scholarly journals Performance and interaction behaviour during visual search on large, high-resolution displays

2013 ◽  
Vol 14 (2) ◽  
pp. 137-147 ◽  
Author(s):  
Roy A Ruddle ◽  
Rhys G Thomas ◽  
Rebecca S Randell ◽  
Phil Quirke ◽  
Darren Treanor
Keyword(s):  
Visual Search ◽  
High Resolution ◽  
Regions Of Interest ◽  
The Other ◽  
Virtual Microscope ◽  
Speed Advantage ◽  
M 12 ◽  
Display Resolution ◽  
Diagnosis Of Diseases ◽  
Physical Navigation

Large, high-resolution displays allow orders of magnitude more data to be visualized at a time than ordinary computer displays. Previous research is inconclusive about the circumstances under which large, high-resolution displays are beneficial and lacks behavioural data to explain inconsistencies in the findings. We conducted an experiment in which participants searched maps for densely or sparsely distributed targets, using 2-million-pixel (0.4 m × 0.3 m), 12-million-pixel (1.3 m × 0.7 m) and 54-million-pixel (3.0 m × 1.3 m) displays. Display resolution did not affect the speed at which dense targets were found, but participants found sparse targets in easily identifiable regions of interest 30% faster with the 54-million-pixel display than with the other displays. This was because of the speed advantage conferred by physical navigation and the fact that the whole dataset fitted onto the 54-million-pixel display. Contrary to expectations, participants found targets at a similar speed and interacted in a similar manner (mostly short panning movements) with the 2- and 12-million-pixel displays even though the latter provided more opportunity for physical navigation, though this may have been because panning used velocity-based control. We are applying these findings to the design of a virtual microscope for the diagnosis of diseases such as cancer.

Computer-Controlled Hrem Alignment Using Automated Diffractogram Analysis

1990 ◽  
Vol 48 (1) ◽  
pp. 532-533
Author(s):  
G.Y. Fan ◽  
O.L. Krivanek
Keyword(s):  
Electron Microscope ◽  
High Resolution ◽  
The Other ◽  
Full Information ◽  
Resolution Electron ◽  
Skilled Operator ◽  
Automatic Alignment ◽  
High Resolution Electron Microscope ◽  
Computer Controlled ◽  
Recorded Image

Full alignment of a high resolution electron microscope (HREM) requires five parameters to be optimized: the illumination angle (beam tilt) x and y, defocus, and astigmatism magnitude and orientation. Because neither voltage nor current centering lead to the correct illumination angle, all the adjustments must be done on the basis of observing contrast changes in a recorded image. The full alignment can be carried out by a computer which is connected to a suitable image pick-up device and is able to control the microscope, sometimes with greater precision and speed than even a skilled operator can achieve. Two approaches to computer-controlled (automatic) alignment have been investigated. The first is based on measuring the dependence of the overall contrast in the image of a thin amorphous specimen on the relevant parameters, the other on measuring the image shift. Here we report on our progress in developing a new method, which makes use of the full information contained in a computed diffractogram.

scholarly journals A workflow for streamlined acquisition and correlation of serial regions of interest in array tomography

BMC Biology ◽  
2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Sergio Gabarre ◽  
Frank Vernaillen ◽  
Pieter Baatsen ◽  
Katlijn Vints ◽  
Christopher Cawthorne ◽  
...  
Keyword(s):  
High Resolution ◽  
Light And Electron Microscopy ◽  
User Interaction ◽  
Regions Of Interest ◽  
Imaging Method ◽  
Array Tomography ◽  
Reference Space ◽  
Cell Processes ◽  
Electron Microscopes ◽  
Specific Tissue

Abstract Background Array tomography (AT) is a high-resolution imaging method to resolve fine details at the organelle level and has the advantage that it can provide 3D volumes to show the tissue context. AT can be carried out in a correlative way, combing light and electron microscopy (LM, EM) techniques. However, the correlation between modalities can be a challenge and delineating specific regions of interest in consecutive sections can be time-consuming. Integrated light and electron microscopes (iLEMs) offer the possibility to provide well-correlated images and may pose an ideal solution for correlative AT. Here, we report a workflow to automate navigation between regions of interest. Results We use a targeted approach that allows imaging specific tissue features, like organelles, cell processes, and nuclei at different scales to enable fast, directly correlated in situ AT using an integrated light and electron microscope (iLEM-AT). Our workflow is based on the detection of section boundaries on an initial transmitted light acquisition that serves as a reference space to compensate for changes in shape between sections, and we apply a stepwise refinement of localizations as the magnification increases from LM to EM. With minimal user interaction, this enables autonomous and speedy acquisition of regions containing cells and cellular organelles of interest correlated across different magnifications for LM and EM modalities, providing a more efficient way to obtain 3D images. We provide a proof of concept of our approach and the developed software tools using both Golgi neuronal impregnation staining and fluorescently labeled protein condensates in cells. Conclusions Our method facilitates tracing and reconstructing cellular structures over multiple sections, is targeted at high resolution ILEMs, and can be integrated into existing devices, both commercial and custom-built systems.

