scholarly journals A quick and low-intensity method for oral administration to large numbers of mice: A possible alternative to oral gavage

2021 ◽  
pp. 002367722110352
Author(s):  
Tiago Neto ◽  
Ana I Faustino-Rocha ◽  
Rui M Gil da Costa ◽  
Rui Medeiros ◽  
Paula A Oliveira

Oral administration of medication to experimental animals is a cause of significant stress. When coupled to animals who are already under strenuous circumstances due to the disease being modelled, there is a significant risk for increased morbidity and mortality, thus influencing the results. Faced with these constraints, a low-intensity method for oral administration was developed, based solely on the natural behaviour of the animals and minimal conditioning, in which precise doses of medication were administered in a locally available, standard wheat cookie fragment, providing both a palatable vehicle and an absorbent matrix for the medication. Fast administration to large numbers of animals was thus achieved, safeguarding the animals’ welfare and ensuring ease of handling. This method is a promising alternative to oral gavage in pre-clinical drug studies with laboratory mice.

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Shaimaa H Mahmoud ◽  
Asmaa M Elshaer ◽  
Dalia A El-Waseef ◽  
Dina M Erfan ◽  
Enas S Nabih ◽  
...  

Abstract Background Many theories tried to connect liver and gut as they have close anatomical, metabolic, and immunologic connections so exposure to large numbers of foreign molecules coming from the gastrointestinal tract via the portal vein like the bacterial components and other products may lead to liver damage by disturbing intestinal barrier and permeability which can be induced by indomethacin, one of non-steroidal anti inflammatory drugs(NSAIDs). Aim To investigate whether liver injury is linked to disturbance in intestinal permeability or not. Methodology Indomethacin was used to induce dysbiosis in male Wistar rats which were divided into two groups: Naive and Indomethacin groups. The later was received indomethacin (3mg\kg\day) by oral gavage. Liver function tests, micrbiological and histological studies were performed. Results Indomethacin induced elevation in liver enzymes, bacteremia and histological changes as compared to vehicle group. Conclusions Indomethacin induced dysbiosis with disturbance in the intestinal permeability that yield to hepatic injury.


2017 ◽  
Vol 2 (2) ◽  
Author(s):  
David Mills

<p>Patient values represents one of the three cornerstones of contemporary human EBM definitions, and are considered vital to both protect the patient from the tyranny of unsuitable treatment and to ensure the individual is central in clinical decisions. They comprise “unique preferences, concerns and expectations of each patient” (Sackett et al 1996). By stark contrast, EBVM replaces individual patient values with “circumstances of each patient, and the circumstances and values of the owner/carer” (CEVM 2015). There is a danger that in such a conception, animal welfare may not be prioritised in EBVM practice.</p><p>Animal patients are not philosophically or scientifically likely to possess the complex values of humans, but it is conceivable that they possess ‘values’ in the context of veterinary interventions, such as preferences, wants, needs and desires; indeed these underlie much animal welfare science. They may be basic, such as the avoidance of suffering and a desire to continue life, or more complex, such as a desire to maximise pleasure or natural behaviour. They are likely to be individual and dynamic. They may be defined as the unique dynamic mental preference state of an animal during the period of veterinary medical or surgical intervention, including avoidance of suffering and maximisation of health and pleasure.</p><p>EBVM’s current consideration of patient values only extends to demonstrating effectiveness, or not, of an intervention for an animal. Any intervention involves some physical or mental welfare compromise for animal patients, which may be severe; EBVM aims to ensure this harm 'cost' is consistently outweighed by therapeutic 'benefit'. However, in current EBVM the evidence is often weak, incomplete, contradictory, fuzzy, non-existent or inapplicable, meaning there is a significant risk of causing harm to the patient’s welfare when practising EBVM. This stands in opposition to a veterinary surgeon’s oath and ethical imperative to do no harm, and to do the best for her patients’ welfare (RCVS 2015).</p><p>At what point does the risk become ethically unacceptable? The answer comprises both philosophical considerations, and empirical, critically anthropomorphic attributions of values to animals. Such conceptions and techniques are not currently explicitly expounded in EBVM research or practice. Rather than being marginalised or excluded from definitions of EBVM, this presentation will argue that patient values should be the central, primary and overriding consideration in both its practice and research if EBVM is to be an ethically acceptable pursuit.</p><br /> <img src="https://www.veterinaryevidence.org/rcvskmod/icons/oa-icon.jpg" alt="Open Access" />


Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2631
Author(s):  
Abdullahi Aliyu ◽  
Mohd Rosly Shaari ◽  
Nurul Syahirah Ahmad Sayuti ◽  
Mohd Farhan Hanif Reduan ◽  
Shanmugavelu Sithambaram ◽  
...  

