scholarly journals A New Flavonoid Glycoside from Lysionotus pauciflorus

2016 ◽  
Vol 11 (5) ◽  
pp. 1934578X1601100
Author(s):  
Wei Luo ◽  
Yaya Wen ◽  
Yanbei Tu ◽  
Hongjian Du ◽  
Qin Li ◽  
...  
Keyword(s):  
Flavonoid Glycoside ◽  
Spectroscopic Methods ◽  
Phenylpropanoid Glycoside ◽  
The Family ◽  
First Time

Ten flavonoids (1–10), including a new glycoside (nevadensin-7-sambubioside, 7), together with a phenylpropanoid glycoside (11) were isolated from Lysionotus pauciflorus. Their structures were elucidated by a combination of spectroscopic methods and comparing with literature data. Five compounds (1, 3, 4, 8, and 9) were obtained from the family Gesneriaceae for the first time. The new compound was evaluated in vitro for anticholinesterase activities against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), but was found to be inactive.

Chemical components of the rhizomes of Drynaria fortunei (KUNZE) J. Sm. (polypodiaceae) in Vietnam

2011 ◽  
Vol 76 (9) ◽  
pp. 1133-1139 ◽  
Author(s):  
Pham Thi Nhat Trinh ◽  
Nguyen Cong Hao ◽  
Phan Thanh Thao ◽  
Le Tien Dung
Keyword(s):  
Data Analysis ◽  
Spectral Data ◽  
Phenolic Acid ◽  
Ethanol Extract ◽  
Chemical Components ◽  
Spectroscopic Methods ◽  
Phenylpropanoid Glycoside ◽  
Spectral Data Analysis ◽  
First Time

From the ethanol extract of Drynaria fortunei (KUNZE) J. Sm., a new phenylpropanoid glycoside, fortunamide (1), was isolated and characterized by spectroscopic methods. Together with a new glycoside, 9 known compounds, including three curcuminoids (2–4), two isoprenylated flavonoids (5, 6), two flavonoids (7, 8), one monoterpenoid (9) and one phenolic acid (10) were isolated and identified by spectral data analysis from the rhizomes of Drynaria fortunei (KUNZE) J. Sm. Eight of them were isolated from Drynaria fortunei (KUNZE) J. Sm. for the first time.

Isoflavones from Camphorosma lessingii Inhibit the Organic Anion Transporters OAT1 and OAT3

Planta Medica ◽  
2018 ◽  
Vol 85 (03) ◽  
pp. 225-230 ◽  
Author(s):  
Xinhui Wang ◽  
Dujuan Wang ◽  
Xue Wang ◽  
Manana Khutsishvili ◽  
Kamilla Tamanyan ◽  
...  
Keyword(s):  
Organic Anion ◽  
Inhibitory Effect ◽  
Spectroscopic Methods ◽  
Organic Anion Transporters ◽  
One Dimensional ◽  
Anion Transporters ◽  
Significant Inhibitory Effect ◽  
Phytochemical Investigation ◽  
The Family ◽  
First Time

AbstractPhytochemical investigation of Camphorosma lessingii has resulted in the isolation of four previously unreported isoflavones (1–4) and eight known compounds (5–12). Nine of these compounds (1–6, 8–10) are reported for the first time from members of the family Amaranthaceae. The structures of all isolated compounds were determined by spectroscopic methods, primarily one-dimensional and two-dimensional nuclear magnetic resonance and mass spectrometry. The absolute configuration of 6 was confirmed by circular dichroism. Inhibition of the organic anion transporters, OAT1 and OAT3, by the isolated compounds was evaluated. Among them, 7, 2′-dihydroxy- 6,8-dimethoxyisoflavone (1), 2′-hydroxy-6,7,8-trimethoxyisoflavone (2), 6,2′-dihydroxy-7,8-dimethoxyisoflavone (3), and 7-methoxyflavone (5) showed a significant inhibitory effect on 6-carboxyfluorescein uptake mediated by OAT1 and OAT3.

scholarly journals The Relaxase of the Rhizobium etli Symbiotic Plasmid Shows nic Site cis-Acting Preference

2006 ◽  
Vol 188 (21) ◽  
pp. 7488-7499 ◽  
Author(s):  
Daniel Pérez-Mendoza ◽  
María Lucas ◽  
Socorro Muñoz ◽  
José A. Herrera-Cervera ◽  
José Olivares ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Rhizobium Etli ◽  
Cis Acting ◽  
Cleavage Activity ◽  
Symbiotic Plasmid ◽  
The Family ◽  
Nick Site ◽  
First Time

