Genotoxicity in Brazilian coal miners and its associated factors

2017 ◽  
Vol 37 (9) ◽  
pp. 891-900 ◽  
Author(s):  
FMR da Silva Júnior ◽  
RA Tavella ◽  
CLF Fernandes ◽  
MCF Soares ◽  
KA de Almeida ◽  
...  
Keyword(s):  
Coal Mining ◽  
Oral Mucosa ◽  
Associated Factors ◽  
Micronucleus Test ◽  
Coal Extraction ◽  
Control Group ◽  
Coal Miners ◽  
Significant Difference ◽  
Group 2 ◽  
And Control

The present study aims to evaluate the potential genotoxic and associated factors among coal miners, divided by degree of exposure. Blood and buccal smears were collected from 158 workers, who actively participate in different activities in coal mining, and 48 individuals living in the same city but do not have participation in coal mining activities (control group). The workers were divided into three different groups, according to the level of contact with coal extraction. A questionnaire intended to identify factors associated with DNA damage was performed in participants. The results regarding oral mucosa micronucleus test showed a significant difference ( p < 0.001) of the worker groups 1 and 2 in relation to the control group, where the group 1 has a higher degree of exposure to coal than group 2. For the lymphocyte micronucleus test and comet assay, there was no significant difference between the exposed groups and control group. There is an association between the outcome and the fact of living in the municipality of the mining company and the exposure to radiation in the last 12 months. Besides, the multivariate analysis showed an association of the tail moment with radiation exposure in the last 12 months. Thus, the findings of this study reveal genotoxicity in oral mucosa cells of workers exposed to coal and that workers with higher degree of contact with coal have a more pronounced response.

scholarly journals Effect of Oral Losartan on Orthobiologics: Implications for Platelet-Rich Plasma and Bone Marrow Concentrate—A Rabbit Study

2020 ◽  
Vol 21 (19) ◽  
pp. 7374
Author(s):  
Gilberto Y. Nakama ◽  
Sabrina Gonzalez ◽  
Polina Matre ◽  
Xiaodong Mu ◽  
Kaitlyn E. Whitney ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Osteochondral Defect ◽  
Platelet Rich Plasma ◽  
Control Group ◽  
Bone Marrow Concentrate ◽  
Significant Difference ◽  
Losartan Group ◽  
Group 2 ◽  
And Control ◽  
Tgf Β1

Recent efforts have focused on customizing orthobiologics, such as platelet-rich plasma (PRP) and bone marrow concentrate (BMC), to improve tissue repair. We hypothesized that oral losartan (a TGF-β1 blocker with anti-fibrotic properties) could decrease TGF-β1 levels in leukocyte-poor PRP (LP-PRP) and fibrocytes in BMC. Ten rabbits were randomized into two groups (N = 5/group): osteochondral defect + microfracture (control, group 1) and osteochondral defect + microfracture + losartan (losartan, group 2). For group 2, a dose of 10mg/kg/day of losartan was administrated orally for 12 weeks post-operatively. After 12 weeks, whole blood (WB) and bone marrow aspirate (BMA) samples were collected to process LP-PRP and BMC. TGF-β1 concentrations were measured in WB and LP-PRP with multiplex immunoassay. BMC cell populations were analyzed by flow cytometry with CD31, CD44, CD45, CD34, CD146 and CD90 antibodies. There was no significant difference in TGF-β1 levels between the losartan and control group in WB or LP-PRP. In BMC, the percentage of CD31+ cells (endothelial cells) in the losartan group was significantly higher than the control group (p = 0.008), while the percentage of CD45+ cells (hematopoietic cells-fibrocytes) in the losartan group was significantly lower than the control group (p = 0.03).

