scholarly journals Maize brachytic2 (br2) suppresses the elongation of lower internodes for excessive auxin accumulation in the intercalary meristem region

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Xiangge Zhang ◽  
Xianbin Hou ◽  
Yinghong Liu ◽  
Lanjie Zheng ◽  
Qiang Yi ◽  
...  
Keyword(s):  
Crop Production ◽  
Vascular Bundles ◽  
Internode Elongation ◽  
Leaf Stage ◽  
Intercalary Meristem ◽  
Auxin Concentration ◽  
Short Internodes ◽  
Underlying Mechanisms ◽  
Auxin Accumulation

Abstract Background Short internodes contribute to plant dwarfism, which is exceedingly beneficial for crop production. However, the underlying mechanisms of internode elongation are complicated and have been not fully understood. Results Here, we report a maize dwarf mutant, dwarf2014 (d2014), which displays shortened lower internodes. Map-based cloning revealed that the d2014 gene is a novel br2 allele with a splicing variation, resulting in a higher expression of BR2-T02 instead of normal BR2-T01. Then, we found that the internode elongation in d2014/br2 exhibited a pattern of inhibition-normality-inhibition (transient for the ear-internode), correspondingly, at the 6-leaf, 12-leaf and 14-leaf stages. Indeed, BR2 encodes a P-glycoprotein1 (PGP1) protein that functions in auxin efflux, and our in situ hybridization assay showed that BR2 was mainly expressed in vascular bundles of the node and internode. Furthermore, significantly higher auxin concentration was detected in the stem apex of d2014 at the 6-leaf stage and strictly in the node region for the ear-internode at the 14-leaf stage. In such context, we propose that BR2/PGP1 transports auxin from node to internode through the vascular bundles, and excessive auxin accumulation in the node (immediately next to the intercalary meristem) region suppresses internode elongation of d2014. Conclusions These findings suggest that low auxin levels mediated by BR2/PGP1 in the intercalary meristem region are crucial for internode elongation.

RyR1 exhibits lower gain of CICR activity than RyR3 in the SR: evidence for selective stabilization of RyR1 channel

2004 ◽  
Vol 287 (1) ◽  
pp. C36-C45 ◽  
Author(s):  
Takashi Murayama ◽  
Yasuo Ogawa
Keyword(s):  
Binding Sites ◽  
Skeletal Muscles ◽  
Specific Activity ◽  
Adenine Nucleotides ◽  
Minor Effect ◽  
Mammalian Skeletal Muscle ◽  
Selective Stabilization ◽  
Underlying Mechanisms ◽  
A Minor

We showed that frog α-ryanodine receptor (α-RyR) had a lower gain of Ca2+-induced Ca2+ release (CICR) activity than β-RyR in sarcoplasmic reticulum (SR) vesicles, indicating selective “stabilization” of the former isoform (Murayama T and Ogawa Y. J Biol Chem 276: 2953–2960, 2001). To know whether this is also the case with mammalian RyR1, we determined [3H]ryanodine binding of RyR1 and RyR3 in bovine diaphragm SR vesicles. The value of [3H]ryanodine binding (B) was normalized by the number of maximal binding sites (Bmax), whereby the specific activity of each isoform was expressed. This B/Bmax expression demonstrated that ryanodine binding of individual channels for RyR1 was <15% that for RyR3. Responses to Ca2+, Mg2+, adenine nucleotides, and caffeine were not substantially different between in situ and purified isoforms. These results suggest that the gain of CICR activity of RyR1 is markedly lower than that of RyR3 in mammalian skeletal muscle, indicating selective stabilization of RyR1 as is true of frog α-RyR. The stabilization was partly eliminated by FK506 and partly by solubilization of the vesicles with CHAPS, each of which was additive to the other. In contrast, high salt, which greatly enhances [3H]ryanodine binding, caused only a minor effect on the stabilization of RyR1. None of the T-tubule components, coexisting RyR3, or calmodulin was the cause. The CHAPS-sensitive intra- and intermolecular interactions that are common between mammalian and frog skeletal muscles and the isoform-specific inhibition by FKBP12, which is characteristic of mammals, are likely to be the underlying mechanisms.

