Biocompatibility and biodegradation properties of polycaprolactone/polydioxanone composite scaffolds prepared by blend or co-electrospinning

2019 ◽  
Vol 34 (2) ◽  
pp. 115-130 ◽  
Author(s):  
Xin Zhou ◽  
Yiwa Pan ◽  
Ruihua Liu ◽  
Xin Luo ◽  
Xianyan Zeng ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Mechanical Strength ◽  
Structural Characteristics ◽  
Tissue Engineering Scaffold ◽  
Morphological Properties ◽  
Composite Scaffolds ◽  
Electrospun Scaffolds ◽  
Hybrid Scaffolds

Electrospun polymer scaffolds are regarded as an ideal tissue engineering scaffold due to similar morphological properties with the native extracellular matrix. Among these, polycaprolactone is widely used to fabricate electrospun fibrous scaffolds due to its excellent biocompatibility, good mechanical properties, and ease of manufacture. However, its low biodegradation rate has a negative influence on its application in tissue engineering scaffold. To address this issue, this study prepared hybrid scaffolds composed of polycaprolactone and polydioxanone (a fast-degrading polyether-ester) via either the blend or co-electrospinning. Subsequently, the structural characteristics, mechanical strength, in vitro/vivo degradation, cellularization, and vascularization of two kinds of hybrid scaffolds were evaluated to decide which method is more suitable for producing tissue engineering scaffolds. The incorporation of polydioxanone increased the mechanical strength of both composite scaffolds. Moreover, co-electrospun scaffolds exhibited improved hydrophilicity compared to blend scaffolds. The results of in vitro and in vivo degradation studies showed that the degradation rate of both composite scaffolds was faster than that of neat polycaprolactone scaffolds due to the incorporated polydioxanone component. Especially in co-electrospun scaffolds, the fast degradation of polydioxanone fiber gave rise to larger pore size, thus leading to faster cellularization and better vascularization compared to blend scaffolds. Therefore, co-electrospinning was demonstrated to be superior to blend electrospinning for the preparation of composite scaffolds. Co-electrospun polycaprolactone–polydioxanone scaffolds may be promising candidates for tissue engineering.

Superparamagnetic Core–Shell Electrospun Scaffolds with Sustained Release of IONPs Facilitating in vitro and in vivo Bone Regeneration

2021 ◽  
Author(s):  
Shuying Hu ◽  
Hanbang Chen ◽  
Fang Zhou ◽  
Jun Liu ◽  
Yun Zhu Qian ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Sustained Release ◽  
Mechanical Strength ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Core Shell ◽  
Electrospun Scaffolds ◽  
Dental Implantation

Bone tissue engineering (BTE) is a promising approach to recover insufficient bone in dental implantation. However, the clinical application of BTE scaffolds is limited by their low mechanical strength and...

Biocompatabiltiy of Chitosan/Poly(L-Lactic Acid) Composite Scaffolds with Three-Dimensional Honeycomb Patterned Structure

2011 ◽  
Vol 140 ◽  
pp. 29-33
Author(s):  
Ming Yan Zhao ◽  
Li Hua Li ◽  
Guo Dong Sun ◽  
Chang Ren Zhou
Keyword(s):  
Mechanical Property ◽  
Tissue Engineering ◽  
Lactic Acid ◽  
Three Dimensional ◽  
Tissue Engineering Scaffold ◽  
Good Mechanical Property ◽  
Composite Scaffolds ◽  
Ideal Candidate

Three dimensional (3D) scaffolds provide the necessary support for cells to attach, proliferate and differentiate, and define the overall shape of the tissue engineered transplant. In this study, 3D honeycomb patterned chitosan/poly (L-lactic acid) composite scaffolds fabricated by an easy manipulated technique with good mechanical property and cytocompatability, as demonstrated by a previous study. Here we investigated further the in vitro cytocompatibility and spine regeneration in vivo by implanting the construct into male white rabbits for 4 and 8weeks. Results showed that such a honeycomb patterned scaffolds have a good cytocompatibilty. Also, the rabbit spinal defect was perfectly restored. These findings supported that such a 3D honeycomb patterned scaffold is an ideal candidate for the tissue engineering scaffold.

scholarly journals Effective decellularisation of human saphenous veins for biocompatible arterial tissue engineering applications: Bench optimisation and feasibility in vivo testing

