scholarly journals Roles of macrophage migration inhibitory factor in polymyositis: Inflammation and regeneration

2017 ◽  
Vol 46 (2) ◽  
pp. 732-738
Author(s):  
Yu-Qiong Zou ◽  
Wei-Dong Jin ◽  
Ya-Song Li
Keyword(s):  
Creatine Kinase ◽  
Serum Concentration ◽  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Early Stage ◽  
Inhibitory Factor ◽  
Enzyme Linked Immunosorbent Assay ◽  
Macrophage Migration ◽  
Healthy Controls ◽  
Serum Samples

Objective To elucidate the clinical significance of macrophage migration inhibitory factor (MIF) serum concentration in patients with polymyositis. Methods Thirty-six patients with polymyositis were enrolled. Serum samples were obtained and stored to detect MIF and interleukin (IL)-6 using commercially available enzyme-linked immunosorbent assay kits. The relationships between these cytokines and clinical data were analyzed. Results The serum MIF concentration was significantly lower in patients in remission (34.74 ± 17.75) and in healthy controls (38.87 ± 9.30 ng/ml) than that in patients with active polymyositis (50.04 ± 23.84 ng/ml). There were no significant differences between healthy controls and patients in remission. The serum IL-6 concentration in patients with active polymyositis (19.67 ± 7.16 pg/ml) was significantly higher than that in patients in remission (15.81 ± 4.00 pg/ml) and controls (8.14 ± 3.71 pg/ml). The serum IL-6 concentration was negatively correlated with the serum MIF concentration (r = −0.283). No relationship was found between the serum MIF concentration and glucocorticoid dose. The MIF concentration peaked twice during treatment when the creatine kinase concentration was decreasing. Conclusion MIF and IL-6 play important roles in the inflammation associated with polymyositis. MIF might also be involved in the early stage of regeneration in polymyositis. MIF may thus serve as a biomarker of disease activity and outcome.

scholarly journals Macrophage Migration Inhibitory Factor as a Novel Biomarker of Portopulmonary Hypertension

2016 ◽  
Vol 6 (4) ◽  
pp. 498-507 ◽  
Author(s):  
Hilary M. DuBrock ◽  
Josanna M. Rodriguez-Lopez ◽  
Barbara L. LeVarge ◽  
Michael P. Curry ◽  
Paul A. VanderLaan ◽  
...  
Keyword(s):  
Liver Disease ◽  
Migration Inhibitory Factor ◽  
Predictive Value ◽  
Pulmonary Circulation ◽  
Macrophage Migration Inhibitory Factor ◽  
Inhibitory Factor ◽  
Enzyme Linked Immunosorbent Assay ◽  
Heart Catheterization ◽  
Macrophage Migration ◽  
Portopulmonary Hypertension

Portopulmonary hypertension (POPH) is a poorly understood complication of liver disease associated with significant morbidity and mortality. We sought to identify novel biomarkers of POPH disease presence and severity. We performed a prospective, multicenter, case-control study involving patients with liver disease undergoing right heart catheterization. POPH cases were defined as a mean pulmonary arterial pressure (mPAP) ≥25 mmHg and pulmonary vascular resistance (PVR) >240 dynes·s·cm−5. Plasma samples were collected from the systemic and pulmonary circulation, and antibody microarray was used to identify biomarkers. Characterization and validation of a candidate cytokine, macrophage migration inhibitory factor (MIF), was performed using enzyme-linked immunosorbent assay. Continuous variables were compared using a Mann-Whitney U test and correlated with disease severity using Spearman correlation. MIF levels were elevated in both the systemic and pulmonary circulation in patients with POPH compared with controls (median MIF level [interquartile range] in systemic circulation: 46.68 ng/mL [32.31–76.04] vs. 31.19 ng/mL [26.92–42.17], P = 0.009; in pulmonary circulation: 49.59 ng/mL [35.90–108.80] vs. 37.78 [21.78–45.53], P = 0.002). In patients with POPH, MIF levels were positively correlated with PVR ( r = 0.58, P = 0.006) and inversely correlated with cardiac output ( r = −0.57, P = 0.007). MIF >60 ng/mL or tricuspid regurgitation gradient >50 mmHg had a 92% sensitivity and specificity for the diagnosis of POPH, with a positive predictive value of 86% and a negative predictive value of 96%. MIF is a promising novel biomarker of POPH disease presence and severity in patients with liver disease and portal hypertension.

scholarly journals Role of Macrophage Migration Inhibitory Factor in Otitis Media with Effusion in Adults

2003 ◽  
Vol 10 (3) ◽  
pp. 417-422 ◽  
Author(s):  
Shin Kariya ◽  
Mitsuhiro Okano ◽  
Katsuya Aoji ◽  
Michiya Kosaka ◽  
Emiko Chikumoto ◽  
...  
Keyword(s):  
Otitis Media ◽  
Middle Ear ◽  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Otitis Media With Effusion ◽  
Fluid Collection ◽  
Inhibitory Factor ◽  
Enzyme Linked Immunosorbent Assay ◽  
Macrophage Migration ◽  
Tnf Α

