Parathyroid hormone promotes cartilage healing after free reduction of mandibular condylar fractures by upregulating Sox9

2021 ◽  
pp. 153537022110271
Author(s):  
Yuanyuan Jia ◽  
Liuqin Xie ◽  
Zhenglong Tang ◽  
Dongxiang Wang ◽  
Yun Hu ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Internal Fixation ◽  
Early Stage ◽  
Rabbit Model ◽  
Mandibular Condyle ◽  
Fracture Site ◽  
Control Group ◽  
Condylar Cartilage ◽  
Cartilage Healing ◽  
Experimental Group

After high fractures of the mandibular condyle, the insufficient blood supply to the condyle often leads to poor bone and cartilage repair ability and poor clinical outcome. Parathyroid hormone (PTH) can promote the bone formation and mineralization of mandibular fracture, but its effects on cartilage healing after the free reduction and internal fixation of high fractures of the mandibular condyle are unknown. In this study, a rabbit model of free reduction and internal fixation of high fractures of the mandibular condyle was established, and the effects and mechanisms of PTH on condylar cartilage healing were explored. Forty-eight specific-pathogen-free (SPF) grade rabbits were randomly divided into two groups. In the experimental group, PTH was injected subcutaneously at 20 µg/kg (PTH (1–34)) every other day, and in the control group, PTH was replaced with 1 ml saline. The healing cartilages were assessed at postoperative days 7, 14, 21, and 28. Observation of gross specimens, hematoxylin eosin staining and Safranin O/fast green staining found that every-other-day subcutaneous injection of PTH at 20 µg/kg promoted healing of condylar cartilage and subchondral osteogenesis in the fracture site. Immunohistochemistry and polymerase chain reaction showed that PTH significantly upregulated the chondrogenic genes Sox9 and Col2a1 in the cartilage fracture site within 7–21 postoperative days in the experimental group than those in the control group, while it downregulated the cartilage inflammation gene matrix metalloproteinase-13 and chondrocyte terminal differentiation gene ColX. In summary, exogenous PTH can stimulate the formation of cartilage matrix by triggering Sox9 expression at the early stage of cartilage healing, and it provides a potential therapeutic protocol for high fractures of the mandibular condyle.

scholarly journals Effects of Zoledronic Acid on Physiologic Bone Remodeling of Condylar Part of TMJ: A Radiologic and Histomorphometric Examination in Rabbits

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Ufuk Tatli ◽  
Yakup Üstün ◽  
Mehmet Kürkçü ◽  
Mehmet Emre Benlidayı
Keyword(s):  
Single Dose ◽  
Zoledronic Acid ◽  
Bone Remodeling ◽  
Infusion Pump ◽  
Rabbit Model ◽  
Mandibular Condyle ◽  
Control Group ◽  
New Zealand White Rabbits ◽  
Base Data ◽  
Experimental Group

Objective. The purpose of the present study is to evaluate the effects of systemically administered zoledronic acid (ZA) on the physiological bone remodeling and the microarchitectural parameters of the condylar part of TMJ in a rabbit model.Study Design. Thirty skeletally mature male New Zealand white rabbits were randomly divided into two groups. The experimental group was administered an intravenous, single dose of 0.1 mg/kg ZA diluted with 15 mL of saline in a 15-minute perfusion with an infusion pump. The control group was administered only saline infusion for 15 minutes. All rabbits were sacrificed on the 21st postoperative day. Radiodensitometric and histomorphometric examinations were performed on the harvested mandibular condyles. The data were analyzed statistically.Results. Radiodensitometric findings showed that ZA treatment resulted in a significant increase in the mineralization of mandibular condyle. This result was supported by the histomorphometric findings.Conclusion. The present study has revealed that a temporary delay in the physiological bone remodeling using single dose of ZA increases bone mineral content and makes the microarchitecture of the mandibular condyle more compact. These effects may be regarded as base data and considered in numerous clinical situations including TMJ.

