scholarly journals WT1 and ACE mRNAs of blood extracellular vesicle as biomarkers of diabetic nephropathy

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Ehsan Hashemi ◽  
Hojat Dehghanbanadaki ◽  
Alireza Abbasi Baharanchi ◽  
Katayoon Forouzanfar ◽  
Ardeshir Kakaei ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Roc Analysis ◽  
Mrna Level ◽  
Extracellular Vesicle ◽  
Cross Sectional Study ◽  
Fold Change ◽  
Diabetic Patients ◽  
Healthy Controls ◽  
Cross Sectional ◽  
Intercellular Communications

Abstract Background Diabetic nephropathy (DN) has an increasing global prevalence with excessive health expenditure and burden. Exosomal mRNAs regulate intercellular communications and participate in the pathogenesis of various disorders like DN. This study aimed to assess the expression levels of ACE, ELMO1, and WT1 mRNAs in the blood extracellular vesicles (EVs) of DN patients and diabetic patients without nephropathy (DM group) in comparison to healthy controls and investigate their correlations with the severity of DN. Methods The performed investigation is a cross-sectional study of 256 participants including 103 DN patients, 100 DM patients, and 53 healthy controls. The quantification of WT1, ACE, and ELMO1 mRNAs in the blood EVs were executed using qRT-PCR. The ROC analysis was performed to determine the diagnostic accuracy of mRNAs. Results DN patients had significantly higher expressed WT1 mRNA (1.70-fold change) and lower expressed ACE mRNA (0.55-fold change) in the blood EVs compared to DM patients and controls. ELMO1 mRNA was not expressed in EVs of any groups. A positive correlation between WT1 mRNA level and urine Alb/Cr ratio (r = 0.602, p < 0.001) and a negative correlation between ACE mRNA expression and urine Alb/Cr ratio within DN patients (r = − 0.474, p < 0.001) was identified. The accuracy of WT1 mRNA and 1/ACE mRNA for predicting incipient DN was 0.63 (95% CI 0.55, 0.72) and 0.62 (95% CI 0.54, 0.71), and for predicting overt DN was 0.83 (95% CI 0.74, 0.92) and 0.75 (95% CI 0.66, 0.83), respectively. Conclusions WT1 and ACE mRNAs level in blood EVs were predictors for early diagnosis of DN therefore their quantifications might be used to determine the severity of albuminuria and glomerular injuries.

scholarly journals Sarcopenia in diabetic nephropathy: a cross-sectional study

2018 ◽  
Vol 56 (2) ◽  
pp. 102-108 ◽  
Author(s):  
Meral Çeliker ◽  
Mustafa Yavuz Selçuk ◽  
Serdar Olt
Keyword(s):  
Diabetic Nephropathy ◽  
Cross Sectional Study ◽  
Control Group ◽  
Diabetic Patients ◽  
Healthy Controls ◽  
Diabetes Group ◽  
Cross Sectional ◽  
Hand Grip ◽  
European Working ◽  
Healthy Control

Abstract Objective. To investigate the relationship between sarcopenia and diabetic nephropathy. Methods. 56 diabetic patients without complications, 50 diabetic patients with nephropathy, 53 healthy controls included in this present study. Demographic characteristics such as sex, age, anthropometric measurements such as weight, body mass index [BMI], hip circumference, waist circumference and upper arm circumference were measured. Sarcopenia diagnosis was based on European Working Group on Sarcopenia in Older People [EWGSOP] criteria which consist of hand grip strength, 6-meter walking test and muscle mass. Results. The frequency of sarcopenia increased gradually from 15.1% in healthy control group to 21.4% in the diabetes group, and 34% in diabetic nephropathy group (X2 for trend, p = 0.029). The frequency of sarcopenia was similar in diabetes and diabetic nephropathy group. However, the frequency of sarcopenia was higher in diabetic nephropathy than healthy controls (OR = 2.89, CI [1.11-7.51] in logistic regression). Conclusion: In the present study, the prevalence of sarcopenia was higher in patients with diabetic nephropathy compared to healthy controls.

