scholarly journals Circulating microRNAs in serum as novel biomarkers for osteoporosis: a case-control study

2020 ◽  
Vol 12 ◽  
pp. 1759720X2095333
Author(s):  
Yi Shuai ◽  
Li Liao ◽  
Xiaoxia Su ◽  
Nanxi Sha ◽  
Xiaobo Li ◽  
...  
Keyword(s):  
Type I Collagen ◽  
Case Control Study ◽  
Disease Diagnosis ◽  
Case Control ◽  
Type I ◽  
Circulating Mirnas ◽  
Circulating Micrornas ◽  
Candidate Mirnas ◽  
Novel Biomarkers ◽  
Control Study

Aims: Osteoporosis is underdiagnosed because of the lack of a convenient diagnostic method. Circulating microRNAs (miRNAs) emerge as novel biomarkers for disease diagnosis. Here, we conducted a case-control study that included a total of 448 serum samples collected from 182 healthy participants, 132 osteopenia participants, and 134 osteoporosis patients. Methods: Circulating miRNAs dysregulated during osteoporosis were screened and analyzed in three randomly determined sub-cohorts: the discovery cohort identified 22 candidate miRNAs; the training cohort tested the candidate miRNAs and constructed Index 1, comprising five miRNAs by logistic regression, and Index 2, comprising four miRNAs, was developed by linear combination. Results: Both indices were tested in the validation cohort and showed statistically significant results in distinguishing osteoporosis patients from healthy and osteopenic patients. Moreover, Index 1 also showed improved performance over traditional bone turnover biomarkers type I pro-collagen (tPINP) and type I collagen (β-CTx). Conclusion: In conclusion, circulating miRNAs are potential biomarkers for osteoporosis. The diagnostic panel of circulating miRNAs could be a complementary method for dual-energy X-ray absorptiometry (DXA) in mass screening and routine examination to enhance the osteoporosis detection rate.

scholarly journals Some patients with intracranial aneurysms have a reduced type III/type I collagen ratio: A case-control study

Neurology ◽  
1997 ◽  
Vol 49 (6) ◽  
pp. 1546-1551 ◽  
Author(s):  
J.S.P. van den Berg ◽  
M. Limburg ◽  
G. Pals ◽  
F. Arwert ◽  
A. Westerveld ◽  
...  
Keyword(s):  
Intracranial Aneurysms ◽  
Type I Collagen ◽  
Case Control Study ◽  
Case Control ◽  
Type I ◽  
Type Iii ◽  
Control Study

Circulating miRNAs in diabetic kidney disease: case–control study and in silico analyses

2018 ◽  
Vol 56 (1) ◽  
pp. 55-65 ◽  
Author(s):  
Taís S. Assmann ◽  
Mariana Recamonde-Mendoza ◽  
Aline R. Costa ◽  
Márcia Puñales ◽  
Balduíno Tschiedel ◽  
...  
Keyword(s):  
Kidney Disease ◽  
In Silico ◽  
Diabetic Kidney Disease ◽  
Case Control Study ◽  
Case Control ◽  
Circulating Mirnas ◽  
Diabetic Kidney ◽  
Disease Case ◽  
In Silico Analyses ◽  
Control Study

scholarly journals Peri-implantitis and extracellular matrix antibodies: A case–control study

2017 ◽  
Vol 11 (03) ◽  
pp. 340-344 ◽  
Author(s):  
Piero Papi ◽  
Stefano Di Carlo ◽  
Daniele Rosella ◽  
Francesca De Angelis ◽  
Mario Capogreco ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Case Control Study ◽  
Test Group ◽  
Case Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Fisher Exact Test ◽  
Type I ◽  
Significant Difference ◽  
Control Study

ABSTRACT Objective: The aim of this case–control study was to compare patients with a healthy peri-implant environment and patients affected by peri-implantitis, evaluating the occurrence of antibodies to extracellular matrix (ECM) molecules. The authors hypothesized the presence of ECM autoantibodies in serum of peri-implantitis patients. Materials and Methods: Patients were divided into two groups: one with dental implants with a diagnosis of peri-implantitis and one control group with implants classified as being “healthy.” Enzyme-linked immunosorbent assay was performed on patients' sera to detect human antibodies to type I, III, IV, and V collagens, laminin, and fibronectin. Fisher exact test was performed to evaluate statistical association, with a significant P < 0.05. Results: Forty-two patients were enrolled in this study, 27 females (64.28%) and 15 males (35.72%) with a mean age of 53 ± 29.69 years (age range 32–74). The presence of antibodies to CIII was recorded in 6/21 (28.57%) patients of test group, compared to just 2/21 (9.52%) for the control group, showing a statistically significant difference (P < 0.05). Other antibodies tested were found to be not statistically significant or absent. Conclusions: Within the limitations of this study, it can be concluded that further studies, with larger sample and different design, are necessary to address the research purpose, evaluating possible associations between anti-ECM antibodies and peri-implantitis.

scholarly journals Circulating microRNAs in Sera Correlate with Soluble Biomarkers of Immune Activation but Do Not Predict Mortality in ART Treated Individuals with HIV-1 Infection: A Case Control Study

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0139981 ◽  
Author(s):  
Daniel D. Murray ◽  
Kazuo Suzuki ◽  
Matthew Law ◽  
Jonel Trebicka ◽  
Jacquie Neuhaus ◽  
...  
Keyword(s):  
Immune Activation ◽  
Case Control Study ◽  
Case Control ◽  
Circulating Micrornas ◽  
Hiv 1 ◽  
Control Study

Infections and risk of type I diabetes in childhood: A population-based case–control study

2002 ◽  
Vol 18 (5) ◽  
pp. 425-430 ◽  
Author(s):  
Emma Altobelli ◽  
Reimondo Petrocelli ◽  
Alberto Verrotti ◽  
Marco Valenti
Keyword(s):  
Case Control Study ◽  
Type I Diabetes ◽  
Population Based ◽  
Case Control ◽  
Type I ◽  
Diabetes In Childhood ◽  
Control Study

scholarly journals Association of Toll-like receptor 7 variants with life-threatening COVID-19 disease in males: findings from a nested case-control study

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Chiara Fallerini ◽  
Sergio Daga ◽  
Stefania Mantovani ◽  
Elisa Benetti ◽  
Nicola Picchiotti ◽  
...  
Keyword(s):  
Gene Expression ◽  
Case Control Study ◽  
Susceptibility Gene ◽  
Case Control ◽  
Type I ◽  
Loss Of Function ◽  
Extreme Phenotype ◽  
Male Patients ◽  
Nested Case Control ◽  
Control Study

Background: Recently, loss-of-function variants in TLR7 were identified in two families in which COVID-19 segregates like an X-linked recessive disorder environmentally conditioned by SARS-CoV-2. We investigated whether the two families represent the tip of the iceberg of a subset of COVID-19 male patients. Methods: This is a nested case-control study in which we compared male participants with extreme phenotype selected from the Italian GEN-COVID cohort of SARS-CoV-2-infected participants (<60y, 79 severe cases versus 77 control cases). We applied the LASSO Logistic Regression analysis, considering only rare variants on young male subsets with extreme phenotype, picking up TLR7 as the most important susceptibility gene. Results: Overall, we found TLR7 deleterious variants in 2.1% of severely affected males and in none of the asymptomatic participants. The functional gene expression profile analysis demonstrated a reduction in TLR7-related gene expression in patients compared with controls demonstrating an impairment in type I and II IFN responses. Conclusion: Young males with TLR7 loss-of-function variants and severe COVID-19 represent a subset of male patients contributing to disease susceptibility in up to 2% of severe COVID-19.

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