ABSTRACT Genetic and biochemical characterization of TraA, the relaxase of symbiotic plasmid pRetCFN42d from Rhizobium etli, is described. After purifying the relaxase domain (N265TraA), we demonstrated nic binding and cleavage activity in vitro and thus characterized for the first time the nick site (nic) of a plasmid in the family Rhizobiaceae. We studied the range of N265TraA relaxase specificity in vitro by testing different oligonucleotides in binding and nicking assays. In addition, the ability of pRetCFN42d to mobilize different Rhizobiaceae plasmid origins of transfer (oriT) was examined. Data obtained with these approaches allowed us to establish functional and phylogenetic relationships between different plasmids of this family. Our results suggest novel characteristics of the R. etli pSym relaxase for previously described conjugative systems, with emphasis on the oriT cis-acting preference of this enzyme and its possible biological relevance.

scholarly journals Antileishmanial Phenylpropanoids from the Leaves ofHyptis pectinata(L.) Poit

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Rosangela A. Falcao ◽  
Patricia L. A. do Nascimento ◽  
Silvana A. de Souza ◽  
Telma M. G. da Silva ◽  
Aline C. de Queiroz ◽  
...  
Keyword(s):  
Rosmarinic Acid ◽  
Bacterial Infections ◽  
Ethanol Extract ◽  
Spectroscopic Methods ◽  
Etoh Extract ◽  
Promastigote Form ◽  
Water Fractions ◽  
New Compounds ◽  
First Time

Hyptis pectinata, popularly known in Brazil as “sambacaitá” or “canudinho,” is an aromatic shrub largely grown in the northeast of Brazil. The leaves and bark are used in an infusion for the treatment of throat and skin inflammations, bacterial infections, pain, and cancer. Analogues of rosmarinic acid and flavonoids were obtained from the leaves ofHyptis pectinataand consisted of two new compounds, sambacaitaric acid (1) and 3-O-methyl-sambacaitaric acid (2), and nine known compounds, rosmarinic acid (3), 3-O-methyl-rosmarinic acid (4), ethyl caffeate (5), nepetoidin A (6), nepetoidin B (7), cirsiliol (8), circimaritin (9), 7-O-methylluteolin (10), and genkwanin (11). The structures of these compounds were determined by spectroscopic methods. Compounds1–5, and7were evaluatedin vitroagainst the promastigote form ofL. braziliensis, and the ethanol extract. The hexane, ethyl acetate, and methanol-water fractions were also evaluated. The EtOH extract, the hexane extract, EtOAc, MeOH:H2O fractions; and compounds1,2and4exhibited antileishmanial activity, and compound1was as potent as pentamidine. In contrast, compounds3,5, and7did not present activity against the promastigote form ofL. braziliensisbelow 100 µM. To our knowledge, compounds1and2are being described for the first time.

scholarly journals An Assessment of Major Nutritional Components and Some Secondary Metabolites of In-Vitro Propagated Stevia Rebaudiana (Cultured in Bangladesh) Plant Leaves Dry Powder

2015 ◽  
Vol 3 (4) ◽  
pp. 721-726
Author(s):  
Md. Moinul Abedin Shuvo ◽  
Mohammad AL - Mamun ◽  
Tuhina Chowdhury ◽  
Nurul Absar ◽  
Md. Hasanuzzaman
Keyword(s):  
Secondary Metabolites ◽  
Stevia Rebaudiana ◽  
Plant Leaves ◽  
Dry Powder ◽  
Total Carbohydrate ◽  
Chlorophyll Contents ◽  
The Family ◽  
Chlorophyll A And B ◽  
First Time