scholarly journals A study to assess the correlation between HbA1c and microalbuminuria among diabetics

2020 ◽  
Vol 8 (1) ◽  
pp. 16
Author(s):  
Sruthi Kare ◽  
Vishwanath N. Reddy ◽  
Thejdeep Mahamkali
Keyword(s):  
Diabetes Mellitus ◽  
Diabetic Nephropathy ◽  
Socioeconomic Development ◽  
Cross Sectional Study ◽  
Diabetic Patients ◽  
Cross Sectional ◽  
Duration Of Diabetes ◽  
Medical College ◽  
Significant Linear Correlation ◽  
Patients With Diabetes

Background: India is one of the epicentres of the global diabetes mellitus pandemic. Rapid socioeconomic development and demographic changes, along with increased susceptibility for Indian individuals, have led to the explosive increase in the prevalence of diabetes mellitus in India over the past four decades. Diabetic Nephropathy is a common consequence of long-standing diabetes mellitus. The development of diabetic nephropathy has a dramatic increase on the morbidity and mortality of patients with diabetes. Objective of this study was to evaluate the prevalence of microalbuminuria in patients with diabetes mellitus patients.  Methods: This cross-sectional study was conducted on T 2 diabetes mellitus patients visiting medicine OPD of R L Jalappa hospital constituent hospital of Sri Deveraj Urs Medical College, Tamaka, Kolar from May 2016 to July 2016. A total of 60 type-2 diabetes patients were enrolled in the study.Results: Average duration of diabetes among study group was 8 years and most of the patients were between 6-10 years. In type 2DM patients, microalbuminuria and glycemic control have shown a significant linear correlation with duration of diabetes (p<0.05). Also, micro albuminuria has a significant correlation with increase in level of glycosylated haemoglobin.  Conclusions: The prevalence of microalbuminuria in diabetic patients was found to be high and being a developing country; there is a dire need that microalbuminuria and HbA1c testing should be done in both, newly diagnosed as well as already diagnosed type 2DM patients as an early marker of renal risk factor.

scholarly journals THE CORRELATION BETWEEN THE MEAN PLATELET VOLUME VALUES WITH THROMBOCYTE AGGREGATION IN NEPHROPATHY DIABETIC PATIENTS

2019 ◽  
Vol 25 (1) ◽  
pp. 79
Author(s):  
Agus Sunardi ◽  
Nadjwa Zamalek Dalimoenthe ◽  
Coriejati Rita ◽  
Adhi Kristianto Sugianli
Keyword(s):  
Diabetic Nephropathy ◽  
Platelet Aggregation ◽  
Mean Platelet Volume ◽  
Cross Sectional Study ◽  
Endothelial Damage ◽  
Diabetic Patients ◽  
Platelet Volume ◽  
Cross Sectional ◽  
Positive Correlation ◽  
Chronic Hyperglycemia

     Diabetic nephropathy is the most important cause of end-stage renal failure. Chronic hyperglycemia will cause glomerular endothelial damage, and this damage will stimulate hemostasis activation including platelets so that platelet aggregation will increase. The increase of platelet aggregation will increase platelet consumption, which further stimulates thrombopoiesis which will lead to immature platelets of large size to be released into the circulation. This research aimed to determine the positive correlation between MPV with platelet aggregation in patients with diabetic nephropathy. This study was an analytic observational study with a cross-sectional study design. The research was conducted in the Dr. Hasan Sadikin Hospital Bandung from July 2016 to October 2017. A total of 52 subjects who met the inclusion criteria were included in the study. Mean platelet volume and platelet aggregation were performed with venous examination with EDTA and sodium citrate 3.2% anticoagulants. The result of platelet aggregation examination showing platelet hyper-aggregation was found in 44.2% of subjects, 50% normal-aggregation, 5.8% hypo-aggregation. While the median value of MPV in this study was 9.2 fL with the range of 8.00 – 11.80 fL. A positive correlation was found  between MPV value with platelet aggregation with r= 0.067, p= 0.634. The conclusion was that there was no correlation between MPV values with platelet aggregation in diabetic nephropathy patients. This small and insignificant r-value might be due to several factors that also affect platelet aggregation in diabetic nephropathy patients, requiring further investigation.

