scholarly journals Reactive Nitrogen Species Scavenging Capacity of Aqueous and Ethanolic Extracts from Galinsoga parviflora and G. quadriradiata Herbs

2015 ◽  
Vol 10 (11) ◽  
pp. 1934578X1501001
Author(s):  
Marta Rogowska ◽  
Siniša Srečec ◽  
Agnieszka Bazylko

The aim of the study was to determine the scavenging capacity of aqueous and ethanolic extracts derived from the herb of two species of Galinsoga against NO and ONOO-. In both tests the aqueous extracts of both Galinsoga species were more active than the ethanolic extracts. The highest scavenging activity against NO, and also against ONOO- was shown by G. quadriradiata aqueous extract (SC50±SD=1.54±0.32, and 2.10±1.98, respectively). The study showed that the activity of the extracts from both Galinsoga species is related not only to the presence of flavonoids but also to caffeic acid derivatives and caffeic acid. The results of this study partly explain the beneficial effects of Galinsoga herb extracts in the treatment of skin diseases.

Antioxidants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 412 ◽  
Author(s):  
Agnieszka Tajner-Czopek ◽  
Mateusz Gertchen ◽  
Elżbieta Rytel ◽  
Agnieszka Kita ◽  
Alicja Z. Kucharska ◽  
...  

Recently, there has been increasing interest in medicinal plants, due to their content of health-promoting compounds, e.g., caffeic acids derivatives. Hence, the aim of this work was to study the antioxidant activity of extracts obtained from the following medicinal plants: caraway (Carum carvi L.), coltsfoot (Tussilago farfara L.), dandelion (Taraxacum officinale F.H.Wigg.), lovage (Levisticum officinale L.), tarragon (Artemisia dracunculus L.) and white mulberry (Morus alba L.), characterized by their high content of caffeic acid derivatives. The water-ethanolic extracts were characterized on average by about 9 times higher contents of caffeic acid derivatives level than water extracts. Both in water and water-ethanolic extracts, the dominant phenolic acid was 5-CQA (5-O-caffeoylquinic acid) and 3,4-diCQA (3,4-dicaffeoylquinic acid), then CCA-1 (chicoric acid isomer 1), which appeared only in water-ethanolic extracts. Extracts from dandelion contained compounds such as CTA (caftaric acid), CCA-1 (chicoric acid isomer 1) and CCA-2 (chicoric acid isomer 2), which were not detected in other plant extracts examined in this work. The water-ethanolic extracts from coltsfoot and tarragon were characterized by a high content of di-caffeoylquinic acids, especially 3,4-diCQA and 3,5-diCQA, respectively. It has been stated that there is a positive correlation between caffeic acid derivatives and antioxidant activity (radical cation scavenging activity (ABTS) and radical scavenging activity (DPPH)), especially in water-ethanolic extract of medicinal plants.


Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 5142
Author(s):  
Plamen Momchev ◽  
Petar Ciganović ◽  
Mario Jug ◽  
Eva Marguí ◽  
Jasna Jablan ◽  
...  

Echinacea purpurea is used in herbal medicinal products for the prevention and treatment of the common cold, as well as for skin disorders and minor wounds. In this study, the efficiency of traditional maceration using water and ethanol was compared with the maceration using mixtures of water and glycerol, a non-toxic, biodegradable solvent from renewable sources. It was found that the glycerol–water mixtures were as effective as ethanol/water mixtures for the extraction of caffeic acid derivatives. All the prepared extracts demonstrated notable antiradical properties. Furthermore, an efficient ultrasound-assisted extraction using glycerol–water mixtures was developed using six independent variables. Their levels needed for the maximum extraction of caffeic acid derivatives were as follows: glycerol 90% (m/m), temperature 70 °C, ultrasound power 72 W, time 40 min, and ascorbic acid 0 mg/mL. Under the optimized conditions, ultrasound-assisted extraction was superior to maceration. It achieved significantly higher yields of phenolic acids in shorter extraction time. The presence of zinc in plant material may contribute to the beneficial effects of E. purpurea preparations. Since glycerol is a non-toxic solvent with humectant properties, the prepared extracts can be directly used for the preparation of cosmetics or oral pharmaceutical formulations without the need for solvent removal.