2021 ◽  
Vol 12 ◽  
pp. 204173142098752
Author(s):  
Nadiah S Sulaiman ◽  
Andrew R Bond ◽  
Vito D Bruno ◽  
John Joseph ◽  
Jason L Johnson ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Mechanical Strength ◽  
Vascular Tissue ◽  
Sodium Dodecyl ◽  
Host Cells ◽  
Replacement Model ◽  
In Vivo Testing ◽  
Vascular Scaffolds

Human saphenous vein (hSV) and synthetic grafts are commonly used conduits in vascular grafting, despite high failure rates. Decellularising hSVs (D-hSVs) to produce vascular scaffolds might be an effective alternative. We assessed the effectiveness of a detergent-based method using 0% to 1% sodium dodecyl sulphate (SDS) to decellularise hSV. Decellularisation effectiveness was measured in vitro by nuclear counting, DNA content, residual cell viability, extracellular matrix integrity and mechanical strength. Cytotoxicity was assessed on human and porcine cells. The most effective SDS concentration was used to prepare D-hSV grafts that underwent preliminary in vivo testing using a porcine carotid artery replacement model. Effective decellularisation was achieved with 0.01% SDS, and D-hSVs were biocompatible after seeding. In vivo xeno-transplantation confirmed excellent mechanical strength and biocompatibility with recruitment of host cells without mechanical failure, and a 50% patency rate at 4-weeks. We have developed a simple biocompatible methodology to effectively decellularise hSVs. This could enhance vascular tissue engineering toward future clinical applications.

scholarly journals Full cell infiltration and thick tissue formation in vivo in tailored electrospun scaffolds

2020 ◽  
Author(s):  
Jip Zonderland ◽  
Silvia Rezzola ◽  
David Gomes ◽  
Sandra Camarero Espinosa ◽  
Ana Henriques Ferreira Lourenço ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Fiber Diameter ◽  
Cell Infiltration ◽  
Tissue Formation ◽  
Cell Seeding ◽  
Subcutaneous Implantation ◽  
Electrospun Scaffolds ◽  
Limited Depth

AbstractElectrospun (ESP) scaffolds are a promising type of tissue engineering constructs for large defects with limited depth. To form new functional tissue, the scaffolds need to be infiltrated with cells, which will deposit extracellular matrix. However, due to dense fiber packing and small pores, cell and tissue infiltration of ESP scaffolds is limited. Here, we combine two established methods, increasing fiber diameter and co-spinning sacrificial fibers, to create a porous ESP scaffold that allows robust tissue infiltration. Full cell infiltration across 2 mm thick scaffolds is seen 3 weeks after subcutaneous implantation in rats. After 6 weeks, the ESP scaffolds are almost fully filled with de novo tissue. Cell infiltration and tissue formation in vivo in this thickness has not been previously achieved. In addition, we propose a novel method for in vitro cell seeding to improve cell infiltration and a model to study 3D migration through a fibrous mesh. This easy approach to facilitate cell infiltration further improves previous efforts and could greatly aid tissue engineering approaches utilizing ESP scaffolds.Statement of significanceElectrospinning creates highly porous scaffolds with nano- to micrometer sized fibers and are a promising candidate for a variety of tissue engineering applications. However, smaller fibers also create small pores which are difficult for cells to penetrate, restricting cells to the top layers of the scaffolds. Here, we have improved the cell infiltration by optimizing fiber diameter and by co-spinning a sacrificial polymer. We developed novel culture technique that can be used to improve cell seeding and to study cytokine driven 3D migration through fibrous meshes. After subcutaneous implantation, infiltration of tissue and cells was observed up to throughout up to 2 mm thick scaffolds. This depth of infiltration in vivo had not yet been reported for electrospun scaffolds. The scaffolds we present here can be used for in vitro studies of migration, and for tissue engineering in defects with a large surface area and limited depth.

scholarly journals Ether-Oxygen Containing Electrospun Microfibrous and Sub-Microfibrous Scaffolds Based on Poly(butylene 1,4-cyclohexanedicarboxylate) for Skeletal Muscle Tissue Engineering

2018 ◽  
Vol 19 (10) ◽  
pp. 3212 ◽  
Author(s):  
Nora Bloise ◽  
Emanuele Berardi ◽  
Chiara Gualandi ◽  
Elisa Zaghi ◽  
Matteo Gigli ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Muscle Tissue ◽  
Skeletal Muscle Tissue ◽  
Cell Alignment ◽  
Fibre Direction ◽  
Electrospun Scaffolds ◽  
Fibre Morphology