Effect of Mechanical Stress on Rheumatoid Arthritis of the Temporomandibular Joint: a Morphological and Histological Evaluation

2021 ◽  
Author(s):  
Kohei Nagai ◽  
Takenobu Ishii ◽  
Yasushi Nishii
Keyword(s):  
Rheumatoid Arthritis ◽  
T Cell ◽  
Mechanical Stress ◽  
Temporomandibular Joint ◽  
Mandibular Condyle ◽  
Γδ T Cells ◽  
Histological Evaluation ◽  
Control Group ◽  
Condylar Cartilage ◽  
Layer Width

Abstract Background Rheumatoid arthritis of the temporomandibular joint (TMJ-RA) has been reported to have a larger incidence range than systemic rheumatoid arthritis (RA). The presence or absence of mechanical stress (MS) is considered a factor in this. In this study, we hypothesized that TMJ-RA develops or worsens when excessive MS is applied to the temporomandibular joint of RA mouse models. We aimed to clarify the relationship between TMJ-RA and MS through morphological and histological evaluation. Methods Collagen antibody-induced arthritis (CAIA) was induced in male DBA/1JNCrlj 9–12 weeks old mice by administering Type II collagen antibody and lipopolysaccharide to produce RA model mice. MS was applied to the mandibular condyle. The group was separated into non-RA (control group (N = 5) and MS group (N = 5)), and RA group (CAIA group (N = 5)and CAIA MS group (N = 5)). To confirm the morphological changes in the mandibular condyle, micro-CT imaging was performed. Histological evaluation of the TMJ was performed by hematoxylin and eosin staining for condylar cartilage cell layer thickness, Safranin O staining for proteoglycans, and tartrate-resistant acidic phosphatase staining for osteoclast count. Immunohistochemical evaluation was performed to assess the localization of cartilage destruction enzymes using ADAMTS-5 (a disintegrin and metalloproteinase with thrombospondin motifs) antibody. Additionally, CD3 (cluster of differentiation), CD45, and γδ TCR (T cell receptor) antibodies were used to localize and identify the type of lymphocytes. Results In the CAIA MS model, a three-dimensional analysis of the temporomandibular joint by microcomputer tomography showed a crude change in the surface of the mandibular condyle. Histological examination revealed a decrease in the chondrocyte layer width and an increase in the number of osteoclasts in the mandibular condyle. T cell accumulation was observed, and γδ T cell involvement was confirmed. Conclusions In the CAIA model, the TMJ was less sensitive to the initiation of RA. However, the results suggested that it was exacerbated by MS, and that γδ T cells may be involved in TMJ-RA.

Atelocollagen Enhances the Healing of Rotator Cuff Tendon in Rabbit Model

2017 ◽  
Vol 45 (9) ◽  
pp. 2019-2027 ◽  
Author(s):  
Dong-Sam Suh ◽  
Jun-Keun Lee ◽  
Ji-Chul Yoo ◽  
Sang-Hun Woo ◽  
Ga-Ram Kim ◽  
...  
Keyword(s):  
Rotator Cuff ◽  
Rapid Development ◽  
Rabbit Model ◽  
Supraspinatus Tendon ◽  
Histological Evaluation ◽  
Control Group ◽  
Patch Type ◽  
Load To Failure ◽  
Cuff Repair ◽  
Experimental Group

