scholarly journals Tanshinone IIA reduces SW837 colorectal cancer cell viability via the promotion of mitochondrial fission by activating JNK-Mff signaling pathways

2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Sayilaxi Jieensinue ◽  
Hong Zhu ◽  
Guangcheng Li ◽  
Keli Dong ◽  
Meiting Liang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Viability ◽  
Signaling Pathways ◽  
Cancer Cell ◽  
Mitochondrial Fission ◽  
Tanshinone Iia ◽  
Colorectal Cancer Cell

Long Non-Coding RNA-Colon Cancer Associated Transcript 1 Down-Regulation Inhibits Cell Proliferation and Promotes Apoptosis in Colorectal Cancer Through Enhancing miR-152 Expression

2020 ◽  
Vol 10 (12) ◽  
pp. 1766-1772
Author(s):  
Jindong Li ◽  
Xi Wang ◽  
Xin Huang ◽  
Na Li ◽  
Ya Ling ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Viability ◽  
Cancer Cell ◽  
Cancer Progression ◽  
Biological Effects ◽  
Colorectal Cancer Cell ◽  
Luciferase Reporter ◽  
Pcr Analysis ◽  
Cancer Tissue ◽  
Colorectal Cancer Progression

Colorectal cancer is a common malignant cancer that is characterized by high mortality rate. CCAT1 is a type of newly discovered lncRNA. This research was conducted to study the role of CCAT1 in colorectal cancer. The findings showed that there was significant up-regulation of CCAT1 expression in colorectal cancer. Then, online bioinformatic database and dual-luciferase reporter assay to prove CCAT1 and miR-152 have direct binding sites. Many researches demonstrated that miR-152 played a crucial role in development of colorectal cancer. Therefore, we then explored the relationship between CCAT1 and miR-152 in colorectal cancer. qRT-PCR analysis showed that miR-152 was lowly expressed in cancer tissue and cells. We then explored the effect of CCAT1 and miR-152 on the biological effects of colorectal cancer cells. MiR-152 up-regulation significantly reduced colorectal cancer cell viability and enhanced apoptosis. Furthermore, CCAT1-shRNA inhibited colorectal cancer cell viability and enhanced cell apoptosis were significantly eliminated by miR-152 inhibitor. Combined with all results, CCAT1/miR-152 axis was related to colorectal cancer progression regulation, which might be used as new therapeutic targets for colorectal cancer treatment.

scholarly journals MiR-802 Suppresses Colorectal Cancer Cell Viability, Migration and Invasion by Targeting RAN

2020 ◽  
Vol Volume 12 ◽  
pp. 2291-2300 ◽  
Author(s):  
Hailong Feng ◽  
Lingling Liu ◽  
Laijing Xu ◽  
Haili Wang ◽  
Qiuju Hua ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Viability ◽  
Cancer Cell ◽  
Colorectal Cancer Cell ◽  
Migration And Invasion

scholarly journals The Effect of Pomegranate Juice Extract on the Viability and Morphology of Human Colorectal Cancer Cell, HCT-116

2019 ◽  
pp. 57
Author(s):  
Elsa Haniffah Mejia Mohamed ◽  
Fatin Nursyamimi Abd Razak ◽  
Kim Jun Cheng ◽  
Zaridatul Aini Ibrahim
Keyword(s):  
Colorectal Cancer ◽  
Cell Viability ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Mtt Assay ◽  
Cytotoxic Effect ◽  
Morphological Changes ◽  
Pomegranate Juice ◽  
Colorectal Cancer Cell ◽  
Hct 116

Introduction: Colorectal cancer (CRC) is the second most common cancer in Malaysia. Current available treatments used have limitations due to the occurrence of drug resistance and unfavourable side effects.Objectives: This study aims to investigate the potential cytotoxicity of pomegranate juice extract (PJE) on colorectal cancer cell line, HCT-116 colorectal cancer and CCD-841 normal colorectal cells.Methods: Half maximal inhibitory concentration of the extract (IC50) was determined and selected for further investigations. Cell viability was assessed using MTT assay and morphological changes were observed using AO/PI staining.Results: PJE induced modest cytotoxic effect against HCT-116 cancer cells. High concentrations and longer stimulation periods were needed to demonstrate the cytotoxic effect of the PJE (IC50 of 987.76 ± 210.15 μg/mL at 72-hours). AO/PI staining demonstrated that the PJE (1000 μg/mL) induced obvious morphological changes in HCT-116 cancer cells after 72 hours of treatment. Characteristics of apoptosis were observed in the cancer cells. MTT assay results showed no significant reduction in normal cell viability in every time point of treatment.Conclusion: PJE induced specific cytotoxic effect and morphological changes in HCT-116 cancer cells as compared to CCD-841 normal cells. Further studies should be conducted to determine the detailed mechanisms by which PJE induced apoptosis in HCT-116 colorectal cancer cells to uncover its potential application as an adjunct therapy against colorectal cancer.International Journal of Human and Health Sciences Supplementary Issue: 2019 Page: 57

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