ABSTRACT Otitis media with effusion (OME) is one of the most common ear diseases. Bacterial endotoxins and several inflammatory cytokines appear to be involved in the pathogenesis of OME in children; however, little is known of the immunological aspects of the onset of OME in adults. We sought to determine the presence of macrophage migration inhibitory factor (MIF) as well as interleukin 1β (IL-1β), tumor necrosis factor alpha (TNF-α), RANTES (regulated upon activation, normal T-cell expressed and presumably secreted), and endotoxin in middle ear effusions (MEEs) from adult patients with OME. In addition, the levels of MIF in MEEs from adults and children were compared. MEE was obtained from 95 adults and 11 children. The levels of MIF, IL-1β, TNF-α, and RANTES were determined by enzyme-linked immunosorbent assay, and the concentrations of endotoxin and total protein were determined by the Endospec assay and bicinchoninic acid assay, respectively. MIF was detected in 97.9% of the MEEs from adults, while endotoxin, IL-1β, TNF-α, and RANTES were detected in 96.8, 12.6, 5.3, and 43.9%, respectively. In addition, the level of MIF was significantly higher than those of endotoxin, IL-1β, and TNF-α. A positive correlation between the levels of MIF and endotoxin was observed. MIF and endotoxin were detected in 81.8 and 72.7%, respectively, of the MEEs from the children. The level of MIF was significantly higher in the children, and conversely that of endotoxin was significantly higher in the adults. These results suggest that the interaction between MIF and endotoxin may promote fluid collection in the middle ear, particularly in adults.

scholarly journals Efficacy of Sertraline Plus Placebo or Add-On Celecoxib in Major Depressive Disorder: Macrophage Migration Inhibitory Factor as a Promising Biomarker for Remission After Sertraline—Results From a Randomized Controlled Clinical Trial

2021 ◽  
Vol 12 ◽  
Author(s):  
Maria S. Simon ◽  
Bianka Burger ◽  
Elif Weidinger ◽  
Gara Arteaga-Henríquez ◽  
Peter Zill ◽  
...  
Keyword(s):  
Major Depressive Disorder ◽  
Depressive Disorder ◽  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Inhibitory Factor ◽  
Enzyme Linked Immunosorbent Assay ◽  
Depression Symptoms ◽  
Macrophage Migration ◽  
Major Depressive ◽  
Baseline Levels

Introduction: Previous research delivers strong indications that inflammatory activation leads to treatment resistance in a subgroup of patients with Major Depressive Disorder (MDD). Thus, tailored interventions are needed. The present study aimed to find potential biomarkers that may enable patients to be stratified according to immune activation.Methods: A phase IIa randomized placebo-controlled trial was performed to assess levels of inflammatory compounds in responders/remitters and non-responders/non-remitters to sertraline plus celecoxib (n = 20) and sertraline plus placebo (n = 23). Levels of macrophage migration inhibitory factor, neopterin, and tumor necrosis factor alpha were determined by enzyme-linked immunosorbent assay; response and remission were measured by reduction of the Montgomery Åsberg Depression Rating Scale score.Results: Both treatment groups showed a significant decline in depression symptoms, but no difference was found between groups. A clear pattern emerged only for macrophage migration inhibitory factor: placebo remitters showed significantly lower baseline levels than non-remitters (a similar trend was seen in responders and non-responders) while celecoxib responders showed a trend for higher baseline levels than non-responders.Conclusion: Small subsample sizes are a notable limitation, wherefore results are preliminary. However, the present study provides novel insights by suggesting macrophage migration inhibitory factor as a promising biomarker for treatment choice.The trial was registered in EU Clinical Trials Register (EU-CTR): https://www.clinicaltrialsregister.eu/ctr-search/trial/2009-011990-34/DE, EudraCT-No.: 2009-011990-34.

scholarly journals Macrophage Migration Inhibitory Factor Levels in Gingival Crevicular Fluid, Saliva, and Serum of Chronic Periodontitis Patients

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Yveth Marlene Ortiz-García ◽  
Trinidad García-Iglesias ◽  
Gabriela Morales-Velazquez ◽  
Blanca Patricia Lazalde-Ramos ◽  
Guillermo Moisés Zúñiga-González ◽  
...  
Keyword(s):  
Migration Inhibitory Factor ◽  
Chronic Periodontitis ◽  
Macrophage Migration Inhibitory Factor ◽  
Gingival Crevicular Fluid ◽  
Clinical Signs ◽  
Inhibitory Factor ◽  
Enzyme Linked Immunosorbent Assay ◽  
Macrophage Migration ◽  
Cross Sectional ◽  
Crevicular Fluid

