scholarly journals Zoledronic acid and teriparatide have a complementary therapeutic effect on aseptic loosening in a rabbit model

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Peng Wang ◽  
Guang-qian Shang ◽  
Shuai Xiang ◽  
Hai-ning Zhang ◽  
Ying-zhen Wang ◽  
...  
Keyword(s):  
Zoledronic Acid ◽  
Aseptic Loosening ◽  
Rabbit Model ◽  
Acid Group ◽  
Control Group ◽  
Periprosthetic Osteolysis ◽  
The Stability ◽  
The Right ◽  
Masson Trichrome Staining

Abstract Background Revisions are mainly caused by wear debris-induced aseptic loosening. How to effectively suppress debris-induced periprosthetic osteolysis has become an urgent problem. Both zoledronic acid and teriparatide can increase the bone mass around prostheses and increase the stability of prostheses. A hypothesis was proposed: the combination of the two drugs may have a better treatment effect than the use of either drug alone. Methods We created a rabbit model to study the effect and mechanism of the combination of zoledronic acid and teriparatide in the treatment of aseptic loosening. Thirty-two adult male New Zealand white rabbits were selected and treated with TKA surgery, and a titanium rod prosthesis coated evenly with micrometre-sized titanium debris was implanted into the right femoral medullary cavity. All rabbits were randomized into four groups (control group = 8, zoledronic acid group = 8, teriparatide group = 8, and zoledronic acid + teriparatide group = 8). All the animals were sacrificed in the 12th week, and X-ray analyses, H&E staining, Goldner-Masson trichrome staining, von Kossa staining, and RT-PCR and Western blotting of the mRNA and protein of OCN, OPG, RANKL and TRAP5b in the interface membrane tissues around the prostheses were immediately carried out. Results The results shown that both zoledronic acid and teriparatide could inhibit debris-induced peri-prosthetic osteolysis and promote new bone formation. Zoledronic acid was more capable of inhibiting osteoclast activation and peri-prosthetic osteolysis, while teriparatide was more capable of promoting osteoblast function and peri-prosthetic bone integration. Conclusion This research confirmed that the combination of zoledronic acid and teriparatide could prevent and treat aseptic loosening of the prosthesis more effectively. However, the safety of this combination and the feasibility of long-term application have not been ensured, and the clinical application requires further experiments and clinical research support.

scholarly journals The study of the impacts of ‘Running’ on the contact area of soles and maximal strength among elite middle distance runners

2017 ◽  
Vol 12 (1) ◽  
pp. 23-31 ◽  
Author(s):  
Ahmet Uzun ◽  
Latif Aydos ◽  
Metin Kaya ◽  
Mehmet Fatih Yuksel ◽  
Hacı Ahmet Pekel
Keyword(s):  
Contact Area ◽  
Middle Part ◽  
Control Group ◽  
Maximal Strength ◽  
Distance Runners ◽  
Male Athletes ◽  
Maximal Force ◽  
Analysis System ◽  
The Right

It is possible that running training for many years in athletics affects athletes' running patterns and sole structure. The main aim of this study is to examine the effect of maximal force applied to the floor area and contact area of the athletes with related to mid-distance training for athletics. 18 male athletes who represent Turkey on the International area and another 25 male volunteers who form the control group from the participants of the study. The sole pressure measurement of the participants was performed through the use of EMED-SF (Novel H, Munich, Germany) plantar pressure analysis system (pedobarographic analysis).  In the study, significant differences were found in heel medial, heel lateral, foot middle, 2nd, 3rd and 4th metatars heads and left foot 3rd, 4th, 5th finger in the contact area values of the right and left foot averages. Besides, significant differences were found in heel medial, middle part of feet, 2nd and 3rd metatars heads and 2nd, 3rd, 4th and 5th fingers in maximal force values. Athletes have pressed on sole of the feet more as a result of long-term training and as a consequence this pressure caused a change of the soles contact area and maximal strength. Also it can be said that they have used forefront part of soles more actively during running and walking.   Keywords: runner foot sole, contact area, middle distance, pedobarographic.

