scholarly journals Chemotaxonomy as a Potential Method to Rapidly Identify Various Namibian Plocamium Species

2018 ◽  
Vol 13 (12) ◽  
pp. 1934578X1801301 ◽  
Author(s):  
Michael G. Knott ◽  
Anthony Ishola
Keyword(s):  
Literature Review ◽  
Marine Algae ◽  
Potential Method ◽  
Rapid Identification ◽  
H Nmr ◽  
Ms Analysis ◽  
Minor Metabolite ◽  
Species Being

Differentiating between different Plocamium species of marine algae is not a trivial task. However, a literature review reveals that each species of Plocamium has a major and minor metabolite that is unique to a particular Plocamium species being investigated. Knowledge about the chemotaxonomy of these unique major and minor metabolite standards therefore enables the potentially rapid identification of Plocamium species by means of either 1H NMR or GC-MS analysis of crude Plocamium extracts.

scholarly journals Epidural hematoma in newborn: literature review

2020 ◽  
pp. e402020
Author(s):  
Isabela do Prado Nascimento ◽  
Gabriela Ferreira Kalkmann ◽  
Leticia Adrielle Dos Santos ◽  
Letícia Novak Crestani ◽  
Carlos Umberto Pereira
Keyword(s):  
High Risk ◽  
Literature Review ◽  
Brain Damage ◽  
Epidural Hematoma ◽  
Clinical Presentation ◽  
Rapid Identification ◽  
Intracranial Hemorrhages ◽  
Neurological Sequelae ◽  
Type Of Delivery

Introduction: Newborn represent a population at high risk for brain damage. Intracranial hemorrhages in newborn are uncommon, and epidural hematoma (EH) is rare. Material and methods: The authors present a literature review about EH in newborn. Results: EH in newborn occurs in only 2% and is frequently associated with the type of delivery. The clinical presentation is asymptomatic or heterogeneous. The diagnosis is made from anamnesis and imaging exams, and the treatment depends on the size of the EH. Conclusion: The knowledge of neurosurgeons, obstetricians and pediatricians about the occurrence of EH in newborn is extremely important. The rapid identification and management of the case is of paramount importance, to reduce neurological sequelae.

(Cyclopentadienyl)dihalogenochromium-Donor Compounds. Synthesis and NMR Investigation

1995 ◽  
Vol 50 (11) ◽  
pp. 1739-1747 ◽  
Author(s):  
Brigitte Bräunlein ◽  
Frank H. Köhler ◽  
Werner Strauß ◽  
Harald Zeh
Keyword(s):  
Dynamic Behavior ◽  
Spin Density ◽  
Nmr Spectra ◽  
Rapid Identification ◽  
Spin Transfer ◽  
H Nmr ◽  
Electronegative Substituent ◽  
Intramolecular Dynamic ◽  
Nmr Signals

A series of paramagnetic (S = 3/2) chromium half-sandwiches of the type CpCrX2D (D = donor) were synthesized by starting from [CpCrX2]2, Cp2Cr, CpCrCl2(THF), and Cp*Cr(CH3)2[P(CH3)3]. Besides the parent cyclopentadienyl (Cp) the alkylated derivatives CH3C5H4, (CH3)5C5 (Cp*), and C2H5(CH3)4C5 were bound to chromium. The electronegative substituent was X = F, Cl, Br, I, and triflate, while the donors were three ethers, four ketones, dimethylsulfoxide, acetonitrile, methylisocyanide, pyridine, and seven molecules ER3 where E = N, P, As, Sb. The half-sandwiches were partly isolated and partly established in solution. The 13C and 1H NMR spectra showed strongly shifted signals which allowed to quantitatively investigate the equilibrium between CpCrX2D and the anti ferromagnetic species [CpCrX2]2. The NMR signals of CpCrX2D and its substituted derivatives appeared in characteristic ranges thus providing a means of rapid identification. Considerable spin density was found to be induced on the ligands; it is negative in the Cp π system. As for the donors, inter- and intramolecular dynamic behavior as well as selective spin transfer to the γ protons of acetonitrile, methylisocyanide, and ketones was detected.

scholarly journals Atopobium vaginae: a literature review, on findings and potential clinical implications

2019 ◽  
Vol 34 (1) ◽  
Author(s):  
Andrea Mariano ◽  
Angelo Salzo ◽  
Valentina Felice ◽  
Massimiliano Scutellà
Keyword(s):  
Literature Review ◽  
Inflammatory Disease ◽  
Technological Progress ◽  
State Of The Art ◽  
Rapid Identification ◽  
Clinical Implications ◽  
Positive Bacterium ◽  
Clinical Scenarios ◽  
Invasive Infections ◽  
Atopobium Vaginae

Atopobium vaginaeis an anaerobic Gram-positive bacterium. It is recognized as a causal agent of bacterial vaginosis onset and occasionally associated to gynaecologic-obstetric complications (including pelvic inflammatory disease, endometritis, tubo-ovarian abscesses and preterm delivery with neonatal consequences). Additionally, bacteraemia and invasive infections have rarely been reported. The scientific and technological progress allowed an accurate and rapid identification of Atopobium vaginae supporting diagnosis and clinic interventions. In this article, literature has been deeply examined to report the state-of-the-art on Atopobium vaginae, which might be useful in clinical scenarios.

scholarly journals Rapid identification of bacterial isolates from wheat roots by high resolution whole cell MALDI-TOF MS analysis

