LncRNA MIAT regulated by selenium and T-2 toxin increases NF-κB-p65 activation, promoting the progress of Kashin-Beck Disease

LncRNA myocardial infarction associated transcript (MIAT) has been shown to be involved in osteoarthritis (OA), but its role in Kashin-Beck Disease (KBD) has rarely been reported. In this study, rats were administered with low selenium and/or T-2 toxin for 4 weeks to establish a KBD animal model. The serum selenium level, TNF-α and IL-1β contents, phosphorylated p65 (p-p65) and MIAT expression were increased in each intervention group. Next, we isolated the primary epiphyseal chondrocytes, and found that selenium treatment reversed the effects of T-2 toxin on chondrocyte injury, p-p65 and MIAT expression. In addition, MIAT overexpression or T-2 toxin treatment led to increased cell death, apoptosis, inflammation, NF-κB-p65 pathway activation and MIAT expression, which was rescued by selenium treatment or MIAT siRNA transfection. Our results suggested that lncRNA MIAT regulated by selenium and T-2 toxin increased the activation of NF-κB-p65, thus being involved in the progress of KBD. [Formula: see text]

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Serum Selenium Level and 10-Year Survival after Melanoma

Biomedicines ◽

10.3390/biomedicines9080991 ◽

2021 ◽

Vol 9 (8) ◽

pp. 991

Author(s):

Emilia Rogoża-Janiszewska ◽

Karolina Malińska ◽

Piotr Baszuk ◽

Wojciech Marciniak ◽

Róża Derkacz ◽

...

Keyword(s):

Selenium Level ◽

Serum Selenium ◽

Lower Survival Rate ◽

Treatment Regimens ◽

Melanoma Diagnosis ◽

Serum Selenium Levels ◽

Melanoma Prognosis ◽

Level I ◽

Low Selenium ◽

Serum Selenium Level

Melanoma is one of the most aggressive human malignancies. The determination of prognostic biomarkers is important for the early detection of recurrence and for the enrollment of the patients into different treatment regimens. Herein, we report the 10-year survival of 375 melanoma patients depending on their serum selenium levels. The study group was followed up from the date of melanoma diagnosis until death or 2020. Patients were assigned to one of four categories, in accordance with the increasing selenium level (I–IV quartiles). The subgroup with low selenium levels had a significant lower survival rate in relation to patients with high selenium levels, HR = 8.42; p = 0.005 and HR = 5.83; p = 0.02, for uni- and multivariable models, respectively. In the univariable analysis, we also confirmed the association between Breslow thickness, Clark classification and age at melanoma prognosis. In conclusion, a low serum selenium level was associated with an increased mortality rate in the 10 years following melanoma diagnosis. Future studies in other geographic regions with low soil selenium levels should be conducted to confirm our findings.

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Serum selenium and risk of prostate cancer in Japanese men

Journal of Clinical Oncology ◽

10.1200/jco.2009.27.15_suppl.e16166 ◽

2009 ◽

Vol 27 (15_suppl) ◽

pp. e16166-e16166

Author(s):

M. Nozawa ◽

M. Yoshida ◽

Y. Saito ◽

M. Nakagawa ◽

T. Ozeki ◽

...

Keyword(s):

Prostate Cancer ◽

Prostate Biopsy ◽

Selenium Level ◽

Control Group ◽

Case Group ◽

Serum Selenium ◽

Inverse Association ◽

Serum Selenium Levels ◽

Low Selenium ◽

Serum Selenium Level

e16166 Background: Epidemiologic studies suggest that low selenium levels are associated with an increased incidence of prostate cancer, although results are conflicting. We examined the association between serum selenium levels and risk of prostate cancer in men who received a prostate biopsy in our hospital. Methods: Our prospective study included 82 patients who received a prostate biopsy based on elevated PSA levels over 4.0ng/ml. Serum from each patient was sampled to determine the selenium level at the time of biopsy. Of these, 47 patients were diagnosed with prostate cancer (case group) and no malignant lesions in the biopsy specimen were detected in the remains, 35 patients (control group). Results: The mean serum selenium levels in the case and control group were 120.4 (SD, 14.4) ng/mL and 118.5 (SD, 16.1) ng/mL, respectively (p = 0.588). Serum selenium levels at biopsy were not associated with risk of prostate cancer diagnosis. In the case group, the mean serum selenium levels in patients diagnosed with 6 or less in Gleason score and 7 or more were 121.0 (SD, 9.9) ng/mL (n = 14) and 120.1 (SD, 16.1) ng/mL (n = 33), respectively (p = 0.851). Serum selenium levels at biopsy were not associated with Gleason score in the case group. An inverse association between serum selenium and PSA levels at biopsy was observed (p = 0.030). No correlation was observed between serum selenium level and age at biopsy. Conclusions: From this study, serum selenium level cannot predict the result of prostate biopsy. The inverse association between serum selenium and PSA levels may suggest that low selenium levels are associated with an increased incidence of prostate cancer. No significant financial relationships to disclose.