We report the study of novel biodegradable electrospun scaffolds from poly(butylene 1,4-cyclohexandicarboxylate-co-triethylene cyclohexanedicarboxylate) (P(BCE-co-TECE)) as support for in vitro and in vivo muscle tissue regeneration. We demonstrate that chemical composition, i.e., the amount of TECE co-units (constituted of polyethylene glycol-like moieties), and fibre morphology, i.e., aligned microfibrous or sub-microfibrous scaffolds, are crucial in determining the material biocompatibility. Indeed, the presence of ether linkages influences surface wettability, mechanical properties, hydrolytic degradation rate, and density of cell anchoring points of the studied materials. On the other hand, electrospun scaffolds improve cell adhesion, proliferation, and differentiation by favouring cell alignment along fibre direction (fibre morphology), also allowing for better cell infiltration and oxygen and nutrient diffusion (fibre size). Overall, C2C12 myogenic cells highly differentiated into mature myotubes when cultured on microfibres realised with the copolymer richest in TECE co-units (micro-P73 mat). Lastly, when transplanted in the tibialis anterior muscles of healthy, injured, or dystrophic mice, micro-P73 mat appeared highly vascularised, colonised by murine cells and perfectly integrated with host muscles, thus confirming the suitability of P(BCE-co-TECE) scaffolds as substrates for skeletal muscle tissue engineering.

scholarly journals Poly(3-Hydroxybutyrate)-Multiwalled Carbon Nanotubes Electrospun Scaffolds Modified with Curcumin

Polymers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 2588
Author(s):  
Nader Tanideh ◽  
Negar Azarpira ◽  
Najmeh Sarafraz ◽  
Shahrokh Zare ◽  
Aida Rowshanghiyas ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Tissue Engineering ◽  
Multiwalled Carbon Nanotubes ◽  
Inflammatory Reaction ◽  
3D Structure ◽  
Hydrolytic Degradation ◽  
Multiwalled Carbon ◽  
Electrospun Scaffolds

Appropriate selection of suitable materials and methods is essential for scaffolds fabrication in tissue engineering. The major challenge is to mimic the structure and functions of the extracellular matrix (ECM) of the native tissues. In this study, an optimized 3D structure containing poly(3-hydroxybutyrate) (P3HB), multiwalled carbon nanotubes (MCNTs) and curcumin (CUR) was created by electrospinning a novel biomimetic scaffold. CUR, a natural anti-inflammatory compound, has been selected as a bioactive component to increase the biocompatibility and reduce the potential inflammatory reaction of electrospun scaffolds. The presence of CUR in electrospun scaffolds was confirmed by 1H NMR and Fourier-transform infrared spectroscopy (FTIR). Scanning electron microscopy (SEM) revealed highly interconnected porosity of the obtained 3D structures. Addition of up to 20 wt% CUR has enhanced mechanical properties of the scaffolds. CUR has also promoted in vitro bioactivity and hydrolytic degradation of the electrospun nanofibers. The developed P3HB-MCNT composite scaffolds containing 20 wt% of CUR revealed excellent in vitro cytocompatibility using mesenchymal stem cells and in vivo biocompatibility in rat animal model study. Importantly, the reduced inflammatory reaction in the rat model after 8 weeks of implantation has also been observed for scaffolds modified with CUR. Overall, newly developed P3HB-MCNTs-CUR electrospun scaffolds have demonstrated their high potential for tissue engineering applications.

scholarly journals Excellency of Hydroxyapatite Composite Scaffolds for Bone Tissue Engineering

Biomaterials ◽  
2020 ◽  
Author(s):  
Mohammad Shariful Islam ◽  
Mohammad Abdulla-Al-Mamun ◽  
Alam Khan ◽  
Mitsugu Todo
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Surface Reactivity ◽  
Growth Factor Delivery ◽  
Composite Scaffolds ◽  
In Vivo Experiments ◽  
Hydroxyapatite Composite

The hydroxyapatite [HAp, Ca10(PO4)6(OH)2] has a variety of applications in bone fillers and replacements due to its excellent bioactivity and osteoconductivity. It comprises the main inorganic component of hard tissues. Among the various approaches, a composite approach using several components like biopolymer, gelatin, collagen, and chitosan in the functionalization of scaffolds with HAp has the prospective to be an engineered biomaterial for bone tissue engineering. HAp composite scaffolds have been developed to obtain a material with different functionalities such as surface reactivity, bioactivity, mechanical strength, and capability of drug or growth factor delivery. Several techniques and processes for the synthesis and fabrication of biocompatible HAp composite scaffolds suitable for bone regeneration are addressed here. Further, this chapter described the excellences of various HAp composite scaffolds used in in vitro and in vivo experiments in bone tissue engineering.

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