Background: Failure of rotator cuff healing is a common complication despite the rapid development of surgical repair techniques for the torn rotator cuff. Purpose: To verify the effect of atelocollagen on tendon-to-bone healing in the rabbit supraspinatus tendon compared with conventional cuff repair. Study Design: Controlled laboratory study. Methods: A tear of the supraspinatus tendon was created and repaired in 46 New Zealand White rabbits. They were then randomly allocated into 2 groups (23 rabbits per group; 15 for histological and 8 for biomechanical test). In the experimental group, patch-type atelocollagen was implanted between bone and tendon during repair; in the control group, the torn tendon was repaired without atelocollagen. Each opposite shoulder served as a sham (tendon was exposed only). Histological evaluation was performed at 4, 8, and 12 weeks. Biomechanical tensile strength was tested 12 weeks after surgery. Results: Histological evaluation scores of the experimental group (4.0 ± 1.0) were significantly superior to those of the control group (7.7 ± 2.7) at 12 weeks ( P = .005). The load to failure was significantly higher in the experimental group (51.4 ± 3.9 N) than in the control group (36.4 ± 5.9 N) ( P = .001). Conclusion: Histological and biomechanical studies demonstrated better results in the experimental group using atelocollagen in a rabbit model of the supraspinatus tendon tear. Clinical Relevance: Atelocollagen patch could be used in the cuff repair site to enhance healing.

scholarly journals Pengaruh Pendidikan Kesehatan Tentang Inspeksi Asam Asetat (IVA) Terhadap Motivasi Wus dalam Deteksi Kanker Serviks di Puskesmas Putri Ayu Jambi

2019 ◽  
Vol 19 (1) ◽  
pp. 42
Author(s):  
Hesty Hesty ◽  
Rahmah Rahmah ◽  
Nurfitriani Nurfitriani
Keyword(s):  
Cervical Cancer ◽  
Health Education ◽  
Early Stage ◽  
Sampling Technique ◽  
World Health ◽  
Control Group ◽  
P Value ◽  
Childbearing Age ◽  
Control Group Design ◽  
Experimental Group

World Health Organization (WHO), cervical cancer is the number two cause of death in the world for women from all existing cancer. Every two minutes a woman dies of this disease. This cancer attack at an early stage does not cause any symptoms. That is why, why does this type of cancer fall into the category of the silent killer. One of the promotive and preventive efforts in women of childbearing age as primary prevention can be through the provision of health education about cervical cancer using the Acetic Acid Inspection (IVA) method. The purpose of this study is to find out whether there is an influence of health education about IVA on WUS motivation in cervical cancer detection in health centers. Putri Ayu Jambi. This type of quantitative research with non randomized pretest-posttest with control group design, with purposive sampling technique totaling 22 WUS, 11 people as controls and 11 experimental people. The instrument in the form of questionnaires was analyzed by univariate and bivariate Paired Test t-test, which was conducted from April to July 2018.  The results of the study revealed that of the 22 WUSs studied, the average motivation of the pretest in the experimental group was 41.1818 and the control group was 39.9091, while the results of the posttest of the experimental group after being given counseling and leaflets increased by 42.9091 with p-value 0.034. There is an influence of health education about IVA tests on WUS motivation in detecting cervical cancer. It is suggested that the Puskesmas can improve activities in planning disease control programs, especially carrying out counseling and counseling both in the puskesmas building and in the working area of the puskesmas by involving cadres in their environment so that WUS is moved to take the IVA examination.

14 ENHANCEMENT OF THE EARLY RECOVERY OF OVARY AND UTERUS FUNCTIONS BY TREATMENT OF eCG AND POVIDONE-IODINE IN POSTPARTUM JAPANESE BLACK CATTLE

2017 ◽  
Vol 29 (1) ◽  
pp. 114
Author(s):  
H. Matsuda ◽  
T. Yamanouchi ◽  
Y. Goto ◽  
M. Ohtake ◽  
Y. Hashiyada
Keyword(s):  
Povidone Iodine ◽  
Early Stage ◽  
Beef Cows ◽  
Innovative Technology ◽  
Control Group ◽  
Japanese Black Cattle ◽  
Early Recovery ◽  
Exact Test ◽  
Animal Reproduction ◽  
Experimental Group

