◽  
Bilgehan Budak ◽  
Alpaslan Apan ◽  
...  
Keyword(s):  
Otoacoustic Emissions ◽  
Prolonged Exposure ◽  
Distortion Product Otoacoustic Emissions ◽  
Distortion Product ◽  
Group 4 ◽  
New Zealand White Rabbits ◽  
Group 3 ◽  
Group 2 ◽  
Harmful Effects ◽  
Group 1

Objectives: To determine the effects of 1800 MHz GSM-like Radiofrequency (RFR) on the cochlear functions of pregnant adult rabbits by Distortion Product Otoacoustic Emissions (DPOAEs). Methods: Eighteen 13-month-old pregnant and eighteen 13-month-old non-pregnant New Zealand White rabbits were studied. They were randomly divided into four groups. Nine pregnant rabbits (Group 2) and nine non-pregnant rabbits (Group 4) were exposed to 1800 MHz GSM-like RFR 15 min daily for 7 days. Nine pregnant (Group 1) and nine non-pregnant rabbits (Group 3) were not exposed to GSM like RFR. Cochlear functions were assessed by DPOAEs at 1.0-8.0 kHz. Results: In all pregnant groups except 2.0 kHz, DPOAE amplitudes were not different in Group 2 and Group1. In Group 4, DPOAE amplitudes at 1.0-4.0 kHz (-1.68 dB SPL at 1.0 kHz, 3.05 dB SPL at 1.5 kHz, 2.96 dB SPL at 2.0 kHz, 1.30 dB SPL at 3.0 kHz and 12.22 dB SPL at 4.0 kHz) were lower than Group 3 (8.67 dB SPL at 1.0 kHz, 17.67 dB SPL at 1.5 kHz, 26.10 dB SPL at 2.0 kHz, 18.10 dB SPL at 3.0 kHz and 35.13 dB SPL at 4.0 kHz) (P < 0.0125). In the pregnant group, harmful effects of GSM-like RFR were less than in the non-pregnant group. Conclusion: GSM-like RFR caused decreases in DPOAE amplitudes mainly in non-pregnant adult rabbits. Prolonged exposure may affect the DPOAE amplitude. Recommendations are given to prevent the potential hazardous effects of RF in humans.

Sign in / Sign up

Export Citation Format

Share Document