Emergence of Beta -lactamase-prod uci n g Aerobic and Anaerobic Bacteria in the Oropharynx of Children Following Penicillin Chemotherapy

1984 ◽  
Vol 23 (6) ◽  
pp. 338-341 ◽  
Author(s):  
Itzhak Brook ◽  
Alan E. Gober
Keyword(s):  
Anaerobic Bacteria ◽  
Beta Lactamase ◽  
Aerobic And Anaerobic

Aerobic and Anaerobic Bacteria in Tonsils of Children with Recurrent Tonsillitis

1981 ◽  
Vol 90 (3) ◽  
pp. 261-263 ◽  
Author(s):  
Itzhak Brook ◽  
Paula Yocum ◽  
Ellen M. Friedman
Keyword(s):  
Anaerobic Bacteria ◽  
Core Material ◽  
Beta Lactamase ◽  
Type B ◽  
Recurrent Tonsillitis ◽  
Patients Âgés ◽  
Anaerobic Microorganisms ◽  
Group A ◽  
Group B ◽  
Aerobic And Anaerobic

Tonsils were obtained from 50 children suffering from recurrent tonsillitis. Patients' ages ranged from 2.5 to 17 years (mean 6 years); 29 were males and 21 females. The tonsils were sectioned in half after heat searing of the surface and the core material was cultured for aerobic and anaerobic microorganisms. Mixed aerobic and anaerobic flora was obtained in all patients, yielding an average of 7.8 isolates (4.1 anaerobes and 3.7 aerobes) per specimen. There were 207 anaerobes isolated. The predominant isolates were 101 Bacteroides sp (including 10 B fragilis group, and 47 B melaninogenicus group), 39 Fusobacterium sp, 34 Gram-positive anaerobic cocci (25 Peptococcus sp and 9 Peptostreptococcus sp) and 16 Veillonella sp. There were 185 aerobic isolates. The predominant isolates were 41 α-hemolytic streptococci, 24 Staphylococcus aureus, 19 β-hemolytic streptococci (11 group A, 4 group B, and 2 each group C and F), 14 Haemophilus sp (including 12 H influenzae type B) and 5 H parainfluenzae. Beta-lactamase production was noted in 56 isolates recovered from 37 tonsils. These were all isolates of S aureus (24) and B fragilis (10), 15 of 47 B melaninogenicus (32%), 5 of 12 B oralis (42%), and 2 of 12 H influenzae type B (17%). Our findings indicate the polymicrobial aerobic and anaerobic nature of deep tonsillar flora in children with recurrent tonsillitis, and demonstrate the presence of many β-lactamase-producing organisms in 74% of the patients.

Aerobic and Anaerobic Bacteria in the Middle ear and ear Canal in Acute Otitis Media

1982 ◽  
Vol 93 (sup386) ◽  
pp. 100-102 ◽  
Author(s):  
J. Luotonen ◽  
A. M. M. Jokipii ◽  
P. Sipilä ◽  
J. Väyrynen ◽  
L. Jokipii ◽  
...  
Keyword(s):  
Otitis Media ◽  
Middle Ear ◽  
Anaerobic Bacteria ◽  
Acute Otitis Media ◽  
Ear Canal ◽  
Aerobic And Anaerobic

Decontamination of the Oral Cavity. Effect of Six Local Anti-Microbial Preparations in Comparison to Water and Parafilm as Controls

1988 ◽  
Vol 16 (2) ◽  
pp. 98-106 ◽  
Author(s):  
C. Matula ◽  
M. Hildebrandt ◽  
G. Nahler
Keyword(s):  
Hydrogen Peroxide ◽  
Oral Cavity ◽  
Anaerobic Bacteria ◽  
Crossover Design ◽  
Double Blind ◽  
Bacterial Counts ◽  
Aerobic And Anaerobic ◽  
Cavity Effect ◽  
Bacterial Effect ◽  
Observation Period

Six preparations (four liquid and two solid) were tested in a double-blind crossover design for their anti-bacterial effect on aerobic and anaerobic bacteria in saliva of 12 volunteers. The four liquid preparations contained either tyrothricin, hexetidine, hydrogen peroxide or ethanol and were tested against a rinse with water as control. The two solid preparations, in the form of lozenges, contained tyrothricin in doses of 4 or 10 mg and were tested against parafilm. A single rinse with either tyrothricin or hexetidine resulted in a significant reduction of aerobic and anaerobic bacteria in saliva which lasted for the whole 1h observation period and was considerably more effective than hydrogen peroxide or ethanol. Similar results were seen with the solid preparations. The lozenge containing 10 mg tyrothricin was the most effective and was the only formulation capable of reducing oral aerobic bacterial counts by a factor of approximately 100.