2019 ◽  
Vol 41.2 ◽  
pp. 6903-6915
Author(s):  
SARAKA Allou Isidore ◽  
ABO Kouabenan ◽  
OUATTARA Katinan Etienne ◽  
ZIRIHI Guédé Noël

L’objectif de ce travail est d’évaluer le pouvoir fongicide des feuilles de Mallotus oppositifolius sur Phytophthora sp. et Fusarium sp. deux champignons phytopathogènes dans le cadre de la recherche de substances naturelles d’origine végétale comme alternative aux pesticides chimiques en agriculture. Une enquête ethnobotanique réalisée auprès de quarante tradipraticiens de santé du District de Yamoussoukro sur les Euphorbiaceae médicinales a permis d’identifier treize espèces. L’étude a montré que les maladies de la peau (36%) étaient majoritairement traitées par ces Euphorbiaceae dont Mallotus oppositifolius. Au vue de ces données et celles de la littérature, Mallotus oppositifolius a été retenu pour l’évaluation du potentiel antifongique contre Fusarium sp. et Phytophthora sp. L'activité antifongique a été évaluée par la méthode de double dilution sur milieu PDA avec des extraits éthanoliques et aqueux dérivés des feuilles de cette plante. Les résultats ont montré que les extraits aqueux et éthanoliques 70% de Mallotus oppositifolius possèdent des propriétés antifongiques à la fois sur Fusarium sp. et Phytophthora sp. Les activités antifongiques ont été plus importantes avec les extraits éthanoliques qu’avec les extraits aqueux. Avec une concentration minimal inhibitrice CMI = 25±0,0 mg/mL, une CI50 = 6,5±0,05 mg/mL et une concentration minimal fongicide CMF = 200±0,0 mg/mL Fusarium sp. semble être le plus sensible. Phytophthora sp. est moins sensible avec une CMI = 50±0,0 mg/mL, une CI50 = 12±0,05 mg/mL et une CMF = 200±0,0 mg/mL. Le screening phytochimique a mis en évidence la présence de sept groupes de composés parmi lesquels les alcaloïdes, les polyterpènes et stérols, les tanins galliques et les catéchines sont les plus abondants dans les extraits éthanoliques. En plus de ces composés, les flavonoïdes, les polyphénols ainsi que les saponines sont aussi bien présents dans les extraits aqueux que dans les extraits alcooliques de Mallotus oppositifolius. Ces composés pourraient justifier l’activité des extraits éthanoliques de Mallotus oppositifolius. Ces résultats peuvent servir pour les recherches agronomiques dans le cadre de la lutte contre les deux champignons phytopathogènes étudiés. Botanical study, phytochemical screening and in vitro evaluation of the antifungal activity of Mallotus oppositifolius (Geisel.) Müll. Arg. (Euphorbiaceae) leaf extracts on Fusarium sp. and Phytophthora sp., two phytopathogenic fungi. ABSTRACT The purpose of this work is to evaluate the fungicidal power of the leaves of Mallotus oppositifolius on Phytophthora sp. and Fusarium sp. two phytopathogenic fungi in the research of natural substances of plant origin as an alternative to chemical pesticides in agriculture. An ethnobotanical survey of forty traditional healers in the Yamoussoukro District on medicinal Euphorbiaceae identified thirteen species.The study showed that skin diseases (36%) were mainly treated by these Euphorbiaceae including Mallotus oppositifolius. In view of these data and those in the literature, Mallotus oppositifolius was selected for the evaluation of the antifungal potential against Fusarium sp. and Phytophthora sp. The antifungal activity was evaluated by the double dilution method on PDA medium with ethanolic and aqueous extracts derived from the leaves of this plant. The results showed that aqueous and ethanolic extracts 70% of Mallotus oppositifolius have antifungal properties on both Fusarium sp. and Phytophthora sp. Antifungal activities were more important with ethanolic extracts than with aqueous extracts. With a minimum inhibitory concentration MIC = 25±0.0 mg/mL, a minimal concentration for 50 % of inhibition IC50 = 6.5±0.05 mg/mL and a minimum fungicide concentration MFC = 200±0.0 mg/mL Fusarium sp. appears to be the most sensitive. Phytophthora sp. is less sensitive with an MIC = 50±0.0 mg/mL, an IC50 = 12±0.05 mg/mL and a MFC = 200±0.0 mg/mL. Phytochemical screening revealed the presence of seven groups of compounds among which alkaloids, polyterpenes and sterols, gallic tannins and catechins are the most abundant in ethanol extracts. In addition to these compounds, flavonoids, polyphenols and saponins are present in both aqueous and alcoholic extracts of Mallotus oppositifolius. These compounds could justify the activity of the ethanolic extracts of Mallotus oppositifolius. These results can be used for agronomic research to control the two phytopathogenic fungi studied.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Fatemeh Ahmadi ◽  
Abbas Samadi ◽  
Ebrahim Sepehr ◽  
Amir Rahimi ◽  
Sergey Shabala