A novel approach to radiographic detection of bucco-palatal/lingual dilacerations: A preliminary study with ImageJ

2021 ◽  
pp. 095441192110307
Author(s):  
Samed Satir ◽  
Muhammed Hilmi Buyukcavus ◽  
Kaan Orhan
Keyword(s):  
Longitudinal Axis ◽  
Control Group ◽  
Single Root ◽  
Novel Approach ◽  
Significant Difference ◽  
Spectrum Feature ◽  
Group 2 ◽  
Imagej Software ◽  
And Control ◽  
Control Group Group

The purpose of our study is to determine whether bucco-palatal/lingual (BPL) root dilacerations (RD), especially in single root teeth, can be determined using the ImageJ program through only one periapical radiography. Extracted teeth without any RD ( n = 8) were determined as the control group (Group 1) and with RD in apical 1/3 part at least 20° with the longitudinal axis in the BPL direction ( n = 8) as the study group (Group 2). With the help of a simple holder system prepared, digital periapical radiographs of all teeth were taken in an anteroposterior position. Histogram analysis of all periapical radiographs was performed using the spectrum feature of ImageJ software. It was aimed to make a dilaceration analysis by comparing the groups using mean, standard deviation, minimum, maximum, and bin width values. As a result of the Mann-Whitney U test, all mean and maximum values showed a statistically significant difference between the study and control groups ( p < 0.05). This pilot study revealed that the ImageJ software can be used to diagnose BPL dilaceration in the apical 1/3 part of the root. It is important for dentists and patients that it can contribute to limiting the radiation dose to which patients will be exposed.

scholarly journals What is the impact of methotrexate on liver in patients with juvenile idiopathic arthritis? Results of liver SWE performed in a single centre

2021 ◽  
Author(s):  
Vildan Güngörer ◽  
Mehmet Öztürk ◽  
Mustafa Yasir Özlü ◽  
Şükrü Arslan
Keyword(s):  
Juvenile Idiopathic Arthritis ◽  
Shear Wave Elastography ◽  
Term Therapy ◽  
Control Group ◽  
Significant Difference ◽  
Group 2 ◽  
Long Term Therapy ◽  
And Control ◽  
The Impact

ABSTRACT Objectives Long-term therapy with low-dose methotrexate (MTX) is widely used in treatment of rheumatic diseases, in children. The purpose of this study was to evaluate liver elasticity in patients with juvenile idiopathic arthritis (JIA) who received MTX and compare the results with control group. Methods Liver elasticity was evaluated with shear wave elastography (SWE) technique in 25 patients aged 3–17 years who were followed up with JIA and received MTX and compared with 25 healthy controls of the same age and weight. Factors that had an effect on liver elasticity were examined. Results The mean SWE value of patients was 2.64 ± 2.13 m/s and 24.10 ± 18.50 kPa, whereas 1.83 ± 0.16 m/s and 10.09 ± 1.83 kPa in control group. There was a significant difference in liver elasticity in the patient and control groups. When the patients were evaluated as Group 1 (&lt; 1000 mg) and Group 2 (≥ 1000 mg) according to the cumulative MTX dose, no significant difference was obtained. There was positive correlation between liver elasticity and weekly MTX dose and age. Conclusions Our study revealed that liver elasticity significantly decreased in patients who received MTX when compared with the control group. The elastography technique will be understood better over time and used safely in many areas.

scholarly journals Comparison of the results of percutaneous nephrolithotomy in different age groups

2018 ◽  
Vol 5 (12) ◽  
pp. 3888
Author(s):  
Ali Borekoglu ◽  
Ibrahim Atilla Aridogan ◽  
Mutlu Deger ◽  
Onur Karsli ◽  
Volkan Izol
Keyword(s):  
Blood Transfusion ◽  
Percutaneous Nephrolithotomy ◽  
Preoperative Evaluation ◽  
Control Group ◽  
Perioperative Bleeding ◽  
Significant Difference ◽  
The Mean ◽  
Group 2 ◽  
And Control ◽  
Group 1