scholarly journals Beta-Lactamase Activity in Anaerobic Bacteria

1976 ◽  
Vol 10 (1) ◽  
pp. 106-111 ◽  
Author(s):  
A. E. Weinrich ◽  
V. E. D. Bene
Keyword(s):  
Anaerobic Bacteria ◽  
Beta Lactamase

scholarly journals In Vitro and In Vivo Antibacterial Activities of a Novel Glycylcycline, the 9-t-Butylglycylamido Derivative of Minocycline (GAR-936)

1999 ◽  
Vol 43 (4) ◽  
pp. 738-744 ◽  
Author(s):  
P. J. Petersen ◽  
N. V. Jacobus ◽  
W. J. Weiss ◽  
P. E. Sum ◽  
R. T. Testa
Keyword(s):  
Anaerobic Bacteria ◽  
Tetracycline Resistance ◽  
Protective Effects ◽  
Gram Negative ◽  
E Coli ◽  
Resistance Determinants ◽  
Aerobic And Anaerobic ◽  
Ribosomal Protection

ABSTRACT The 9-t-butylglycylamido derivative of minocycline (TBG-MINO) is a recently synthesized member of a novel group of antibiotics, the glycylcyclines. This new derivative, like the first glycylcyclines, theN,N-dimethylglycylamido derivative of minocycline and 6-demethyl-6-deoxytetracycline, possesses activity against bacterial isolates containing the two major determinants responsible for tetracycline resistance: ribosomal protection and active efflux. The in vitro activities of TBG-MINO and the comparative agents were evaluated against strains with characterized tetracycline resistance as well as a spectrum of recent clinical aerobic and anaerobic gram-positive and gram-negative bacteria. TBG-MINO, with an MIC range of 0.25 to 0.5 μg/ml, showed good activity against strains expressing tet(M) (ribosomal protection), tet(A), tet(B),tet(C), tet(D), and tet(K) (efflux resistance determinants). TBG-MINO exhibited similar activity against methicillin-resistant Staphylococcus aureus (MRSA), penicillin-resistant streptococci, and vancomycin-resistant enterococci (MICs at which 90% of strains are inhibited, ≤0.5 μg/ml). TBG-MINO exhibited activity against a wide diversity of gram-negative aerobic and anaerobic bacteria, most of which were less susceptible to tetracycline and minocycline. The in vivo protective effects of TBG-MINO were examined against acute lethal infections in mice caused by Escherichia coli, S. aureus, andStreptococcus pneumoniae isolates. TBG-MINO, administered intravenously, demonstrated efficacy against infections caused byS. aureus including MRSA strains and strains containingtet(K) or tet(M) resistance determinants (median effective doses [ED50s], 0.79 to 2.3 mg/kg of body weight). TBG-MINO demonstrated efficacy against infections caused by tetracycline-sensitive E. coli strains as well asE. coli strains containing either tet(M) or the efflux determinant tet(A), tet(B), ortet(C) (ED50s, 1.5 to 3.5 mg/kg). Overall, TBG-MINO shows antibacterial activity against a wide spectrum of gram-positive and gram-negative aerobic and anaerobic bacteria including strains resistant to other chemotherapeutic agents. The in vivo protective effects, especially against infections caused by resistant bacteria, corresponded with the in vitro activity of TBG-MINO.

scholarly journals Bacteriological Findings within Internal Implant Hole Following Flapless Implant Placement

2018 ◽  
Vol 30 (3) ◽  
pp. 13-16
Author(s):  
Khudair A Abd ◽  
Basima G Ali ◽  
Abbas S AL-Mizraqchi
Keyword(s):  
Health Status ◽  
Anaerobic Bacteria ◽  
Bacterial Count ◽  
Oral Health Status ◽  
Viable Count ◽  
Screw Hole ◽  
Implant Placement ◽  
Significant Difference ◽  
Edentulous Patients ◽  
Aerobic And Anaerobic

Back ground: Microbial penetration inside the implant's internal hole creates a bacterial reservoir that is related with an area of inflamed connective tissue opposite the fixture-abutment junction and this can affect the health of the peri-implant tissue. Aims of the study: Evaluate the types aerobic and anaerobic bacterial count-percentage and difference between Aerobic and Anaerobic microflora in the implant screw hole three months after implant placement. Monitor the periodontal health status of all patients, throughout the study. Material and methods: Study methodology; Eight partially edentulous patients received 20 dental implants and these implants done with flapless surgical procedure. All patients examined clinically to determined their oral health status by examination of their plaque index, Gingival index and Bleeding on probing, each two weeks for 90 days (8visits) throughout the study period. Three months after that, the plaque sample collected from the internal hole of fixture and transfer to bacterial investigation and assessment the amount of anaerobic and aerobic bacteria. Results: Although the anaerobic viable count is higher than that of aerobic, but with statistically not significant difference between those counts (P>0.05). Keywords: screw hole, flapless surgical implant placement, bacterial count, Aerobic and Anaerobic bacteria

scholarly journals Microbiological, histological, technological, and toxicological preliminary study of dog's canned food

