Chemical Composition of Essential Oils of Grindelia squarrosa and G. hirsutula

The essential oils of Grindelia squarrosa (Pursh) Dunal and G. hirsutula Hook. & Arn. cultivated in Romania were isolated by hydrodistillation. The essential oils were analyzed by a combination of GC-FID and GC-MS. The identification of the constituents was achieved from their retention indices and comparison of their MS data with computer library database and literature data. The fifty-six identified constituents accounted for 72.1-81.3% of the oils. The oils were found to contain α-pinene, β-pinene, limonene, borneol, bornyl acetate and germacrene D as main constituents. The oils obtained from the two species showed small differences in chemical composition. However, menthol, menthone and pulegone were detected only in the essential oil of G. hirsutula.

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Chemical composition of the essential oils of four Polyalthia species from Malaysia

Zeitschrift für Naturforschung C ◽

10.1515/znc-2020-0097 ◽

2020 ◽

Vol 75 (11-12) ◽

pp. 473-478

Author(s):

Natasa Mohd Shakri ◽

Wan Mohd Nuzul Hakimi Wan Salleh ◽

Shamsul Khamis ◽

Nor Azah Mohamad Ali ◽

Shazlyn Milleana Shaharudin

Keyword(s):

Gas Chromatography ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Folk Medicine ◽

Principal Component ◽

Gas Chromatography Mass Spectrometry ◽

Chemical Compositions ◽

Gastrointestinal Ulcer ◽

Germacrene D

AbstractPolyalthia is one of the largest genera in the Annonaceae family, and has been widely used in folk medicine for the treatment of rheumatic fever, gastrointestinal ulcer, and generalized body pain. The present investigation reports on the extraction by hydrodistillation and the composition of the essential oils of four Polyalthia species (P. sumatrana, P. stenopetalla, P. cauliflora, and P. rumphii) growing in Malaysia. The chemical composition of these essential oils was determined by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS). The multivariate analysis was determined using principal component analysis (PCA) and hierarchical clustering analysis (HCA) methods. The results revealed that the studied essential oils are made up principally of bicyclogermacrene (18.8%), cis-calamenene (14.6%) and β-elemene (11.9%) for P. sumatrana; α-cadinol (13.0%) and δ-cadinene (10.2%) for P. stenopetalla; δ-elemene (38.1%) and β-cubebene (33.1%) for P. cauliflora; and finally germacrene D (33.3%) and bicyclogermacrene for P. rumphii. PCA score and HCA plots revealed that the essential oils were classified into three separated clusters of P. cauliflora (Cluster I), P. sumatrana (Cluster II), and P. stenopetalla, and P. rumphii (Cluster III) based on their characteristic chemical compositions. Our findings demonstrate that the essential oil could be useful for the characterization, pharmaceutical, and therapeutic applications of Polyalthia essential oil.

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Chemical Composition and Antibacterial Activity of the Essential Oil of Baccharis latifolia Pers. and B. Prunifolia H. B. &K. (Asteraceae)

Natural Product Communications ◽

10.1177/1934578x0700201211 ◽

2007 ◽

Vol 2 (12) ◽

pp. 1934578X0700201 ◽

Cited By ~ 1

Author(s):

Janne Rojas ◽

Judith Velasco ◽

Luis B. Rojas ◽

Tulia Díaz ◽

Juan Carmona ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Enterococcus Faecalis ◽

Mass Spectra ◽

Retention Indices ◽

Gram Positive ◽

Gram Positive Bacteria

The essential oils from leaves of Baccharis latifolia and B. prunifolia collected in January 2006 were analyzed by GC/MS. The yields of oils extracted by hydrodistillation were 0.27 and 0.29% for B. latifolia and B. prunifolia, respectively. Sixteen (B. latifolia) and twenty nine (B. prunifolia) components were identified by comparison of their mass spectra with the Wiley GC-MS Library data and by their retention indices (RI). The identified products may be divided into four different groups: monoterpenes (9.0% B. latifolia; 43.9% B. prunifolia), oxygenated monoterpenes (0.8% B. latifolia; 5.4% B. prunifolia), sesquiterpenes (20.4% B. latifolia; 45.9% B. prunifolia) and oxygenated sesquiterpenes (69.8% B. latifolia; 1.9% B. prunifolia). The oils showed antibacterial activity only against Gram positive bacteria, with MIC values for Staphylococcus aureus (ATCC 25923) of 80 μg/mL (B. latifolia) and Enterococcus faecalis (ATCC 29212) of 90 μg/mL and 260 μg/mL (B latifolia and B. prunifolia, respectively).