scholarly journals Neutron Imaging Using a Fine-Grained Nuclear Emulsion

2021 ◽  
Vol 7 (1) ◽  
pp. 4
Author(s):  
Katsuya Hirota ◽  
Tomoko Ariga ◽  
Masahiro Hino ◽  
Go Ichikawa ◽  
Shinsuke Kawasaki ◽  
...  
Keyword(s):  
Image Analysis ◽  
High Resolution ◽  
Neutron Detector ◽  
Nuclear Emulsion ◽  
Neutron Imaging ◽  
The Other ◽  
Crystal Oscillator ◽  
Fine Grained ◽  
Cold Neutrons ◽  
Track Analysis

A neutron detector using a fine-grained nuclear emulsion has a sub-micron spatial resolution and thus has potential to be applied as high-resolution neutron imaging. In this paper, we present two approaches to applying the emulsion detectors for neutron imaging. One is using a track analysis to derive the reaction points for high resolution. From an image obtained with a 9 μm pitch Gd grating with cold neutrons, periodic peak with a standard deviation of 1.3 μm was observed. The other is an approach without a track analysis for high-density irradiation. An internal structure of a crystal oscillator chip, with a scale of approximately 30 μm, was able to be observed after an image analysis.

High-Resolution Neutron Spectroscopy for the Investigation of Hydrogen Diffusion and Molecular Rotations in Solids

1993 ◽  
Vol 48 (1-2) ◽  
pp. 406-414
Author(s):  
T. Springer
Keyword(s):  
High Resolution ◽  
Hydrogen Diffusion ◽  
Quantum Effects ◽  
Quantum States ◽  
The Other ◽  
Neutron Spectroscopy ◽  
Impurity Atoms ◽  
Molecular Rotations ◽  
Rotational Motions ◽  
Diffusion Of Hydrogen

Abstract An introductory survey on applications of high-resolution neutron spectroscopy is presented, dealing with the motion of hydrogen in solids, namely concerning (i) random rotational motions or stationary tunneling states of NH+4-ions or CH3-groups, and (ii) diffusion of hydrogen in alloys. For the rotation of hydrogenous groups in solids, at higher temperatures rotational jumps can be found, whereas quantum states are observed by μeV-spectroscopy at temperatures below 50 K. On the other hand, hydrogen diffusion does not reveal pronounced evidence of quantum effects, except for hydrogen in a metal containing impurity atoms.

scholarly journals Structure of the lutein-binding domain of human StARD3 at 1.74 Å resolution and model of a complex with lutein

2016 ◽  
Vol 72 (8) ◽  
pp. 609-618 ◽  
Author(s):  
Martin P. Horvath ◽  
Evan W. George ◽  
Quang T. Tran ◽  
Kody Baumgardner ◽  
Gabe Zharov ◽  
...  
Keyword(s):  
Lipid Transfer Protein ◽  
Transport Proteins ◽  
Binding Domain ◽  
Regions Of Interest ◽  
The Other ◽  
Lipid Transfer ◽  
Transfer Protein ◽  
Biochemical Function ◽  
Rigid Body Docking ◽  
Resolution Structure