2018 ◽  
Vol 49 (2) ◽  
pp. 110
Author(s):  
S. RANTOS (Σ. ΡΑΝΤΟΣ) ◽  
L. PANTOULAS (Λ. ΠΑΝΤΟΥΛΑΣ) ◽  
I. SARAKATSIANOS (Ι. ΣΑΡΑΚΑΤΣΙΑΝΟΣ) ◽  
G. ROZOS (Γ. ΡΟΖΟΣ) ◽  
N. PAPAIOANNOU (Ν. ΠAΠΑΪΩΑNNOΥ)
Keyword(s):  
Connective Tissue ◽  
Water Activity ◽  
Anaerobic Bacteria ◽  
Full Agreement ◽  
Canned Food ◽  
Lead And Cadmium ◽  
Preliminary Study ◽  
Soya Proteins ◽  
Aerobic And Anaerobic

During this study canned food for dogs of six firms were investigated (42 samples totally). Microbiologically the following parameters were checked: the commercial sterility, the water activity (aw), the pH, the presence of aerobic and anaerobic bacteria after incubation of tins in 25 ° C for 28 days, 32 ° C for 21 days and 55 ' C for 8 days. Histologically, the existence of different kinds of tissues was examined. Technologically Weende's analysis and separation of the tin's content were made. Toxicologically, the concentration of lead and cadmium were countered. The results showed that dog's canned food, in Greece, are supersterillised. Muscular, adipose and connective tissue are used for their production as well as byproducts and soya proteins by some firms. The results of Weende's analysis are in full agreement with the amount of nutriments that they were written on tins' labels.

Bacteriology of Adenoids and Tonsils in Children with Recurrent Adenotonsillitis

2001 ◽  
Vol 110 (9) ◽  
pp. 844-848 ◽  
Author(s):  
Itzhak Brook ◽  
Kiran Shah
Keyword(s):  
Candida Albicans ◽  
Haemophilus Influenzae ◽  
Mixed Infection ◽  
Anaerobic Bacteria ◽  
Recurrent Group ◽  
Potential Source ◽  
History Of ◽  
Group A ◽  
Aerobic And Anaerobic

Adenoids and tonsils electively removed from 25 children with a history of recurrent group A β-hemolytic streptococci (GABHS) adenotonsillitis were cultured for aerobic and anaerobic bacteria. Two hundred twenty-four organisms (112 aerobes and facultatives, 110 anaerobes, and 2 Candida albicans) were isolated from the tonsils, and 229 (111 aerobes and facultatives and 118 anaerobes) were isolated from the adenoids. Mixed infection was present in all instances, with an average of 9.1 isolates per specimen. The predominant aerobes were Streptococcus sp, Haemophilus influenzae, and GABHS, and the prevalent anaerobes were Peptostreptococcus, Prevotella, and Fusobacterium spp. β-Lactamase-producing bacteria were detected in 72 isolates recovered from 22 tonsils (88%) and in 74 isolates recovered from 21 adenoids (84%). Discrepancies in the organisms recovered were found between the tonsils and adenoids. Of the aerobic isolates, 20% were isolated only in tonsils and 18% only in adenoids. Of the anaerobes, 20% were found only in tonsils and 26% only in adenoids. This study demonstrates a polymicrobial aerobic-anaerobic flora in both adenoids and tonsils, and discrepancies in recovery of pathogens such as GABHS. The adenoids may serve as a potential source of tonsillitis caused by GABHS.

A Practical Technique for Tympanocentesis for Culturing Aerobic and Anaerobic Bacteria

PEDIATRICS ◽  
1980 ◽  
Vol 65 (3) ◽  
pp. 626-627
Author(s):  
Itzhak Brook
Keyword(s):  
Middle Ear ◽  
Negative Pressure ◽  
Anaerobic Bacteria ◽  
Acute Otitis Media ◽  
Clinical Settings ◽  
Middle Ear Fluid ◽  
Simplified Method ◽  
Medical Indications ◽  
Aerobic And Anaerobic

Tympanocentesis is a method frequently used to aspirate middle ear fluid from infected ears. There are some medical indications to perform tympanocentesis in certain clinical settings.1 This procedure could be beneficial in some patients in which determination of the etiology of the acute otitis media and relieving the pain and acute symptoms is of importance. The instrument frequently used by otolaryngologists is the Senturia ear specimen collector (Storz Instrument Company, St Louis) which operates by controlled suctioning using an external negative pressure. A simplified method for performing myringotomy and tumpanocentesis2 that avoids the use of complicated equipment and prevents contamination of the specimen is presented here.

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