scholarly journals Diabetic nephropathy as a cause of end-stage renal disease in Egypt: a six-year study

2004 ◽  
Vol 10 (4-5) ◽  
pp. 620-626 ◽  
Author(s):  
A. Afifi ◽  
M. El Setouhy ◽  
M. El Sharkawy ◽  
M. Ali ◽  
H. Ahmed ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Renal Disease ◽  
End Stage Renal Disease ◽  
Large Scale ◽  
Cross Sectional Study ◽  
Diabetic Patients ◽  
Cross Sectional ◽  
Stage Renal Disease ◽  
End Stage ◽  
Esrd Patients

The prevalence of diabetic nephropathy as a cause of end-stage renal disease [ESRD] in Egypt has been examined in small cross-sectional studies, with conflicting results. The need for a large-scale study prompted us to perform this 6-year multiple cross-sectional study. A sample of ESRD patients enrolled in the Egyptian renal data system was evaluated during the period 1996-2001 for the prevalence of diabetic nephropathy. Prevalence gradually increased from 8.9% in 1996, to 14.5% in 2001. The mean age of patients with diabetic nephropathy was significantly higher than that of patients with ESRD from other causes. Mortality was also significantly higher in diabetic patients with ESRD

scholarly journals Microalbuminuria in Patients with Diabetes Mellitus: Prevalence and Associated Risk factors Title of your

2017 ◽  
Vol 2 (2) ◽  
pp. 155-159
Author(s):  
Zhian S. Ramzi ◽  
Mahfoodh A. Sulaiman
Keyword(s):  
Risk Factors ◽  
Diabetic Nephropathy ◽  
Glycemic Control ◽  
Cross Sectional Study ◽  
Poor Glycemic Control ◽  
Renal Diseases ◽  
Diabetic Patients ◽  
Cross Sectional ◽  
And Gender ◽  
Associated Risk Factors

Microalbuminuria is the most leading cause for cardiovascular disease, diabetic nephropathy, end stage renal diseases, and death among diabetic patients. The study carried out                                                                                       to find out the prevalence of microalbuminuria in a sample of Sulaimani diabetics and to determine its relation to different associated risk factors. The first part of study was a cross-sectional study conducted through examination of records of diabetic patients registered in Sulaimani Diabetic Center from June 2010 to May 2012. The second part was a case- control study that conducted in the same center, including 50 cases of diabetics with positive microalbuminuria, and 50 controls with negative one, that were registered in the same center and matched by age and gender. A specially designed questionnaire was used by researcher to collect information from the records. SPSS version 21.0 was used for data analysis. Males accounts for half of cases and controls, highest proportion of sample was found among age group (60-69) years about 34%. The prevalence of microalbuminuria was 29%. The study showed a statistical significant association of microalbuminuria with obesity, smoking, hypertension, retinopathy, ischemic heart disease, poor glycemic control, and dyslipidemia.  In conclusion moderate prevalence of microalbuminuria was found among diabetic patients, the prevalence was more common in poor glycemic control, and hypertensive diabetics. There was significant relation between microalbuminuria and diabetic nephropathy.

scholarly journals Plasma Cyclophilin A as a Novel Biomarker in Chronic Nephropathy

2020 ◽  
pp. 63-72
Author(s):  
Mohy Eldin Abd EL-Fattah ◽  
Taghrid B. El-abaseri ◽  
Hegazy Mohamed Abd Elaziz Mohamed
Keyword(s):  
Diabetic Nephropathy ◽  
Cyclophilin A ◽  
Cross Sectional Study ◽  
Control Group ◽  
Diabetic Patients ◽  
Cross Sectional ◽  
Stage Renal Disease ◽  
End Stage ◽  
G Ratio

