scholarly journals Structural Characterization of Dietary Fiber of Green Chalcumra (Benincasa Hispida) Fruit by NMR Spectroscopic Analysis

2009 ◽  
Vol 4 (4) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Debsankar Das ◽  
Subhas Mondal ◽  
Debabrata Maiti ◽  
Sadhan K. Roy ◽  
Syed S. Islam
Keyword(s):  
Dietary Fiber ◽  
Spectroscopic Analysis ◽  
Periodate Oxidation ◽  
Spectroscopic Studies ◽  
Water Soluble ◽  
Molar Ratio ◽  
Methylation Analysis ◽  
Soluble Dietary Fiber ◽  
Benincasa Hispida

A water-soluble dietary fiber was isolated from the hot aqueous extract of Chalcumra ( Benincasa hispida) fruit. The polysaccharide was found to contain D-galactose and D-methyl galacturonate in a molar ratio of 2:1. On the basis of acid hydrolysis, methylation analysis, periodate oxidation, and NMR spectroscopic studies (1H, 13C, TOCSY, DQF-COSY, NOESY, ROESY, HMQC, and HMBC), the repeating unit of the polysaccharide was established as →4)-β-D-Gal p-(1→4)-β-D-Gal p-(1→2)-α-D-Gal pA6Me-(1→.

scholarly journals Characterization of Calcium Compounds inOpuntia ficus indicaas a Source of Calcium for Human Diet

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Isela Rojas-Molina ◽  
Elsa Gutiérrez-Cortez ◽  
Moustapha Bah ◽  
Alejandra Rojas-Molina ◽  
César Ibarra-Alvarado ◽  
...  
Keyword(s):  
Dietary Fiber ◽  
Energy Dispersive Spectrometry ◽  
Molar Ratio ◽  
Total Calcium ◽  
X Ray Diffraction ◽  
Soluble Dietary Fiber ◽  
Minimum Concentration ◽  
Insoluble Dietary Fiber ◽  
Calcium Compounds

Analyses of calcium compounds in cladodes, soluble dietary fiber (SDF), and insoluble dietary fiber (IDF) ofOpuntia ficus indicaare reported. The characterization of calcium compounds was performed by using Scanning Electron Microscopy, Energy Dispersive Spectrometry, X-ray diffraction, and infrared spectroscopy. Atomic Absorption Spectroscopy and titrimetric methods were used for quantification of total calcium and calcium compounds. Whewellite (CaC2O4·H2O), weddellite (CaC2O4·(H2O)2.375), and calcite (CaCO3) were identified in all samples. Significant differences (P≤0.05) in the total calcium contents were detected between samples. CaC2O4·H2O content in cladodes and IDF was significantly higher (P≤0.05) in comparison to that observed in SDF, whereas minimum concentration of CaCO3was detected in IDF with regard to CaCO3contents observed in cladodes and SDF. Additionally, molar ratio oxalate : Ca2+in all samples changed in a range from 0.03 to 0.23. These results support that calcium bioavailability inO. ficus indicamodifies according to calcium compounds distribution.

Characterization of Chemical Impurities in Glutaraldehyde

1975 ◽  
Vol 33 ◽  
pp. 620-621
Author(s):  
B. J. Grenon ◽  
A. J. Tousimis
Keyword(s):  
Glutaric Acid ◽  
Spectroscopic Analysis ◽  
Aldol Condensation ◽  
Condensation Product ◽  
Amorphous Solid ◽  
Water Soluble ◽  
Impurity Component ◽  
Chemical Impurities ◽  
Soluble Liquid

