Albumin concentration determined by the modified bromocresol purple method is superior to that by the bromocresol green method for assessing nutritional status in malnourished patients with inflammation

Study Design: Cross-sectional study. Objectives: Thus, this study aimed to assess the epidemiological profile of a patient sample that underwent spinal surgery regarding their nutritional and vitamin D status. Methods: Serum albumin and vitamin D (25-hydroxyvitamin D) levels were measured in patients with different spinal surgical approaches and various pathologies at a single institution. 112 patients were retrospectively identified for inclusion and stratified by age into 4 age groups and by pathology. The nutritional status of the patients was classified in vitamin D inadequacy (< 30ng/mL), vitamin D deficiency (<20ng/mL), and hypoalbuminemia (<3.5g/dL). Data was analyzed comparing vitamin D, and albumin means considering gender, age group, and pathologies. Results: Twenty-eight (25.2%) patients had hypoalbuminemia. There was no difference between gender (p = 0.988); there was a significant decrease in albumin concentration increasing the age (p < 0.001). The prevalence of hypoalbuminemia was significantly higher in patients with trauma, tumor and infection than in those patients with degenerative and deformity diseases (p = 0.003). The prevalence of vitamin D inadequacy was 33.7%, and that of deficiency was 62.2%, while severe deficiency (< 10 ng/mL) in 16.3%. The vitamin D concentration was significantly different among the pathologies (P = 0.047), the lower concentration occurring in patients with tumor. Conclusion: Older patients, as well as patients with tumor and infectious pathologies, seem to have a higher prevalence of hypoalbuminemia, inferring malnutrition. There was a low epidemic level of vitamin D concentration, almost all patients presenting some degree of hypovitaminosis D, independent of age, gender and nutritional status.

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O21 Is hypoalbuminaemia associated with amputation-free survival in chronic limb-threatening ischaemia? A retrospective cohort study

British Journal of Surgery ◽

10.1093/bjs/znab282.026 ◽

2021 ◽

Vol 108 (Supplement_5) ◽

Author(s):

A T O Nickinson ◽

I Black ◽

J S M Houghton ◽

R D Sayers

Keyword(s):

Nutritional Status ◽

Inflammatory Markers ◽

White Cell Count ◽

Tissue Loss ◽

Albumin Concentration ◽

Free Survival ◽

Cardiovascular Comorbidities ◽

Operative Outcomes ◽

One Year ◽

The Relationship

Abstract Introduction Serum albumin is a recognised marker of nutritional status. Whilst associated with post-operative outcomes, the relationship with amputation-related outcomes in chronic limb-threatening ischaemia (CLTI) remains unclear. It is also uncertain whether any relationship may be confounded by systematic inflammation. We aimed to investigate whether hypoalbuminaemia is associated with amputation-related outcomes and evaluate its relationship with other inflammatory markers. Method A retrospective study of patients managed for CLTI at a vascular centre between 01/01/2013–31/12/2015 was undertaken. Patients were identified from local coding databases. Patient demographics and comorbidities, results of admission bloods and procedural data were recorded from electronic records. Hypoalbuminaemia was defined as albumin concentration <35 g/L. One-year amputation-free survival (AFS) was the primary outcome. Cox’s proportional hazard models were calculated to compare the risk of amputation/death. Linear regression was performed to investigate the relationship between albumin and other inflammatory markers. Result 374 patients were included (mean albumin concentration = 38.8g/L [SD = 5.2]), with 72 patients being hypoalbuminaemic. No differences were observed in sex (P=0.678), age (P=0.207), history of tissue loss (P=0.057), or cardiovascular comorbidities between groups, however a greater proportion of patients with hypoalbuminaemia had COPD (P=0.002). Patients with hypoalbuminaemia had a significantly higher combined risk of 1-year amputation/death compared to those with normal albumin concentration (HR 1.94, 95% CI 1.29–2.91). White cell count and CRP concentration were significant predictors of albumin concentration (r2=0.113, P<0.001). Conclusion Hypoalbuminaemia is an important prognostic biomarker of AFS in CLTI, however this association may relate to a complex relationship between nutritional status and inflammation. Take-home Message Albumin is more than just a marker of nutritional status in patients with chronic limb-threatening ischaemia.

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Association between kidney function, nutritional status and anthropometric measures in older people

BMC Geriatrics ◽

10.1186/s12877-020-01699-1 ◽

2020 ◽

Vol 20 (S1) ◽

Cited By ~ 1

Author(s):

Agnieszka Guligowska ◽

◽

Andrea Corsonello ◽

Małgorzata Pigłowska ◽

Regina Roller-Wirnsberger ◽

...

