scholarly journals Improvement Effect of Ficus vasculosa Ethanol Extract on D-galactose-Induced Mice Aging

2019 ◽  
Vol 14 (12) ◽  
pp. 1934578X1989667
Author(s):  
Jing-Jing Li ◽  
Ling Mo ◽  
Jia-Le Song
Keyword(s):  
Antioxidant Activity ◽  
Enzyme Activity ◽  
Reducing Power ◽  
Ethanol Extract ◽  
Trienoic Acid ◽  
Radical Scavenge Activity ◽  
Hepatic Tissue ◽  
Total Serum

This study was to investigate antioxidant activities of the ethanol extract from young edible leaves of Ficus vasculosa in vitro and in vivo . Ficus vasculosa ethanol extract (FVEE) showed significantly higher reducing power and α,α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenge activity than vitamin C ( P < 0.05). FVEE also showed an activity to resist the D-galactose-induced aging in mice assessed by serum and tissue levels of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px). Total serum and tissue oxidative status, total antioxidantresponse, glutathione (GSH) and malondialdehyde (MDA) levels have been also measured. Pretreatment with FVEE at 200 mg/kg·body weight significantly increased enzyme activity of SOD and CAT in serum and hepatic tissue ( P < 0.05), as well as significantly increased enzyme activity of SOD in kidney ( P < 0.05). Furthermore, high concentration of FVEE pretreatment significantly increased the level of GSH in serum, hepatic tissue and kidney ( P < 0.05), meanwhile significantly decreased MDA production in hepatic tissue and kidney ( P < 0.05). In addition, the phytochemical investigation discovered six previously described compounds from FVEE, naringenin (1), vanillic acid (2), 9, 16-dioxo-10, 12, 14-octadeca-trienoic acid (3), 2, 6-dimethoxy-1, 4-benzoquinone (4), apigenin (5) and norartocarpetin (6), and all compounds were isolated from this plant for the first time. Among the various compounds found, the rare highly unsaturated fatty acid 9, 16-dioxo-10, 12, 14-octadeca-trienoic acid (3) has been identified, which had been isolated only once before from F. vasculosa. Evaluation of the antioxidant activity of isolated compounds showed naringenin (1) to be the most active. According to our research, FVEE present very high antioxidant activity in vitro due to the presence of several compounds known for their antioxidant activity such as flavonoid and phenolic acid. In vivo, the ethanol extract had improvement effects against D-galactose-induced aging by reducing oxidative stress.

Antioxidant Activity, Phenolic Content and Hematoprotective Effects of Cleome arabica Polyphenolic Leaf Extract

2019 ◽  
Author(s):  
C. Tigrine ◽  
A. Kameli
Keyword(s):  
Antioxidant Activity ◽  
Biological Effects ◽  
Reducing Power ◽  
Hematological Toxicity ◽  
Protective Effects ◽  
Ferrous Ions ◽  
Polyphenolic Extract ◽  
Cleome Arabica

In this study a polyphenolic extract from Cleome arabica leaves (CALE) was investigated for its antioxidant activity in vitro using DPPH•, metal chelating and reducing power methods and for its protective effects against AraC-induced hematological toxicity in vivo using Balb C mice. Results indicated that CALE exhibited a strong and dose-dependent scavenging activity against the DPPH• free radical (IC50 = 4.88 μg/ml) and a high reducing power activity (EC50 = 4.85 μg/ml). Furthermore, it showed a good chelating effects against ferrous ions (IC50 = 377.75 μg/ml). The analysis of blood showed that subcutaneous injection of AraC (50 mg/kg) to mice during three consecutive days caused a significant myelosupression (P < 0.05). The combination of CALE and AraC protected blood cells from a veritable toxicity. Where, the number of the red cells, the amount of hemoglobin and the percentage of the hematocrite were significantly high. On the other hand, AraC cause an elevation of body temperature (39 °C) in mice. However, the temperature of the group treated with CALE and AraC remained normal and did not exceed 37.5 °C. The observed biological effects of CALE, in vitro as well as in vivo, could be due to the high polyphenol and flavonoid contents. In addition, the antioxidant activity of CALE suggested to be responsible for its hematoprotective effect.