Background: Evaluation of feasibility, safety and effectiveness of percutaneous nephrolithotomy (PCNL) in different age groups.Methods: Between July 1997-October 2012, 233 patients who were 65 years old and older were included in this study. These patients were divided into two age subgroups used in gerontology research. Group 1 was defined as patients 65-74 years old, Group 2 was older than 74 years old. Data from patient records, including demographic characteristics, preoperative evaluation, operative details, and complications were retrospectively analyzed and compared with control group data.Results: The mean age of 233 patients was 69.7±4.6 years. The mean operative times for Group 1, group 2 and the control group were 76.2±47.3mins, 92.9±47.6mins, 77±44mins, respectively and there was no statistically significant difference between groups 1 and 2 and the control group (p>0.05). Twenty-eight of the 233 patients (12%) needed blood transfusion due to perioperative bleeding. The transfusion rates of groups 1 and 2 were 11.3% and 16.7%, respectively. There was no statistically significant difference between the study groups and control group for blood transfusion rates (p>0.05). There was no statistically significant difference in complications between the study and control groups (p>0.05).Conclusions: In geriatric patients, stone-free rates, transfusion rates and other operation parameters are similar to younger populations when experienced surgeons perform PCNL. Despite comorbidities and decreased body reserve, PCNL can be performed without a significant increase in complications in different ages.

Evaluation of oxidant and antioxidant status in infants with hyperbilirubinemia and kernicterus

2011 ◽  
Vol 30 (11) ◽  
pp. 1751-1760 ◽  
Author(s):  
Murat Doğan ◽  
Erdal Peker ◽  
Ercan Kirimi ◽  
Ertan Sal ◽  
Sinan Akbayram ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Status ◽  
Sulfhydryl Group ◽  
Stress Index ◽  
Control Group ◽  
Significant Difference ◽  
Group 2 ◽  
And Control ◽  
And Oxidative Stress ◽  
Group 1

Objective: The objective of the present study was to determine oxidant and antioxidant status in infants with hyperbilirubinemia and/or kernicterus and to find whether there is a relationship between bilirubin level and oxidant/antioxidant status. Patients: The study includes 69 full-term newborns (neonates with hyperbilirubinemia needing phototherapy [Group 1, n = 36] and neonates with kernicterus [Group 2, n = 33]) and 25 age-matched healthy newborn. Results: Plasma total antioxidant capacity (TAC) and serum total oxidant status (TOS) were significantly higher in Groups 1 and 2 than the control group. There was a significant difference between Group 1 and control cases for malondialdehyde (MDA; p < 0.001). Total free sulfhydryl group (TTHI) values were significantly elevated in Group 1 compared to Group 2 and control cases. Correlation analysis showed that the correlation between total bilirubin (TB) and TAC, TOS, MDA and oxidative stress index may be expressed by a quadratic curve. After phototherapy, a statistically significant increase in nitrite level was observed. Conclusion: We demonstrated that the relationship between serum TB and antioxidants and oxidative stress could be expressed by a quadratic correlation curve.

scholarly journals Pengaruh Teknik Vulva Hygiene terhadap Jumlah Kuman Vulva pada Ibu Nifas di BPM Kota Bandar Lampung

2017 ◽  
Vol 8 (3) ◽  
pp. 465
Author(s):  
Nelly Indrasari ◽  
Purwati Purwati
Keyword(s):  
Degrees Of Freedom ◽  
Laboratory Data ◽  
Research Unit ◽  
Control Group ◽  
Chi Square ◽  
Significance Level ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
And Control