This study investigated the leaves of Clinacanthus nutans for its bioactive compounds and acute and subacute toxicity effects of C. nutans ethanolic leaf extract (CELE) on blood, liver and kidneys of ICR mice. A total of 10 8-week-old female mice were divided into groups A (control) and B (2000 mg/kg) for the acute toxicity study. A single dose of 2000 mg/kg was administered to group B through oral gavage and mice were monitored for 14 days. In the subacute toxicity study, mice were divided into five groups: A (control), B (125 mg/kg), C (250 mg/kg), D (500 mg/kg) and E (1000 mg/kg). The extract was administered daily for 28 days via oral gavage. The mice were sacrificed, and samples were collected for analyses. Myricetin, orientin, isoorientin, vitexin, isovitexin, isookanin, apigenin and ferulic acid were identified in the extract. Twenty-eight days of continuous oral administration revealed significant increases (p < 0.05) in creatinine, ALT and moderate hepatic and renal necrosis in groups D and E. The study concluded that the lethal dose (LD50) of CELE in mice is greater than 2000 mg/kg and that repeated oral administrations of CELE for 28 days induced hepatic and renal toxicities at 1000 mg/kg in female ICR mice.


2003 ◽  
Vol 38 (2) ◽  
pp. 140-143 ◽  
Author(s):  
Christine D. Sybert

This article describes the process and results of employing an Investigational Drug Service Pharmacist (IDSP) to coordinate pharmacy support for clinical drug studies. In 1998, a Clinical Research Center (CRC) was established to coordinate all research at a 407-bed Baltimore teaching hospital, resulting in an increase in studies requiring pharmacy support. By the end of 1998, there was a backlog of nine studies awaiting review for feasibility and impact on pharmacy resources. A lack of communication between departments further impeded the process. In March 1999, a part-time pharmacist was hired to coordinate all drug studies; the pharmacist's salary was paid by the CRC. Primary responsibilities of the position were protocol review, staff education, and drug accountability. Dispensing was still handled by staff pharmacists. After three years, results were as follows: 72 studies were reviewed, 41 were opened for enrollment, 8 were in preparation, 16 were completed. Two studies alone have saved the pharmacy department over $80,000 in drug acquisition costs. The efforts of the IDSP have helped the CRC to more than double its growth, with gross income from studies of $533,712 in 2001 compared with only $237,663 in 1998. The IDSP has improved communication between the CRC and pharmacy and expedited study initiation. It has also made the CRC more attractive to prospective sponsors, since one pharmacist works directly with study coordinators. With more physicians interested in carrying out research and hospital budgets becoming tighter, the part-time IDSP position represents an important means of supporting these endeavors without increasing fiscal burdens.


2015 ◽  
Vol 41 (03) ◽  
pp. 213-218
Author(s):  
Chia-Yih Wang ◽  
Shi-Yuan Sheu ◽  
Yi-Chih Lei ◽  
Jiann-Hsiung Wang ◽  
Ming-Huang Chang ◽  
...  

Amoxicillin is an antibiotic which belongs to the group of penicillins. It is approved in Taiwan for treating bacterial infections caused by Streptococcus sp. and Photobacterium sp. in anguilliformes, perciformes and salmoniformes. The pharmacokinetics of amoxicillin were determined in pompano following oral administration of a single dose of 40 mg/kg. Residue studies were performed to determine residues in liver and kidney tissues of healthy fish after oral gavage of amoxicillin at a daily dose of 40 mg/kg for five consecutive days. Amoxicillin residues were analyzed by HPLC using Hypersil-100 C18 column (150 mm × 4.6 mm i.d.) and mobile phase consists of 10 mM K2HPO4(pH 8.5) with acetonitrile (80:20, v/v), at a flow rate of 1 mL/min. The effluent was monitored using a fluorescence detector set as 358 and 440 nm as excitation and emission wavelengths. Following a single oral dose, amoxicillin residues in 0.5 h post-dosing pompano were at a maximum of 6.17 μg/g in liver and 4.27 μg/g in kidney; the concentration of amoxicillin in liver and kidney declined with half-lives of 18.3 and 12.0 h. Amoxicillin residues in pompano liver and kidney tissues were proved to be under the MRL, 0.5 ug/g (liver and kidney) after a withdrawal period of five days.


