In Vitro Cytotoxic Activity Guided Essential Oil Composition of Flowering Twigs of Stevia rebaudiana

The essential oil extracted by hydrodistillation from the flowering twigs of Stevia rebaudiana Bertoni (Asteraceae) was fractioned by chromatography. Forty-three constituents were characterized with the help of GC, GC-MS and other spectroscopic techniques. The essential oil was found to be a complex mixture of mono- and sesqui-terpenes. The cytotoxicity of the essential oil and its fractions was evaluated by sulforhodamine B (SRB) based assay against two cancer cell types viz. C-6 (rat glioma cells) and CHOK1 (Chinese hamster ovary cells). The essential oil and its fractions showed promising cytotoxicity against both cell lines. The highest activity (95.6±0.6%) was show by the essential oil on the C-6 cell line at a concentration of 400 μg/mL, which was comparable with that of the standard drug vinblastin.

Download Full-text

Zephgrabetaine: A New Betaine-type Amaryllidaceae Alkaloid from Zephyranthes grandiflora

Natural Product Communications ◽

10.1177/1934578x1300800206 ◽

2013 ◽

Vol 8 (2) ◽

pp. 1934578X1300800 ◽

Cited By ~ 1

Author(s):

Deepali Katoch ◽

Dharmesh Kumar ◽

Upendra Sharma ◽

Neeraj Kumar ◽

Yogendra S. Padwad ◽

...

Keyword(s):

Cell Lines ◽

Spectroscopic Data ◽

Chinese Hamster Ovary Cells ◽

Chinese Hamster ◽

Glioma Cells ◽

Cytotoxic Activities ◽

Ovary Cells ◽

Rat Glioma ◽

Dose Dependent

Zephgrabetaine (1), a new betaine type Amaryllidaceae alkaloid, along with seven known alkaloids, lycorine, galanthine, lycoramine, hamayne, haemanthamine, tortuosine, and ungeremine were isolated from the bulbs of Zephyranthes grandiflora and their structures elucidated by spectroscopic data analysis. The isolated alkaloids were tested for in vitro cytotoxic activities against two cell lines, C-6 (rat glioma cells) and CHO-K1 (Chinese hamster ovary cells). A dose dependent cytotoxic effect was exhibited by all the alkaloids on these two cancer cell lines with prominent activity of lycorine and haemanthamine.

Download Full-text

Chemical Composition andIn VitroCytotoxic Activity of Essential Oil of Leaves ofMalus domesticaGrowing in Western Himalaya (India)

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/649727 ◽

2012 ◽

Vol 2012 ◽

pp. 1-6 ◽

Cited By ~ 13

Author(s):

Mayanka Walia ◽

Tavleen S. Mann ◽

Dharmesh Kumar ◽

Vijai K. Agnihotri ◽

Bikram Singh

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Cell Lines ◽

Chinese Hamster Ovary Cells ◽

Leukemia Cell ◽

Chinese Hamster ◽

Complex Mixture ◽

Western Himalaya ◽

Acute Monocytic Leukemia ◽

Monocytic Leukemia

Light pale-colored volatile oil was obtained from fresh leaves ofMalus domesticatree, growing in Dhauladhar range of Himalaya (Himachal Pradesh, India), with characteristic eucalyptol dominant fragrance. The oil was found to be a complex mixture of mono-, sesqui-, di-terpenes, phenolics, and aliphatic hydrocarbons. Seventeen compounds accounting for nearly 95.3% of the oil were characterized with the help of capillary GC, GC-MS, and NMR. Major compounds of the oil were characterized as eucalyptol (43.7%), phytol (11.5%), α-farnesene (9.6%), and pentacosane (7.6%). Cytotoxicity of essential oil of leaves ofM. domesticawas evaluated by sulforhodamine B (SRB) assays. The essential oil of leaves ofM. domestica, tested against three cancer cell lines, namely, C-6 (glioma cells), A549 (human lung carcinoma), CHOK1 (Chinese hamster ovary cells), and THP-1 (human acute monocytic leukemia cell). The highest activity showed by essential oil on C-6 cell lines (98.2%) at concentration of 2000 μg/ml compared to control. It is the first paper in literature to exploit the chemical composition and cytotoxic activity of leaves essential oil ofM. domestica.

Download Full-text

Design, Synthesis, Molecular Docking and Biological Evaluation of 1-(benzo[d]thiazol-2-ylamino)(phenyl)methyl)naphthalen-2-ol Derivatives as Antiproliferative Agents

Letters in Organic Chemistry ◽

10.2174/1570178616666190408101233 ◽

2019 ◽

Vol 16 (10) ◽

pp. 837-845

Author(s):

Sandhya Jonnala ◽

Bhaskar Nameta ◽

Murthy Chavali ◽

Rajashaker Bantu ◽

Pallavi Choudante ◽

...