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Serum selenium level in acute myocardial infarction

The Egyptian Journal of Internal Medicine ◽

10.4103/ejim.ejim_63_17 ◽

2018 ◽

Vol 30 (1) ◽

pp. 28

Author(s):

MaiA Fathi ◽

AbdallahI Ali Kelani ◽

HananS El-Deen Mohammed ◽

MonaM Soliman ◽

Marwa Sayed ◽

...

Keyword(s):

Myocardial Infarction ◽

Acute Myocardial Infarction ◽

Selenium Level ◽

Serum Selenium ◽

Serum Selenium Level

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Direct cost of treatment in patients with acute ST-elevation myocardial infarction in Vietnam

MedPharmRes ◽

10.32895/ump.mpr.3.3.2 ◽

2019 ◽

Vol 3 (3) ◽

pp. 7-11

Author(s):

Sy Van Hoang ◽

Tuan Thanh Tran ◽

Kha Minh Nguyen

Keyword(s):

Myocardial Infarction ◽

Direct Cost ◽

Treatment Cost ◽

Financial Burden ◽

Intervention Group ◽

St Elevation Myocardial Infarction ◽

Related Factors ◽

Female Ratio ◽

Conservative Group ◽

St Elevation

Background: Acute myocardial infarction has become a serious financial burden for patients, healthcare system, and society. It is therefore necessary to assess treatment cost of myocardial infarction that had been conducted in many countries in the world and still not fully analysed in Vietnam. Thus, we sought to describe acute ST-elevation myocardial infarction treatment cost and analyse related factors to acute ST-elevation myocardial infarction treatment cost. Methods and Materials: A retrospective cross-sectional study. Patients who was diagnosed by ST-elevation myocardial infarction at Cho Ray Hospital from June 2018 to February 2019, satisfied inclusion and exclusion criteria. Results: We collected 130 patients with acute ST-elevation myocardial infarction with male: female ratio of 3:1, at average age of mean ± Standard deviation (SD) = 62.9 ± 12.6. The length of stay in hospital was mean ± SD = 7.1 ± 3.3 days and the median direct cost of MI was 68,902,500 VND (interquartile range (IQR): 5,737,200 – 104,266,000 VND). The average total cost of acute ST-elevation myocardial infarction in the percutaneous coronary intervention group was more than 16 times as the conservative group. The treatment strategies and hospital complications were major factors that affected treatment cost. Conclusion: The median direct cost of acute ST-elevation myocardial infarction was accounted for 68,902,500 VND. Complications directly affected costs.

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Protective effect of a new heterocyclic compound on acute tracheobronchitis via reducing IL-6 and TNF-α content and PKA-NF-κB pathway activation

Main Group Chemistry ◽

10.3233/mgc-210006 ◽

2021 ◽

Vol 20 (1) ◽

pp. 41-48

Author(s):

Ai-Ping Han ◽

Li Li

Keyword(s):

Protective Effect ◽

Heterocyclic Compound ◽

Biological Study ◽

Elisa Assay ◽

Mice Model ◽

X Ray ◽

X Ray Crystallography ◽

Pathway Activation ◽

Tnf Α ◽

Amino Benzoic Acid

The new heterocyclic compound 4-methyl-3-((4-(pyridin-3-yl) pyrimidin-2-yl) amino) benzoic acid (1) designed utilizing methyl 3-amino-4-methylbenzoate (2) as a starting material was successfully fabricated and eventually characterized utilizing single crystal X-ray crystallography, 1H NMR and IR. In biological study, to evaluate the protective effect of compound on acute tracheobronchitis ICR mice model, the ELISA assay was performed to determine the level of inflammatory mediators IL-6 and TNF-α in serum. Then, the western blot was performed to determine the activation of PKA-NF-κB pathway in tissues.

