Polyethylene Glycol–Based Nanocerium Improves Healing Responses in Excisional and Incisional Wound Models in Rats

2020 ◽  
pp. 153473462091210
Author(s):  
Tara Kardan ◽  
Rahim Mohammadi ◽  
Saeed Taghavifar ◽  
Marzieh Cheraghi ◽  
Ashkan Yahoo ◽  
...  
Keyword(s):  
Wound Healing ◽  
Polyethylene Glycol ◽  
Granulation Tissue ◽  
Wistar Rats ◽  
Wound Area ◽  
Therapeutic Benefits ◽  
Significant Difference ◽  
Excisional Wound ◽  
Wound Healing Model ◽  
Healing Model

Applications of nanotechnology have gained progressive interest for regeneration of injured wound tissue. The aim of the present study was to evaluate effects of polyethylene glycol (PEG)-based nanocerium on excisional and incisional wound models in rats. For excisional wound healing model, 24 male white Wistar rats were randomized into 4 groups of 6 rats each: control group with creation of wounds and no treatment, PEG group with creation of wounds and dressing the wound with PEG, NanoCer group with application of 1 mL nanocerium on the wound, and PEG/NanoCer group with dressing the wound with PEG-based nanocerium. Wound size was measured on days 6, 9, 12, 15, 18, and 21 postsurgery. For incisional wound healing model, 24 healthy male Wistar rats were randomized into 4 groups of 6 rats each the same way in the excisional wound model. Reduction in wound area, hydroxyproline contents, and biomechanical parameters indicated that there was a significant difference ( P > .05) between PEG/NanoCer and other groups. Biomechanical testing was performed on day 9 postsurgery in the incisional model. Biochemical and quantitative histological studies demonstrated that there was a significant difference ( P > .05) between PEG/NanoCer and other groups. PEG/NanoCer offered potential advantages in wound healing acceleration and improvement through angiogenesis stimulation, fibroblast proliferation, and granulation tissue formation on early days of healing phases. Acceleration in wound repair was associated with earlier wound area reduction and enhanced tensile strength of damaged area by rearrangement of granulation tissue and collagen fibers. PEG-based nanocerium could have therapeutic benefits in wound healing.

scholarly journals Morphological Evaluation of Wound Healing Events in the Excisional Wound Healing Model in Rats

BIO-PROTOCOL ◽  
2019 ◽  
Vol 9 (13) ◽  
Author(s):  
Ligia Estevão ◽  
Puebla Cassini-Vieira ◽  
Ana Greice Leite ◽  
Apolônia Bulhões ◽  
Lucíola da Barcelos ◽  
...  
Keyword(s):  
Wound Healing ◽  
Morphological Evaluation ◽  
Excisional Wound ◽  
Wound Healing Model ◽  
Healing Model

scholarly journals Involvement of mast cells, CD68+ and VEGF+ expressions in response to Himatanthus drasticus commercial latex in mice wound healing model

2017 ◽  
Vol 69 (3) ◽  
pp. 513-522
Author(s):  
G.J.L. Santos ◽  
T.C. Ferreira ◽  
A.L.M. Rodrigues ◽  
J.C.C. Freitas ◽  
S.M. Morais ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mast Cells ◽  
Granulation Tissue ◽  
Mineral Oil ◽  
Vegf Expression ◽  
Once Daily ◽  
Vascular Congestion ◽  
Blue Stain ◽  
Wound Healing Model ◽  
Healing Model