<p>The case of maternal mortality in Bandar Lampung city in 2013 is 19 cases, in 2014 there are 7 cases and all died during childbirth and there are 19 cases of maternal death by 2015. (Profile of Lampung Province Health Office 2014). Research Objectives to Detect the Influence of Vulva Hygiene Technique on Number of Vulva Germs In Nifas Mother In BPM Bandar Lampung City Year 2016. This research method using quasi-experiment design. This study compares between treated groups and control groups. Treatment group 1 was treated with vulvar hygiene by using 1 cotton, group 2 using 3 kinds of cotton, group 3 using 5 cotton and control group without treatment. Population in this research is mother Nanyang checking his health at BPM in Bandar Lampung city at the time of research. The sample of respondent's research is 120. Data collection with Teradata is done as much as 1 time then done culture and examination in a local laboratory. Data were processed and analyzed by independent T-test. The result showed that the average number of germs was 2277,37 germs (95% CI 7140,59-38402,16) with standard deviation 65553,94 germs. The lowest number of germs and pathogen bacteria still 0.35% of respondents on the vulva hygiene of a cotton with a duration of 26 days. The result of analysis with cruciate Wallis test is 3,498. Looking at the statistics of the table by looking at the Chi-square table, for df (degrees of freedom)=3 and the significance level (α) = 5%, then obtained statist table 2.32. Because of the count statistic (2.32&gt; 0.321), then Ho accepted, or no significant difference (significant). The research unit can utilize and apply vulva hygiene technique with antiseptic, so it can prevent the occurrence of infection in the postpartum mother.</p>

scholarly journals High-performance inulin and oligofructose prebiotics increase the intestinal absorption of iron in rats with iron deficiency anaemia during the growth phase

2011 ◽  
Vol 108 (6) ◽  
pp. 1008-1016 ◽  
Author(s):  
Karine de Cássia Freitas ◽  
Olga Maria Silvério Amancio ◽  
Mauro Batista de Morais
Keyword(s):  
Intestinal Absorption ◽  
High Frequency ◽  
High Performance ◽  
Fe Deficiency ◽  
Control Group ◽  
Control Groups ◽  
Deficiency Anaemia ◽  
Significant Difference ◽  
Group 2 ◽  
And Control

Considering the high frequency of anaemia due to Fe deficiency, it is important to evaluate the effects of prebiotics on the absorption of Fe. The aim of the present study was to evaluate the effects of high-performance (HP) inulin, oligofructose and synergy1 during recovery from anaemia in rats through the intestinal absorption of Fe, food intake, body growth, caecal pH and weight of the intestine. Wistar rats (n 47) were fed with rations of AIN93-G with no Fe to induce Fe deficiency anaemia. At 36 d of life, anaemic rats were divided into four groups: (1) the HP inulin group; (2) the synergy1 group; and (3) the oligofructose group, all with 100 g of the respective prebiotic per kg of ration; and (4) a control group, in which the prebiotic was replaced by maize starch. Then, 25 mg of elemental Fe/kg of ration was added to all rations to allow recovery from anaemia. The final values of Hb in the HP inulin, synergy1, oligofructose and control groups were, respectively: 98 (94–99); 83 (81–92); 100 (90–114); 77 (72–81) g/l, with a statistically significant difference (P ≤ 0·001) between the oligofructose and control groups and the HP inulin and control groups. The four groups had an increase in weight and body length and had similar consumption of rations. The intestinal weight and caecal pH were significantly different between the groups that consumed prebiotics and the control group. HP inulin and oligofructose increased the intestinal absorption of Fe in rats.

scholarly journals Acute toxicity of the water-soluble fraction of diesel in Prochilodus vimboidesKner (Characiformes: Prochilodontidae)

2013 ◽  
Vol 11 (1) ◽  
pp. 193-198 ◽  
Author(s):  
Celina Alcoforado Santos ◽  
Dominik Lenz ◽  
Geisamanda Pedrini Brandão ◽  
Adriana Regina Chippari-Gomes ◽  
Levy Carvalho Gomes
Keyword(s):  
Soluble Fraction ◽  
Micronucleus Test ◽  
Exposure Period ◽  
Diesel Oil ◽  
Water Soluble ◽  
Control Fish ◽  
Control Group ◽  
Water Soluble Fraction ◽  
Significant Difference ◽  
And Control

