Antisickling effect of chrysin is associated with modulation of oxygenated and deoxygenated haemglobin via alteration of functional chemistry and metabolic pathways of human sickle erythrocytes

2021 ◽  
pp. 096032712110255
Author(s):  
HC Nwankwo ◽  
AA Idowu ◽  
A Muhammad ◽  
AD Waziri ◽  
YS Abubakar ◽  
...  
Keyword(s):  
Metabolic Pathways ◽  
Osmotic Fragility ◽  
Developing Nations ◽  
Control Group ◽  
Cell Disease ◽  
Sickle Erythrocytes ◽  
Before And After ◽  
And Shifts ◽  
High Level ◽  
2,3 Dpg

Sickle cell disease (SCD) treatment and management remain a challenging puzzle especially among developing Nations. Chrysin’s sickling-suppressive properties in human sickle (SS) erythrocytes in addition to its effect on AA-genotype erythrocytes were evaluated. Sickling was induced (76%) with 2% sodium metabisulphite at 3 h. Chrysin prevented (81.19%) the sickling and reversed same (84.63%) with strong IC50s (0.0257 µM and 0.00275 µM, respectively). The levels of oxygenated haemoglobin in the two groups (before and after induction approaches) were similar but significantly (P < 0.05) higher than that of SS erythrocytes (the ‘induced’ control), with chrysin-treated AA-genotype showing no effects relative to the untreated. The level of deoxygenated haemoglobin in the ‘induced’ control group was significantly (P < 0.05) higher than those of the chrysin-treated SS erythrocytes. Normal and chrysin-untreated erythrocytes (AA-untreated) were significantly more resistant to osmotic fragility than the SS-untreated. However, treatment with chrysin significantly reduced the osmotic fragility of the cells relative to the untreated cells. Furthermore, chrysin treatment significantly lowers the high level of 2,3-diphosphoglycerate (2,3-DPG) observed in the sickle erythrocytes, with no effects on AA-genotype erythrocytes. Based on functional chemistry, chrysin treatment alters the functional groups in favour of its antisickling effects judging from the observed bends and shifts. From metabolomics analysis, it was observed that chrysin treatment favors fatty acid alkyl monoesters (FAMEs) production with concomitant shutting down-effects on selenocompound metabolism. Thus, sickling-suppressive effects of chrysin could potentially be associated with modulation of oxygenated and deoxygenated haemglobin via alteration of human sickle erythrocyte’s functional chemistry and metabolic pathways implicated in SCD crisis.

scholarly journals Benefit of a pharmacist-led intervention for medication management of renal transplant patients: a controlled before-and-after study

2021 ◽  
Vol 12 ◽  
pp. 204062232110052
Author(s):  
Jeremy Chambord ◽  
Lionel Couzi ◽  
Pierre Merville ◽  
Karine Moreau ◽  
Fabien Xuereb ◽  
...  
Keyword(s):  
Medication Adherence ◽  
Immunosuppressive Therapy ◽  
Medication Management ◽  
Intervention Group ◽  
Knowledge Score ◽  
Control Group ◽  
Patient Knowledge ◽  
Transplant Patients ◽  
Before And After ◽  
High Level

Aims: To assess the effect of a pharmacist-led intervention, using Barrows cards method, during the first year after renal transplantation, on patient knowledge about their treatment, medication adherence and exposure to treatment in a French cohort. Methods: We conducted a before-and-after comparative study between two groups of patients: those who benefited from a complementary pharmacist-led intervention [intervention group (IG), n = 44] versus those who did not [control group (CG), n = 48]. The pharmacist-led intervention consisted of a behavioral and educational interview at the first visit (visit 1). The intervention was assessed 4 months later at the second visit (visit 2), using the following endpoints: treatment knowledge, medication adherence [proportion of days covered (PDC) by immunosuppressive therapy] and tacrolimus exposure. Results: At visit 2, IG patients achieved a significantly higher knowledge score than CG patients (83.3% versus 72.2%, p = 0.001). We did not find any differences in treatment exposure or medication adherence; however, the intervention tended to reduce the proportion of non-adherent patients with low knowledge scores. Using the PDC by immunosuppressive therapy, we identified 10 non-adherent patients (10.9%) at visit 1 and six at visit 2. Conclusions: Our intervention showed a positive effect on patient knowledge about their treatment. However, our results did not show any improvement in overall medication adherence, which was likely to be because of the initially high level of adherence in our study population. Nevertheless, the intervention appears to have improved adherence in non-adherent patients with low knowledge scores.