Keyword(s):

Nutritional Status ◽

Serum Albumin ◽

Nutritional Assessment ◽

Short Form ◽

Mini Nutritional Assessment ◽

Overweight And Obesity ◽

Community Dwelling ◽

Albumin Concentration ◽

Anthropometric Measures ◽

Baseline Phase

Abstract Background Different mechanisms connect the nutritional status with the occurrence and the course of chronic kidney disease (CKD). The end-stage renal disease is complicated by catabolic inflammatory reactions and cachexia which leads to malnutrition (undernutrition). On the other hand, obesity is an important risk factor for the development and acceleration of CKD. Methods In the SCOPE study, community-dwelling persons aged 75 years and over, from 6 European countries and Israel were examined at the baseline phase. We assessed the relationship between anthropometric measures (Body Mass Index (BMI), circumferences of arm (AC), waist (WC), hip (HC), and calf (CC), waist-to-hip ratio - WHR, waist-to-height ratio - WHtR, risk of malnutrition (Mini Nutritional Assessment - MNA), serum albumin) and estimated glomerular filtration rate (eGFR) calculated by Berlin Initiative Study (BIS) equation. Results We studied 2151 subjects (932 men and 1219 women) with a mean age of 79.5 ± 5.9 years. A total of 1333 (62%) participants had CKD (GRF < 60 ml/min/1.73 m2). Negative correlations between eGFR and weight, AC, WC, HC, CC, BMI, WHtR were observed. Positive correlation occurred between eGFR and MNA score (Spearman’s rho = 0.11) and albumin concentration (rho = 0.09). Higher weight, AC, WC, HC, CC, BMI and WHtR increased the odds ratio of CKD; higher MNA (OR = 0.98, 95% CI 0.94–1.0) and higher serum albumin (OR = 0.73, 95% CI 0.53–1.0) were weakly associated with reduced odds. The risk of malnutrition was the highest with eGFR < 30 as compared to eGFR > 60 (OR = 2.95, 95%CI = 1.77–4.94 for MNA < 24; OR = 5.54, 95%CI = 1.66–18.5 for hypoalbuminemia < 3.5 g/dL). Conclusion The population of community dwelling people aged 75+ with CKD shows general features of overweight and obesity with a small prevalence of malnutrition. For anthropometric measures, the strongest association with eGFR and the highest odds of CKD were identified using WC, HC, CC and WHtR. Albumin level and MNA, but not MNA Short Form, indicated an increased odds of malnutrition with a decrease in eGFR.

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Spectrophotometric Determination of Gemifloxacin Mesylate, Moxifloxacin Hydrochloride, and Enrofloxacin in Pharmaceutical Formulations Using Acid Dyes

Journal of Analytical Methods in Chemistry ◽

10.1155/2014/286379 ◽

2014 ◽

Vol 2014 ◽

pp. 1-16 ◽

Cited By ~ 6

Author(s):

Ayman A. Gouda ◽

Alaa S. Amin ◽

Ragaa El-Sheikh ◽

Amira G. Yousef

Keyword(s):

Pharmaceutical Formulations ◽

Bromothymol Blue ◽

Bromocresol Green ◽

Bromophenol Blue ◽

Bromocresol Purple ◽

Acid Dyes ◽

Spectrophotometric Methods ◽

Significant Difference ◽

Comparison Of The Results

Simple, rapid, and extractive spectrophotometric methods were developed for the determination of some fluoroquinolones antibiotics: gemifloxacin mesylate (GMF), moxifloxacin hydrochloride (MXF), and enrofloxacin (ENF) in pure forms and pharmaceutical formulations. These methods are based on the formation of ion-pair complexes between the basic drugs and acid dyes, namely, bromocresol green (BCG), bromocresol purple (BCP), bromophenol blue (BPB), bromothymol blue (BTB), and methyl orange (MO) in acidic buffer solutions. The formed complexes were extracted with chloroform and measured at 420, 408, 416, 415, and 422 nm for BCG, BCP, BPB, BTB, and MO, respectively, for GMF; at 410, 415, 416, and 420 nm for BCP, BTB, BPB, and MO, respectively, for MXF; and at 419 and 414 nm for BCG and BTB, respectively, in case of ENF. The analytical parameters and their effects are investigated. Beer’s law was obeyed in the ranges 1.0–30, 1.0–20, and 2.0–24 μg mL−1for GMF, MXF, and ENF, respectively. The proposed methods have been applied successfully for the analysis of the studied drugs in pure forms and pharmaceutical formulations. Statistical comparison of the results with the reference methods showed excellent agreement and indicated no significant difference in accuracy and precision.