scholarly journals In Vitro and In Vivo Antioxidant Activity of Agave sisalana Agro-Industrial Residue

Biomolecules ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1435 ◽  
Author(s):  
Stella Maria Andrade Gomes Barreto ◽  
Cesar Orlando Muñoz Cadavid ◽  
Rafael Amir de Oliveira Moura ◽  
Giovanna Melo Martins Silva ◽  
Samara Vitória Ferreira de Araújo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Direct Action ◽  
Radical Scavenging ◽  
Reducing Power ◽  
New Production ◽  
Industrial Residue ◽  
Agave Sisalana

Agave sisalana agro-industrial residue has considerable potential against damage associated with oxidative stress and skin aging. This study aims to demonstrate, in vitro and in vivo, the potential of Agave sisalana agro-industrial residue as a safe and effective alternative for the prevention of damage caused by oxidative stress and aging. The antioxidant activity was evaluated in vitro (total antioxidant capacity, reducing power, DPPH radical scavenging, metal chelating (Fe2+ and Cu2+), and hydroxyl radical scavenging) and in vivo using the Caenorhabditis elegans organism model. The extract showed in vitro antioxidant activity in all tests performed. Tests with C. elegans showed that the extract was able to reduce the intracellular levels of reactive oxygen species (ROS) and increase the survival rate of worms. A downregulation of gst-4::GFP expression suggests a direct action against free radicals. Agave sisalana agro-industrial residue extract (AsRE) can therefore be considered as a source of antioxidant biomolecules, and the use of this agro-industrial residue in a new production process can lead to sustainability and socioeconomic development.

scholarly journals IN VITRO ANTIOXIDANT AND IN VIVO ANTI-INFLAMMATORY ACTIVITY OF THE AERIAL PART OF BLUMEA ERIANTHA DC

2018 ◽  
Vol 10 (7) ◽  
pp. 75 ◽  
Author(s):  
Urmila U. Tambewagh ◽  
Supada Rambhau Rojatkar
Keyword(s):  
Antioxidant Activity ◽  
Aerial Part ◽  
Methanol Extract ◽  
Reducing Power ◽  
Dependent Manner ◽  
Paw Edema ◽  
Anti Inflammatory ◽  
In Vitro Antioxidant Activity

Objective: Objective of the present study was to carry out in vivo anti-inflammatory and in vitro antioxidant activity of methanol extract of aerial part of the Blumea eriantha DC belonging to family Asteraceae.Methods: The shade dried aerial part of B. eriantha (0.5 kg) was powdered and extracted with methanol (1.5 x 3L) at room temperature (24h x 3). After filtration combined all the three extracts and were concentrated on rotary evaporator under reduced pressure at 40 °C, thereby providing crude methanol extract which was subsequently employed for further studies. Anti-inflammatory effect was studied by carrageenan-induced paw edema model in rats at dose level 100, 200, and 400 mg/kg. Acute oral toxicity study and in vitro antioxidant potential of the extract was also studied. The in vitro antioxidant activity of methanol extract of aerial part of Blumea eriantha DC was evaluated against 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide (H2O2) and hydroxyl (OH) radicalscavenging and reducing power assays.Results: The results indicate that methanol extract of Blumea eriantha (BEME, 400 mg/kg) exhibited significant inhibition (p<0.001) of increase in paw edema at 5th h. IC50 value of BEME showed significant antioxidant activity. The extract exhibits promising free radical scavenging effect of DPPH, H2O2, OH and reducing power in a dose-dependent manner up to 100µg/ml concentration while the reference standard Ascorbic acid demonstrated more scavenging potential than the methanol extract of Blumea eriantha The methanol extract was found to be safe at the dose of 2000 mg/kg.Conclusion: The results of the experimental study confirmed that methanol extract of Blumea eriantha DC possesses significant anti-inflammatory and antioxidant activity.

scholarly journals Optimization of Phenolic Compound Extraction from Chinese Moringa oleifera Leaves and Antioxidant Activities