scholarly journals Mitochondria Endoplasmic Reticulum Contact Sites (MERCs): Proximity Ligation Assay as a Tool to Study Organelle Interaction

2021 ◽  
Vol 9 ◽  
Author(s):  
Sara Benhammouda ◽  
Anjali Vishwakarma ◽  
Priya Gatti ◽  
Marc Germain
Keyword(s):  
Endoplasmic Reticulum ◽  
Fluorescence Probes ◽  
Proximity Ligation Assay ◽  
Proximity Ligation ◽  
Membrane Contact Sites ◽  
Contact Sites ◽  
Membrane Contact ◽  
Endogenous Proteins ◽  
Underlying Mechanisms

Organelles cooperate with each other to regulate vital cellular homoeostatic functions. This occurs through the formation of close connections through membrane contact sites. Mitochondria-Endoplasmic-Reticulum (ER) contact sites (MERCS) are one of such contact sites that regulate numerous biological processes by controlling calcium and metabolic homeostasis. However, the extent to which contact sites shape cellular biology and the underlying mechanisms remain to be fully elucidated. A number of biochemical and imaging approaches have been established to address these questions, resulting in the identification of a number of molecular tethers between mitochondria and the ER. Among these techniques, fluorescence-based imaging is widely used, including analysing signal overlap between two organelles and more selective techniques such as in-situ proximity ligation assay (PLA). While these two techniques allow the detection of endogenous proteins, preventing some problems associated with techniques relying on overexpression (FRET, split fluorescence probes), they come with their own issues. In addition, proper image analysis is required to minimise potential artefacts associated with these methods. In this review, we discuss the protocols and outline the limitations of fluorescence-based approaches used to assess MERCs using endogenous proteins.

Preliminary Experimental Study of Warm Ultrasonic Impact-assisted Laser Metal Deposition

2021 ◽  
pp. 1-15
Author(s):  
Hanyu Song ◽  
Minglang Li ◽  
Muxuan Wang ◽  
Benxin Wu ◽  
Ze Liu ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Experimental Study ◽  
Solid Material ◽  
Metal Deposition ◽  
Laser Metal Deposition ◽  
Ultrasonic Impact Treatment ◽  
Post Process ◽  
Underlying Mechanisms ◽  
Manufacturing Time

Abstract A preliminary experimental study on “warm ultrasonic impact-assisted laser metal deposition” (WUI-LMD) is reported, and such a study is rare in literatures to the authors' knowledge. In WUI-LMD, an ultrasonic impact treatment (UIT) tip is placed near laser spot for in-situ treatment of laser-deposited warm solid material, and the UIT and LMD processes proceed simultaneously. Under the conditions investigated, it is found that in-situ UIT during WUI-LMD can be much more effective in reducing porosity than a post-process UIT. Possible underlying mechanisms are analyzed. WUI-LMD has a great potential to reduce defects and improve mechanical properties without increasing manufacturing time.

scholarly journals Estimating Nitrogen and Chlorophyll Status of Romaine Lettuce Using SPAD and at LEAF Readings

2019 ◽  
Vol 47 (3) ◽  
Author(s):  
Rodrigo Omar MENDOZA-TAFOLLA ◽  
Porfirio JUAREZ-LOPEZ ◽  
Ronald-Ernesto ONTIVEROS-CAPURATA ◽  
Manuel SANDOVAL-VILLA ◽  
Iran ALIA-TEJACAL ◽  
...  
Keyword(s):  
Linear Correlation ◽  
Crop Production ◽  
Chlorophyll Concentration ◽  
Production Cycle ◽  
Digital Object Identifier ◽  
Romaine Lettuce ◽  
Fresh Matter ◽  
Nitrogen Levels ◽  
N Concentration