The relation to pregnancy, the child, motherhood of women in IVF situation

2015 ◽  
Vol 6 (4) ◽  
pp. 97-104
Author(s):  
Arina Yurevna Malenova ◽  
Irina Gennadevna Kytkova
Keyword(s):  
Family Relations ◽  
First Trimester ◽  
Family Education ◽  
In Vitro Fertilisation ◽  
Control Group ◽  
The Family ◽  
Before And After ◽  
Group 2 ◽  
High Level

Research objective - studying of features of the relation to pregnancy, the child, motherhood of women in IVF situation. Selection: 100 married pregnant women aged from 28 till 42 years (the first pregnancy of the first trimester, complications in the anamnesis isn't present) representing two groups on 50 people: 1) after artificial insemination (empirical group); 2) in a situation natural pregnancy (control group). The leading motives of pregnancy, types of the attitude towards themselves, pregnancies, to the child, people around, the prevailing installations in the sphere of the family relations, features of representation of future mothers about themselves and "the ideal parent" are defined by testing. Distinctions in all respects with women from control group are found. It is established that in vitro fertilisation the high level of readiness for motherhood according to its motivational characteristics is observed. Prevalence of constructive motives of pregnancy against concern in the health and aspirations to meet social expectations is revealed. The leading types of a gestational dominant are optimum and euphoric, the hypertrophied positive emotional background of pregnancy is observed. In the future of a bike probability the dependent relations with the child, preference of the sponsoring or authoritative style of family education. Revaluation of own parental qualities when comparing with image of ideal mother is observed. Results allow to carry women to the group of risk demanding psychological maintenance before and after the childbirth.

scholarly journals Why do I have to drive now? Post Hoc Explanations of Take-Over Requests

2017 ◽  
Author(s):  
Moritz Körber ◽  
Lorenz Prasch ◽  
Klaus Bengler
Keyword(s):  
Interface Design ◽  
Control Group ◽  
Automated Vehicles ◽  
Automated Driving ◽  
Trust In Automation ◽  
Before And After ◽  
High Level ◽  
Post Hoc ◽  
Experimental Group

Objective: It was investigated whether providing an explanation for a take-over request in automated driving influences trust in automation and acceptance.Background: Take-over requests will be recurring events in conditionally automated driving which could undermine trust as well as acceptance and, therefore, the successful introduction of automated vehicles.Method: Forty participants were equally assigned to either an experimental group provided with an explanation of the reason for a take-over request or a control group without explanations. In a simulator drive, both groups experienced three take-over scenarios that varied in the obviousness of their causation. Participants rated their acceptance before and after the drive and rated their trust before and after each take-over situation. Results: All participants rated acceptance on the same high level before and after the drive, independent of the condition. Control group’s trust ratings remained unchanged by take-over requests in all situations, but the experimental group showed decreased trust after experiencing a take-over caused by roadworks. Participants provided with explanation felt stronger that they had understood the system and the reasons for the take-overs.Conclusion: A take-over request did not lower trust or acceptance. Providing an explanation for a take-over request had no impact on trust or acceptance, but increased the perceived understanding of the system.Application: The results provide insights into users’ perception of automated vehicles, take-over situations and a fundament for future interface design for automated vehicles.

scholarly journals The Effect of Using Music Classic (Mozart) towards Student Anxiety Before The Exams

2020 ◽  
Vol 5 (1) ◽  
pp. 47-56
Author(s):  
Risa Yuspitasari ◽  
Rahmawati ◽  
Raudah Zaimah Dalimunthe
Keyword(s):  
Experimental Method ◽  
Sampling Technique ◽  
Control Group ◽  
Student Anxiety ◽  
Purposive Sampling ◽  
High Category ◽  
Before And After ◽  
Post Test ◽  
High Level ◽  
Experimental Group

This research aimed to discover the level of anxiety in students before the exam and the effect of music classic (Mozart) to reduce such anxiety. The study was conducted at SMP Negeri 1 Ciruas with students with a high level of anxiety, which has been revealed from the results of the anxiety scale questionnaire using a purposive sampling technique with a total of 68 respondents. This research was used with an experimental method consisting of an experimental group and a control group. From the treatment, the results showed pre-test 12.55 in the high category and post-test 7.852 in the medium category. This shows changes in the results before and after the treatment is given. It can be concluded that the effect of the use of music (Mozart) can reduce student anxiety before the test.