Chronic periodontitis (CP) is an infection that affects the teeth supporting structure. Macrophage migration inhibitory factor (MIF) is an important effector cytokine of the innate immune system. Due to its functional characteristics, MIF may be involved in the immunopathology of CP. The aim of the present study was to evaluate MIF levels in gingival crevicular fluid (GCF), saliva, and serum of CP patients. A cross-sectional study was conducted on 60 subjects divided into two groups: subjects with CP (n= 30) and periodontally healthy subjects without CP (n=30). MIF was quantified in GCF, saliva, and serum of all participants by enzyme-linked immunosorbent assay. MIF concentrations were higher in GCF, saliva, and serum in the group with CP compared with the group without CP and a higher MIF concentration was observed in GCF (p=0.001) and saliva (p=0.009) in the group with CP. MIF intragroup comparisons between fluids demonstrated significant high levels of MIF in saliva compared with GCF and serum in both study groups (p<0.05). A positive correlation was found between clinical signs and MIF concentration in GCF (p<0.05). There is an association between the MIF and the clinical signs of the disease. Therefore, MIF could have an important role in the pathology and progression of CP.

scholarly journals Macrophage migration inhibitory factor (MIF): Genetic evidence for participation in early onset and early stage rheumatoid arthritis

Cytokine ◽  
2013 ◽  
Vol 61 (3) ◽  
pp. 759-765 ◽  
Author(s):  
M.A. Llamas-Covarrubias ◽  
Y. Valle ◽  
R. Bucala ◽  
R.E. Navarro-Hernández ◽  
C.A. Palafox-Sánchez ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Migration Inhibitory Factor ◽  
Early Onset ◽  
Macrophage Migration Inhibitory Factor ◽  
Early Stage ◽  
Genetic Evidence ◽  
Inhibitory Factor ◽  
Macrophage Migration

scholarly journals Factors Associated With Macrophage Migration Inhibitory Factor in Neonates: a Pilot Study

2021 ◽  
Author(s):  
Ji Sook Park ◽  
Jin Su Jun ◽  
Ji-Hyun Seo ◽  
Jae Young Lim ◽  
Chan-Hoo Park ◽  
...  
Keyword(s):  
Migration Inhibitory Factor ◽  
Associated Factors ◽  
Macrophage Migration Inhibitory Factor ◽  
Inhibitory Factor ◽  
Preterm Neonates ◽  
Enzyme Linked Immunosorbent Assay ◽  
Macrophage Migration ◽  
Clinical Factors ◽  
Term Neonates ◽  
Factors Associated

Abstract Objective: The study was to conduct an investigation of associated factor with macrophage migration inhibitory factor (MIF) in neonates.Results: MIF was measured by Enzyme-linked Immunosorbent Assay (ELISA) using sera obtained from sick neonates on the median postnatal 2.0nd day. Clinical details were reviewed from medical record and grouped into preterm and near or full-term neonates based on 34 weeks of gestation at birth. Statistical analyses were performed between MIF concentration and clinical factors. In total, 77 neonates consisted of preterm (n = 42) and term neonates (n = 35) were included. The median value of plasma MIF was higher in preterm neonates (7,037.6 pg/mL, IQR: 3,285.2-12,267.4) than in the others (3,968.8 pg/mL, IQR: 2,566.4-6,995.2, P = 0.013). Among 42 preterm neonates, those with necrotizing enterocolitis (NEC) in later had higher MIF concentration (12,170.9 pg/mL, IQR: 8,353.3-23,537.7, n = 9) compared with the others without NEC (5,189.6 pg/mL, IQR: 3,220.2-9,097.9, n = 33, P = 0.016). Associated factors of higher MIF in neonates were preterm neonates and NEC.

Role of Macrophage Migration Inhibitory Factor in Paranasal Sinus Mucocele

2005 ◽  
Vol 19 (6) ◽  
pp. 554-559 ◽  
Author(s):  
Shin Kariya ◽  
Mitsuhiro Okano ◽  
Katsuya Aoji ◽  
Tomoko Nakashima ◽  
Norio Kasai ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Paranasal Sinus ◽  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Tumor Necrosis ◽  
Inhibitory Factor ◽  
Interleukin 1Β ◽  
Enzyme Linked Immunosorbent Assay ◽  
Macrophage Migration ◽  
Necrosis Factor

Background Little is known about the immunologic aspects and the pathogenesis of the paranasal sinus mucocele. Methods The fluids of paranasal sinus mucoceles were obtained from 12 subjects. The concentration of macrophage migration inhibitory factor (MIF), interleukin 1β, tumor necrosis factor a, and regulated on activation normal T cell expressed and secreted (RANTES) were determined by enzyme-linked immunosorbent assay, and the levels of endotoxin were detected with kinetic Turbidimetric Assay. Results MIF and endotoxin were detected in the fluid of all samples, whereas interleukin-1β and RANTES were detected in 1 and 3 subjects out of 12 samples. Tumor necrosis factor a was not detected in any of the samples. A significant positive correlation between the levels of MIF and the period with symptoms such as pain, swelling of face, and visual disturbance was observed. Conclusion These findings suggest that MIF and endotoxin may play an important role in the pathogenesis of paranasal sinus mucocele. MIF may be an important factor causing the development and exacerbation of the disease.

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