Ever since the introduction of glutaraldehyde as a fixative in electron microscopy of biological specimens, the identification of impurities and consequently their effects on biologic ultrastructure have been under investigation. Several reports postulate that the impurities of glutaraldehyde, used as a fixative, are glutaric acid, glutaraldehyde polymer, acrolein and glutaraldoxime.Analysis of commercially available biological or technical grade glutaraldehyde revealed two major impurity components, none of which has been reported. The first compound is a colorless, water-soluble liquid with a boiling point of 42°C at 16 mm. Utilizing Nuclear Magnetic Resonance (NMR) spectroscopic analysis, this compound has been identified to be — dihydro-2-ethoxy 2H-pyran. This impurity component of the glutaraldehyde biological or technical grades has an UV absorption peak at 235nm. The second compound is a white amorphous solid which is insoluble in water and has a melting point of 80-82°C. Initial chemical analysis indicates that this compound is an aldol condensation product(s) of glutaraldehyde.

Glucan isolated from leaves of Althaea officinalis L.

1983 ◽  
Vol 48 (7) ◽  
pp. 2082-2087 ◽  
Author(s):  
Alžbeta Kardošová ◽  
Jozef Rosík ◽  
Rudolf Toman ◽  
Peter Capek
Keyword(s):  
Medicinal Plant ◽  
Periodate Oxidation ◽  
Linear Structure ◽  
Water Soluble ◽  
Partial Hydrolysis ◽  
Methylation Analysis ◽  
Glycosidic Bonds ◽  
Nmr Data ◽  
Althaea Officinalis ◽  
C Nmr

A water-soluble low-molecular D-glucan was isolated from leaves of the medicinal plant marsh-mallow (Althaea officinalis L.). The results of methylation analysis, partial hydrolysis, periodate oxidation, and 13C NMR data indicated a virtually linear structure with α-(1→6) glycosidic bonds.

scholarly journals Characterization of two cell-wall polysaccharides from Fusicoccum amygdali

1971 ◽  
Vol 125 (2) ◽  
pp. 473-480 ◽  
Author(s):  
M. A. Obaidah ◽  
K. W. Buck
Keyword(s):  
Cell Wall ◽  
Sodium Hydroxide ◽  
Periodate Oxidation ◽  
Water Soluble ◽  
Methylation Analysis ◽  
Cell Wall Polysaccharides ◽  
Partial Acid Hydrolysis ◽  
Polysaccharide Fraction ◽  
Linear Chains ◽  
Soluble Polysaccharide

1. The nature of two polysaccharides (s020 values 6S and 2S respectively in 1m-sodium hydroxide), comprising a fragment (fraction BB, [α]D +236° in 1m-sodium hydroxide), previously isolated from cell walls of Fusicoccum amygdali, has been investigated. 2. Both the major (2S) and minor (6S) components were affected by incubation with α-amylase. The 6S polysaccharide was also attacked by exo-β-(1→3)-glucanase, which is evidence that it contained both α-(1→4)- and β-(1→3)-glucopyranose linkages. By fractionation of the products of α-amylase-treated fraction BB it was possible to obtain a water-insoluble polysaccharide, fraction P ([α]D +290° in 1m-sodium hydroxide, 67% of fraction BB) and a water-soluble polysaccharide, fraction Q ([α]D +16° in 1m-sodium hydroxide, 11% of fraction BB), both of which sedimented as single boundaries with s020 values (in 1m-sodium hydroxide) of 1.7S and 4.6S respectively. 3. Evidence from periodate oxidation, methylation analysis, i.r. spectroscopy and partial acid hydrolysis showed that fraction P consisted of linear chains of α-(1→3)-glucopyranose units with blocks of one or two α-(1→4)-glucopyranose units interspersed at intervals along the main chain. The 2S polysaccharide, from which fraction P is derived, evidently also contains longer blocks of α-(1→4)-glucopyranose units, that are susceptible to α-amylase action. 4. Fraction Q consisted of glucose (88%) with small amounts of galactose, mannose and rhamnose. Evidence from digestion with exo- and endo-β-(1→3)-glucanases, periodate oxidation and methylation analysis suggests that fraction Q consists of a branched galactomannorhamnan core, to which is attached a β-(1→3)-, β-(1→6)-glucan. In the cell wall, chains of α-(1→4)-linked glucopyranose units are linked to fraction Q to form the 6S component of fraction BB.