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Chemical Composition and Anti-mildew Activities of Essential Oils from Different Parts of Michelia compressa var. formosana

Natural Product Communications ◽

10.1177/1934578x1501000435 ◽

2015 ◽

Vol 10 (4) ◽

pp. 1934578X1501000

Author(s):

Yu-Chang Su ◽

Kuang-Ping Hsu ◽

Eugene I-Chen Wang ◽

Chen-Lung Ho

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Main Constituent ◽

Germacrene D ◽

Leaf Oil ◽

Essential Oil Yield ◽

Different Parts ◽

Type Apparatus

In this study, anti-mildew activities of the essential oils of the leaves, wood, twigs, flowers, and their constituents from Michelia compressa var. formosana were evaluated in vitro against 7 mildew fungi. The main compounds responsible for the anti-mildew activities were isolated and identified. The essential oils from the fresh leaves, wood, twigs, and flowers of M. compressa var. formosana were isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. The essential oil yield from the flowers was the highest, and the oils obtained from the 4 parts of the tree were composed mainly of sesquiterpenoids. The leaf oil consisted primarily of α-cadinol (18.9%) and germacrene D (18.5%); the twig oil's main constituent was α-cadinol (19.1%); the wood oil's major components were α-cadinol (25.7%) and β-eudesmol (20.2%); and the flower oil contained α-cadinol (11.4%). Comparing the anti-mildew activities of the oils suggested that wood oil was the most effective. Further fractionation of the wood oil produced α-cadinol, β-eudesmol, τ-cadinol, and elemol. The 4 compounds exhibit very strong anti-mildew activities, and these in descending order are α-cadinol, elemol, β-eudesmol, and τ-cadinol. For the anti-mildew activities of the wood oil, the active source compounds were determined to be α-cadinol, elemol, β-eudesmol, and τ-cadinol.

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Essential Oil of Piper oradendron from the Pacific Slope of Guatemala

Natural Product Communications ◽

10.1177/1934578x1901400121 ◽

2019 ◽

Vol 14 (1) ◽

pp. 1934578X1901400 ◽

Cited By ~ 1

Author(s):

José V. Martínez-Arévalo ◽

Sully M. Cruz ◽

Miriam A. Apel ◽

Amélia T. Henriques ◽

Armando Cáceres

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Endemic Species ◽

First Report ◽

The Pacific ◽

Three Samples ◽

Germacrene D ◽

Pacific Slope ◽

Main Components

Essential oils of leaves of the endemic species Piper oradendron Trel. & Standl. (Piperaceae) were obtained from three sites of the Guatemalan Pacific slope. The yields of the essential oils obtained by hydrodistillation varied from the site of collection (0.4-1.4%). The GC/MS analysis showed that sample A (Samayac) had up to 53 peaks that were identified, B (Popoyá) showed 31 peaks and C (Bulbuxyá) showed 22; major constituents were similar in the three samples, including α- and β-pinene (28.3-46.9%), germacrene D (10.7-22.7%), and iso-spathulenol (10.2-22-3%). This is the first report on the chemical composition of the essential oil of samples of P. oradendron from different provenances of Guatemala, suggesting little variability in its main components.

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Chemical Composition of Essential Oil of Amomum xanthioides Wall. ex Baker from Northern Vietnam

Biointerface Research in Applied Chemistry ◽

10.33263/briac114.1227512284 ◽

2021 ◽

Vol 11 (4) ◽

pp. 12275-12284

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Oil Content ◽

Bornyl Acetate ◽

Collection Site ◽

Fresh Weight ◽

Northern Vietnam ◽

Germacrene D ◽

Total Oil ◽

Time Of Collection

The essential oil from leaves, roots, stems, and fruits of Amomum xanthioides Wall. ex Baker from Northern Vietnam was obtained by hydrodistillation and analyzed by gas chromatography techniques. The yield of the essential oil obtained from leaves, roots, stems, and fruits of A. xanthioides was 0.26%, 0.24%, 0.19%, and 1.45% (w/w) by fresh weight, respectively. The composition of the oil samples was compared; 38, 43, 28, and 22 compounds have been identified in the essential oil of leaves, roots, stems, and fruits, accounting for 96.19%, 96.61%, 98.39%, and 98.12% of the total oil content, respectively. A high variation among organs for the majority of compounds was shown. β-elemene (31.71%), δ-cadinene (10.69%), germacrene D (9.55%), bicycloelemene (8.12%), and bicyclogermacrene (7.93%) are the main compounds in leaves, while β-pinene (29.59%), terpinen-4-ol (10.77%), and α-terpinene (6.96%) are identified as the main compounds in roots. Stems are characterized by high levels of β-elemene (29.58%), spathoulenol (26.89%), and bicycloelemene (6.19%). For fruits, bornyl acetate (37.21%), camphor (19.48%), camphene (14.62%), and limonene (9.64%) are the main compounds. Overall, this study confirms that changes in the yield, composition, and concentration of the essential oil may be caused by several factors such as environmental conditions and climate at the collection site, the time of collection, and the age and properties of plants. This study also provides new data on the chemical composition of the essential oil from A. xanthioides collected from Northern Vietnam.