scholarly journals Evaluation of long-term stability of mesiodistal axial inclinations of maxillary molars through panoramic radiographs in subjects treated with Pendulum appliance

2016 ◽  
Vol 21 (1) ◽  
pp. 67-74 ◽  
Author(s):  
Caroline Andrade Rocha ◽  
Renato Rodrigues de Almeida ◽  
José Fernando Castanha Henriques ◽  
Carlos Flores-Mir ◽  
Marcio Rodrigues de Almeida
Keyword(s):  
Panoramic Radiograph ◽  
Post Treatment ◽  
Control Group ◽  
Long Term Stability ◽  
Maxillary Molars ◽  
Group Data ◽  
Maxillary Molar ◽  
Pendulum Appliance ◽  
The Stability

Objective: To evaluate the stability of mesiodistal inclination of maxillary molars produced by a pendulum appliance, five years after completion of orthodontic treatment. Angulation changes were compared to an untreated sample. Methods: The sample consisted of 20 patients (14 females and 6 males) with Class II, Division 1 malocclusion that was treated through molar distalization with a pendulum appliance followed by cervical headgear and full fixed appliances. Maxillary molar inclination was evaluated through panoramic radiograph. The mean age at pretreatment was 14.3 ± 1.6 years, whereas at immediate post-treatment it was 18.6 ± 1.8 years, and at long-term post-treatment it was 23.8 ± 2.0 years. A control group of 16 untreated individuals with untreated normocclusion ranging in age from 12 to 17 years old were used as comparison group. Data were statistically analyzed with independent t-tests and ANOVA test followed by Tukey post-hoc tests. Results: Statistically significant differences were found between T1 (94.50) and T2 (98.80) as well as between T2 and T3 (94.70) for maxillary first molars. Maxillary second molars did not show any statistically significant positional changes during the evaluated time periods T1 (107.50), T2 (109.30) and T3 (106.90). Conclusion: Although maxillary first molars underwent distal crown inclination immediately after treatment, approximately five years thereafter their roots tended to upright close to the pretreatment positions.

scholarly journals Micrometer-Sized Titanium Particles Induce Aseptic Loosening in Rabbit Knee

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Hao Xu ◽  
Cui-cui Guo ◽  
Zheng-yu Gao ◽  
Chang-yao Wang ◽  
Hai-ning Zhang ◽  
...  
Keyword(s):  
Aseptic Loosening ◽  
Bone Ingrowth ◽  
Osteoclast Differentiation ◽  
Rabbit Model ◽  
Medullary Cavity ◽  
Inflammatory Factors ◽  
Control Group ◽  
Titanium Particle ◽  
Effective Prevention ◽  
Rabbit Knee

Wear debris induced aseptic loosening is the leading cause of total knee arthroplasty (TKA) failure. The complex mechanism of aseptic loosening has been a major issue for introducing effective prevention and treatment methods, so a simplified yet efficient rabbit model was established to address this concern with the use of micrometer-sized titanium particles. 20 New Zealand white rabbits were selected and divided into two groups (control = 10, study = 10). A TKA surgery was then performed for each of them, with implantation of a titanium rod prosthesis which was coated evenly with micrometer-sized titanium in the study group and nothing in the control group, into right femoral medullary cavity. After 12 weeks, all the animals were euthanized and X-ray analyses, H&E staining, Goldner Masson trichrome staining, Von Kossa staining, PCR, and Western blotting of some specific mRNAs and proteins in the interface membrane tissues around the prosthesis were carried out. The implantation of a titanium rod prosthesis coated with 20 μm titanium particles into the femoral medullary cavity of rabbits caused continuous titanium particle stimulation around the prosthesis, effectively inducing osteolysis and aseptic loosening. Titanium particle-induced macrophages produce multiple inflammatory factors able to activate osteoclast differentiation through the OPG/RANKL/RANK signaling pathway, resulting in osteolysis while suppressing the function of osteoblasts and reducing bone ingrowth around the prosthesis. This model simulated the implantation and loosening process of an artificial prosthesis, which is an ideal etiological model to study the aseptic prosthetic loosening.

scholarly journals Effects of Bisphosphonates on Osseointegration of Dental Implants in Rabbit Model