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Binding of bromocresol green and bromocresol purple to albumin in hemodialysis patients

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2017-0444 ◽

2018 ◽

Vol 56 (3) ◽

pp. 436-440 ◽

Cited By ~ 5

Author(s):

Sigurd Delanghe ◽

Wim Van Biesen ◽

Nadeige Van de Velde ◽

Sunny Eloot ◽

Anneleen Pletinck ◽

...

Keyword(s):

Serum Albumin ◽

Acute Phase ◽

High Performance ◽

Acute Phase Proteins ◽

Hemodialysis Patients ◽

Uremic Toxins ◽

Bromocresol Green ◽

Bromocresol Purple ◽

Acid Glycoprotein ◽

Reactive Protein

AbstractBackground:Colorimetric albumin assays based on binding to bromocresol purple (BCP) and bromocresol green (BCG) yield different results in chronic kidney disease. Altered dye binding of carbamylated albumin has been suggested as a cause. In the present study, a detailed analysis was carried out in which uremic toxins, acute phase proteins and Kt/V, a parameter describing hemodialysis efficiency, were compared with colorimetrically assayed (BCP and BCG) serum albumin.Methods:Albumin was assayed using immunonephelometry on a BN II nephelometer and colorimetrically based on, respectively, BCP and BCG on a Modular P analyzer. Uremic toxins were assessed using high-performance liquid chromatography. Acute phase proteins (C-reactive protein and α1-acid glycoprotein) and plasma protein α2-macroglobulin were assayed nephelometrically. In parallel, Kt/V was calculated.Results:Sixty-two serum specimens originating from hemodialysis patients were analyzed. Among the uremic toxins investigated, total para-cresyl sulfate (PCS) showed a significant positive correlation with the BCP/BCG ratio. The serum α1-acid glycoprotein concentration correlated negatively with the BCP/BCG ratio. The BCP/BCG ratio showed also a negative correlation with Kt/V.Conclusions:In renal insufficiency, the BCP/BCG ratio of serum albumin is affected by multiple factors: next to carbamylation, uremic toxins (total PCS) and α1-acid glycoprotein also play a role.

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Biosorption of Bromo-based Dyes from Wastewater Using Low-Cost Adsorbents: A Review

Journal of Scientific Research and Reports ◽

10.9734/jsrr/2020/v26i830294 ◽

2020 ◽

pp. 34-56

Author(s):

Ebenezer Olujimi Dada ◽

Ilesanmi Ademola Ojo ◽

Abass Olanrewaju Alade ◽

Tinuade Jolaade Afolabi ◽

Monsurat Omolola Jimoh ◽

...

Keyword(s):

Effective Treatment ◽

Adsorption Process ◽

Low Cost ◽

Agricultural Waste ◽

Bromothymol Blue ◽

Bromocresol Green ◽

Bromophenol Blue ◽

Bromocresol Purple ◽

Aquatic Communities ◽

Triarylmethane Dyes

The dyes in the effluents discharged into water bodies, aimlessly, are displeasing aesthetically and pose hazards to aquatic communities. The use of adsorption process has been adopted for effective treatment of wastewater containing dyes. The removal of Bromophenol blue (BPB), Bromocresol green (BCG), Bromocresol purple (BCP), and Bromothymol blue (BTB) dyes (a family of triarylmethane dyes) through adsorption process using several cheaply available non-conventional agricultural-waste based adsorbents was reviewed in this report. The gaps in the treatment trend further indicate the prospect of adapting various lignocellulose and other biogenic materials for the removal of Bromo-based dyes from wastewater.