Background: Type 2 diabetes mellitus (DM) is the most common cause of end- stage renal disease. Albuminuria is the foremost commonly utilized marker to anticipate onset of diabetic nephropathy (DN) without sufficient affectability and specificity to identify early DN. Aim: This study aimed to evaluate Plasma cyclophilin A (CypA) as a new biomarker for early DN. Methods: This cross sectional study included 125 Egyptian subjects attending the out Patients Clinic of the Department of Internal Medicine, 10Th of Ramadan city Health Insurance Hospital and divided into-:control group, patient with diabetic mellitus, patients with Diabetic nephropathy and patient with diabetic nephropathy and other complications. Patients were subjected to measurement of plasma cyclophyline A, FBS, HbAIC, serum creatinine, serum urea, serum uric acid, k, Na, serum phosphorus, Albumin:Creatinine Ratio, GFR, Chol, TG, LDL HDL, AST, ALT, T.BIL, D.BIL ALB, TP, GLB and A/G ratio. Results: Results showed that Cyclophilin A was significantly correlated with duration of DM, CR, Urea, UR.A, Na, phosphorus, ACR, Chol, TG, LDL, AST, ALT, T.BIL, D.BIL. Meanwhile, Cyclophilin A was negatively correlated with HA1C, K, GFR, HDL, ALB, TP, GLB and A/G ratio. At cut-off level ≥84.14, cyclophilin A had 91% sensitivity and 62% specificity for diagnosing diabetic nephropathy. Conclusion: CypA can be used as an early marker for DN as we found early significant high levels of urinary CypA in diabetic patients with stage 2 DN even before the appearance of albuminuria.

Frequency of Renal Diseases in Diabetic Patients

2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Misbah Arshad ◽  
Mamoona Ashfaq ◽  
Zainab Sharmeen ◽  
Zargham Mazhar ◽  
Kashifa Ehsan
Keyword(s):  
Diabetes Mellitus ◽  
Diabetic Nephropathy ◽  
Kidney Disease ◽  
Renal Disease ◽  
Age Groups ◽  
Cross Sectional Study ◽  
Renal Diseases ◽  
Diabetic Patients ◽  
Protein Loss ◽  
Cross Sectional

Diabetic nephropathy, also known as diabetic kidney disease is the chronic loss of kidneyfunction occurring in those with diabetes mellitus. Diabetic nephropathy is one of the leading causes ofchronic kidney disease (CKD) and end-stage renal disease (ESRD) globally. Protein loss in the urine due todamage to the glomeruli may become massive, and cause a low serum albumin with resulting generalizedbody swelling (edema) and result in the nephrotic syndrome. Objective: The aim of this study was todetermine the frequency of renal disease in diabetic patients and its complications in Pakistan.Methods: A cross sectional study was conducted at Renal and Diabetic Departments of the Sir GangaRam Hospital, Lahore, over a period of 3 months, after obtaining the ethical approval from The Universityof Lahore. A total number of 100 Diabetic patients were selected through non probability convenientsampling technique. Patients of both sexes and all age groups were included. Results: In this study 60%were male and 40% were female. About 41% diabetic patients were 1-6 month of age, 42% were 1-5 yearsold and 1% of 18-23 years old who had renal diseases while 9% patients were without any renal disease.whereas the prevalence of diabetes is more in infants than others which is 35%. But there was notsignificant association between onset of renal diseases with the onset of diabetes mellitus with p-value0.24.Conclusions: Results of current study showed that diabetes mellitus effecting individuals of all agesequally but there was not significant association between diabetes and renal diseases.

scholarly journals DIABETIC RETINOPATHY IN PATIENTS OF TYPE2 DIABETES MELLITUS WITH DIABETIC NEPHROPATHY

2020 ◽  
pp. 1-2
Author(s):  
Somedeb Gupta
Keyword(s):  
Diabetes Mellitus ◽  
Diabetic Retinopathy ◽  
Diabetic Nephropathy ◽  
Macular Edema ◽  
Cross Sectional Study ◽  
Diabetic Patients ◽  
Cross Sectional ◽  
Female Ratio ◽  
Clinically Significant