Keyword(s):

Molecular Docking ◽

Inhibitory Activity ◽

Chinese Hamster Ovary Cells ◽

Chinese Hamster ◽

Mouse Skin ◽

Coupling Reaction ◽

Binding Mode ◽

Biological Evaluation ◽

Design Synthesis

A class of 1-((benzo[d]thiazol-2-ylamino)(phenyl)methyl)naphthalen-2-ol derivatives (4a-t) has been synthesized in good yields through a three component coupling reaction. The newly synthesized compounds were evaluated for their in vitro antiproliferative activity against five cell lines such as DU145 (human prostate cancer), MDA-MB-B231 (human breast cancer), SKOV3 (human ovarian cancer), B16-F10 (mouse skin melanoma) and CHO-K1 (Chinese hamster ovary cells), a noncancerous cell line. In vitro inhibitory activity indicates that compounds 4a, 4b, 4c, 4d, 4g, 4j, and 4o exhibited potent anti-proliferative behavior. Among them, compounds 4g, 4j and 4o found to be the most active members exhibiting remarkable growth inhibitory activity. Molecular docking facilitates to investigate the probable binding mode and key active site interactions in tubulins α and β proteins. The docking results are complementary to experimental results.

Download Full-text

Evaluation of Fab and F(ab′)2 Fragments and Isotype Variants of a Recombinant Human Monoclonal Antibody against Shiga Toxin 2

Infection and Immunity ◽

10.1128/iai.00867-09 ◽

2010 ◽

Vol 78 (3) ◽

pp. 1376-1382 ◽

Cited By ~ 17

Author(s):

Donna E. Akiyoshi ◽

Abhineet S. Sheoran ◽

Curtis M. Rich ◽

L. Richard ◽

Susan Chapman-Bonofiglio ◽

...

Keyword(s):

Monoclonal Antibody ◽

Shiga Toxin ◽

Chinese Hamster Ovary Cells ◽

Human Monoclonal Antibody ◽

Chinese Hamster ◽

The Body ◽

Neutralization Activity ◽

Shiga Toxin 2

ABSTRACT 5C12 HuMAb is a human monoclonal antibody against the A subunit of Shiga toxin 2 (Stx2). We have previously shown that 5C12 HuMAb effectively neutralizes the cytotoxic effects of this toxin by redirecting its transport within the cell and also by neutralizing the toxin's ability to inhibit protein synthesis. The 5C12 HuMAb and its recombinant IgG1 version protect mice at a dose of 0.6 μg against a lethal challenge of Stx2. The contribution of the Fc region to this observed neutralization activity of the 5C12 antibody against Stx2 was investigated in this study. Using recombinant DNA technology, 5C12 isotype variants (IgG1, IgG2, IgG3, and IgG4) and antibody fragments [Fab, F(ab′)2] were expressed in Chinese hamster ovary cells and evaluated in vitro and in vivo. All four 5C12 isotype variants showed protection in vitro, with the IgG3 and IgG4 variants showing the highest protection in vivo. The Fab and F(ab′)2 fragments also showed protection in vitro but no protection in the mouse toxicity model. Similar results were obtained for a second HuMAb (5H8) against the B subunit of Stx2. The data suggest the importance of the Fc region for neutralization activity, but it is not clear if this is related to the stability of the full-length antibody or if the Fc region is required for effective elimination of the toxin from the body.

Download Full-text

ESSENTIAL OIL COMPOSITION AND IN VITRO ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF THYMUS CAPPADOCICUS BOISS.

Journal of Food Processing and Preservation ◽

10.1111/j.1745-4549.2012.00694.x ◽

2012 ◽

Vol 37 (5) ◽

pp. 605-614 ◽

Cited By ~ 10

Author(s):

SEVIL ALBAYRAK ◽

AHMET AKSOY

Keyword(s):

Essential Oil ◽

Antimicrobial Activities ◽

Essential Oil Composition ◽

Oil Composition ◽

Antioxidant And Antimicrobial Activities

Download Full-text

Peptide Antagonists That Inhibit Sin Nombre Virus and Hantaan Virus Entry through the β3-Integrin Receptor

Journal of Virology ◽

10.1128/jvi.79.12.7319-7326.2005 ◽

2005 ◽

Vol 79 (12) ◽

pp. 7319-7326 ◽

Cited By ~ 35

Author(s):

Richard S. Larson ◽

David C. Brown ◽

Chunyan Ye ◽

Brian Hjelle

Keyword(s):