A pilot study of cognitive therapy in bipolar disorders

2001 ◽  
Vol 31 (3) ◽  
pp. 459-467 ◽  
Author(s):  
J. SCOTT ◽  
A. GARLAND ◽  
S. MOORHEAD
Keyword(s):  
Cognitive Therapy ◽  
Psychiatric Treatment ◽  
Internal State ◽  
Bipolar Depression ◽  
Bipolar Disorders ◽  
Waiting List ◽  
Control Group ◽  
Hospitalization Rates ◽  
Before And After ◽  
High Level

Background. The efficacy and effectiveness of cognitive therapy (CT) is well established for unipolar disorders, but little is known about its utility in bipolar disorders. This study aimed to explore the feasibility and efficacy of using CT as an adjunct to usual psychiatric treatment in this patient population.Method. Subjects referred by general adult psychiatrists were assessed by and independent rater and then randomly allocated to immediate CT (N=21) or 6-month waiting-list control, which was then followed by CT (N=21). Observer and self-ratings of symptoms and functioning were undertaken immediately prior to CT, after a 6-month course of CT and a further 6-months later. Data on relapse and hospitalization rates in the 18 months before and after commencing CT were also collected.Results. At 6-month follow-up, subjects allocated to CT showed statistically significantly greater improvements in symptoms and functioning as measured on the Beck Depression Inventory, the Internal State Scale, and the Global Assessment of Functioning than those in the waiting-list control group. In the 29 patients who eventually received CT, relapse rates in the 18 months after commencing CT showed a 60% reduction in comparison with the 18 months prior to commencing CT. Seventy per cent of subjects who commenced therapy viewed CT as highly acceptable.Conclusion. Although the results of this study are encouraging, the use of CT in subjects with bipolar disorders is more complex than in unipolar disorders and requires a high level of therapist expertise. The therapy may prove to be particularly useful in the treatment of bipolar depression.

scholarly journals Using Technology to Enhance the Performance of Intellectually Disabled Students: Mobile Game-based Urdu Learning

2021 ◽  
Vol 4 (1) ◽  
pp. 42-57
Author(s):  
Nida Gul ◽  
Hina Noor ◽  
Farkhunda Rasheed Chaudhary
Keyword(s):  
Intervention Program ◽  
Teaching Method ◽  
Positive Impact ◽  
Learning Goals ◽  
Control Group ◽  
Traditional Teaching ◽  
Before And After ◽  
Quasi Experimental ◽  
Digital Exclusion ◽  
High Level

The notion of using technology for learning is not new. However, the high level of digital exclusion of individuals with intellectual disabilities is well-documented. The purpose of this paper was to investigate the effect of two different pedagogies for students with intellectual disability (ID). The main objective was the comparison of assistive technology and the traditional teaching method for improving Urdu vocabulary. Using a quasi-experimental design, 8 ID students of 10 to 15 yearsof age were selected and divided into control and experimental groups. Their performance was measured before and after a six-week intervention program. The results showed that mobile-based technology improved the experimental group’s performance as compared to the control group. The technology had a positive impact on the learning of Urdu vocabulary by students with ID. The findings suggest that learning the Urdu language through game-based software/programs/utilities can improve students’ learning in the classroom. Teachers can use mobile phone as a learning tool to enhance their learning goals.