Stevia rebaudiana, belongs to the family of Asteraceae, is a perennial and medicinal shrub. It’s leaves dry powder widely used as a natural sweetener which has no calories but reported to be 50– 300 times sweetener as sweet as sugar, that are said to be having insulin balancing properties without any side effects. This research was first time conducted in Bangladesh where a quantitative analysis were performed for determining the major constituents of in vitro propagated Stevia rebaudiana plant leaves dry powder that was prepared through oven dry after sun dry. The analysis revealed the powder as mild acidic (PH 5.345). Total chlorophyll contents were found to be 0.845mg% where chlorophyll–a and -b were 0.088 and 0.761 mg% respectively.  The major macro nutrients such as ash, crude fiber, total carbohydrate, total protein and fat were determined at the amount of 7.05, 10.5, 53.52, 13.13 and 3.55 gm% respectively where the micro nutrients like Iron (Fe), Sodium (Na), Potassium (K), Calcium (Ca), Magnesium (Mg), Phosphorus (P) and Chloride (Cl) were found at the amount of 34.2, 184.3, 2500, 534.43, 465.34, 304.7 and 49.6 mg% accordingly.  Screening for secondary metabolites showed the presence of alkaloids, flavonoids, saponins and tannins as the amount of 2.83, 17.20, 6.69 and 5.84 mg% respectively in dry powder. These findings also have been compared with the other reported values on Stevia from different countries of the world.   Int J Appl Sci Biotechnol, Vol 3(4): 721-726

scholarly journals Lausannevirus Encodes a Functional Dihydrofolate Reductase Susceptible to Proguanil

2017 ◽  
Vol 61 (4) ◽  
Author(s):  
L. Mueller ◽  
P. M. Hauser ◽  
F. Gauye ◽  
G. Greub
Keyword(s):  
Dihydrofolate Reductase ◽  
Expression System ◽  
Open Reading Frames ◽  
Dna Viruses ◽  
Content Type ◽  
The Family ◽  
Nucleocytoplasmic Large Dna Viruses ◽  
First Time ◽  
Reading Frames

ABSTRACT Lausannevirus belongs to the family Marseilleviridae within the group of nucleocytoplasmic large DNA viruses (NCLDVs). These giant viruses exhibit unique features, including a large genome, ranging from 100 kb to 2.5 Mb and including from 150 to more than 2,500 genes, as well as the presence of genes coding for proteins involved in transcription and translation. The large majority of Lausannevirus open reading frames have unknown functions. Interestingly, a bifunctional dihydrofolate reductase-thymidylate synthase (DHFR-TS) is encoded in the Lausannevirus genome. The enzyme plays central roles in DNA precursor biosynthesis. DHFR is the pharmacological target of antifolates, such as trimethoprim, pyrimethamine, and proguanil. First, the functionality of Lausannevirus DHFR-TS was demonstrated by the successful complementation of a DHFR-deficient Saccharomyces cerevisiae strain with a plasmid expressing the heterologous gene. Additionally, using this heterologous expression system, we demonstrated the in vitro susceptibility of Lausannevirus DHFR-TS to proguanil and its resistance to pyrimethamine and trimethoprim. Proguanil may provide a unique and useful treatment if Lausannevirus proves to be a human pathogen. To our knowledge, this is the first time that a DHFR-TS has been described and characterized in an NCLDV.

scholarly journals Ochroborbone, A New Cytotoxic Indole Alkaloid from Ochrosia borbonica

2017 ◽  
Vol 12 (4) ◽  
pp. 1934578X1701200
Author(s):  
Yan-Ping Liu ◽  
A-Hong Chen ◽  
Ruo-Heng Li ◽  
Hui-Wen Yang ◽  
Hai-Nan Bao ◽  
...  
Keyword(s):  
Human Cancer ◽  
Indole Alkaloid ◽  
Spectroscopic Methods ◽  
Inhibitory Effects ◽  
Human Cancer Cell Lines ◽  
Stems And Leaves ◽  
Monoterpenoid Indole Alkaloid ◽  
First Time ◽  
Mcf 7

A new monoterpenoid indole alkaloid, ochroborbone (1), along with five known alkaloids (2–6), were isolated from the stems and leaves of Ochrosia borbonica. Among them, ochroborbone (1) is a rare C17-nor monoterpenoid indole alkaloid, and the known compounds (2-6) were isolated from Ochrosia for the first time. These structures were established on the basis of extensive spectroscopic methods. All isolated compounds were evaluated for their cytotoxicities against five human cancer cell lines: HL-60, SMMC-7721, A-549, MCF-7 and SW480 in vitro. Compounds 1 and 2 exhibited inhibitory effects with IC50 values comparable with those of cisplatin.