The aim of this study was to evaluate the effect of administration of eCG and intrauterine infusion of povidone-iodine to promote the early recovery of ovary and uterus functions in early stage after delivery on postpartum beef cows. Thirty-three postpartum Japanese Black cows without retention of placenta were used for this investigation. After delivery, cows nursed colostrums to calves for several hours in a pen, and then were separated from calves and returned into a barn managed in a group. In experimental group (n = 14), on Day 10 (Day 0 = day of parturition), an intramuscular administration of 500 IU of eCG and infusion of 30 mL of 2% povidone-iodine into the uterus body were conducted. The untreated cows delivered in the same period were compared as a control group (n = 19). Cows were inseminated artificially using commercial Japanese Black frozen–thawed semen when standing oestrus was detected until Day 90. Pregnancy diagnosis was performed 30 days after insemination by ultrasonography. For all of the experimental group and 8 cows in the control group, the diameter of follicles >8 mm and the diameter of cross-section between endometrium in uterus of the implanted side at the point of ~2 cm from bifurcation of the uterus were measured using an ultrasound scanning machine connected to a 7.5-MHz convex probe. This monitoring was carried out every 10 days from Day 10 until the day of first oestrus (= insemination) or until Day 80 for non-returned oestrus cows. Data were analysed using Fisher’s exact test and ANOVA. The ratio of returned oestrus by Day 90 was 100% (14/14) in experimental group and 89.5% (17/19) in control group. The first oestrus day postpartum in the experimental group and the control group was the almost same: 52.9 ± 12.1 (24–88) days and 57.2 ± 19.9 (29–78) days, respectively. The conception rate until Day 90 tended to be higher (P = 0.07) in the experimental group (78.6%, 11/14) than in the control group (47.4%, 9/19). The days inseminated to cows that were conceived, was almost same between the experimental group (57.5 ± 18.6, 24–87 d) and the control group (55.4 ± 12.9, 36–78 d). The ratio of cows observed follicles more than 8 mm, was higher in the experimental group than control group on Day 50 (90.9% v. 50.0%; P = 0.09) and Day 60 (66.7% v. 0%; P < 0.05), respectively. The diameter of uterus tended to be lesser in the experimental group than in the control group on Day 30 (16.9 ± 2.8 mm v. 19.2 ± 2.8 mm; P = 0.09), and that regressed linearly to Day 30 in the experimental group however that was prolonged until Day 40 (16.4 ± 1.7 mm) in the control group. These results indicate that administration of eCG and uterus infusion of povidone-iodine at an early stage postpartum may promote the early recovery of ovary and uterus functions in beef cows. This research was supported by a grant from the Research Program of MAFF, (REP1004) Development of Innovative Technology for Animal Reproduction.

scholarly journals Morphometric evaluation of condylar cartilage of growing rats in response to mandibular retractive forces

2013 ◽  
Vol 18 (4) ◽  
pp. 113-119 ◽  
Author(s):  
Milena Peixoto Nogueira de Sá ◽  
Jacqueline Nelisis Zanoni ◽  
Carlos Luiz Fernandes de Salles ◽  
Fabrício Dias de Souza ◽  
Uhana Seifert Guimarães Suga ◽  
...  
Keyword(s):  
Functional Class ◽  
Control Group ◽  
Class Iii ◽  
Condylar Cartilage ◽  
Time Intervals ◽  
Undifferentiated Cells ◽  
Male Wistar Rats ◽  
Mandibular Retrusion ◽  
Experimental Group ◽  
Morphometric Evaluation