Stabilization of Human Erythrocytes for Lyophilization: Recovery of Biochemical Activities and Membrane Filterability.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 3670-3670
Author(s):  
Shang-Zhi Xu ◽  
Arthur P. Bode
Keyword(s):  
Red Blood Cells ◽  
Pore Size ◽  
Blood Cells ◽  
White Blood Cells ◽  
Osmotic Fragility ◽  
Homocysteic Acid ◽  
Human Red Blood Cells ◽  
Before And After ◽  
Pass Through ◽  
2,3 Dpg

Abstract It has much more advantages to preserve human red blood cells (RBC) in freezing and drying form than conventional blood storage, even though it is still under developing. In the present study, we successfully used 0.045% glutaraldehyde(GA) to fix RBCs under appropriate conditions prior to lyophilization and regained the bulk of RBCs membrane filterability. The phlebotomized whole blood was anti-coagulated with CPDA-1 and the plasma was removed by centrifugation. White blood cells were removed using Leucocytes filter (Pall Corporation). 1 x 10 9 cells/ml were separately incubated with 0.03%, 0.045% and 0.05% GA at 37 °C for 10 min; RBCs at concentrations 0.5 x 10 9, 1 x 10 9 and 2 x 10 9 cell/ml were incubated with 0.045% GA at 37 °C for 10 min; Bovine serum albumin (BSA), trehalose, hydroxyethyl starch and dextran were used as protective additive in freezing and drying RBCs, respectively; ethanolamine, glycine, lysine, BSA, glutamic acid and homocysteic acid were used to recover membrane deformability due to GA fixation. Recovery RBCs yield after reconstituion was determined by a cell counter. Fee hemoglobin , glutathione (GSH), 2,3-diphosphoglycerate (2,3-DPG), ATP, and Glucose-6-phosphate dehydrogenase (G-6-PDH) in RBCs were determined before and after lyophilization. Membrane flexibility was assayed by osmotic fragility test. RBCs filterability was determined using positive filter apparatus through 5 micrometer pore size nitrocellulose membrane. Our results suggested that RBCs at 1 x 10 9 cells/ml fixed by 0.045% GA for10 min at pH 7.8 were enough to resist freezing and drying damages and the bulk of RBCs’s membrane filterability were remained. The effective fixation of GA is dependent on GA’s concentration, RBCs concentration, incubation period and pH etc. BSA is the most potential additive in preserving RBCs. More interestingly, the following reagents can recover the reduced RBCs membrane filterability by GA fixation. The potency of recovery is in the order of : 10 mM ethanolamine > 5 mM lysine > 0.5%BSA > 10 mM glutamatic acid > 5 mM homcysteic acid > 2% glycine. After reconstitution, 85 ± 2.3% RBCs yield is achieved, 71 ± 4.1% of these RBCs can freely pass through 5 micrometer pore size filter membrane. Biochemical function indexes as GSH, 2,3-DPG, ATP, and G-6-PDH are saved by 20 ± 1.2%. Lyophilized human red blood cells yields from reconstitution by different treatment. 1x 109 cells/ml fixed at 0.03% glutaraldehyde 44.7 ± 4.1 0.045%glutaraldehyde 84.7 ± 5.8 0.05%glutaraldehyde 88.7 ± 7.6 0.045% glutaraldehyde fix: 0.5x 109 cells/ml 91 ± 4.3 1x 109 cells/ml 84.7 ± 5.8 2 x 109 cells/ml 35.2 ± 2.3 Effects of various treatment on lyophilized RBCs after reconstitution. Group Filterability(%) 1x9 cells/ml fixed by: N = 5. 0.03%glutaraldehyde 88.3 ± 1.2 0.045%glutaraldehyde 73.3 ± 1.8 0.05%glutaraldehyde 44.7 ± 2.8 0.045%glutaraldehyde fixation at: 0.5x109 cells/ml 0 1x109 cells/ml 73.3 ± 1.8 2x109 cells/ml 83.9 ± 4.6

The effect on milk composition of feeding spring grass to cows

1959 ◽  
Vol 26 (2) ◽  
pp. 173-181 ◽  
Author(s):  
R. Waite ◽  
M. E. Castle ◽  
J. N. Watson
Keyword(s):  
Nutrient Intake ◽  
Specific Effect ◽  
Milk Composition ◽  
Soluble Carbohydrates ◽  
Control Group ◽  
Stage Of Lactation ◽  
Milk Protein Content ◽  
Before And After ◽  
High Level ◽  
Β Lactoglobulin