To evaluate severity of diabetic retinopathy in patients of type 2 diabetes mellitus with diabetic nephropathy. This was a cross sectional study of 159 eyes of 80 patients aged above 40 years, diagnosed to have Type 2 DM with diabetic nephropathy. All the patients were investigated for albuminuria. The retinopathy was evaluated according to the Early Treatment Diabetes Retinopathy Study (ETDRS) classification into nonproliferative diabetic retinopathy (NPDR) and proliferative diabetic retinopathy (PDR). Diabetic macular edema was characterized as clinically significant macular edema (CSME) and non-CSME. The severity of retinopathy was correlated with nephropathy. In this study, mean age of the patients were 58.26± 6.43 years and male to female ratio 1.96:1. Out of 80 diabetic patients, 22(27.5%) patients had microalbuminuria and 58 (72.5 %) had macroalbuminuria. In this study, 67 (83.7%) patients had diabetic retinopathy, out of which 14 (20.9%) patients had mild NPDR, 20 (29.9%) had moderate NPDR, 16 (23.9%) had severe NPDR, 5(7.4%) had very severe NPDR and 12 (17.9%) had PDR. In 22 patients with microalbuminuria 6 (27.3%) had mild NPDR, 4 (5.0%) had moderate NPDR and none had maculopathy. In 58 patients with macroalbuminuria, 8 (13.8%) had mild NPDR, 16 (27.6%) had moderate NPDR, 16 (27.6%) severe NPDR, 5 (8.6%) has very severe NPDR, 4(6.9%) had early PDR and 8(13.8%) had high-risk PDR, 14 (24.1%) had CSME and 4 (6.9%) had non-CSME. In our study, diabetic nephropathy patients with macroalbuminuria had more severe type of DR than patients with microalbuminuria.

scholarly journals STUDY OF PREVALENCE OF NEPHROPATHY IN NEWLY DIAGNOSED TYPE 2 DIABETES MELLITUS PATIENTS

2020 ◽  
pp. 1-2
Author(s):  
Abhijeet Kuma ◽  
Pankaj Hans ◽  
Ram Raj Ravi
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Diabetic Nephropathy ◽  
Cardiovascular Complications ◽  
Cross Sectional Study ◽  
Diabetic Patients ◽  
Newly Diagnosed ◽  
Cross Sectional

INTRODUCTION: Diabetes Mellitus is one of the commonest diseases of the world.The dangerous fact about diabetes is that it is ''Silent Killer''.By the time patient is diagnosed to have diabetes,he/she is already affected with complications like diabetic nephropathy, retinopathy and neuropathy. It is specially worse in India, where various factors delay the diagnosis of diabetes compared to developed nations. Diabetic nephropathy and microalbuminuria are also strong predictors of cardiovascular and overall mortality in patients of diabetes,and hence are vital indicators in the patients. AIM: To find out prevalence of nephropathy in newly diagnosed Type 2 diabetes mellitus patients and other complications. METHODS:.In this cross-sectional study first 100 pts from PMCH Medicine OPD and IPD of age>20yrs of type2 diabetes mellitus during study period were taken and evaluated for HbA1c, urea, creatinine, Microalbuminuria, LVH, & blood sugar levels. RESULT: .It was found that 34 % of patients of type 2 diabetes mellitus have developed nephropathy at the time of detection.Among these 34 patients 18 have developed LVH and 13 out of 66 diabetic patients developed LVH.Result is significant with p<0.001.92% of newly diagnosed diabetic patients have HbA1c>7.5% (p=0.01 significant) CONCLUSION:. diabetic nephropathy is much larger than imagined in freshly diagnosed/new onset cases of DM type 2,especially compared to more developed nations.Microalbuminuria in patients with diabetes is a potential risk factor not only for kidney function impairment but also a marker for high risk of cardiovascular complications.

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