Nitrogen (N) is an essential nutrient for plant growth and development and is especially important in the production of high quality leafy green vegetables. In this experiment, leaf N concentration, chlorophyll concentration (Chl) and weight above fresh matter (AFM) of romaine lettuce (Lactuca sativa L. var. longifolia) were estimated by correlations between in situ SPAD and atLEAF readings. Lettuce was grown in high tunnels during 42 days and was irrigated at five nitrogen levels: 0, 4, 8, 12 and 16 mEq·L-1 of NO3-, based on the Steiner nutrient solution. The N concentration, Chl concentration and AFM were determined in the laboratory, while SPAD and atLEAF readings were measured in situ weekly. SPAD readings had high, positive and significant linear correlations with N (R2 = 0.90), Chl (R2 = 0.97) and AFM (R2 = 0.98); atLEAF readings had a similar linear correlation with N (R2 = 0.91), Chl (R2 = 0.92) and AFM (R2 = 0.97). Besides, SPAD and atLEAF readings had high, positive, and significant linear correlation (R2 = 0.96). Thus, SPAD and atLEAF meters can be used to non-destructively and accurately estimate the N status of lettuce, in a reliable and quick manner during the crop production cycle. In addition, atLEAF is currently more affordable than SPAD.   ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

Plant Ecophysiology: Linking Pattern and Process—a Review

1997 ◽  
Vol 45 (2) ◽  
pp. 351
Author(s):  
Jann Williams ◽  
Derek Eamus
Keyword(s):  
Plant Ecophysiology ◽  
Elevated Atmospheric Co2 ◽  
Pattern And Process ◽  
Ecological Society ◽  
Technological Advances ◽  
Ecological Patterns ◽  
Underlying Mechanisms ◽  
Underlying Processes

Introduction The Symposium ‘Plant Ecophysiology: Linking Pattern and Process’ was held as part of the 1995 meeting of the Ecological Society of Australia (ESA). The aim of the Symposium was to highlight work that examined mechanisms underlying ecological patterns and linked them to ecological and/or evolutionary processes. Another aim was to expose ecologists to the methods available to examine the mechanistic and functional basis of the organisms and systems under study. Much early ecological research has been concerned with the description and classification of vegetation types, with relatively little effort devoted to understanding the underlying processes that determined distribution. A more quantitative approach based on knowledge of the underlying mechanisms can further improve understanding of systems. This was amply demonstrated in a Symposium on the effects of elevated atmospheric CO2 on vegetation dynamics, also held in conjunction with an ESA meeting (see papers in Australian Journal of Botany, Volume 40(2)). Recent technological advances have stimulated rapid progress in the field of ecophysiology and hence an increasing process-based understanding is developing. The 1995 Symposium was seen as an opportunity to highlight more recent work in what is a relatively new field in Australia (albeit a well-established field in Europe and America), especially in situ studies and research from relatively little studied areas like northern Australia. The response to the Symposium was encouraging, with 25 spoken papers and poster-papers presented. In this paper, some of the unifying aspects of the papers presented in the symposium are drawn together, and placed in the context of likely future developments in ecophysiology in Australia. Based on this analysis, future directions and gaps are identified.

scholarly journals Investigating tool engagement in groundwood pulping: finite element modelling and in-situ observations at the microscale

Holzforschung ◽  
2020 ◽  
Vol 74 (5) ◽  
pp. 477-487 ◽  
Author(s):  
Jenny Carlsson ◽  
Magnus Heldin ◽  
Per Isaksson ◽  
Urban Wiklund
Keyword(s):  
Finite Element ◽  
Numerical Models ◽  
X Ray ◽  
Tool Geometries ◽  
Commercial Use ◽  
X Ray Computed ◽  
In Situ Observations ◽  
Underlying Mechanisms