ABSTRACT This study aimed to evaluate Himatanthus drasticus latex in a mice wound healing experimental model. Animals were divided into four groups (n=7) according to the treatments: GI - saline 0.9% (control), GII - mineral oil (vehicle), GIII - H. drasticus commercial latex (HdCL) and GIV - H. drasticus mixed isolated fraction (MIF, 1 mg/mL). The treatments were applied topically once daily, 50 µL for 14 consecutive days. Macroscopic lesions were evaluated, considering parameters such as swelling, redness, granulation tissue and reepithelialization. VEGF+, CD68+ expressions and mast cells (Toluidin blue stain) were evaluated. HdCL induced higher contraction and exuberant granulation tissue (P > 0.05). HdCL showed a mild inflammatory process while MIF induced intense infiltrate inflammatory predominantly by lymphocytes, vascular congestion, bleeding and did not presented full reepithelialization. Reorganization of collagen fibers (red picrosirius stain) was observed. CD68+ expression and mast cells were presented as moderate, intense and mild in GI, GIII and GIV, respectively. Neovascularization occurred in all groups, while VEGF+ expression was intense in MIF in relation to HdCL. We concluded that HdCL presents wound healing potential, through modulation of mast cells, CD68+ and VEGF+ expressions that can be associated to triterpenes presence according MIF isolated from HdCL.

Thrombin as important factor for cutaneous wound healing: Comparison of fibrin biomatrices in vitro and in a rat excisional wound healing model

2014 ◽  
Vol 22 (6) ◽  
pp. 740-748 ◽  
Author(s):  
Alfred Gugerell ◽  
Waltraud Pasteiner ◽  
Sylvia Nürnberger ◽  
Johanna Kober ◽  
Alexandra Meinl ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound ◽  
Excisional Wound ◽  
Wound Healing Model ◽  
Healing Model

scholarly journals Avaliação da Exposição e da Interrupção da Nicotina Durante a Cicatrização em Ratos Wistar

2015 ◽  
Vol 8 (1) ◽  
pp. 85
Author(s):  
Ana Rosa Crisci ◽  
Carla Simões Cassemiro ◽  
Cristiane Aparecida Borges ◽  
Fernanda Caravalho Oliveira ◽  
Maria Helena Simões Jorge
Keyword(s):  
Wound Healing ◽  
Light Microscopy ◽  
Granulation Tissue ◽  
Surgical Procedure ◽  
Wistar Rats ◽  
Post Surgery ◽  
Significant Difference ◽  
Post Test ◽  
Collagen Analysis

As evidências clínicas e experimentais demonstram que o uso da nicotina tem efeitos deletéricos sobre a cicatrização. Entretanto, muitas dúvidas ainda existem entre cirurgiões e equipes de saúde em relação ao tempo de interrupção que antecede esses procedimentos. Nesta pesquisa, os ratos Wistar foram divididos em 3 grupos (G1, G2 e G3) que receberam doses diárias de 2 mg/kg de nicotina subcutânea durante 4 semanas. O G1 não interrompeu o uso por dez dias após a cirurgia de retirada de retalho cutâneo; o G2 interrompeu o uso da nicotina uma semana antes da cirurgia; e o G3 interrompeu por duas semanas antes da cirurgia, não fazendo mais uso durante os dez dias após esse procedimento. A análise dos parâmetros de cicatrização na fase proliferativa foi feita através de microscopia de luz e corados pelo H.E. e colorações especiais para análise de colágeno (Tricrômico de Masson e Verhoeff). Encontrou-se no G1 a presença de crosta fibrinoleucocitária, uma visível desorganização do colágeno e um deficiente tecido de granulação quando comparados aos G2 e G3. Dos escores médios dos três grupos obteve-se p=0,028 considerados significativos e no pós-teste Tukey-Kramer foram encontradas diferenças estatísticas significativas entre os G1 e G3 (p<0,05). Na análise do tecido de granulação (p=0,0008) e do colágeno (p=0,049) também se obteve diferença estatística significativa. Pode-se concluir que na interrupção por uma ou duas semanas obteve-se uma melhor e mais eficiente cicatrização, podendo, portanto, extrapolar esses resultados para orientação do pré e pós-cirúrgicos de pacientes tabagistas. Assessment of Exposure and Interruption of Nicotine During Wound Healing in Wistar Rats ABSTRACT: Clinical and experimental evidences show that the use of nicotine has deleterious effects on wound healing, although there are still many doubts among surgeons and health teams with regard to the interruption that should precede interventions. Wistar rats, divided into three groups (G1, G2 and G3), received daily subcutaneous doses of 2 mg/kg nicotine during 4 weeks. G1 did not interrupt use during 10 days after surgery; G2 interrupted nicotine use a week prior to surgery; G3 interrupted during two weeks before surgery and did not take nicotine during ten days after surgical procedure. Wound-healing parameters during the proliferation phase were analyzed by light microscopy and stained with HE and special stains for collagen analysis (Masson´s Trichromium and Verhoeff). A fibrin-leukocyte crust, a visible disorder of collagen and a granulation tissue deficiency occurred in G1 when compared to G2 and G3. Average scores of the three groups (p=0.028) were significant and significant statistical differences between G1 and G3 existed (p<0.05) by Tukey-Kramer´s post-test. Statistically significant difference existed in the granulation (p=0.0008) and collagen (p=0,049) tissues. Results show that a greater and more efficient wound-healing occurred when nicotine was interrupted for one or two weeks, which may be recommended for smoking patients in the pre- and post-surgery periods.