Viral Entry ◽

Sequence Similarity ◽

Chinese Hamster Ovary Cells ◽

Chinese Hamster ◽

Phage Display Library ◽

Hantaan Virus ◽

Sin Nombre Virus ◽

Fab Fragment ◽

Ovary Cells

ABSTRACT Specific therapy is not available for the treatment of hantavirus cardiopulmonary syndrome caused by Sin Nombre virus (SNV). The entry of pathogenic hantaviruses into susceptible human cells is dependent upon expression of the αvβ3 integrin, and transfection of human β3 integrin is sufficient to confer infectibility onto CHO (Chinese hamster ovary) cells. Furthermore, pretreatment of susceptible cells with anti-β3 antibodies such as c7E3 or its Fab fragment ReoPro prevents hantavirus entry. By using repeated selection of a cyclic nonamer peptide phage display library on purified αvβ3, we identified 70 peptides that were competitively eluted with ReoPro. Each of these peptides was examined for its ability to reduce the number of foci of SNV strain SN77734 in a fluorescence-based focus reduction assay according to the method of Gavrilovskaya et al. (I. N. Gavrilovskaya, M. Shepley, R. Shaw, M. H. Ginsberg, and E. R. Mackow, Proc. Natl. Acad. Sci. USA 95:7074-7079, 1998). We found that 11 peptides reduced the number of foci to a greater extent than did 80 μg/ml ReoPro when preincubated with Vero E6 cells. In addition, 8 of the 70 peptides had sequence similarity to SNV glycoproteins. We compared all 18 peptide sequences (10 most potent, 7 peptides with sequence similarity to hantavirus glycoproteins, and 1 peptide that was in the group that displayed the greatest potency and had significant sequence similarity) for their abilities to inhibit SNV, Hantaan virus (HTNV), and Prospect Hill virus (PHV) infection. There was a marked trend for the peptides to inhibit SNV and HTNV to a greater extent than they inhibited PHV, a finding that supports the contention that SNV and HTNV use β3 integrins and PHV uses a different receptor, β1 integrin. We then chemically synthesized the four peptides that showed the greatest ability to neutralize SNV. These peptides inhibited viral entry in vitro as free peptides outside of the context of a phage. Some combinations of peptides proved more inhibitory than did individual peptides. In all, we have identified novel peptides that inhibit entry by SNV and HTNV via β3 integrins and that can be used as lead compounds for further structural optimization and consequent enhancement of activity.

Download Full-text

Investigation of the Essential Oil of Ormenis africana from Morocco: Revision of Chemical Composition and Antibacterial Activity

Universities' Journal of Phytochemistry and Ayurvedic Heights ◽

10.51129/ujpah-june2021-30-1(5) ◽

2021 ◽

Vol 1 (30) ◽

pp. 34-38

Author(s):

El Hanbali F Barrero A.F

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Essential Oil Composition ◽

Proteus Vulgaris ◽

Oil Composition ◽

Gram Negative ◽

Main Compound ◽

Oxygenated Compounds ◽

Aerial Parts

Abstract- The essential oil composition from the aerial parts of Ormenis africana (Asteraceae), an endemic species from Morocco, has been investigated by GC/MS. A total of 31 compounds were identified, representing 77%. After fractionation by column chromatography, the main compound was isolated and its structure elucidated by NMR spectroscopy. The essential oil was dominated by oxygenated compounds with spathulenol (45.8%) followed by camphor (7.1%), -cadinol (5.9%) and -bisabolol (5.9%) as the main compounds. This oil can be classified as spathulenol-type according to its spathulenol content. In vitro the antibacterial activity of the whole essential oil against three Gram positive (Bacillus cereus, Enterococcus faecalis, Streptococcus C) bacteria and three Gram negative (Proteus vulgaris, Escherichia coli, Pseudomonas aeroginosa) bacteria, showed significant results. Keywords: Asteraceae, Ormenis africana, Essential oil, Spathulenol, Antibacterial activity.

Download Full-text

Hipotiroidismo Associado a Anticorpos Anti-Receptor da Hormona TSH com Ação Bloqueadora Determinada In Vitro

Acta Médica Portuguesa ◽

10.20344/amp.6521 ◽

2015 ◽

Vol 28 (5) ◽

pp. 663

Author(s):

Pedro Marques ◽

Karim Chikh ◽

Anne Charrié ◽

Rosa Pina ◽

Maria João Bugalho ◽

...

Keyword(s):

Hormone Receptor ◽

Chinese Hamster Ovary Cells ◽

Chinese Hamster ◽

Thyroid Stimulating Hormone ◽

Human Thyroid ◽

In Vitro Tests ◽

Lymphocytic Thyroiditis ◽

Blocking Activity ◽

Stimulating Hormone

Thyroid-stimulating hormone-receptor autoantibodies normally causes hyperthyroidism. However, they might have blocking activity causing hypothyroidism. A 11-year-old girl followed due to type 1 diabetes mellitus, celiac disease and euthyroid lymphocytic thyroiditis at diagnosis. Two years after the initial evaluation, thyroid-stimulating hormone was suppressed with normal free T4; nine months later, a biochemical evolution to hypothyroidism with thyroid-stimulating hormone-receptor autoantibodies elevation was seen; the patient remained always asymptomatic. Chinese hamster ovary cells were transfected with the recombinant human thyroid-stimulating hormone -receptor, and then exposed to the patient´s serum; it was estimated a ‘moderate’ blocking activity of these thyroid-stimulating hormone-receptor autoantibodies, and concomitantly excluded stimulating action. In this case, the acknowledgment of the blocking activity of the serum thyroid-stimulating hormone-receptor autoantibodies, supported the hypothesis of a multifactorial aetiology of the hypothyroidism, which in the absence of the in vitro tests, we would consider only as a consequence of the destructive process associated to lymphocytic thyroiditis.

Download Full-text