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Beibei Zhao ◽  
Jiawen Deng ◽  
Hua Li ◽  
Yaqiang He ◽  
Tao Lan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Moringa Oleifera ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ultrasonic Extraction ◽  
Extraction Temperature ◽  
Moringa Oleifera Leaves

Rich in phenolic compounds, Moringa oleifera leaf extract (ME) exhibits significant antioxidant activity both in vitro and in vivo. ME has already been widely used in fields of medicine, functional food, and cosmetics. Ultrasonic extraction (UE) method has been improved to be one of the most effective ways to extract phenols from M. oleifera leaves. The purpose of this study was to optimize ultrasonic extraction of phenols by response surface methodology (RSM). Four parameters were discussed, such as ethanol concentration, solvent-sample ratio, extraction temperature, and extraction time. Also, purification methods of the crude ME by organic solvent extraction and column chromatography were examined. Antioxidant activities of ME and each fraction were evaluated by DPPH, ABTS, and hydroxy radical-scavenging activities and reducing power. The phenol content of the purified ME reached up to 962.6 mg RE/g, extremely higher than the crude extract 107.22 ± 1.93 mg RE/g. The antioxidant activity of the purified ME was also significantly improved. Furthermore, phenols were identified by using the HPLC-MS method, and the results showed that there were 6 phenolic acids and derivatives and 7 flavonoids in ME. Quercetin-3-O-β-D-glucoside isolated from ME showed excellent DPPH and ABTS radical-scavenging abilities, which were comparable to VC.

scholarly journals Study of Antioxidant Activity of Stalk and Fruit of Solanum melongena L. (Solanaceae)

2019 ◽  
pp. 1-7
Author(s):  
Kady Diatta ◽  
William Diatta ◽  
Alioune Dior Fall ◽  
Serigne Ibra Mbacké Dieng ◽  
Amadou Ibrahima Mbaye ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Solanum Melongena ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Natural Antioxidants ◽  
In Vivo Studies ◽  
Ethanol Extracts

Background : Nowadays with the appearance of diseases such as cancer, atherosclerosis, free radicals are often singled out. What motivates scientific research in natural antioxidants. Aim/Objective : The aim of this study was to determine the antioxidant activity of the stalks and the fruit of Solanum melongena L. Study Duration : The period of the study was done on 25th July, 2015 at the Department of Pharmacy, Faculty of Medecine, Pharmacy and Odontology, University of Dakar, Senegal. Methodology : Antioxidant activity was evaluated through two methods (DPPH and FRAP). Results : For the FRAP test, at the highest concentration (83.3 µg/ml) the aqueous extract of the fruit (0.90±0.08) has a higher reducing power compared to those of ethanol extracts from the fruit (0.77±0.41) and the stalk (0.85±0.004). These results remain inferior to that of tannic acid (0.95± 0.0005). The DPPH test reveals that the ethanolic extract of the fruit is more effective in reducing the free radical DPPH with an inhibitory concentration 50 (IC 50) equal to 3.37±0.03 μg / ml, followed by the ethanolic extract of the stalks (IC 50 = 4.46±0.24 μg / ml) and finally the aqueous extract of the fruit (IC50 = 9.6±0.026 μg / ml). Conclusion : These results make it possible to confirm the in vitro activity of the parts studied, but in vivo studies are necessary in order to know the acute and chronic toxicities. Finally, perform a bio-guided fractionation to determine the molecules responsible for the antioxidant activity.

scholarly journals IN VITRO AND IN VIVO STUDIES OF ANTIHYPERURICEMIC AND ANTIOXIDANT ACTIVITY FROM BULBS OF BAWANG TIWAI (ELEUTHERINE PALMIFOLIA (L.) MERR.) FROM INDONESIA

2019 ◽  
Vol 12 (1) ◽  
pp. 497
Author(s):  
Dian Ratih Laksmitawati ◽  
Rininta Firdaus ◽  
Mediana Astika Zein
Keyword(s):  
Antioxidant Activity ◽  
Body Weight ◽  
Uric Acid ◽  
Ethanol Extract ◽  
Positive Control ◽  
In Vivo Studies ◽  
Inhibit Lipid Peroxidation ◽  
Plasma Malondialdehyde