Diesel oil can be a source of contamination in aquatic environments, mainly as a result of spills. The effects of the water-soluble fraction of diesel (WSF) on Prochilodus vimboideswere assessed. Fish were exposed to three different WSF dilutions for up to 96 h and were compared to a control group. Damages in the fragments of DNA were analyzed using the Comet assay. The presence of erytrocyts abnormalities was assessed by micronucleus test. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity and the accumulation of copper in gills were also analyzed. Fish exposed for 96 h had higher rates of damage than those exposed for 24 h. There was no significant difference regarding the presence of micronuclei between exposed and control fish and between 24-h and 96-h exposures. For AST, no significant difference was observed between samples collected at the two exposure times. Fish exposed to a 1:100 dilution of WSF showed higher activity of the enzyme ALT than the control fish after a 24-h exposure period. There was no bioaccumulation of copper in the gills. We conclude that the genotoxic effects of WSF in the cells are more evident in P. vimboides during an acute exposure.

Pneumogram Recordings in Infants Resuscitated for Apnea of Infancy

PEDIATRICS ◽  
1989 ◽  
Vol 83 (3) ◽  
pp. 364-368
Author(s):  
Joseph Oren ◽  
Dorothy H. Kelly ◽  
Daniel C. Shannon
Keyword(s):  
Sudden Infant Death ◽  
Control Group ◽  
Sudden Infant ◽  
Increased Risk ◽  
Significant Difference ◽  
Monitoring Group ◽  
Subsequent Event ◽  
Group 3 ◽  
Group 2 ◽  
And Control

Infants who sustained a spell of apnea of infancy during which they were resuscitated have been shown to be at increased risk for sudden infant death syndrome. To determine the value of the pneumogram as a predictor of outcome in this population, the first pneumogram obtained of 51 such infants was analyzed. The infants were grouped on the basis of subsequent outcome during a course of monitoring: group 1—infants who died during a subsequent event; group 2—infants who received resuscitation or vigorous stimulation to terminate a subsequent spell; group 3—infants who survived and did not have a significant subsequent episode. The results for these infants were compared with those of a control group matched for age and sex. A detailed, blinded computer analysis revealed no significant difference in the results of the pneumogram analysis between the three groups. It did not identify the infants in whom apneic spells necessitating resuscitation subsequently developed or those who died. However, when compared with the control group, infants with apnea of infancy had significantly higher mean respiratory rates, heart rates, and tachycardia indexes. It is concluded that, although the first pneumogram does not predict the risk of an adverse outcome in a population of infants with severe apnea of infancy, it does reveal subtle cardiorespiratory differences between study and control infants.

scholarly journals Integrating Declarative and Procedural Knowledge on Grammar through E- Portfolio Assessment

2020 ◽  
Vol 6 (1) ◽  
pp. 37-54
Author(s):  
Rukminingsih Rukminingsih
Keyword(s):  
Data Analysis ◽  
Procedural Knowledge ◽  
Portfolio Assessment ◽  
Sampling Technique ◽  
Control Group ◽  
Significant Difference ◽  
Quantitative Data Analysis ◽  
Group 2 ◽  
And Control ◽  
Experimental Group

This study aims to reveal (1) whether there is significance different students’ achievement who are taught grammar through  integrating declarative and procedural knowledge by implementing E-Portfolio assessment  and (2) the process of  integrating declarative and procedural knowledge by implementing E-Portfolio assessment. It employed mixed method of concurrent design.The population of this research was 120 students of grammar class in English Department of STKIP PGRI Jombang. The number of sample was 40 students for experimental group and 40 students for control group by employing cluster random sampling technique. The data was collected through test and observation and it applied ANCOVA for quantitative data analysis and data display, data reduction and drawing conclusion for qualitative data analysis. The finding showed that (1) there is significant difference on students’ grammar understanding between experimental group and control group. (2) E portfolio through students’ blogs gives opportunity to students to engage their efforts, progress, and achievements in given areas and the strategy has the additional benefit of enabling students to publish and share ideasor tasks easily involving authentic audience or their classmates.It implied that assessing by using Electronic Portfolio is positively effective to be implemented in integrating both declarative and procedural knowledge in Grammar course.

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