Gerontology ◽  
2017 ◽  
Vol 64 (2) ◽  
pp. 172-187 ◽  
Author(s):  
Michaela Weber ◽  
Nacera Belala ◽  
Lindy Clemson ◽  
Elisabeth Boulton ◽  
Helen Hawley-Hague ◽  
...  

Background: Traditionally, exercise programmes for improving functional performance and reducing falls are organised as structured sessions. An alternative approach of integrating functional exercises into everyday tasks has emerged in recent years. Objectives: Summarising the current evidence for the feasibility and effectiveness of interventions integrating functional exercise into daily life. Methods: A systematic literature search was conducted including articles based on the following criteria: (1) individuals ≥60 years; (2) intervention studies of randomised controlled trials (RCTs) and non-randomised studies (NRS); (3) using a lifestyle-integrated approach; (4) using functional exercises to improve strength, balance, or physical functioning; and (5) reporting outcomes on feasibility and/or effectiveness. Methodological quality of RCTs was evaluated using the PEDro scale. Results: Of 4,415 articles identified from 6 databases, 14 (6 RCTs) met the inclusion criteria. RCT quality was moderate to good. Intervention concepts included (1) the Lifestyle-integrated Functional Exercise (LiFE) programme integrating exercises into everyday activities and (2) combined programmes using integrated and structured training. Three RCTs evaluated LiFE in community dwellers and reported significantly improved balance, strength, and functional performance compared with controls receiving either no intervention, or low-intensity exercise, or structured exercise. Two of these RCTs reported a significant reduction in fall rate compared with controls receiving either no intervention or low-intensity exercise. Three RCTs compared combined programmes with usual care in institutionalised settings and reported improvements for some (balance, functional performance), but not all (strength, falls) outcomes. NRS showed behavioural change related to LiFE and feasibility in more impaired populations. One NRS comparing a combined home-based programme to a gym-based programme reported greater sustainability of effects in the combined programme. Conclusions: This review provides evidence for the effectiveness of integrated training for improving motor performances in older adults. Single studies suggest advantages of integrated compared with structured training. Combined programmes are positively evaluated in institutionalised settings, while little evidence exists in other populations. In summary, the approach of integrating functional exercise into daily life represents a promising alternative or complement to structured exercise programmes. However, more RCTs are needed to evaluate this concept in different target populations and the potential for inducing behavioural change.


2007 ◽  
Vol 2007 ◽  
pp. 34-34
Author(s):  
JJ Cooper ◽  
J. Campbell ◽  
P. Harris

Activity patterns of domesticated animals have largely focussed on hours of daylight and relatively few studies include detailed observations of night time activity. This has the potential to overlook behaviours of significance to the assessment of welfare. For example, stereotypic activities in laboratory mice are largely confined to dark periods, and consequently are not commonly reported by daytime laboratory workers. Use of low light video cameras coupled with infra-red or low intensity lighting now makes observation over entire light-dark cycle practical, whilst minimising disturbance to the sampled population. This paper describes the activity patterns of stabled horses over 24 hour periods. These observations can then be used as baseline for investigating the effects of changes to the stable environment on horses’ behaviour and welfare.


PEDIATRICS ◽  
1975 ◽  
Vol 56 (5s) ◽  
pp. 935-936
Author(s):  
Joseph Oren

Exercise-induced bronchspasm (EIB), as opposed to asthma precipitated by exercise, is a valuable laboratory tool in the study of the newer pharmacologic agents being developed for asthma therapy. EIB is uniquely suited for the screening of agents with cromolyn-sodium-like activity prior to extended clinical drug studies in large patient populations. To be used meaningfully, the challenge technique must be subjected to rigorous controls, standardization, and evaluation in preselected patient populations. Because of the inherent variability of the method, careful study design and the use of double-blind statistical controls are necessary to minimize misleading placebo effects.


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