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ANALYSIS OF ESSENTIAL OIL CONSTITUENTS OF THREE ANNONA SPECIES GROWING IN KERALA, INDIA

International Journal of Research in Ayurveda and Pharmacy ◽

10.7897/2277-4343.1205141 ◽

2021 ◽

Vol 12 (5) ◽

pp. 45-48

Author(s):

Sonia Mol Joseph ◽

Amala Dev A R

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Mass Spectrometry Analysis ◽

Gas Chromatography Mass Spectrometry ◽

Spectrometry Analysis ◽

South Indian ◽

Germacrene D ◽

Leaf Essential Oil ◽

The Common

The volatile chemical composition of leaf essential oils of three Annona species (Annona cherimola, Annona muricata and Annona squamosa) from the different regions of Kerala, South Indian was determined using gas chromatography-mass spectrometry analysis. A total of 41 constituents belonging to monoterpenoids, sesquiterpenoids and diterpenoids were identified. Monoterpenes and sesquiterpenoids were the major class of volatile compounds in most of the Annona species examined. The study led to the identification of major compounds as germacrene D (23.5%), bicyclogermacrene (14.6%) and β-caryophyllene (11.7%) in A. cherimola, α-pinene (13.3%), β-caryophyllene (11.2%) and β-pinene (10.1%) in A. muricata, and β-caryophyllene (11.9%) and α-pinene (8.2%) in A. squamosa respectively. The examined essential oils showed that β-caryophyllene is the common constituent identified in all the three species and other constituents are more specific for each species under study. Present investigation reports, a comparison of essential oil compositions of three Annona species from Kerala. Results of this study prove that essential oils examined have considerable dissimilarity in chemical composition with previously reported leaf essential oil compositions from other regions. A chemotaxonomic analysis of these essential oils based on the distribution of compounds has revealed an efficient method to differentiate Annona species unambiguously.

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Comparison of chemical composition of the essential oil from Marrubium vulgare L. and M. incanum Desr. during the second year of cultivation

Acta Agrobotanica ◽

10.5586/aa.2015.002 ◽

2015 ◽

Vol 68 (1) ◽

pp. 59-62 ◽

Cited By ~ 5

Author(s):

Grażyna Zawiślak

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Chromatographic Analysis ◽

Germacrene D ◽

Marrubium Vulgare ◽

Second Year ◽

Favorable Conditions

Horehound herb (Marrubium vulgare L.) is harvested from plantations in Poland. In our country, there are also favorable conditions for M. incanum Desr. growing. The aim of the study was to compare the chemical composition of essential oils from M. vulgare L. and M. incanum Desr. in the second year of cultivation. The study revealed the presence of 31 compounds in the essential oil from M. vulgare L. and 24 compounds in the essential oil from M. incanum Desr. Chromatographic analysis revealed that the main compounds in the essential oil of M. vulgare L. from a two-year plantation were as follows: E-caryophyllene (34.51–36.78%), germacrene D (22.45–27.18%), bicyclogermacrene (9.54–11.12%), δ-amorphene (6.15–8.18%), and carvacrol (4.71–6.64%), whereas the following compounds prevailed in the oil from M. incanum Desr.: germacrene D (28.75–32.14%), E-caryophyllene (23.18–29.57%), α-cadinol (13.59–20.84%), and carvacrol (2.08–7.47%).

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Chemical composition, antimicrobial activity and chromosome number of Hertia cheirifolia L. from Algeria

Acta Scientifica Naturalis ◽

10.2478/asn-2020-0018 ◽

2020 ◽

Vol 7 (2) ◽

pp. 31-43

Author(s):

Ounoughi Abdelkader ◽

Ramdani Messaoud ◽

Lograda Takia ◽

Chalard Pierre ◽

Figueredo Gilles

Keyword(s):