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Ru Qing Yu ◽  
Jing Yi Wang ◽  
Nian Jing Rao ◽  
Lei Huo ◽  
Li Wu Zheng
Keyword(s):  
Zoledronic Acid ◽  
Dental Implants ◽  
Dental Implant ◽  
Growth Rates ◽  
Acid Treatment ◽  
Bone Growth ◽  
Rabbit Model ◽  
Short Term ◽  
Calvarial Bone

This study is to investigate the effect of bisphosphonates on the osseointegration of dental implants in a rabbit model. Twenty female New Zealand White rabbits were equally assigned into control and experiment groups which received saline or zoledronic acid treatment 4 weeks prior to surgery. Titanium dental implant was placed on the calvarial bone. Zoledronic acid or saline treatment continued after surgery for 4 weeks (short-term subgroup) or 8 weeks (long-term subgroup) until sacrifice. Three different fluorochrome labeling solutions were administrated for assessing bone growth rates. Samples of the calvarial bone and mandible were subjected to microcomputed tomography (micro-CT), confocal microscope, and histology analysis. Zoledronic acid treatment significantly reduced bone growth rates in the calvarial bone, but had no significant influence in bone mineral density and trabecular microarchitecture. Significantly lower bone-to-implant contact ratios were found in zoledronic acid-treated animals compared to controls at week 4 but not at week 8. Oncologic dose zoledronic acid suppresses the bone growth rates of the calvarial bone; ZA may have an adverse effect on osseointegration of dental implant in short term, but this effect tends to diminish in long term.

scholarly journals The Effect of Intravitreal Azithromycin on the Albino Newborn Rabbit Retina

2016 ◽  
Vol 10 (1) ◽  
pp. 12-16
Author(s):  
Duygu Cam ◽  
Ali Osman Saatci ◽  
Serap Cilaker Micili ◽  
Bekir Ugur Ergur ◽  
Revan Yildirim Karabag ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Caspase 3 ◽  
Rabbit Model ◽  
Study Groups ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Control Group ◽  
Tunel Staining ◽  
Rabbit Retina ◽  
Newborn Rabbit ◽  
The Right

Purpose: To evaluate the effect of intravitreal azithromycin on the retina in a newborn rabbit model. Methods: Twelve, two-week old New Zealand albino rabbits were divided into two groups (six in each). The right eyes of six rabbits received 0.75 mg (0.05 mL) azithromycin and the right eyes of the remaining six rabbits 1.5 mg (0.1 mL) azithromycin intravitreally. Left eyes were served as the control and received the same volume of saline. All eyes were enucleated at the third postinjection week. Retinal histology was examined by light microscopy. Apoptosis of the retinal cells was further evaluated by immunohistochemical staining for caspase-3 and in situ terminal deoxynucleotidyl transferase-mediated biotin-deoxyuridine triphosphate nick-end labeling (TUNEL) of DNA fragments. Results: Light microscopy demonstrated no retinal abnormalities in all eyes. However, retinal nuclear DNA fragmentation was evident in both study groups (33.6% with 1.5 mg and 21.4% with 0.75 mg azithromycin) with the TUNEL method. TUNEL staining ratio was statistically higher only in the second group treated with 1.5 mg azithromycin when compared to the control group (p=0.01 Mann Whitney U test). The ratio of caspase-3 positive cells in the two study groups was 21.5% and 20.2%, respectively. Caspase-3 staining ratio was statistically higher in both study groups when compared to the control eyes (p=0.00, p=0.00 respectively). The difference of TUNEL staining ratio between the two study groups was statistically significant (p=0.028), but there were no statistically significant differences in the two study groups by caspase-3 staining (p=0.247). Conclusion: In newborn rabbits, intravitreal azithromycin injection resulted in an apoptotic activity in the photoreceptor, bipolar and ganglion cells. Immunohistochemical analysis suggested that doses of 0.75 mg and 1.5 mg azithromycin, administered intravitreally might be toxic to the newborn rabbit retina.

scholarly journals Does demineralized bone matrix enhance tendon-to-bone healing after rotator cuff repair in a rabbit model?