1. An experiment to investigate the seasonal increase in the solids-not-fat content of milk, which usually occurs in Britain when cows go from winter feeding to spring grazing, is described.2. Two similar groups of cows were fed good quality winter rations at the same high level both before and after calving (about 115% of the Woodman standard after calving), until spring grass was available. One group of 7 cows was then fed for 6 weeks almost entirely on cut grass, returning to winter rations for a further 3 weeks. The control group of 6 cows continued to be fed throughout the experiment on winter rations and both groups remained indoors for the whole of the experiment. The estimated nutrient intake of both groups in all periods was kept substantially constant.3. Changes in milk yield and composition were mainly those associated with advancing stage of lactation, and grass feeding had little specific effect. It is concluded that the increase in milk protein content which is normally observed soon after the start of spring grazing probably results from an improved plane of nutrition and not specifically from an increase in the intake of soluble carbohydrates.4. Increases in the total nitrogen content of the milk as the experiment proceeded were contributed to uniformly by the casein, total albumin and β-lactoglobulin fractions.

scholarly journals Effects of Mindfulness Training on Psychological Capital, Depression, and Procrastination of the Youth Demographic

2020 ◽  
Author(s):  
Li LI ◽  
Mu LI
Keyword(s):  
Psychological Capital ◽  
Depression Scale ◽  
Mindfulness Training ◽  
Control Group ◽  
Five Facet Mindfulness Questionnaire ◽  
Positive Psychological Capital ◽  
Before And After ◽  
Research Objects ◽  
High Level ◽  
Experimental Group

Background: The incidence rate of depression in youth demographic increases year by year. High-level psychological capital is conducive to relieving depression, and mindfulness training is an effective psychotherapy. We aimed to investigate the influences of mindfulness training on psychological capital, depression, and procrastination, thereby providing a direction for psychotherapy in the youth demographic. Method: A questionnaire survey on the youths in four resident communities in the cities of Wuhan and Tai’an in China was carried out in 2020 by sending a self-rating depression scale (SDS). A total of 240 youth with SDS standard scores >53 were filtered out, as the research objects to be assessed by using SDS, positive psychological capital questionnaire (PPQ), five-facet mindfulness questionnaire (FFMQ), and pure procrastination scale (PPS). Results: Two groups showed no statistically significant differences in SDS, PPQ, FFMQ, and PPS scores before intervention. Self-efficacy, toughness, hope, optimism, and PPQ scores of the experimental group were improved significantly after intervention compared with those before the intervention, and they were significantly higher than those of the control group (P<0.05). SDS, PPQ, FFMQ, and PPS scores showed statistically significant differences before and after intervention. SDS and PPS scores of the experimental group after intervention were far lower than those of the control group, while FFMQ score was significantly higher than that of the control group (P<0.05). Conclusion: Mindfulness training can improve psychological capital of the youth demographic effectively and relieve their depression, thereby improving their procrastination.

scholarly journals Training-dependent transfer within a set of nested tasks

2021 ◽  
pp. 174702182199377
Author(s):  
Joseph P Rennie ◽  
Jonathan Jones ◽  
Duncan E Astle
Keyword(s):  
Cognitive Task ◽  
Training Group ◽  
Control Group ◽  
Assessment Task ◽  
Performance Improvements ◽  
Before And After ◽  
Extended Practice ◽  
Feature Overlap ◽  
High Level ◽  
Level Training

Extended practice on a particular cognitive task can boost the performance of other tasks, even though they themselves have not been practised. This transfer of benefits appears to be specific, occurring most when tasks are very similar to those being trained. But what type of similarity is most important for predicting transfer? This question is addressed with a tightly controlled randomised design, with a relatively large sample ( N = 175) and an adaptive control group. We created a hierarchical set of nested assessment tasks. Participants then trained on two of the tasks: one was relatively “low” in the hierarchy requiring just simultaneous judgements of shapes’ spikiness, whereas the other was relatively “high” requiring delayed judgements of shapes’ spikiness or number of spikes in a switching paradigm. Using the full complement of nested tasks before and after training, we could then test whether and how these “low” and “high” training effects cascade through the hierarchy. For both training groups, relative to the control, whether or not an assessment task shared a single specific feature was the best predictor of transfer patterns. For the low-level training group, the overall proportion of feature overlap also significantly predicted transfer, but the same was not true for the high-level training group. Finally, pre-training between-task correlations were not predictive of the pattern of transfer for either group. Together these findings provide an experimental exploration of the specificity of transfer and establish the nature of task overlap that is crucial for the transfer of performance improvements.

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