Antibacterial Activity ◽

Chemical Composition ◽

Chromosome Number ◽

Essential Oil ◽

Essential Oils ◽

Diffusion Method ◽

Chemical Compositions ◽

Root Cells ◽

Karyotypic Analysis ◽

Germacrene D

AbstractThe aims of this work are to investigate the chemical composition, the antibacterial activity of the essential oil and the chromosome numbers of two populations of Hertia cheirifolia. The samples were collected in the flowering stage, in eastern Algeria locality. The aerial parts of H. cheirifolia were submitted to a hydro-distillation. GC and GC / MS analysed the chemical compositions of the obtained essential oils. The antibacterial activity of essential oils was evaluated using the disks diffusion method against ten bacterial strains. For karyotypic analysis, the squashing method is used. Fifty-eight compounds representing 98.93% of the total oil were identified in H. cheirifolia. The chemical composition is dominated by the presence of major products, α-pinene (48.49 - 53.85%) and Germacrene-D (2.64 - 12.66%). Two distinct chemical breeds were identified, the α-pinene-spathulenol of Batna population, and the α-pinene-germacrene-D of Setif population. The essential oil of H. cheirifolia has a moderate activity against bacteria tested. In contrast, the strains E. coli ATCC 25922, P. syringae ATCC 53543 and E. fecalis ATCC 49452 are resistant to H. cheirifolia essential oils. The observations of root cells meristematic at metaphase of H. cheirifolia gave a diploid chromosome number 2n = 2x = 20, with a basic chromosome number (x= 10).

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Chemical composition and antifungal potential of essential oils from different aerial parts of Protium ovatum Engl

Australian Journal of Crop Science ◽

10.21475/ajcs.21.15.04.p2915 ◽

2021 ◽

pp. 570-576

Author(s):

Wendel C. de Sousa ◽

Josemar G. Oliveira Filho ◽

Cassia C. F. Alves ◽

Moacir R. Forim ◽

Cristiane de M. Cazal

Keyword(s):

Chemical Composition ◽

Antifungal Activity ◽

Essential Oil ◽

Essential Oils ◽

Antimicrobial Agents ◽

Ripe Fruit ◽

Aerial Parts ◽

Potential Source ◽

Antifungal Potential ◽

Germacrene D

Protium ovatum is a Brazilian endemic species widely distributed between the Cerrado and Amazon biomes. Here, we evaluate the chemical composition of the essential oils (EO) of different shoot organs from P. ovatum including stems, petioles, leaves, flowers, ripe and unripe fruits, and investigate their antifungal potential against Sclerotinea sclerotiorum. The EO were obtained by hydrodistillation in a Clevenger-type apparatus and analysed by GC-MS, followed by PCA analysis. The antifungal activity was performed by agar diffusion. Fruits had the highest essential oil contents among the shoot parts. The constituents were found varyingin the different organs: α-Pinene (0.80-18.3%), β-Pinene (0.58-5.17%), Myrcene (0.52-27.3%), Limonene (3.15-59.7%), Caryophyllene E (3.67-16.4%), Germacrene D (6.34-27.4%), and δ-Cadinene (2.29-7.63%). The essential oil from ripe fruit showed the strongest antifungal activity, with the highest Inhibition of Mycelial Growth (IMG) (50.11%) at the lowest concentration assayed (18.75μg.mL-1). This is the first report on the chemical composition of the essential oils from stems, petioles, flowers, and ripe fruits of P. ovatum and their antifungal activity against S. sclerotiorum, making it a potential source of antimicrobial agents

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Chemical Composition of Hypericum richeri subsp. grisebachii Essential Oil from Croatia

Natural Product Communications ◽

10.1177/1934578x1300800226 ◽

2013 ◽

Vol 8 (2) ◽

pp. 1934578X1300800

Author(s):

Igor Jerković ◽

Maja Marasović ◽

Zvonimir Marijanović ◽

Kroata Hazler Pilepić ◽

Željan Maleš ◽

...

Keyword(s):

Fatty Acids ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Decanoic Acid ◽

Chemical Profile ◽

Aerial Parts ◽

Ms Analysis ◽

Germacrene D

The aerial parts of Hypericum richeri Vill. subsp. grisebachii (Boiss.) Nyman were collected from two different locations in Croatia and subjected to hydrodistillation. GC/FID and GC/MS analysis of the isolated essential oils revealed 64 compounds representing 94.7% and 98.2% of the total oils. Predominant constituents in both samples were: germacrene D (10.9%; 6.0%), bicyclogermacrene (4.7%; 3.5%), α-pinene (6.8%; 6.9%), β-pinene (8.1%; 5.1%), decanoic acid (4.5%; 6.8%), β-caryophyllene (3.3%; 7.5%), δ-cadinene (7.0%; 4.4%), spathulenol (6.0%; 9.5%) and tetracosane (3.1%; 5.8%). Comparison of both samples revealed similarity in the chemical composition with minor fluctuations of constituent percentages. The chemical profile of Croatian oils was in general similar to those reported for other geographic areas regarding major mono- and sesquiterpene constituents. However, spathulenol, 5-cadinene and bicyclogermacrene were more abundant in Croatian oils. The presence of decanoic acid (4.5%; 6.8%) in Croatian oils was the major difference between acids and fatty acids derivatives. Higher abundance of alkanes (particularly tetracosane and docosane) was also noticed.

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