2013 ◽  
Vol 165 (3-4) ◽  
pp. 167-174 ◽  
Author(s):  
Maria Isabel Stets ◽  
Artur Soares Pinto ◽  
Luciano Fernandes Huergo ◽  
Emanuel Maltempi de Souza ◽  
Vandeir Francisco Guimarães ◽  
...  
Keyword(s):  
High Resolution ◽  
Rapid Identification ◽  
Bacterial Isolates ◽  
Wheat Roots ◽  
Whole Cell ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Tof Ms

scholarly journals Benthic marine algae of the coral reefs of Brazil: a literature review

2008 ◽  
Vol 12 (02) ◽  
pp. 258-269 ◽  
Author(s):  
Marcia Abreu de Oliveira Figueiredo ◽  
Paulo Antunes Horta ◽  
Alexandre de Gusmão Pedrini ◽  
José Marcos de Castro Nunes
Keyword(s):  
Literature Review ◽  
Coral Reefs ◽  
Marine Algae

Ultrastructural characteristics of the non-immune rosette-forming cell

1975 ◽  
Vol 18 (1) ◽  
pp. 79-96
Author(s):  
J.D. Levy ◽  
M.R. Knieser ◽  
W.A. Briggs
Keyword(s):  
T Cells ◽  
T Lymphocyte ◽  
Cell Activation ◽  
Potential Method ◽  
Rapid Identification ◽  
Red Cell Membrane ◽  
Culture Techniques ◽  
In Vitro Technique ◽  
Cell Fragments

Non-immune rosette formation, where a non-sensitized lymphocyte is surrounded by three or more sheep red blood cells, is an in vitro technique which is thought to be specific in the identification of thymus-dependent (T) lymphocytes. Using the electron microscope and tissue culture techniques, we have studied the ultrastructure of the rosette-forming lymphocyte which has been stimulated by various cellular mitogens. Non-sensitized but stimulated lymphocytes, which form rosettes, have a morphology similar to that of previously identified T cells, adding further credence to the concept that non-sensitized rosette-forming cells represent a population of T cells. Changes in the binding pattern of the sheep red cell membrane to the lymphocyte have been identified which may represent an early phase of cell ‘activation’. This marker of activation offers a potential method of studying membrane responses in attempts to localize cellular defects involving the T lymphocyte. In addition, preliminary studies using fluorescein-labelled sheep red blood cell fragments suggest specific attachment of labelled membrane to the T lymphocyte, allowing rapid identification of T cells in smears or tissue preparations.

scholarly journals Cinnamoylphenethylamine 1H-NMR Chemical Shifts: A Concise Reference for Ubiquitous Compounds

2010 ◽  
Vol 5 (8) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Hans A. Pedersen ◽  
Stine K. Steffensen ◽  
Carsten Christophersen
Keyword(s):  
Natural Products ◽  
1H Nmr ◽  
Spectroscopic Data ◽  
Uv Light ◽  
Chemical Shifts ◽  
Rapid Identification ◽  
Upfield Shift ◽  
Nmr Chemical Shifts ◽  
H Nmr ◽  
Nmr Data

1H-NMR data of 25 cinnamoylphenethylamine derivates were recorded and compared in order to assign signals unequivocally without additional spectroscopic data. The spectra provide a key for the rapid identification of these ubiquitous natural products. The compounds isomerize rapidly in UV light, producing a characteristic upfield shift of the olefinic protons consistent with distorted planarity of the Cis cinnamate, and this requires special attention during preparative work.

Comparative RP-HPLC for rapid identification of glycopeptides and application in off-line LC-MALDI-MS analysis

2008 ◽  
Vol 343 (4) ◽  
pp. 758-768 ◽  
Author(s):  
Yoshimi Kanie ◽  
Akiko Enomoto ◽  
Satoshi Goto ◽  
Osamu Kanie
Keyword(s):  
Rapid Identification ◽  
Maldi Ms ◽  
Rp Hplc ◽  
Ms Analysis

scholarly journals Metabolic profiling of Candida clinical isolates of different species and infection sources

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Josidel Conceição Oliver ◽  
Luca Laghi ◽  
Carola Parolin ◽  
Claudio Foschi ◽  
Antonella Marangoni ◽  
...  
Keyword(s):  
Fungal Infections ◽  
Tricarboxylic Acid Cycle ◽  
Acid Synthesis ◽  
Rapid Identification ◽  
Clinical Isolates ◽  
H Nmr ◽  
Source Of Infection ◽  
Sources Of Infection ◽  
Secreted Aspartyl Proteinase ◽  
Different Sources

Abstract Candida species are the most common cause of opportunistic fungal infections. Rapid identification and novel approaches for the characterization of these fungi are of great interest to improve the diagnosis and the knowledge about their pathogenic properties. This study aimed to characterize clinical isolates of Candida spp. by proteomics (MALDI-TOF MS) and metabolomics (1H-NMR), and to correlate their metabolic profiles with Candida species, source of infection and different virulence associated parameters. In particular, 49 Candida strains from different sources (blood, n = 15; vagina, n = 18; respiratory tract, n = 16), belonging mainly to C. albicans complex (61%), C. glabrata (20%) and C. parapsilosis (12%) species were used. Several extracellular and intracellular metabolites showed significantly different concentrations among isolates recovered from different sources of infection, as well as among different Candida species. These metabolites were mainly related to the glycolysis or gluconeogenesis, tricarboxylic acid cycle, nucleic acid synthesis and amino acid and lipid metabolism. Moreover, we found specific metabolic fingerprints associated with the ability to form biofilm, the antifungal resistance (i.e. caspofungin and fluconazole) and the production of secreted aspartyl proteinase. In conclusion, 1H-NMR-based metabolomics can be useful to deepen Candida spp. virulence and pathogenicity properties.

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