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Laboratory Performance in Albumin and Total Protein Measurement Using a Commutable Specimen: Results of a College of American Pathologists Study

Archives of Pathology & Laboratory Medicine ◽

10.5858/arpa.2012-0152-cp ◽

2013 ◽

Vol 137 (7) ◽

pp. 912-920 ◽

Cited By ~ 9

Author(s):

Stanley F. Lo ◽

W. Greg Miller ◽

Basil T. Doumas

Keyword(s):

Serum Sample ◽

Total Protein ◽

Total Error ◽

Peer Groups ◽

Bromocresol Green ◽

Bromocresol Purple ◽

Error Performance ◽

Fresh Frozen ◽

Total Protein Measurement ◽

Protein Measurement

Context.—Discrepant results for serum constituents were observed among peer groups in the College of American Pathologists Comprehensive Chemistry Survey. Objectives.—To assess the performance of serum albumin and total protein measurement procedures and to evaluate the commutability of the conventional survey specimens. Design.—A fresh frozen, off-the-clot serum sample was included along with 4 conventional survey specimens. The fresh frozen, off-the-clot serum sample was prepared in a manner expected to confer commutability with native clinical samples. Results.—For the fresh frozen, off-the-clot serum sample, the mean values for 17 peer-groups were −0.07 to 0.32 g/dL from the bromocresol green albumin designated comparison method, whereas 4 VITROS (Ortho Clinical Diagnostics, Rochester, New York) peer groups differed by −0.29 to −0.37 g/dL (15 of 21 differences [71%] had P < .001). For bromocresol purple albumin methods, the mean differences from the designated comparison method from 8 peer groups were 0.25 to 0.47 g/dL (all had P < .001). For total protein methods, 23 peer group mean values were −0.07 to 0.15 g/dL from the reference measurement procedure (12 of 24 [50%] had P < .001). The Beckman (Fullerton, California) Synchron LX20 had a bias of −0.30 g/dL (P <.001). The commutability of the conventional specimens was acceptable for 23 of 24 bromocresol green method-material combinations (96%) and 13 of 16 bromocresol purple albumin method-material combinations (81%). All (100%) of the 36 method-material combinations had acceptable commutability for total protein. Conclusions.—One (2.2%) of the instrument systems (Synchron) using bromocresol green and none (0%) of the instrument systems using bromocresol purple had satisfactory total-error performance for albumin measurement. Differences in results between bromocresol green and bromocresol purple methods precluded using common reference intervals for interpreting results for serum albumin. Eight of 9 instrument systems (86.5%) had satisfactory total-error performance for total protein measurement.

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Prognostic nutritional index as indicator of immune nutritional status of patients with COVID-19

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000730 ◽

2021 ◽

Author(s):

Iskender Ekinci ◽

Hafize Uzun ◽

Irem Kirac Utku ◽

Hanise Ozkan ◽

Mitat Buyukkaba ◽

...

Keyword(s):

Nutritional Status ◽

Oxygen Saturation ◽

White Blood Cells ◽

Prognostic Nutritional Index ◽

Multivariate Regression Analysis ◽

Albumin Concentration ◽

Reactive Protein ◽

Nutritional Index ◽

Failure Assessment ◽

Group 2

Abstract. Purpose: This study aimed to investigate the effect of the nutritional status, as assessed by the prognostic nutritional index (PNI) on the disease prognosis of patients with COVID-19. Methods: This retrospective study included 282 patients with COVID-19. The PNI score of all patients, 147 of whom were male, with a mean age of 56.4±15.3 years, was calculated. According to the PNI score, the patients with normal and mild malnutrition constituted group-1 (n=159) and the patients with moderate-to-severe and serious malnutrition constituted group-2 (n=123). Results: The PNI score was correlated with age (r=−0.146, p=0.014); oxygen saturation (r=0.190, p=0.001); heart rate (r=−0.117, p=0.05); hospitalization duration (r=−0.266, p<0.001); white blood cells (r=0.156, p=0.009); hemoglobin (r=0.307, p<0.001); C-reactive protein (CRP) (r=−0.346, p<0.001); creatinine (r=−0.184, p=0.002); D-dimer (r=−0.304, p<0.001); ferritin (r=−0.283, p<0.001); procalcitonin (r=−0.287, p<0.001); the confusion, urea, respiratory rate, blood pressure, and age ≥65 years score (r=−0.217, p<0.001); and the quick sequential organ failure assessment score (r=−0.261, p<0.001) in patients with COVID-19. Mortality was significantly higher in Group 2 (p<0.001). Survival was significantly higher if PNI score was >41.2 (p<0.001, sensitivity: 78.7% and specificity: 84.2%). In multivariate regression analysis, among various other parameters, only PNI score and oxygen saturation had a significant effect on the disease course (p=0.02 and p=0.045, respectively). Conclusion: PNI, calculated from the serum albumin concentration and total lymphocyte count, is a simple and objective indicator that assesses the immune nutritional status of patients with COVID-19. The presence of malnutrition has a high predictive value in predicting the severity of COVID-19. Our data suggest that the PNI might be useful for risk stratification of patients with COVID-19 in clinical practice.

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