A crystal structure of the lutein-binding domain of human StARD3 (StAR-related lipid-transfer protein 3; also known as MLN64) has been refined to 1.74 Å resolution. A previous structure of the same protein determined to 2.2 Å resolution highlighted homology with StARD1 and shared cholesterol-binding character. StARD3 has since been recognized as a carotenoid-binding protein in the primate retina, where its biochemical function of binding lutein with specificity appears to be well suited to recruit this photoprotective molecule. The current and previous structures correspond closely to each other (r.m.s.d. of 0.25 Å), especially in terms of the helix-grip fold constructed around a solvent-filled cavity. Regions of interest were defined with alternate conformations in the current higher-resolution structure, including Arg351 found within the cavity and Ω1, a loop of four residues found just outside the cavity entrance. Models of the complex with lutein generated by rigid-body docking indicate that one of the ionone rings must protrude outside the cavity, and this insight has implications for molecular interactions with transport proteins and enzymes that act on lutein. Interestingly, models with the ∊-ionone ring characteristic of lutein pointing towards the bottom of the cavity were associated with fewer steric clashes, suggesting that steric complementarity and ligand asymmetry may play a role in discriminating lutein from the other ocular carotenoids zeaxanthin andmeso-zeaxanthin, which only have β-ionone rings.

Hands Stencils in El Castillo Cave (Puente Viesgo, Cantabria, Spain). An Interdisciplinary Study

2021 ◽  
pp. 1-21
Author(s):  
Sergio Ripoll ◽  
Vicente Bayarri ◽  
Francisco J. Muñoz ◽  
Ricardo Ortega ◽  
Elena Castillo ◽  
...  
Keyword(s):  
High Resolution ◽  
Real Data ◽  
Digital Processing ◽  
The Other ◽  
Major Step ◽  
The People ◽  
High Resolution Images ◽  
Real Picture ◽  
Interdisciplinary Study ◽  
Very High

Our Palaeolithic ancestors did not make good representations of themselves on the rocky surfaces of caves and barring certain exceptions – such as the case of La Marche (found on small slabs of stone or plaquettes) or the Cueva de Ambrosio – the few known examples can only be referred to as anthropomorphs. As such, only hand stencils give us a real picture of the people who came before us. Hand stencils and imprints provide us with a large amount of information that allows us to approach not only their physical appearance but also to infer less tangible details, such as the preferential use of one hand over the other (i.e., handedness). Both new and/or mature technologies as well as digital processing of images, computers with the ability to process very high resolution images, and a more extensive knowledge of the Palaeolithic figures all help us to analyse thoroughly the hands in El Castillo cave. The interdisciplinary study presented here contributes many novel developments based on real data, representing a major step forward in knowledge about our predecessors.

scholarly journals Improvement in Surface Solar Irradiance Estimation Using HRV/MSG Data

2018 ◽  
Vol 10 (8) ◽  
pp. 1288 ◽  
Author(s):  
Filomena Romano ◽  
Domenico Cimini ◽  
Angela Cersosimo ◽  
Francesco Di Paola ◽  
Donatello Gallucci ◽  
...  
Keyword(s):  
High Resolution ◽  
Solar Irradiance ◽  
Research Council ◽  
National Research Council ◽  
The Other ◽  
Data Channel ◽  
The Difference ◽  
Photovoltaic Plants ◽  
Sky Conditions ◽  
Advanced Model

The Advanced Model for the Estimation of Surface Solar Irradiance (AMESIS) was developed at the Institute of Methodologies for Environmental Analysis of the National Research Council of Italy (IMAA-CNR) to derive surface solar irradiance from SEVIRI radiometer on board the MSG geostationary satellite. The operational version of AMESIS has been running continuously at IMAA-CNR over all of Italy since 2017 in support to the monitoring of photovoltaic plants. The AMESIS operative model provides two different estimations of the surface solar irradiance: one is obtained considering only the low-resolution channels (SSI_VIS), while the other also takes into account the high-resolution HRV channel (SSI_HRV). This paper shows the difference between these two products against simultaneous ground-based observations from a network of 63 pyranometers for different sky conditions (clear, overcast and partially cloudy). Comparable statistical scores have been obtained for both AMESIS products in clear and cloud situation. In terms of bias and correlation coefficient over partially cloudy sky, better performances are found for SSI_HRV (0.34 W/m2 and 0.995, respectively) than SSI_VIS (−33.69 W/m2 and 0.862) at the expense of the greater run-time necessary to process HRV data channel.

scholarly journals An unusual association between Mycobacterium tuberculosis and Aspergillus fumigatus

2016 ◽  
Vol 69 (1) ◽  
Author(s):  
R. Agarwal ◽  
N. Singh ◽  
A.N. Aggarwal
Keyword(s):  
Cystic Fibrosis ◽  
Mycobacterium Tuberculosis ◽  
High Resolution ◽  
Lung Diseases ◽  
Allergic Bronchopulmonary Aspergillosis ◽  
The Other ◽  
Volume Loss ◽  
Computed Tomographic ◽  
Unusual Association ◽  
Computed Tomographic Scan

