scholarly journals Discrimination and prediction of polyphenolic compounds and total antioxidant capacity in sorghum grains

2019 ◽  
Vol 27 (1) ◽  
pp. 46-53 ◽  
Author(s):  
Michela Alfieri ◽  
Giovanni Cabassi ◽  
Ephrem Habyarimana ◽  
Fabrizio Quaranta ◽  
Carlotta Balconi ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Condensed Tannins ◽  
Near Infrared ◽  
Total Phenolics ◽  
Rapid Screening ◽  
Antioxidant Compounds ◽  
Total Flavonoids ◽  
Analysis Model ◽  
Total Antioxidant

One hundred and forty-seven sorghum samples, grown in 2015 and 2017, were used to build different near infrared spectroscopic calibration models able to predict total antioxidant capacity, total phenolics, total flavonoids and condensed tannins content. Samples were separated into calibration and validation sets using a nearest neighbours algorithm. The r2pred values ranged from 0.84 (condensed tannins) to 0.95 (total phenolics), whereas the Ratio of Performance to Deviation (RPD) values ranged from 1.9 (total flavonoids) to 3.0 (total phenolics). Comparison of prediction error estimates highlighted the best models with significant differences. Model robustness was tested through a reduction of sample numbers in the calibration set; the highest robustness was found for total antioxidant capacity and total flavonoids. In addition, a partial least squares discriminant analysis model to screen the samples for their tannins level was developed and resulted in good performance; it should be useful to select tannin-free genotypes for the food industry. These models could be used for rapid screening of sorghum breeding genotypes with high antioxidant compounds.

scholarly journals Enhancement of polyphenols and antioxidant and inhibitory properties of Corchorus olitorius and Amaranthus hybridus leaves by the Ultraviolet-C treatment

2020 ◽  
Vol 12 (2) ◽  
pp. 203-210
Author(s):  
Kayode Olayele Karigidi ◽  
Olufunke Omowunmi Adebogun
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Total Phenolics ◽  
Reducing Power ◽  
Total Flavonoids ◽  
Corchorus Olitorius ◽  
Amaranthus Hybridus ◽  
Total Antioxidant ◽  
Ultraviolet C

The effect of the postharvest (Ultraviolet-C) treatment on total phenolics, total flavonoids, total antioxidant capacity, 1,1-diphenyl-2-picrylhydrazyl scavenging activity(DPPH),and reducing power of Corchorus olitorius(CO) and Amaranthus hybridus(AH)was investigated. Their abilities to inhibit induced lipid peroxidation, α-amylase,and α-glucosidase after the ultraviolet-C treatment were also evaluated. The leaves were exposed to ultraviolet-C radiation for 10 min. Total phenolics, total flavonoids, total antioxidant capacity, DPPH scavenging ability,and reducing power were significantly (p < 0.05) increased after the ultraviolet-C treatment. Also,their abilities to inhibit SNP-induced lipid peroxidation, α-amylase and α-glucosidase were also enhanced significantly (p < 0.05). Therefore,apart from using ultraviolet-C radiation in water purification, it can be used to enhance health-promoting components in fruit and vegetables.

Investigating the total antioxidant capacity of gluten-free grains with miniaturized near-infrared spectrometer

NIR news ◽  
2019 ◽  
Vol 30 (5-6) ◽  
pp. 35-38
Author(s):  
Verena Wiedemair ◽  
Christian Wolfgang Huck
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
High Performance ◽  
Near Infrared ◽  
Least Squares Regression ◽  
Test Set ◽  
Total Antioxidant ◽  
Infrared Spectrometer ◽  
Near Infrared Spectrometer ◽  
Infrared Instruments

The use of ever smaller near-infrared instruments is becoming more and more prevalent, since they are cheaper, more versatile and often advertised as high-performance spectrometer. The last claim is rarely verified by independent researchers, which is why the presented work evaluates the performance of three hand-held spectrometers in comparison to a benchtop instrument. Seventy-seven samples comprising buckwheat, millet and oat were investigated for their total antioxidant capacity using Folin–Ciocalteu and near-infrared spectroscopy. Partial least squares regression models were established using cross- and test set validation. Results showed that all instruments were able to predict total antioxidant capacity to some extent. The coefficients of determinations ranged from 0.823 to 0.951 for cross-validated and from 0.849 to 0.952 for test set validated models. Errors for cross-validated models ranged from 1.11 to 2.08 mgGAE/g and for test set validated models from 1.02 to 1.86 mgGAE/g.