scholarly journals Galvanic microparticles increase migration of human dermal fibroblasts in a wound-healing model via reactive oxygen species pathway

2014 ◽  
Vol 320 (1) ◽  
pp. 79-91 ◽  
Author(s):  
Nina Tandon ◽  
Elisa Cimetta ◽  
Aranzazu Villasante ◽  
Nicolette Kupferstein ◽  
Michael D. Southall ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Wound Healing ◽  
Reactive Oxygen ◽  
Dermal Fibroblasts ◽  
Oxygen Species ◽  
Human Dermal Fibroblasts ◽  
Wound Healing Model ◽  
Healing Model

scholarly journals The assessment and management of hypergranulation

2021 ◽  
Vol 30 (5) ◽  
pp. S6-S10
Author(s):  
Aby Mitchell ◽  
Doris Llumigusin
Keyword(s):  
Wound Healing ◽  
Granulation Tissue ◽  
Wound Surface ◽  
Wound Area ◽  
Nutritional Deficit

Wound healing follows a process of four distinct phases: haemostasis, inflammation, proliferation and maturation. Problems can arise in any of these phases, delaying the wound process. Hypergranulation (also known as overgranulation) during the proliferation phase occurs when granulation tissue over grows beyond the wound surface. Such wounds have a discoloured, raised or swollen appearance and bleed easily. The cause may be infection, the effects of friction on the wound area, nutritional deficit or stress. Treatments will depend on the cause. There is a lack of studies on treatments for hypergranulaton and more research is required.

scholarly journals Transcriptomic Analysis of a Diabetic Skin-Humanized Mouse Model Dissects Molecular Pathways Underlying the Delayed Wound Healing Response

Genes ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 47
Author(s):  
Carlos León ◽  
Francisco García-García ◽  
Sara Llames ◽  
Eva García-Pérez ◽  
Marta Carretero ◽  
...  
Keyword(s):  
Wound Healing ◽  
Functional Enrichment ◽  
Preclinical Model ◽  
Published Data ◽  
Diabetic Patients ◽  
Ulcer Formation ◽  
Histological Features ◽  
Depth Analysis ◽  
Wound Healing Model ◽  
Healing Model

Defective healing leading to cutaneous ulcer formation is one of the most feared complications of diabetes due to its consequences on patients’ quality of life and on the healthcare system. A more in-depth analysis of the underlying molecular pathophysiology is required to develop effective healing-promoting therapies for those patients. Major architectural and functional differences with human epidermis limit extrapolation of results coming from rodents and other small mammal-healing models. Therefore, the search for reliable humanized models has become mandatory. Previously, we developed a diabetes-induced delayed humanized wound healing model that faithfully recapitulated the major histological features of such skin repair-deficient condition. Herein, we present the results of a transcriptomic and functional enrichment analysis followed by a mechanistic analysis performed in such humanized wound healing model. The deregulation of genes implicated in functions such as angiogenesis, apoptosis, and inflammatory signaling processes were evidenced, confirming published data in diabetic patients that in fact might also underlie some of the histological features previously reported in the delayed skin-humanized healing model. Altogether, these molecular findings support the utility of such preclinical model as a valuable tool to gain insight into the molecular basis of the delayed diabetic healing with potential impact in the translational medicine field.

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

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