Effect of water-soluble dietary fiber resistant dextrin on flour and bread qualities

2020 ◽  
Vol 317 ◽  
pp. 126452
Author(s):  
Zheng Huang ◽  
Jing Jing Wang ◽  
Yu Chen ◽  
Na Wei ◽  
Yi Hou ◽  
...  
Keyword(s):  
Dietary Fiber ◽  
Water Soluble ◽  
Soluble Dietary Fiber ◽  
Effect Of Water ◽  
Resistant Dextrin

scholarly journals Effects of dietary fiber on postprandial glucose in healthy adults

Mediscope ◽  
2018 ◽  
Vol 6 (1) ◽  
pp. 25-29
Author(s):  
F Yesmin ◽  
MOI Ali ◽  
MMR Sardar ◽  
MK Munna ◽  
S Baksh
Keyword(s):  
Dietary Fiber ◽  
Blood Sugar ◽  
Postprandial Glucose ◽  
Water Soluble ◽  
Controlled Clinical Trial ◽  
Oral Glucose ◽  
Soluble Dietary Fiber ◽  
Medical College ◽  
Randomized Controlled ◽  
The Mean

Frequently and persistently raised glucose level causes numerous complications. So it is important to find out a component of diet that can improve glucose homeostasis after ingestion of food without causing any side effect or complication. The aim of this randomized controlled clinical trial study was to verify the effect of dietary fiber on postprandial blood sugar (PPBS). The study was carried out in a sample of fifty healthy adult subjects at the Department Physiology of Rajshahi Medical College during the period from January 2014 to December 2014. Fasting blood sugar of the subjects was estimated before ingestion of identical glucose drink with or without dietary fiber (ispaghula husk) and PPBS was measured at 30, 60 and 120 min after the drink. The mean plasma glucose concentrations after a 50 gm oral glucose load significantly (p < 0.05, p < 0.001) decreased at 30, 60 and 120 min in both condition with dietary fiber and without dietary fiber. The mean plasma postprandial glucose concentration in condition with dietary fiber was significantly (p < 0.001) lower than the corresponding value without dietary fiber. The study suggests that ingestion of water soluble dietary fiber along with glucose drink reduces sharp rise as well as sustained rise of postprandial glucose. Mediscope Vol. 6, No. 1: Jan 2019, Page 25-29

scholarly journals Isolation and structural characterization of alkali-labile oligosaccharides from bovine milk-fat-globule membrane

1978 ◽  
Vol 171 (3) ◽  
pp. 549-557 ◽  
Author(s):  
G H Farrar ◽  
R Harrison
Keyword(s):  
Mass Spectrometry ◽  
Structural Characterization ◽  
Milk Fat ◽  
Bovine Milk ◽  
Periodate Oxidation ◽  
Methylation Analysis ◽  
Milk Fat Globule Membrane ◽  
Milk Fat Globule ◽  
Fat Globule

Treatment of sialoglycopeptides derived from bovine milk-fat-globule membrane with alkaline borohydride released a reduced oligosaccharide fraction from which a tetrasaccharide and two trisaccharides were isolated. Periodate-oxidation studies coupled with methylation analysis and g.l.c.-mass spectrometry established their structures as: N-acetylneuraminyl-(2 leads to 3)-beta-D-galactopyranosyl-(1 leads to 3)-[N-acetylneuraminyl-(2 leads to 6)]-N-acetyl-D-galactosaminitol, N-acetylneuraminyl-(2 leads to 3)-D-glactopyranosyl-(1 leads to 3)-N-acetyl-D-galactosaminitol and beta-D-galactopyranosyl-(1 leads to 3)-[N-acteylneuraminyl-(2 leads to 6)]-N-acetyl-D-galactosaminitol respectively.

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