AbstractMedicinal plants are considered as one of the most important sources of chemical compounds, so preparing a suitable culture media for medicinal plant growth is a critical factor. The present study is aimed to improve the caffeic acid derivatives and alkylamides percentages of Echinacea purpurea root extract in hydroponic culture media with different perlite particle size and NO3−/NH4+ ratios. Perlite particle size in the growing media was varied as very coarse perlite (more than 2 mm), coarse perlite (1.5–2 mm), medium perlite (1–1.5 mm), fine perlite (0.5–1 mm), and very fine perlite (less than 0.5 mm) in different ratios to peat moss (including pure perlite, 50:50 v/v, 30:70 v/v, and pure peat moss). Two NO3−/NH4+ ratios (90:10 and 70:30) were tested in each growing media. All phytochemical analyses were performed according to standard methods using high performance liquid chromatography (HPLC). It was found that the E. purpurea grown in the medium containing very fine-grade perlite with 50:50 v/v perlite to peat moss ratio had the maximum caffeic acid derivatives, including chicoric acid (17 mg g−1 DW), caftaric acid (6.3 mg g−1 DW), chlorogenic acid (0.93 mg g−1 DW), cynarin (0.84 mg g−1 DW), and echinacoside (0.73 mg g−1 DW), as well as, alkylamides (54.21%). The percentages of these phytochemical compounds increased by decreasing perlite particle size and increasing of NO3−/NH4+ ratio. The major alkylamide in the E. purpurea root extract was dodeca-2E, 4E, 8Z-10 (E/Z)-tetraenoic acid isobutylamide in all treatments, ranging from 31.12 to 54.21% of total dry weight. It can be concluded that optimizing hydroponic culture media and nutrient solution has significant effects on E. purpurea chemical compounds.


Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 624
Author(s):  
Laima Česonienė ◽  
Juozas Labokas ◽  
Ina Jasutienė ◽  
Antanas Šarkinas ◽  
Vilma Kaškonienė ◽  
...  

The aim of the study was to evaluate 11 cultivars of blue honeysuckle (Lonicera caerulea L.) for bioactive compounds, antioxidant capacity, and the antibacterial activity of berries. Total phenolic contents (TPCs) and total anthocyanin contents (TACs) were established by using ethanolic extracts. For contents of organic acids and saccharides, aqueous extracts were used, and vitamin C was determined by using oxalic acid solution. DPPH• radical scavenging capacity was evaluated by using ethanolic extracts; antibacterial activity was assessed by using both ethanolic and aqueous extracts. The TPC varied from 364.02 ± 0.41 mg/100 g in ‘Vostorg’ to 784.5 ± 0.3 mg/100 g in ‘Obilnaja’, and TAC ranged from 277.8 ± 1.1 mg/100 g in ‘Čelnočnaja’ to 394.1 ± 8.4 mg/100 g in ‘Nimfa’. Anthocyanins comprised 53.8% of total phenolic contents on average. Among organic acids, citric acid was predominant, averaging 769.41 ± 5.34 mg/100 g, with malic and quinic acids amounting to 289.90 ± 2.64 and 45.00 ± 0.37 mg/100 g on average, respectively. Contents of vitamin C were 34.26 ± 0.25 mg/100 g on average. Organic acids were most effective in the inhibition of both Gram-positive and Gram-negative bacteria tested. In conclusion, berries of L. caerulea are beneficial not only for fresh consumption, but also as a raw material or ingredients of foods with high health-promoting value.


Foods ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 59
Author(s):  
Janusz Malarz ◽  
Klaudia Michalska ◽  
Anna Stojakowska

The objective of the present study was to characterize chemical composition of hitherto unexamined aerial parts of Lactuca sativa var. angustana cv. Grüner Stern. In contrast to leafy and head varieties of the lettuces, asparagus lettuce grown in Europe is much less studied. Fractionation of a methanolic extract from leaves of L. sativa cv. Grüner Stern, supported with HPLC/DAD and 1H NMR analysis, led to the isolation and/or identification of numerous terpenoid and phenolic compounds, including five apocarotenoids—(-)-loliolide, (+)-dehydrovomifoliol, blumenol A, (6S,9S)-vomifoliol, and corchoionoside C; three sesquiterpene lactones; two lignans—((+)-syringaresinol and its 4-O-β-glucoside); five caffeic acid derivatives; and three flavonoids. Some of the compounds, to the best of our knowledge, have never been isolated from L. sativa before. Moreover, monolignols, phenolic acids and a tryptophan-derived alkaloid were found in the analyzed plant material. Stems, leaves and shoot tips of the asparagus lettuce were examined to assess their phenolics and sesquiterpene lactone content as well as DPPH scavenging activity. Another stem lettuce—L. sativa var. angustana cv. Karola, two cultivars of leafy lettuces and one species of wild lettuce—L. serriola, were also examined as a reference material using HPLC/DAD. The results have been discussed regarding our previous studies and the literature data available.


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