2019 ◽  
Author(s):  
Woo-Yong Lee ◽  
Young-Mo Kim ◽  
Hyun-Dae Shin ◽  
Deuk-Soo Hwang ◽  
Yong-Bum Joo ◽  
...  
Keyword(s):  
Rotator Cuff ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Rabbit Model ◽  
Longitudinal Direction ◽  
Collagen Fibers ◽  
Control Group ◽  
The Right ◽  
Demineralized Bone

Abstract Background The purpose of this study was to compare the histologic outcomes after rotator cuff (RC) repair between with demineralized bone matrix (DBM) augmentation and without DBM and to evaluate the role of DBM for tendon-to-bone (TB) healing in a rabbit model. Methods Twenty-six adult male New Zealand white rabbits were randomly allocated to the control group (n = 13) or the DBM group (n = 13). A chronic RC tear was generated on the right shoulder of all rabbits. In the control group, RC repair was achieved by a standard transosseous technique. In the DBM group, RC repair was achieved using the same technique, and DBM was interposed between the cuff and bone. After 8 weeks, the RC tendon entheses from all rabbits were processed for gross and histologic examination. Results In the control group, the tendon midsubstance was disorganized with randomly and loosely arranged collagen fibers and rounded fibroblastic nuclei. The TB interface was predominantly fibrous with small regions of fibrocartilage, especially mineralized fibrocartilage. In the DBM group, the tendon midsubstance appeared normal and comprised densely arranged collagen fibers, with orientated crimped collagen fibers running in the longitudinal direction of the tendon. These fibers were interspersed with elongated fibroblast nuclei. The TB interface consisted of organized collagen fibers with large quantities of fibrocartilage and mineralized fibrocartilage. Conclusion DBM augmentation at the RC-to-bone interface enhances TB healing after RC repair.

scholarly journals Cardiac Changes in Patients on Long-Term Parenteral Nutrition

Nutrients ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1587 ◽  
Author(s):  
Lidia Santarpia ◽  
Roberta Esposito ◽  
Andrea Vaccaro ◽  
Lucia Alfonsi ◽  
Maria Carmen Pagano ◽  
...  
Keyword(s):  
Parenteral Nutrition ◽  
Superior Vena ◽  
Vena Cava ◽  
Venous Catheter ◽  
Control Group ◽  
High Fluid ◽  
The Right ◽  
And Function

Patients with short bowel syndrome (SBS) on long-term home parenteral nutrition (HPN) chronically receive high fluid volumes directly into the right atrium (RA) through the superior vena cava. We retrospectively evaluated cardiac function measured by routine transthoracic echocardiography (TTE) in a population of 26 SBS patients on long-term HPN and compared their data on echocardiograph-derived right heart structure and function, with those of a control group of 26 patients also bearing a central venous catheter (CVC) for other reasons. Results showed that body weight and BMI were significantly higher in the control group. The echocardiographic estimate of RA pressure was higher in HPN patients than in controls (p = 0.01). An increased estimate of RA pressure indicates the need to consider TTE in the follow-up of long-term HPN patients to detect functional impairment early.

scholarly journals Sclerotic Effect of Oxytetracycline on the Submandibular Gland: An Experimental Model

2016 ◽  
Vol 95 (12) ◽  
pp. 492-496
Author(s):  
Oğuz Güçlü ◽  
Asli Muratli ◽  
Deniz Arik ◽  
Kazim Tekin ◽  
Halil Erdogan ◽  
...  
Keyword(s):  
Submandibular Gland ◽  
Sham Group ◽  
Active Treatment Group ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Control Group ◽  
Alternate Therapy ◽  
New Zealand White Rabbits ◽  
Hematoxylin And Eosin ◽  
The Right ◽  
Masson Trichrome Staining