Allergic bronchopulmonary aspergillosis (ABPA) is rarely described outside the setting of asthma or cystic fibrosis. The occurrence of ABPA in other structural lung diseases included scars of old healed pulmonary tuberculosis (PTB) is also unknown. In this case, we report a 62- year old lady treated for PTB 40 years ago who presented with increasing dyspnea on exertion, cough with expectoration of blackish brown mucus plugs and wheezing. High-resolution computed tomographic scan of the thorax showed parenchymal fibrosis and volume loss in left upper lobe while central bronchiectasis, mosaic attenuation, centrilobular nodules with a tree-in-bud pattern were observed in the other lobes. Investigations revealed a diagnosis of ABPA. The patient was treated with prednisolone and showed a significant response. We review the current literature on this unusual association of previous and cured TB with ABPA, and also discuss the hypothesis of this possible relationship.

scholarly journals Optical Imaging of the Small Intestine Immune Compartments Across Scales

2021 ◽  
Author(s):  
Arielle Planchette ◽  
Cédric Schmidt ◽  
Olivier Burri ◽  
Mercedes Gomez de Agüero ◽  
Aleksandra Radenovic ◽  
...  
Keyword(s):  
Spatial Distribution ◽  
Small Intestine ◽  
High Resolution ◽  
Imaging Modality ◽  
Regions Of Interest ◽  
Label Free ◽  
3 Dimensional ◽  
Wide Range ◽  
Mouse Small Intestine ◽  
Made In

Abstract The limitations of 2D microscopy constrain our ability to observe and understand tissue-wide networks that are, by nature, 3-dimensional. Optical projection tomography enables the acquisition of large volumes (ranging from micrometres to centimetres) in various tissues, with label-free capacities for the observation of auto-fluorescent signals as well fluorescent-labelled targets of interest in multiple channels. We present a multi-modal workflow for the characterization of both structural and quantitative parameters of the mouse small intestine. As proof of principle, we evidence its applicability for imaging the mouse intestinal immune compartment and surrounding mucosal structures. We quantify the volumetric size and spatial distribution of Isolated Lymphoid Follicles (ILFs) and quantify density of villi throughout centimetre long segments of intestine. Furthermore, we exhibit the age- and microbiota-dependence for ILF development, and leverage a technique that we call reverse-OPT for identifying and homing in on regions of interest. Several quantification capabilities are displayed, including villous density in the autofluorescent channel and the size and spatial distribution of the signal of interest at millimetre-scale volumes. The concatenation of 3D image acquisition with the reverse-OPT sample preparation and a 2D high-resolution imaging modality adds value to interpretations made in 3D. This cross-modality referencing technique is found to provide accurate localisation of ROIs and to add value to interpretations made in 3D. Importantly, OPT may be used to identify sparsely-distributed regions of interest in large volumes whilst retaining compatibility with high-resolution microscopy modalities, including confocal microscopy. We believe this pipeline to be approachable for a wide-range of specialties, and to provide a new method for characterisation of the mouse intestinal immune compartment.

scholarly journals Stable individual differences in strategies within, but not between, visual search tasks

2018 ◽  
Author(s):  
Alasdair D F Clarke ◽  
Jessica Irons ◽  
Warren James ◽  
Andrew B. Leber ◽  
Amelia R. Hunt
Keyword(s):  
Individual Differences ◽  
Visual Search ◽  
Search Task ◽  
The Other ◽  
Search Performance ◽  
Search Tasks ◽  
And Performance ◽  
Context Specific ◽  
Test Retest Reliability ◽  
Over Time

A striking range of individual differences has recently been reported in three different visual search tasks. These differences in performance can be attributed to strategy, that is, the efficiency with which participants control their search to complete the task quickly and accurately. Here we ask if an individual's strategy and performance in one search task is correlated with how they perform in the other two. We tested 64 observers in the three tasks mentioned above over two sessions. Even though the test-retest reliability of the tasks is high, an observer's performance and strategy in one task did not reliably predict their behaviour in the other two. These results suggest search strategies are stable over time, but context-specific. To understand visual search we therefore need to account not only for differences between individuals, but also how individuals interact with the search task and context. These context-specific but stable individual differences in strategy can account for a substantial proportion of variability in search performance.

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