Identification of benzisoquinolinone derivatives with cytotoxicities from the leaves of Portulaca oleracea

2019 ◽  
Vol 74 (5-6) ◽  
pp. 139-144
Author(s):  
Rong-Rui Wei ◽  
Qin-Ge Ma ◽  
Guo-Yue Zhong ◽  
Ming Yang ◽  
Zhi-Pei Sang
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
A549 Cell ◽  
Positive Control ◽  
Portulaca Oleracea ◽  
Spectroscopic Methods ◽  
First Time ◽  
Mcf 7

Abstract Three new benzisoquinolinones (1–3), together with seven known benzisoquinolinone derivatives (4–10), were isolated from Portulaca oleracea for the first time. The structures of the isolated compounds (1–10) had been elucidated on the basis of extensive spectroscopic methods including ultraviolet, infrared, mass spectrometry, and nuclear magnetic resonance techniques and by comparison with data reported in the references. All isolated compounds were assayed for cytotoxicities against selected human lines in vitro by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay. Compounds 1, 2, 4, and 7 showed important cytotoxicities against HCT116, MCF-7, U87, and A549 cell lines with IC50 values in the range of 11.62–84.45 μM, which compared with positive control doxorubicin.

scholarly journals Inhibition of Lysyl Oxidases Impairs Migration and Angiogenic Properties of Tumor-Associated Pericytes

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Aline Lopes Ribeiro ◽  
Carolini Kaid ◽  
Patrícia B. G. Silva ◽  
Beatriz A. Cortez ◽  
Oswaldo Keith Okamoto
Keyword(s):  
Tumor Development ◽  
Therapeutic Targeting ◽  
Cancer Hallmarks ◽  
Lysyl Oxidases ◽  
The Family ◽  
Tumor Microenviroment ◽  
Cellular Components ◽  
First Time

Pericytes are important cellular components of the tumor microenviroment with established roles in angiogenesis and metastasis. These two cancer hallmarks are modulated by enzymes of the LOX family, but thus far, information about LOX relevance in tumor-associated pericytes is lacking. Here, we performed a comparative characterization of normal and tumoral pericytes and report for the first time the modulatory effects of LOX enzymes on activated pericyte properties. Tumoral pericytes isolated from childhood ependymoma and neuroblastoma specimens displayed angiogenic properties in vitro and expressed typical markers, including CD146, NG2, and PDGFRβ. Expression of all LOX family members could be detected in both normal and tumor-associated pericytes. In most pericyte samples, LOXL3 was the family member displaying the highest transcript levels. Inhibition of LOX/LOXL activity with the inhibitor β-aminopropionitrile (βAPN) significantly reduced migration of pericytes, while proliferation rates were kept unaltered. Formation of tube-like structures in vitro by pericytes was also significantly impaired upon inhibition of LOX/LOXL activity with βAPN, which induced more prominent effects in tumor-associated pericytes. These findings reveal a novel involvement of the LOX family of enzymes in migration and angiogenic properties of pericytes, with implications in tumor development and in therapeutic targeting tumor microenvironment constituents.

scholarly journals Manipulation of Plant Growth Regulators on Phytochemical Constituents and DNA Protection Potential of the Medicinal Plant Arnebia benthamii

2018 ◽  
Vol 2018 ◽  
pp. 1-8
Author(s):  
Javid A. Parray ◽  
Azra N. Kamili ◽  
Sumira Jan ◽  
Mohammad Yaseen Mir ◽  
Nowsheen Shameem ◽  
...  
Keyword(s):  
Oxidative Dna Damage ◽  
Chemical Constituents ◽  
Growth Hormones ◽  
The Family ◽  
Phytochemical Constituents ◽  
Protection Potential ◽  
First Time ◽  
Indole 3 Acetic Acid ◽  
Culture Protocol

Arnebia benthamii of the family Boraginaceae is a critically endangered nonendemic plant of the Kashmir Himalayas and is used to treat a number of human diseases. The current study was based on developing an in vitro micropropagation protocol vis-à-vis induction of various secondary metabolites under in vitro conditions for the possible biological activity. A tissue culture protocol was developed for A. benthamii for the first time in the Himalayan region using varied combinations and proper media formulations, including various adjuvants: Murashige and Skoog (MS) media, growth hormones, sugars, agar, and so forth. The influence of different media combinations was estimated, and the MS + thidiazuron (TDZ) + indole 3-acetic acid (IAA) combination favors a higher regeneration potential. The higher amounts of chemical constituents were also recorded on the same treatment. The in vitro plant samples also showed a noteworthy effect of scavenging of hydroxyl radicals vis-à-vis protection from oxidative DNA damage. The in vitro raised plants are good candidates for the development of antioxidant molecules.

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