Oxytetracycline has been suggested as an alternate therapy for chronic recurrent sialadenitis and sialorrhea. We conducted an experimental study to investigate the sclerotic effect of this drug on the submandibular gland by histopathologic methods. Our subjects were 20 New Zealand white rabbits, which were divided into two groups of 10. The right submandibular gland of the rabbits in the active-treatment group was injected with 0.3 ml of oxytetracycline (100 mg/ml), and that of the controls was injected with saline. Four weeks after the injections, all the glands were removed. Histopathologic studies, including hematoxylin and eosin and Masson trichrome staining, were carried out. The glands were evaluated for tissue inflammation, congestion, fibrosis, edema, lipomatosis, and atrophy. To investigate apoptosis, terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick-end labeling (TUNEL) immunohistochemical staining was used. In the study group, inflammation (n = 9), congestion (n = 9), fibrosis (n = 6), edema (n = 6), and lipomatosis (n = 4) were observed; in the sham group, only lipomatosis was seen (n = 5). The TUNEL assay results for acinar cells were 4.51 ± 1.41% in the oxytetracycline group and 2.08 ± 1.76% in the control group (p = 0.006); the corresponding figures for the duct cells were 7.05 ± 0.87% and 3.10 ± 2.26% (p = 0.001). Based on our findings, we conclude that oxytetracycline might be a viable alternative for the treatment of chronic recurrent sialadenitis and sialorrhea. However, more research in this area is needed.

scholarly journals Effects of Zoledronic Acid on Physiologic Bone Remodeling of Condylar Part of TMJ: A Radiologic and Histomorphometric Examination in Rabbits

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Ufuk Tatli ◽  
Yakup Üstün ◽  
Mehmet Kürkçü ◽  
Mehmet Emre Benlidayı
Keyword(s):  
Single Dose ◽  
Zoledronic Acid ◽  
Bone Remodeling ◽  
Infusion Pump ◽  
Rabbit Model ◽  
Mandibular Condyle ◽  
Control Group ◽  
New Zealand White Rabbits ◽  
Base Data ◽  
Experimental Group

Objective. The purpose of the present study is to evaluate the effects of systemically administered zoledronic acid (ZA) on the physiological bone remodeling and the microarchitectural parameters of the condylar part of TMJ in a rabbit model.Study Design. Thirty skeletally mature male New Zealand white rabbits were randomly divided into two groups. The experimental group was administered an intravenous, single dose of 0.1 mg/kg ZA diluted with 15 mL of saline in a 15-minute perfusion with an infusion pump. The control group was administered only saline infusion for 15 minutes. All rabbits were sacrificed on the 21st postoperative day. Radiodensitometric and histomorphometric examinations were performed on the harvested mandibular condyles. The data were analyzed statistically.Results. Radiodensitometric findings showed that ZA treatment resulted in a significant increase in the mineralization of mandibular condyle. This result was supported by the histomorphometric findings.Conclusion. The present study has revealed that a temporary delay in the physiological bone remodeling using single dose of ZA increases bone mineral content and makes the microarchitecture of the mandibular condyle more compact. These effects may be regarded as base data and considered in numerous clinical situations including TMJ.

Stability of Implicit Self-Esteem Among Internetaddicted College Students in China

2017 ◽  
Vol 45 (2) ◽  
pp. 339-352 ◽  
Author(s):  
Lingbo Zhao ◽  
Xianglian Yu ◽  
Lixian Zhang ◽  
Zhihong Ren
Keyword(s):  
College Students ◽  
Internet Addiction ◽  
Positive Feedback ◽  
Self Esteem ◽  
Group Differences ◽  
The Internet ◽  
Control Group ◽  
Long Term Stability ◽  
The Stability

We explored the stability of, and between-group differences in, the implicit self-esteem of Internet-addicted and Internet-nonaddicted college students in China. Internet addiction was assessed using Young's Internet Addiction Scale and the participants comprised 52 Internet addicts (26 men, 26 women) and 52 Internet nonaddicts (26 men, 26 women). We then used the Implicit Association Test to assess levels of implicit self-esteem, employing a 2 (addiction, nonaddiction) × 2 (positive feedback, negative feedback) between-subjects factorial design. The pretest implicit self-esteem level of the Internet-addicted group was significantly higher than that of the control group; however, the implicit self-esteem levels of both groups were unstable. Positive feedback did not change the level of implicit self-esteem of the Internet addiction group. In future studies researchers need to pay more attention to the measurement of implicit self-esteem, long-term stability, and addiction to online games.

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