Objectives: This study would like to investigate the in vitro antioxidant activity through 2,2-diphenyl-1-picrylhydrazyl assay and in vitro xanthine oxidase activity of the bulbs. This study performs in vivo assays to study the antihyperuricemic activity and antioxidant in the hyperuricemic rat through plasma malondialdehyde measurement. Method: The study was conducted by testing the fresh bulbs of bawang tiwai (Eleutherine palmifolia (L.) Merr. with chemical solvent of ethanol 70% to extract the bulbs. Allopurinol and Vitamin C were used as positive control for the antihyperuricemic assay and antioxidant assay, respectively. Other chemical substances were also used in this study. This study used chicken extract (Brands) 20 ml/kg/body weight to induce the level of uric acid in the blood serum, and potassium oxonate (Sigma 156124) to inhibit the uricase in rats. Results: The results show that the levels of uric acid were measured using spectrophotometer with dichloro-hydroxybenzen sulfonate (Biolabo) a as reagent. The ethanol extract of bawang tiwai (EBT) (E. palmifolia (L.) Merr) was potential to reduce uric acid level at 140, 280, and 560 mg/kg body weight, but possibly without inhibition against xanthine oxydase activity. Conclusion: All doses of EBT could inhibit lipid peroxidation in hyperuricemic condition caused by high purine diet in 14 days.

scholarly journals COMPARATIVE STUDIES OF PHYTOCHEMICAL AND ANTIOXIDANT ACTIVITY OF IN VIVO PLANT AND IN VITRO CALLUS EXTRACT OF CARDIOSPERMUM HALICACABUM L.

2021 ◽  
pp. 94-103
Author(s):  
THANIARASU R ◽  
LOGESHWARI M
Keyword(s):  
Antioxidant Activity ◽  
Biological Activities ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Dry Weight ◽  
Biologically Active ◽  
Diffusion Method ◽  
Total Phenolic

Objective: The present investigation focuses on the use of Cardiospermum halicacabum L. in their phytochemical and biological activities. Methods: In this study, in vivo stem and in vitro callus ethanolic extracts of C. halicacabum were tested for their phytochemical attributes by qualitative method, Fourier transform infrared (FTIR), antioxidant, antibacterial, and bioactive compound properties. The bactericidal activity of the in vivo stem and in vitro callus extract has been evaluated in both Gram+ve and Gram-ve microorganisms using the disk diffusion method. Results: The highest frequency (78%) of well developed, dark green organogenic callus was induced from stem explant on Murashige and Skoog (MS) medium supplemented with 0.7 mg/l 2,4-Dichlorophenoxyacetic acid (2, 4-D) and 0.5 mg/l benzyl adenine (BA). The results of FTIR spectra confirmed the presence of functional groups in wild stem and in vitro callus extract of C. halicacabum with various peaks. The total phenolic content in ethanolic extract of in vivo plant and in vitro callus was 80.46 mg gallic acid equivalent (GAE)/g dry weight and 76.4 mg GAE/g dry weight, respectively. The highest percentage of tannins was measured at 78.03 in wild stem ethanol extracts followed by 75.22 in callus extract. The antioxidant activity of 2,2-diphenyl-2- picrylhydrazyl (DPPH) ethanol extract was found to be 206.54 μg/ml. IC50 values of the stem extracts of C. halicacabum are 306 μg/ml and 286 μg/ml in callus extract, respectively. Antibacterial activity of the ethanol extract was higher for Staphylococcus aureus (S. aureus) with a 17 mm zone of inhibition. Conclusion: The present investigation recommended that the callus ethanolic extract function as a good source of biologically active compounds and natural antioxidants.

scholarly journals The STANDARDIZATION OF A TRADITIONAL POLYHERBAL FORMULATION WITH PHARMACOGNOSTIC STUDY; ITS PHYTOCHEMICAL CONTENT, ANTIOXIDANT, AND ANTIDIABETIC ACTIVITY