scholarly journals Optimization of High-Pressure Extraction Process of Antioxidant Compounds from Feteasca regala Leaves Using Response Surface Methodology

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4209
Author(s):  
Anca Becze ◽  
Vanda Liliana Babalau-Fuss ◽  
Cerasel Varaticeanu ◽  
Cecilia Roman
Keyword(s):  
Response Surface Methodology ◽  
Antioxidant Capacity ◽  
Response Surface ◽  
Total Antioxidant Capacity ◽  
Raw Materials ◽  
Extraction Process ◽  
Extraction Yield ◽  
Antioxidant Compounds ◽  
Operational Conditions ◽  
Total Antioxidant

Circular economy principles are based on the use of by-products from one operation as the raw materials in another. The aim of this work is to obtain extracts with high antioxidant capacity and resveratrol content for the superior capitalization of the biomass of Feteasca regala leaves obtained during vineyard horticultural operations in spring. In order to obtain a high-quality extract at an industrial level, an optimal extraction process is needed. Central composite design (CCD) was used for the experiment design, which contained three independent variables: the ratio of extraction solvent to solid matter, temperature (°C) and time (minutes). The evaluation of extracts was done by measuring the total antioxidant capacity of the extracts using photo-chemiluminescent techniques, and the resveratrol content using liquid chromatography. Process optimization was done using response surface methodology (RSM). Minitab software version 17.0 was used for the design of experiments and data analysis. Regression analysis showed that the model predicts 87.5% of the variation for resveratrol and 96% for total antioxidant capacity (TAC). The temperature had the biggest influence on the extraction yield. The optimal operational conditions for the extraction method applied had the following conditions: ratio e/m 2.92; 43.23 °C and 55.4 min. A maximum value of 34,623 µg ascorbic acid equivalent (AAE) /mL total antioxidant capacity and 182.4 µg/mL resveratrol content were obtained when the optimal extraction parameters where used. The values obtained in experiments proved that by using RSM an accurate model can be obtained for extraction of Feteasca regala leaves.

scholarly journals Proline, Total Antioxidant Capacity, and OsP5CS Gene Activity in Radical and Plumule of Rice are Efficient Drought Tolerance Indicator Traits

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Muhammad Abu Bakar Saddique ◽  
Zulfiqar Ali ◽  
Muhammad Ali Sher ◽  
Babar Farid ◽  
Rao Muhammad Ikram ◽  
...  
Keyword(s):  
Osmotic Stress ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Dry Weight ◽  
Rapid Screening ◽  
Drought Tolerant ◽  
Plant Breeding Program ◽  
Total Antioxidant ◽  
Rice Genotypes ◽  
Crop Germplasm

The success of a plant breeding program is linked with the rapid screening of crop germplasm. In the following study, the germination stage of rice seeds has been examined for the rapid screening of drought-tolerant genotypes. The rice genotypes (10 drought tolerant, 5 moderately drought tolerant, and 5 drought susceptible) were sown in Petri dishes under control and osmotic stress of 15% PEG6000. Data were recorded after four days of sowing for the osmotic stress-induced change in imbibition rate, speed of germination, radical and plumule length, radical and plumule total fresh and dry weight, proline contents, total antioxidant capacity, and malondialdehyde level in radical and plumule of seeds. Moreover, the change in expression of OsP5CS gene was also recorded in one of each drought tolerant, moderately drought tolerant, and drought susceptible genotypes. Under osmotic stress, the level of proline, total antioxidant capacity, and the expression of OsP5CS were increased in drought-tolerant genotypes as compared to moderately drought tolerant and drought susceptible genotypes. While, the change in imbibition rate, speed of germination, radical and plumule length, and fresh and dry weight were not symmetrical in drought tolerant, moderately drought tolerant, and drought susceptible genotypes. In short, the symmetrical change in proline, total antioxidant capacity, and expression of OsP5CS gene within radical and plumule of drought tolerant, moderately drought tolerant, and drought susceptible genotypes can help rapid screening of drought-tolerant rice genotypes.

scholarly journals Chemical Characterization and Antioxidant Activity of Cedrus atlantica Manetti Tar (Atlas Cedar Tar)

2020 ◽  
Vol 8 (2) ◽  
pp. 244-255
Author(s):  
Inssaf Skanderi ◽  
Ourida Chouitah
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Condensed Tannins ◽  
Chemical Characterization ◽  
Antioxidant Power ◽  
Cedrus Atlantica ◽  
Total Antioxidant ◽  
Ferric Reducing Antioxidant Power ◽  
Total Polyphenolic Content