INTRODUCTION: The mandibular condylar surface is made up of four layers, i.e., an external layer composed of dense connective tissue, followed by a layer of undifferentiated cells, hyaline cartilage and bone. Few studies have demonstrated the behavior of the condylar cartilage when the mandible is positioned posteriorly, as in treatments for correcting functional Class III malocclusion. OBJECTIVE: The aim of this study was to assess the morphologic and histological aspects of rat condyles in response to posterior positioning of the mandible. METHODS: Thirty five-week-old male Wistar rats were selected and randomly divided into two groups: A control group (C) and an experimental group (E) which received devices for inducing mandibular retrusion. The animals were euthanized at time intervals of 7, 21 and 30 days after the experiment had began. For histological analysis, total condylar thickness was measured, including the proliferative, hyaline and hypertrophic layers, as well as each layer separately, totaling 30 measurements for each parameter of each animal. RESULTS: The greatest difference in cartilage thickness was observed in 21 days, although different levels were observed in the other periods. Group E showed an increase of 39.46% in the total layer, reflected by increases in the thickness of the hypertrophic (42.24%), hyaline (46.92%) and proliferative (17.70%) layers. CONCLUSIONS: Posteriorly repositioning the mandible produced a series of histological and morphological responses in the condyle, suggesting condylar and mandibular adaptation in rats.

scholarly journals Effects ofMATAS HopscotchTechnique in the Teaching of Fractions and Error Patterns Made by Year 5 Pupils

2014 ◽  
Vol 2014 ◽  
pp. 1-8
Author(s):  
Devaki Periasamy ◽  
Kamariah Abu Bakar ◽  
Ahmad Fauzi Mohd Ayub
Keyword(s):  
Early Stage ◽  
Control Group ◽  
Error Patterns ◽  
Post Test ◽  
Quasi Experimental ◽  
Experimental Group ◽  
Types Of Error

MATAS Hopscotchtechnique was created to solve the subtraction of three types of fractions. The study involved 56 pupils from two Year 5 classes. A quasi-experimental, nonrandomized control group, pretest-posttest delayed post-test was conducted on two intact groups, randomly assigned into control and experimental groups. A pretest was administered at the early stage of this study. The study described types of error made by the pupils in solving the subtraction of fractions. Rubrics, Hodes and Notling (1998), were used to describe types of error made by the pupils in the pretest and posttest. The findings in the pretest showed both groups made concept, directions, and careless errors. However, in the posttest, the experimental group made careless errors while the control group made concept, directions, and careless errors. The number of errors made by the control group was higher than that made by the experimental group.

scholarly journals Esubacute acidosis in rumen of high-yield dairy cows: Prevalence and prevention

2008 ◽  
Vol 62 (1-2) ◽  
pp. 43-51
Author(s):  
Branko Petrujkic ◽  
Horea Samanc ◽  
Milan Adamovic ◽  
Danijela Kirovski ◽  
Ivan Vujanac ◽  
...  
Keyword(s):  
Ph Value ◽  
Early Stage ◽  
Rumen Content ◽  
High Yield ◽  
Control Group ◽  
Buffer Effect ◽  
Stage Of Lactation ◽  
Rumen Ph ◽  
Rumen Acidosis ◽  
Experimental Group

The objective of the investigations presented in this paper was to establish the frequency of the incidence of subacute acidosis in the rumen of cows (SARA) in the first three months of lactation and the possibilities for its prevention using a mineral mix based on bentonite, zeolite, magnesium oxide, and sodium bicarbonate (Mix plus). The values obtained for the rumen pH content show that subacute rumen acidosis occurs in in 20 percent of the examined cows in the early stage of lactation. For these investigations, cows in early stages of lactation were chosen and divided into 2 groups. Cows of the experimental group were administered a fodder mix which contained the mineral mix for a buffer effect (Mix plus). The average values of the rumen pH content in the control and the experimental group of cows at the beginning and on the 30th day of the experiment were approximately the same and did not differ significantly (p>0.05). On the 60th day of the experiment, the values for the electrochemical reaction of the rumen content for the control group amounted to an average of 6.219?0.18, and for the experimental group of cows it was 6.772?0.23. The obtained difference was statistically very significant (p<0.001). At the end of the experiment, on the 90th day, the average pH value of the rumen content of cows of the control group was 6.308?0.16, while this value in the experimental group of cows was significantly higher and amounted to 6.676?0.29 (p<0.01).

scholarly journals The Effect of Subconjunctival Bevacizumab on Angiogenesis in Rabbit Model