2019 ◽  
pp. 182-190
Author(s):  
ARUNIKA SUBBA ◽  
PALASH MANDAL ◽  
Arunika Subba
Keyword(s):  
Antioxidant Activity ◽  
Reducing Power ◽  
Acetone Extract ◽  
In Vivo Studies ◽  
Antidiabetic Activity ◽  
Scavenging Activity ◽  
Phytochemical Content ◽  
Soxhlet Apparatus

Objective: Pharmacognostic study, evaluation of antioxidant and antidiabetic activity along with phytochemical contents of an ethnomedicine (AR) which is used for the treatment of arthritis and diabetes in some villages of West Sikkim. Methods: The herbal formulation was extracted in a Soxhlet apparatus successively in ten solvents from low to high polarity. The extracts were subjected to antioxidant activity, qualitative and quantitative phytochemical estimation as well as in vitro antidiabetic activity. For pharmacognostic characterization, parameters such as fluorescence activity, physicochemical values, powder microscopy, and thin-layer chromatography (TLC) were performed. Mean values with p<0.05 were considered significant in statistical analysis. Results: Pharmacognostic study revealed various plants tissues. Ash values suggested the presence of earthy materials and moisture content near to the maximum range. Variation of colors was exhibited by AR in fluorescence analysis. TLC expressed the presence of phytoconstituents and the Rf values were noted down to be used in the future for authentication of the sample. Potential antioxidant capacity was observed in AR, phenolics significantly contributing in 1,1-diphenyl-2-picrylhydrazyl scavenging activity, 2, 2’-azinobis (3-ethylbenzothiazoline-6-sulfonic acid)+ scavenging activity and reducing power. Non-polar solvent showed the presence of alkaloid and steroids. The antidiabetic activity was very high in some extracts of AR with acetone extract showing the highest enzyme inhibiting activity. IC50 of acetone extract was 0.26±0.003 mg/ml. Conclusion: Overall study established a basic reference for the formulation AR. It was considered to possess antioxidant activity, but the interesting part of the study was its antidiabetic activity which is needed to be validated with in vivo studies and toxicity assessment.

scholarly journals The effect of cold atmospheric plasma on diabetes-induced enzyme glycation, oxidative stress, and inflammation; in vitro and in vivo

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Alireza Rezaeinezhad ◽  
Pegah Eslami ◽  
Hossein Mirmiranpour ◽  
Hamid Ghomi
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Enzyme Activity ◽  
Fluorescence Analysis ◽  
Atmospheric Plasma ◽  
Oxidative Stress Biomarkers ◽  
Cold Atmospheric Plasma ◽  
Stress Biomarkers

AbstractCold atmospheric plasma (CAP) is known as the versatile tool in different biological, and medical applications. In this study, we investigated the effect of cold plasma on diabetes via in vitro and in vivo assessments. We performed the in vitro assay to evaluate the impact of CAP on glycated glutathione peroxidase (GPx) through enzyme activity measurement as a function index and far- and near-UV circular dichroism (CD) and fluorescence analysis as structure indices. The result of in vitro assessment showed that the exposure of glycated GPx to plasma causes a considerable increase in enzyme activity up to 30%. Also, the evaluation of far- and near-UV CD and fluorescence analysis indicated a modification in the protein structure. According to obtained result from in vitro assessment, in vivo assay evaluated the effect of CAP on diabetic mice through analyzing of blood glucose level (BGL), advanced glycation end products (AGEs), antioxidant activity, oxidative stress biomarkers such as malondialdehyde (MDA), advanced oxidation protein products (AOPP), and oxidized low-density lipoprotein (oxLDL), and inflammation factors including tumor necrosis factor (TNF-α), interleukin-1 (IL-1), and interleukin-6 (IL-6). The result of in vivo experiment also showed a 20% increase in antioxidant activity. Also, the reduction in AGEs, oxidative stress biomarkers, and inflammatory cytokines concentrations was observed. The result of this study revealed that CAP could be useful in diabetes treatment and can be utilized as a complementary method for diabetes therapy.

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