AbstractWith industrial groundwood pulping processes relying on carefully designed grit surfaces being developed for commercial use, it is increasingly important to understand the mechanisms occurring in the contact between wood and tool. We present a methodology to experimentally and numerically analyse the effect of different tool geometries on the groundwood pulping defibration process. Using a combination of high-resolution experimental and numerical methods, including finite element (FE) models, digital volume correlation (DVC) of synchrotron radiation-based X-ray computed tomography (CT) of initial grinding and lab-scale grinding experiments, this paper aims to study such mechanisms. Three different asperity geometries were studied in FE simulations and in grinding of wood from Norway spruce. We found a good correlation between strains obtained from FE models and strains calculated using DVC from stacks of CT images of initial grinding. We also correlate the strains obtained from numerical models to the integrity of the separated fibres in lab-scale grinding experiments. In conclusion, we found that, by modifying the asperity geometries, it is, to some extent, possible to control the underlying mechanisms, enabling development of better tools in terms of efficiency, quality of the fibres and stability of the groundwood pulping process.

Bradykinin facilitates noradrenaline spillover during contraction in the canine gracilis muscle

2001 ◽  
Vol 79 (10) ◽  
pp. 831-835
Author(s):  
Julie L Lavoie ◽  
Louise Béliveau
Keyword(s):  
Skeletal Muscle ◽  
Muscle Contraction ◽  
Gracilis Muscle ◽  
Bradykinin Receptor ◽  
Hoe 140 ◽  
Underlying Mechanisms

Noradrenaline spillover from skeletal muscle vascular areas increases during exercise but the underlying mechanisms are not well understood. Muscle contraction itself causes changes in many factors that could affect noradrenaline spillover. For instance, it has been reported that bradykinin is synthesized in skeletal muscle areas during contraction. Because the B2 bradykinin receptor facilitates noradrenaline spillover, it may be involved in the increase associated with contraction. In this experiment, we studied the effect of bradykinin on noradrenaline spillover in the in situ canine gracilis muscle, using the specific B2 antagonist HOE 140. The drug did not modify noradrenaline spillover at rest, but did cause a significant decrease during muscle contraction, from 558 to 181 pg·min–1. As reported previously in the literature, fractional extraction of noradrenaline decreased during muscle contraction. This effect was independent of HOE 140 treatment. In light of our results, it seems that bradykinin formation during muscle contraction may play an important part in the observed increase in noradrenaline spillover but does not affect fractional extraction.Key words: skeletal muscle, fractional extraction, stimulation, HOE 140, B2 receptors.

Improving Dryland Crop Simulation by Assimilating Soil Moisture and Vegetation Data

2020 ◽  
Author(s):  
Yang Lu ◽  
Justin Sheffield
Keyword(s):  
Soil Moisture ◽  
Crop Production ◽  
Management Practices ◽  
Water Productivity ◽  
Vegetation Indices ◽  
Screening Method ◽  
Model Performance ◽  
State Parameter ◽  
Aquacrop Model

&lt;p&gt;Global population is projected to keep increasing rapidly in the next 3 decades, particularly in dryland regions of the developing world, making it a global imperative to enhance crop production. However, improving current crop production in these regions is hampered by yield gaps due to poor soils, lack of irrigation and other management practices. Here we develop a crop modelling capability to help understand gaps, and apply to dryland regions where data for parametrizing and testing models is generally lacking. We present a data assimilation framework to improve simulation capability by assimilating in-situ soil moisture and vegetation data into the FAO AquaCrop model. AquaCrop is a water-driven model that simulates canopy growth, biomass and crop yield as a function of water productivity. The key strength of AquaCrop lies in the low requirement for input data thanks to its simple structure. A global sensitivity analysis is first performed using the Morris screening method and the variance-based Extended Fourier Amplitude Sensitivity Test (EFAST) method to identify the key influential parameters on the model outputs. We begin with state-only updates by assimilating different combinations of soil moisture and vegetation data (vegetation indices, biomass, etc.), and different filtering/smoothing assimilation strategies are tested. Based on the state-only assimilation results, we further evaluate the utility of joint state-parameter (augmented-states) assimilation in improving the model performance. The framework will eventually be extended to assimilate remote sensing estimates of soil moisture and vegetation data to overcome the lack of in-situ data more generally in dryland regions.&lt;/p&gt;

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