Cedrus atlantica Manetti Tar was analyzed by gas chromatography coupled to mass spectrometry, it was subjected to analyzes to know their total polyphenolic and condensed tannins contents, also their Ferric-reducing antioxidant power and Total antioxidant capacity. Chemical characterization identified 88 constituents where Himachalene and α-atlantone isomers (14.51 % - 4.07 %), Calacorene (3.52 %) and ar-Turmerone 3.35 %, were the major components, the total polyphenolic content and condensed tannins contents were 57.15 ± 0.15 milligrams equivalent of gallic acid /g tar and 4.41 ± 0.05 milligrams equivalent of catechin /g tar respectively .This extract showed remarkable Ferric-reducing antioxidant power with effective concentration equal to 50 ± 0.075 mg /mL ± 0,00028 and total antioxidant capacity equal to 262.75 mg equivalents of ascorbic acid /g tar ± 14,43. The experimental results indicated that our tar has promotive antioxidant activity.

scholarly journals Correlation of Total Polyphenolic Content with Antioxidant Activity of Hydromethanolic Extract and Their Fractions of the Salvia officinalis Leaves from Different Regions of Morocco

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Zakaria Khiya ◽  
Yassine Oualcadi ◽  
Abderrahmane Gamar ◽  
Fatima Berrekhis ◽  
Touria Zair ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Cyclic Voltammetry ◽  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Condensed Tannins ◽  
Salvia Officinalis ◽  
Total Phenolic ◽  
Total Antioxidant ◽  
Pharmaceutical Industries

The purpose of this study is to determine the total content of phenols, flavonoids, and condensed tannins, as well as on the antioxidant activity of the extract, and their fractions were measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), phosphomolybdate reduction (or total antioxidant capacity), and cyclic voltammetry (CV). The hydromethanolic extract of Salvia officinalis showed the highest values of total phenolic (176 mgGAE/g of extract) and condensed tannins (162.53 mgEC/g of extract) from the Boulemane and Khenifra regions, respectively. The results showed that the best DPPH assay was found in the ethyl acetate fraction of Salvia officinalis leaves of the Boulemane region (IC50 = 0.002 mg/ml). For the ethyl acetate and butanolic fractions of Salvia officinalis leaves, those collected from different regions have a better reducing capacity (EC50 = 0.021 mg/ml, respectively). For the total antioxidant capacity, the best activity was found in the aqueous fraction of Salvia officinalis leaves of the Boulemane region (108 mgGAE/g of extract). By the cyclic voltammetry method, hydromethanolic extract of Salvia officinalis leaves from the Boulemane region showed an important result (288.8 mgGAE/g). There was a positive correlation between total phenol content (TPC), condensed tannin content (TCT), and total antioxidant capacity (TAC) (r = 0.932, r = 0.896, respectively). The main compounds that have been identified in the hydromethanolic extract of Salvia officinalis are ascorbic acid, gallic acid, 4-hydroxybenzoic acid, tannic acid, and rutin. Due to their antioxidant property, the leaf extracts from Salvia officinalis are used as natural preservative ingredients in food and/or pharmaceutical industries.

Antioxidant capacity of fractions of alcoholic extracts of sugarcane leaves

2013 ◽  
pp. 165-169
Author(s):  
Juliana Aparecida de Souza-Sartori ◽  
Adna Prado ◽  
Lucimara Fernanda Veiga ◽  
Nathalia Torres ◽  
Severino Matias de Alencar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Silica Gel ◽  
Plant Development ◽  
Final Stage ◽  
Total Antioxidant Capacity ◽  
Solvent Mixture ◽  
Antioxidant Compounds ◽  
Initial Stage ◽  
Total Antioxidant

Extraction of antioxidant compounds present in the top leaves of sugarcane at different stages of plant development showed that the most favorable phase for the extraction is the final stage of maturation of the plant. Flavonoid contents (5.98±0.32mg/mL) and relative antioxidant activity (AArel=257.49±16.94%) similar to the initial stage of maturation were found. Active compounds were chromatographically fractionated over silica gel and elution with solvents of increasing polarity. The antioxidant activity towards -carotene, and the sequestering activity of DPPH (1,1-diphenyl-2-picrylhydrazyl) radicals was determined. The fractions which had the highest total antioxidant capacity (TAC) were obtained from the elution of the solvent mixture: chloroform and ethanol. The results indicate that the top leaves of sugarcane are a possible source of bioactive compounds.

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