2020 ◽  
Vol 55 (4) ◽  
pp. 290
Author(s):  
Nurwasis Nurwasis ◽  
Diana Yuliawati ◽  
Evelyn Komaratih ◽  
Heriyawati Heriyawati
Keyword(s):  
Blood Vessels ◽  
Healing Process ◽  
Rabbit Model ◽  
Zealand White Rabbit ◽  
Control Group ◽  
Histopathological Analysis ◽  
Subconjunctival Injection ◽  
Significant Difference ◽  
The Mean ◽  
Experimental Group

The wound healing process is major cause of glaucoma surgery failure and enhances the incapability of controlling the IOP. This aim of study was to evaluate the angiogenesis effect of Bevacizumab injection on the amount and density of blood vessels in the rabbit model after trabeculectomy. It was a true experimental study using 16 eyes of 16 New Zealand White Rabbit (Oryctolagus cuniculus) that divided into two groups. The control group was treated with 0.05 ml of Balanced Saline Solution (BSS). The experimental group was treated with subconjunctival injection of 1.25 mg Bevacizumab in 0.05 BSS. All rabbit was sacrificed, and the eye was enucleated. Thus, the bleb area was dissected after 14 days. Histopathological analysis was performed to evaluate the amount and density of blood vessels. The mean amount of blood vessels in the control group was 22.63 ± 11.02, and the experimental group was 14,75 ± 4.92 (p=0.043). The mean of blood vessel density in the control group was 19.10 ± 1.69 %, and the experimental group was 16.53 ± 2.90 % (p=0.029)%. There was a statistically significant difference between the two groups (p<0.05). Subconjunctival bevacizumab in the rabbit model reduces the amount and density of blood vessels compared with subconjunctival BSS injection.

scholarly journals To Assess the Effectiveness of Breathing Exercise on Labour Pain and Behavioral Response of Primi Gravida Mothers during First Stage of Labour, at Selected Maternity Hospitals

2021 ◽  
pp. 321-326
Author(s):  
Shalini Moon ◽  
Manjusha Mahakarkar ◽  
Pradnya Sakle
Keyword(s):  
Behavioral Response ◽  
Early Stage ◽  
Labour Pain ◽  
Demographic Variables ◽  
Control Group ◽  
Final Conclusion ◽  
Intensity Level ◽  
Breathing Exercise ◽  
Maternity Hospitals ◽  
Experimental Group

Background of the Study: Labour is tough and headaches occur, however mother's our bodies are designed to supply birth. The shape of the pelvis, hormones, effective muscle mass and further all paintings collectively that may help mother to convey her child into the globe - before, within the course of and after childbirth. Objectives: 1. To assess pain intensity level among the Primi gravida mothers during first stage of labour in experimental and control group.2. To assess the behavioral response of primi gravid mothers during 1st stage of labour in control and Experimental group. 3. To determine the effectiveness of breathing exercise on labour pain among primi gravida mothers during first stage of labour.4. To determine the effectiveness of breathing exercise on behavioral response among primi gravida mothers during first stage of labour. 5. To determine the association between selected demographic variables and pain of primi gravida mothers during first stage of labour.6. To determine the association between selected demographic variables and behavioral response of prim gravid mothers during first stage of labour. Material and Methods: The design of this research study was based on a True Experimental research design with a pre-test and post-test control group. A total of 400 mothers were included in the study (200 in experimental group and 200 in control group). The samples were chosen using the probability simple random sampling technique. The data will be gathered using Baseline Proforma and an observational check list. Expected Results: At selected Maternity hospitals, this study will assess the effects of respiratory exercise on Childbirth pain and behavioral responses of primi gravida moms during the early stage of childbirth. After intervention, the degree of discomfort in primi gravida women should be reduced, and their behavioral reaction should improve. Conclusion:  Final conclusion is drawn from the ultimate results of the statistical review.

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