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Cardioprotective effects of Ginsenoside compound-Mc1 and Dendrobium Nobile Lindl against myocardial infarction in an aged rat model: Involvement of TLR4/NF-κB signaling pathway

European Journal of Inflammation ◽

10.1177/20587392211000577 ◽

2021 ◽

Vol 19 ◽

pp. 205873922110005

Author(s):

Yongle Sun ◽

Jing Geng ◽

Deyu Wang

Keyword(s):

Myocardial Infarction ◽

Combination Therapy ◽

Infarct Size ◽

Signaling Pathway ◽

Left Ventricular ◽

Male Rats ◽

Dendrobium Nobile ◽

Tnf Α ◽

Cardioprotective Effects ◽

Dendrobium Nobile Lindl

Aging is the crucial co-morbidity that prevents the full cardioprotection against myocardial ischemia/reperfusion (I/R) injury. Combination therapy as a promising strategy may overcome this clinical problem. This study aimed to investigate the cardioprotective effects of Ginsenoside compound-Mc1 (GMc1) and Dendrobium Nobile Lindl (DNL) in myocardial I/R injury and explore the involvement of the TLR4/NF-κB signaling pathway in aged rats. In vivo I/R injury and myocardial infarction was established by temporary coronary ligation in 22–24 months’ old Sprague Dawley male rats. GMc1 (10 mg/kg) and DNL (80 mg/kg) were administered intraperitoneally for 4 weeks and orally for 14 days, respectively, before I/R injury. Infarct size was measured through triphenyl-tetrazolium-chloride staining. ELISA assay was conducted to quantify the levels of cardiotroponin, and myocardial content of TNF-α and glutathione. Western blotting was employed to detect the expression of TLR4/MyD88/NF-κB proteins. GMc1 and DNL significantly reduced the infarct size to a similar extent ( p < 0.05) but their combined effect was greater than individual ones ( p < 0.01). Combination therapy significantly restored the left ventricular end-diastolic and developed pressures at the end of reperfusion as compared with the untreated group ( p < 0.01). Although the GMc1 and DNL reduced the levels of inflammatory cytokine TNF-α and increased the contents of antioxidant glutathione significantly, their individual effects on the reduction of protein expression of TLR4/MyD88/NF-κB pathway were not consistent. However, their combination could significantly reduce all parameters of this inflammatory pathway as compared to untreated I/R rats ( p < 0.001). Therefore, the combined treatment with GMc1 and DNL increased the potency of each intervention in protecting the aged hearts against I/R injury. Reduction in the activity of the TLR4/MyD88/NF-κB signaling pathway and subsequent modulation of the activity of inflammatory cytokines and endogenous antioxidants play an important role in this cardioprotection.

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A randomized trial of the effect of peer education on the sexual quality of life in patients with myocardial infarction

Journal of Complementary and Integrative Medicine ◽

10.1515/jcim-2019-0204 ◽

2020 ◽

Vol 17 (3) ◽

Author(s):

Ali Abbasi ◽

Hossein Ebrahimi ◽

Hossein Bagheri ◽

Mohammad Hasan Basirinezhad ◽

Seyedmohammad Mirhosseini ◽

...

Keyword(s):

Quality Of Life ◽

Myocardial Infarction ◽

Health Care Professionals ◽

Peer Education ◽

Intervention Group ◽

Control Group ◽

Controlled Clinical Trial ◽

Sexual Quality Of Life ◽

Significant Difference

AbstractBackgroundOne of the factors associated with readmission is inappropriate sexual activity after myocardial infarction (MI). This study aimed to assess the effect of peer education on the sexual quality of life (SQOL) in patients with MI.MethodsIn this randomized controlled clinical trial, 70 patients with MI meeting the inclusion criteria were assigned to experimental or control groups using block randomization. Peer education for the intervention group was provided on the third day after MI. Education sessions lasted from 90 to 120 minutes. Data were collected using demographic, sexual quality of life-female (SQOL-F), and sexual quality of life-male (SQOL-M) questionnaires during the fourth week after MI.ResultsThere was no significant difference between the two groups before the intervention in terms of demographic and SQOL. The mean of SQOL in the peer education group was significantly higher than the control group at the 4-week follow-up.ConclusionsAccording to the results, using peer education is recommended for the sexual health care professionals.

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